RESUMO
ABSTRACT Objectives To describe the retrieval spermatozoa technique for cryopreservation after death, including the proximal part of vas deferens. Material and Methods A 28-years old man, with previous history of infertility, who had died 12 hours before, was submitted to spermatozoa retrieval for cryopreservation, with surgical bilateral resection in bloc of the proximal part of vas deferens, testicle and epididymis. At the laboratory, by milking the epididymis and vas deferens, the extracted fluid was collected; also, three samples of each testicle parenchyma were also harvested. Results The fluid from the vas deferens showed spermatozoa, mostly with in situ motility. Testicular fragments also presented spermatozoa, mostly with small tail movements or immobile. Conclusion The inclusion of the proximal part of vas deferens during spermatozoa retrieval after death must be performed, since it contains high concentration of spermatozoa, and even in the presence of previous infertility, as was with this patient, it is possible to retrieve spermatozoa.
Assuntos
Humanos , Masculino , Adulto , Preservação do Sêmen/métodos , Criopreservação , Recuperação Espermática , Motilidade dos Espermatozoides , Ducto Deferente , EpididimoRESUMO
OBJECTIVES: To describe the retrieval spermatozoa technique for cryopreservation after death, including the proximal part of vas deferens. MATERIAL AND METHODS: A 28-years old man, with previous history of infertility, who died 12 hours ago, was submitted to spermatozoa retrieval for cryopreservation, with surgical bilateral resection in bloc of the proximal part of vas deferens, testicle and epididymis. At the laboratory, by milking the epididymis and vas deferens, the extracted fluid was collected; also, three samples of each testicle parenchyma were also harvested. RESULTS: The fluid from the vas deferens showed spermatozoa, mostly with in situ motility. Testicular fragments also presented spermatozoa, mostly with small tail movements or immobile. CONCLUSION: The inclusion of the proximal part of vas deferens during spermatozoa retrieval after death must be performed, since it contains high concentration of spermatozoa, and even in the presence of previous infertility, as was with this patient, it is possible to retrieve spermatozoa.
Assuntos
Criopreservação , Preservação do Sêmen/métodos , Recuperação Espermática , Adulto , Epididimo , Humanos , Masculino , Motilidade dos Espermatozoides , Ducto DeferenteRESUMO
As aderências peritoniais säo frequentes em cirurgias abdominais e pélvicas, podendo trazer, como consequência, esterilidade, dor pélvica crônica e obstruçäo intestinal. O objetivo deste estudo é avaliar a eficácia da membrana cirúrgica de polímero de tetrafluoretileno, utilizda como barreira cirúrgica intraperitoneal, para diminuir a formaçäo de aderências. Realizou-se estudo experimental com trinta ratos Wistar, fêmeas, adultos, nos quais, através de técnicas conhecidas para desenvolver modelos de aderências intraperitoniais, criaram-se condiçöes favoráveis para estudar uma barreira cirúrgicas...
Assuntos
Animais , Feminino , Aderências Teciduais , MembranasRESUMO
Os autores procuram demonstrar a eficácia do tratamento com análogo do GnRH (acetato de leuprolide) na endometriose experimental induzida em ratas. A induçao foi realizada em 80 fêmeas. OS animais do grupo tratado tiveram suas lesoes avaliadas com quatro, sete, nove e 12 semanas de tratamento. O volume de tecido endometrial sofreu uma diminuiçao significativa com o uso de droga. Conclui-se, assim, que o acetato de leuprolide faz reduçao das lesoes de forma significativa e que a técnica utilizada para a induçao da endometriose é eficaz.