RESUMO
Cannabidiol (CBD) is a natural cannabinoid present in the Cannabis sativa plant, widely prescribed as an anticonvulsant drug, especially for pediatric use. However, its effects on male reproduction are still little investigated. Therefore, the present study assessed the effects of CBD on the spermatogenesis and sperm quality. For this, twenty-one-day-old Swiss mice received CBD for 34 consecutive days by gavage at doses of either 15 or 30 mg/kg. Chronic exposure to CBD decreased the frequency of stages VII-VIII and XII of spermatogenesis and an increase in the frequency of stage IX were noted. Furthermore, the seminiferous epithelium height reduced at stage IX and increased at stage XII in both CBD-treated groups. There was a significant rise of sperm DNA damage, while no genotoxic effects were observed in leukocytes. The activities of superoxide dismutase and catalase decreased, while malondialdehyde levels increased in the sperm of mice treated with a higher dose of CBD. Mice exposed to 30 mg/kg of CBD showed a reduction in the mobile spermatozoa percentage and in curvilinear velocity, while straight line and average path velocity decreased in both treated groups. The number of acrosome-intact spermatozoa declined in the CBD 30 group, and the number of abnormal acrosomes raised in both CBD groups. On the other hand, the weight of reproductive organs, sperm count, and hormone levels were not affected by CBD treatment. These findings show that dysregulation of the endocannabinoid system by CBD can reduce sperm quality. The mechanisms responsible may be associated with disorders during spermatogenesis, especially during the final stages of nuclear remodelling and assembly of acrosome. However, changes in mitochondrial function, as well as the reduction on the antioxidant enzyme activities during epididymal transit, at least partly, may also be involved.
Assuntos
Canabidiol/toxicidade , Espermatozoides/efeitos dos fármacos , Acrossomo/efeitos dos fármacos , Animais , Dano ao DNA/efeitos dos fármacos , Peroxidação de Lipídeos/efeitos dos fármacos , Masculino , Camundongos , Motilidade dos Espermatozoides/efeitos dos fármacos , Espermatogênese/efeitos dos fármacos , Espermatozoides/patologiaRESUMO
Estuaries hold major economic potential due their strategic location, close to seas and inland waterways, thereby supporting intense economic activity. The increasing pace of human development in coastal deltas over the past five decades has also strained local resources and produced extensive changes across both social and ecological systems. The Amazon estuary is located in the Amazon Basin, North Brazil, the largest river basin on Earth and also one of the least understood. A considerable segment of the population living in the estuary is directly dependent on the local extraction of natural resources for their livelihood. Areas sparsely inhabited may be exploited with few negative consequences for the environment. However, recent and increasing pressure on ecosystem services is maximised by a combination of factors such as governance, currency exchange rates, exports of beef and forest products. Here we present a cross methodological approach in identifying the political frontiers of forest cover change in the estuary with consequences for ecosystem services loss. We used a combination of data from earth observation satellites, ecosystem service literature, and official government statistics to produce spatially-explicit relationships linking the Green Vegetation Cover to the availability of ecosystems provided by forests in the estuary. Our results show that the continuous changes in land use/cover and in the economic state have contributed significantly to changes in key ecosystem services, such as carbon sequestration, climate regulation, and the availability of timber over the last thirty years.