RESUMO
We investigated the effects of viable, extended freeze-drying (EFD) or heat-killed (HK) Mycobacterium bovis bacillus Calmette-Guérin (BCG) in respiratory burst activity, gene expression of CYBB and NCF1 encoding components of the human phagocyte nicotinamide adenine dinucleotide (NADPH) oxidase, TLR2 expression, and in IL-10 and TNF-α cytokine production by human peripheral blood mononuclear cells (PBMCs). Viable BCG significantly inhibited TLR2 and CYBB gene expression, as well as superoxide release by human PBMC. All BCG stimuli augmented IL-10 release, but only HK BCG or viable BCG increased TNF-α release by PBMCs. Our studies show that viable BCG can impair the NADPH oxidase system activation and the TLR2 route in human PBMCs. As well, different BCG preparations can distinctly influence cytokine production by human PBMCs.
Assuntos
Adjuvantes Imunológicos/farmacologia , Vacina BCG/farmacologia , Leucócitos Mononucleares/imunologia , NADPH Oxidases/imunologia , Explosão Respiratória/imunologia , Receptor 2 Toll-Like/imunologia , Adulto , Ensaio de Imunoadsorção Enzimática , Liofilização/métodos , Humanos , Interleucina-10 , Leucócitos Mononucleares/enzimologia , Glicoproteínas de Membrana/imunologia , NADPH Oxidase 1 , NADPH Oxidase 2 , RNA/química , RNA/genética , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Receptor 2 Toll-Like/genética , Fator de Necrose Tumoral alfa/imunologiaRESUMO
"Pacu" (Piaractus mesopotamicus), a freshwater fish, was used with the purpose of studying its quality when smoked, salted and dried, and canned. Waste flour was also analysed. Fish from a fish farm of Piracicaba,SP was used. The intended final use was children's school meal. The results of the analysis of the final products for moisture, protein, lipids, ash and NaCl, in percent, were, respectively, 11.36; 52.53; 18.31; 17.76; 16.25 for the salted and dried fish; 70.25; 23.80; 2.69; 3.13; 1.17 for smoked fish; 62.19; 18.59; 14.34; 2.79; 1.29 for canned fish and 15.73; 42.94; 20.91; 20.42 (NaCl not available) for the waste flour. The smoked and the canned fishes were submitted to taste panels. For the canned product the results were: aspect in the can: 89% excellent and 11% good; aspect on plate: 56% excellent and 44% good; odor: 67% excellent and 33% good; taste: 56% excellent, 33% good and 11% regular and texture: 22% excellent, 56% good and 22% regular (none of the above got the not acceptable note). For the smoked fish the results were, aspect: 45% excellent and 55% good; odor: 35% excellent, 60% good and 5% regular; taste: 25% excellent, 55% good and 20% regular and texture: 50% excellent and 50% good (none got not acceptable grade).
RESUMO
"Pacu" (Piaractus mesopotamicus), a freshwater fish, was used with the purpose of studying its quality when smoked, salted and dried, and canned. Waste flour was also analysed. Fish from a fish farm of Piracicaba,SP was used. The intended final use was children's school meal. The results of the analysis of the final products for moisture, protein, lipids, ash and NaCl, in percent, were, respectively, 11.36; 52.53; 18.31; 17.76; 16.25 for the salted and dried fish; 70.25; 23.80; 2.69; 3.13; 1.17 for smoked fish; 62.19; 18.59; 14.34; 2.79; 1.29 for canned fish and 15.73; 42.94; 20.91; 20.42 (NaCl not available) for the waste flour. The smoked and the canned fishes were submitted to taste panels. For the canned product the results were: aspect in the can: 89% excellent and 11% good; aspect on plate: 56% excellent and 44% good; odor: 67% excellent and 33% good; taste: 56% excellent, 33% good and 11% regular and texture: 22% excellent, 56% good and 22% regular (none of the above got the not acceptable note). For the smoked fish the results were, aspect: 45% excellent and 55% good; odor: 35% excellent, 60% good and 5% regular; taste: 25% excellent, 55% good and 20% regular and texture: 50% excellent and 50% good (none got not acceptable grade).