RESUMO

Domestic and wild goats are very susceptible animals to predation, specially when pregnancy occurs. This study aimed to evaluate the use of goat fetal ovarian tissue for vitrification followed by xenotransplantation and fresh xenotransplantation in two immunosuppressed mice models (C57BL/6 SCID and Balb-C NUDE). Goat fetus ovaries were collected in slaughterhouses, divided into small cortical pieces and were destined for fresh xenotransplantation (FX) and cryopreservation followed by xenotransplantation (CX). Five recipients from each lineage were used for FX and 10 animals from each lineage for CX. The mice were euthanized after 65 postoperative days, and the transplants were collected for microscopic assessment. The blood plasma was collected for estradiol measurement. Independently of mice strain, all recipients presented complete estrus cycle in FX and 80% after CX groups. Follicles were observed at all development stages without morphological changes. The volume density and total vessel surface observed in the transplants were different (p <0.01) between groups. The estradiol levels in the recipients did not differ (p <0.05) among the treatments. Thus, it is possible to activate the preantral follicles in the ovaries of fetuses by optimizing germplasm utilization and conservation of domestic and endangered wild goats that are in predatory situations, undesirable drowning or accidental death, since provided conditions for xenotransplantation are performed.

RESUMO

Studies in relation to blood conservation and responses to transfusion are scarce for ruminants. We evaluated the clinical manifestations of sheep that received a single homologous transfusion of whole blood, focusing on transfusion reactions. Eighteen adult sheep were subjected to a single phlebotomy to withdraw 40% of the total blood volume, which was placed into CPDA-1 bags and then divided into G0, animals that received fresh blood, and G15 and G35, animals that received blood stored for 15 or 35 days, respectively. Clinical observations were recorded throughout the transfusion, whereas heart rate, respiratory rate, and rectal temperature were assessed at the following times: 24 hours after phlebotomy and before transfusion; 30 minutes, six, twelve, 24, 48, 72, and 96 hours and eight and 16 days after transfusion. All groups presented transfusion reactions, among which hyperthermia was the most frequent (50% of animals). Tachycardia occurred most frequently in the G35 animals (50% of them). During transfusion G35 animals presented more clinical manifestation (P < 0.05). Transfusion of fresh or stored total blood improved the blood volume, but transfusion reactions occurred, demonstrating that a single transfusion of fresh or stored blood can cause inflammatory and febrile nonhemolytic transfusion reactions in sheep.

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RESUMO

A brucelose é uma zoonose infecto-contagiosa causada por bactérias do gênero Brucella. Ela já foi diagnosticada em todos os Estados da federação. Porém, pouco se sabe em relação a sua prevalência nos rebanhos paraenses. Deste modo, o presente estudo objetiva determinar a ocorrência de animais soro reagentes à brucelose bovina, através de um estudo retrospectivo de resultados de exames realizados por laboratórios particulares em diferentes regiões do Estado. Foram selecionados três laboratórios de diagnóstico de brucelose localizados no nos municípios de Santarém, Novo Repartimento e Tomé-Açu. Os exames foram realizados em animais com idade superior a 24 meses. Obtiveram-se um total de 7.724 resultados de exames provenientes de 14 municípios. Para o diagnóstico da brucelose foi realizado o Teste de soro aglutinação com Antígeno Acidificado Tamponado (AAT). Os fatores de risco município, macrorregião, microrregião, categoria animal e padrão racial foram avaliados pelo teste do qui quadrado de Pearson, já os fatores sexo e histórico de vacinação do rebanho através do teste exato de Fisher, utilizando software estatístico Minitab. Foram observados 792 bovinos positivos, prevalência média de 10,25%. Em relação aos fatores de risco estudados, a prevalência foi maior em vacas e novilhas, quando comparadas aos touros, sendo conseqüentemente maior nas fêmeas em relação aos machos. Estes resultados indicam que a Brucelose está disseminada em todo o Estado do Pará, sendo observada uma alta prevalência desta enfermidade. Em vista dos resultados obtidos torna-se necessária a sensibilização de proprietários e profissionais quanto à importância do controle desta enfermidade.

Brucellosis is a zoonotic infectious disease caused by Brucella sp. Bovine brucellosis is present in over 128 countries. It is considered an endemic disease in Brazil and was diagnosed in all states. In relation to brucellosis, little is known about their prevalence in cattle herds from Pará state, Brazil. Thus, this study aims to determine the occurrence of cattle serum reagent to brucellosis by a retrospective study of the results of serological tests from private laboratories in different regions of from Pará state, Brazil. We selected three laboratories for the diagnosis of brucellosis located in the municipalities of Santarém, Novo Repartimento and Tomé-Açu. The animals tested for brucellosis were all older than 24 months. We obtained a total of 7,724 test results from 14 municipalities. For the diagnosis of brucellosis it was performed agglutination test with buffered acidified antigen. Qui-square test was performed to assess the possible risk factors for brucellosis prevalence in relation to macro and micro region, animal category and breed, and Fisher's exact test was applied for the factors sex and vaccination history of the herd. We observed 792 positive cattle, resulting in an average prevalence of 10.25%. The prevalence was higher in cows and heifers when compared to bulls, and consequently higher in females compared to males. Herds vaccinated against brucellosis showed lower prevalence in relation to properties without vaccination. These results indicate that brucellosis is widespread throughout the state of Pará, with a high prevalence of this disease. This results show the importance of convinces the cattle farmers and veterinaries of the importance of control this disease in the state.

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