RESUMO
A survey of the Trypanosoma vivax genome was carried out by the genome sequence survey (GSS) approach resulting in 1,086 genomic sequences. A total of 455 high-quality GSS sequences were generated, consisting of 331 non-redundant sequences distributed in 264 singlets and 67 clusters in a total of 135.5 Kb of the T. vivax genome. The estimation of the overall G+C content, and the prediction of the presence of ORFs and putative genes were carried out using the Glimmer and Jemboss packages. Analysis of the obtained sequences was carried out by BLAST programs against 12 different databases and also using the Conserved Domain Database, InterProScan, and tRNAscan-SE. Along with the existing 23 T. vivax entries in the GenBank, the 32 putative genes predicted and the 331 non-redundant GSS sequences reported herein represent new potential markers for the development of PCRbased assays for specific diagnosis and typing of Trypanosoma vivax.
Assuntos
Genoma de Protozoário , Trypanosoma vivax/genética , Trypanosoma vivax/fisiologia , Algoritmos , Animais , Biologia Computacional , Bases de Dados Genéticas , Bases de Dados de Ácidos Nucleicos , Etiquetas de Sequências Expressas , Biblioteca Gênica , Genes de Protozoários/genética , Marcadores Genéticos , Fases de Leitura Aberta , Reação em Cadeia da Polimerase , Proteínas de Protozoários/fisiologia , RNA de Transferência/genética , Análise de Sequência de DNA , SoftwareRESUMO
Nauseas y vomitos, causas digestivas, causas extradigestivas, psicologicas y psiquiatricas, trastornos funcionales de las enfermedades gastricas, efectos de los farmacos sobre la funcion gastrica, sindrome disenterico, sindrome de retencion gastrica, abdomen agudo, anorexia nerviosa, bulimia, obesidad, trastornos de la alimentacion, anorexia nerviosa, bulimia nerviosa