RESUMO
OBJECTIVE: To show the results of Radioimmunodetection (RID) using Cuban monoclonal antibody (MoA) anti-egf-ior-egf-r3 labeled with 99mTc for the detection of primary tumors and/or metastases of head and neck epithelial malignant tumors. MATERIAL AND METHODS: Thirteen patients aged from 16-62 years (mean: 54.8 years) with primary tumors and metastases in the regional cervical lymph nodes were retrospectively evaluated. The labelling dose was 1480-2220 MBq (40-60 mCi). Planar images were performed after 10 minutes and together with SPECT images after 18-24 hours following MoA administration. The matrix was 128 x 128. SPECT images were reconstructed using the Butterworth 4/16 filter. A positive result was defined when the lesions were visualized. RESULTS: RID localized 11 of the known primary tumors in the 13 patients studied. In the two other patients, in whom the primary tumor site was unknown, cervical lymph node metastases were found. The results of 3 of the RID were false negative but in the other 10, RID was able to localize the primary tumor and cervical lymph node metastases. Sensitivity was 77 % and the predictive positive value was 100 %. CONCLUSIONS: The results of this study show that the Cuban MoA ior-egf-r3 can be employed for RID of primary head and neck tumors and their metastases. The radiation dose is adequate for RID.
Assuntos
Anticorpos Monoclonais , Receptores ErbB/imunologia , Neoplasias de Cabeça e Pescoço/diagnóstico por imagem , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Humanos , Masculino , Pessoa de Meia-Idade , Cintilografia , Estudos RetrospectivosRESUMO
UNLABELLED: IOR C-5 is a G1 immunoglobulin type intact murine monoclonal antibody (MAb) that was developed in the Center of Molecular Immunology in Havana City, Cuba. In immunohistochemical studies, this demonstrated a significant affinity for the epithelial tissues so that it was used in a pilot clinical study to perform a radioimmunoscintigraphy of the colorectal primary tumors and their locoregional recurrences. It was labeled with 99mTc using the Schwarz method, with a > 95% performance. Planar images of the chest, abdomen and pelvis were performed at 10 minutes, 4-6 hours and 18-24 hours post-injection in the anterior and posterior projections and the SPECT was performed 4-6 hours and 18-24 hours post-injection of 1.85 GBq 99mTC. This study has aimed to verify in vivo the capacity of ior-C5 MAb to accumulate in the malignant colorectal lesions. ior-C5 accumulated in 5 out of the 7 patients who were studied and who were suffering from colorectal cancer or in whom there was suspicion of recurrence. There was a negative case of primary tumors, which was an adenocarcinoma in situ in a tubular-papillary adenoma. The second case with a negative radioimmunoscintigraphy was a true negative case. CONCLUSIONS: It can be concluded that even though the number of patients is quite low, ior-C5 fulfilled the expectations of recognizing the epitope expressed in colorectal tumors in an in vivo human environment.
Assuntos
Adenocarcinoma/diagnóstico por imagem , Anticorpos Monoclonais , Antígenos de Neoplasias/análise , Biomarcadores Tumorais/análise , Antígeno Carcinoembrionário/análise , Neoplasias Colorretais/diagnóstico por imagem , Imunoconjugados , Radioimunodetecção , Compostos Radiofarmacêuticos , Tecnécio , Tomografia Computadorizada de Emissão de Fóton Único , Adenocarcinoma/química , Adenoma/química , Adenoma/diagnóstico por imagem , Adulto , Idoso , Animais , Anticorpos Heterófilos/biossíntese , Anticorpos Monoclonais/imunologia , Antígenos de Neoplasias/imunologia , Biomarcadores Tumorais/imunologia , Antígeno CA-19-9/sangue , Antígeno Carcinoembrionário/imunologia , Carcinoma in Situ/química , Carcinoma in Situ/diagnóstico por imagem , Neoplasias Colorretais/química , Epitopos/análise , Epitopos/imunologia , Feminino , Humanos , Imunoglobulina G/imunologia , Masculino , Camundongos , Pessoa de Meia-Idade , Recidiva Local de Neoplasia/diagnóstico por imagem , Projetos PilotoRESUMO
The anti-carcinoembryonic antigen (CEA) antibody, BW 431/26 (Scintimun CEA, Behringwerke, Marburg, Germany ) labeled with technetium pertechnectate (99mTc), is an intact immunoglobulin G1, monoclonal antibody that has been used to image colorectal cancer. Planar and SPECT images of chest, abdomen and pelvis were performed at 10 minutes, 4-6 and 18-24 hours after the intravenous antibody injection. 44 patients were studied and the pathological antibody concentration localization by radioimmunoimaging (RI) were correlated with surgical, clinical and other imaging modality findings to validate the RI. The RI was positive in 29 patients and negative in the other 15 patients. The CEA and CA 19.9 were elevated in the serum of some patients with primary tumors or recurrence. The HAMA were determined in all the patients before and after the RI.
Assuntos
Anticorpos Monoclonais , Antígenos de Neoplasias/imunologia , Antígeno CA-19-9/imunologia , Antígeno Carcinoembrionário/imunologia , Carcinoma/diagnóstico por imagem , Neoplasias Colorretais/diagnóstico por imagem , Pertecnetato Tc 99m de Sódio , Tomografia Computadorizada de Emissão de Fóton Único , Adulto , Idoso , Animais , Antígenos de Neoplasias/análise , Antígeno CA-19-9/análise , Antígeno Carcinoembrionário/análise , Carcinoma/química , Neoplasias do Colo/diagnóstico por imagem , Neoplasias Colorretais/química , Estudos de Avaliação como Assunto , Feminino , Humanos , Masculino , Camundongos , Pessoa de Meia-Idade , Metástase Neoplásica/diagnóstico por imagem , Recidiva Local de Neoplasia/diagnóstico por imagem , Neoplasia Residual/diagnóstico por imagem , Valor Preditivo dos Testes , Radioimunodetecção , Neoplasias Retais/diagnóstico por imagem , Neoplasias do Colo Sigmoide/diagnóstico por imagem , Especificidade da EspécieRESUMO
A 57 year old female had a tubular gastric adenocarcinoma confirmed by endoscopy and radiologic studies. Echotomography of the abdomen revealed multiple adenopathies. Immunoscintigraphy with 99mTc labeled anti-CEA BW 431/26 monoclonal antibody was performed. Images obtained at 6 and 24 h showed a significant uptake at the primary lesion and at periaortic adenopathies both in conventional gamma-camera and single emission photon tomography. This immuno-scintigraphic technique may be helpful in the management of patients with gastric cancer.