RESUMO
Ulva ohnoi is a green macroalga with fast growth and high rates of nitrogen and phosphorus absorption. Recently, this species has been recorded in several places with record green tide formation in some of them. Using molecular tools, we herein report the first occurrence of this species in Brazil and demonstrate its potential for phytoremediation in typical environmental concentrations of Cd (0.625-15⯵gâ¯L-1). Similarly, the effects of physicochemical parameters (salinity and temperature) on the toxicity and uptake efficiency of this species were evaluated. Molecular analysis of two sequences (1141â¯bp) obtained corroborates another 34 sequences for U. ohnoi obtained from GenBank. The addition of Cd in the medium affected photosynthetic parameters and reduced growth rate. U. ohnoi showed resistance to Cd when cultivated at 18⯰C, S15 and 18-25⯰C, S35, at concentrations between 0.625 and 2.5⯵g. L-1 of Cd; yet, positive growth rate was maintained. Dose-dependent accumulation was observed in all combinations of factors used with a maximum value of 4.20⯵gâ¯Cd per gram of dry seaweed at 15⯵g. L-1 of Cd at 18⯰C and S35. Maximum value of the concentration factor was 81.3⯱â¯1.1% of Cd added at the concentration of 0.625⯵g. L-1 to S15 and 18⯰C. Our results demonstrate the potential of using U. ohnoi in the phytoremediation of Cd in saltwater or brackish water.
Assuntos
Cádmio/toxicidade , Alga Marinha/efeitos dos fármacos , Ulva/efeitos dos fármacos , Poluentes Químicos da Água/toxicidade , Biodegradação Ambiental , Brasil , Cádmio/metabolismo , Relação Dose-Resposta a Droga , Nitrogênio/metabolismo , Fósforo/metabolismo , Fotossíntese/efeitos dos fármacos , Salinidade , Alga Marinha/metabolismo , Temperatura , Ulva/metabolismo , Poluentes Químicos da Água/metabolismoRESUMO
Absorption spectra are indicative of biological sample chemical composition and can be used as a basis for the construction of descriptive and predictive models for biotechnological screening or assays. In marine algae, chemical composition can vary due to species-specific differences in biochemistry, as well as intra-specific responses to unique environmental variables. Different indices (UVCi, UVB+Ai and PARi) were proposed and calculated to evaluate how photoprotective compounds vary in 18 species of Phaeophyceae. In addition, they were correlated to abiotic factors. Through this technique, seven main peaks were detected in the absorbing spectra of marine brown algal extracts. The highest photoprotective indices values were found in species collected in tropical areas, where higher solar radiation is observed compared to the southern Brazilian coast. Considering additional abiotic factors, water temperature and nitrate concentration were negatively correlated with UV indices. PARi's indices were positively affected by nitrate. All species collected on the Brazilian coast have absorption peaks in the region of phenolic compounds and carotenoids, suggesting that tropical marine brown macroalgae may have developed an effective antioxidant defense system, suggesting adaptation to environments characterized by high solar radiation. UVR/PAR indices congregated essential information to possible future biotechnological screening, facilitating selection of high priority species or sites, fostering actions to enhance alternative sustainable management strategies of coastal environments.