RESUMO

Production of periplasmic human interferon-gamma (hINF-gamma) and human interleukin-2 (hIL-2) by the Tat translocation pathway in Escherichia coli BL21-SI was evaluated. The expression was obtained using the pEMR vector which contains the Tat-dependent modified penicillin acylase signal peptide (mSPpac) driven by the T7 promoter. The mSPpac-hINF-gamma was processed and the protein was transported to periplasm. Up to 30.1% of hINF-gamma was found in the periplasmic soluble fraction, whereas only 15% of the mSPpac-hIL-2 was processed, but hIL-2 was not found in the periplasmic soluble fraction.

Assuntos

Escherichia coli/fisiologia , Interferon gama/metabolismo , Interleucina-2/metabolismo , Engenharia de Proteínas/métodos , Humanos , Interferon gama/química , Interferon gama/genética , Interleucina-2/química , Interleucina-2/genética , Periplasma/química , Proteínas Recombinantes/metabolismo , Solubilidade