RESUMO
Hepatitis C virus (HCV) infection in children was assessed by RT-nested PCR of the 5untranslated region (5UTR) of the viral genome combined with virus genotyping, performed by restriction fragment length polymorphism (RFLP). We analysed HCV infection in 64 children and in 9 HCV chronically infected mothers corresponding to 10 of them. Thirty two children were positive for serum HCV RNA as determined by RT-nested PCR. The viremia was analysed in consecutive samples of 25 children. Nine children (36 per cent) were always positive for HCV RNA, in 5 (20 per cent) a positive RT-nested PCR turned negative in subsequent samples, other 9 (36 per cent) showed alternating RT-nested PCR results and in 2 (8 per cent) the RT-nested PCR turned positive after an initial negative result. The HCV genotype distribution was studied in 27/32 children and in 9 mothers, and it was similar to that reported in the literature for adult and pediatric patients in our country. Genotype 1 was predominant in our population. HCV genotype did not change in the same patient during the time of this study. HCV genotype was the same in mother-infant pairs. We could not establish a correlation between HCV genotype and vertical transmission of HCV. This study will be helpful to further analyze HCV behavior during pediatric infection and the hosts response in the initial stages of it. (Au)
Assuntos
Humanos , Masculino , Feminino , Lactente , Pré-Escolar , Criança , Adolescente , Adulto , Hepatite C/genética , Hepacivirus/genética , Hepatite C/transmissão , Genótipo , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Seguimentos , Transmissão Vertical de Doenças Infecciosas , RNA Viral/sangue , Polimorfismo de Fragmento de RestriçãoRESUMO
Hepatitis C virus (HCV) infection in children was assessed by RT-nested PCR of the 5'untranslated region (5'UTR) of the viral genome combined with virus genotyping, performed by restriction fragment length polymorphism (RFLP). We analysed HCV infection in 64 children and in 9 HCV chronically infected mothers corresponding to 10 of them. Thirty two children were positive for serum HCV RNA as determined by RT-nested PCR. The viremia was analysed in consecutive samples of 25 children. Nine children (36 per cent) were always positive for HCV RNA, in 5 (20 per cent) a positive RT-nested PCR turned negative in subsequent samples, other 9 (36 per cent) showed alternating RT-nested PCR results and in 2 (8 per cent) the RT-nested PCR turned positive after an initial negative result. The HCV genotype distribution was studied in 27/32 children and in 9 mothers, and it was similar to that reported in the literature for adult and pediatric patients in our country. Genotype 1 was predominant in our population. HCV genotype did not change in the same patient during the time of this study. HCV genotype was the same in mother-infant pairs. We could not establish a correlation between HCV genotype and vertical transmission of HCV. This study will be helpful to further analyze HCV behavior during pediatric infection and the host's response in the initial stages of it.
Assuntos
Humanos , Masculino , Feminino , Lactente , Pré-Escolar , Criança , Adolescente , Adulto , Hepacivirus , Hepatite C , Seguimentos , Genótipo , Hepatite C , Transmissão Vertical de Doenças Infecciosas , Polimorfismo de Fragmento de Restrição , Reação em Cadeia da Polimerase Via Transcriptase Reversa , RNA ViralRESUMO
HCV genomic characterization was performed by nucleotide sequence analysis (n=50) combined with restriction fragment length polymorphism (RFLP) of the 5UTR region in 82 isolates coresponding to different Argentine groups. Genotype 1 was detected in 70.7 percent of the samples (58 out of 82), genotype 2 in 21.9 percent (18 of 82) and genotypes 3 in the remaining 6 sera (7.3 percent). HCV ib subtype contributed with 35.3 percent to the whole population studied (29 of 82) and was detected in 6 out of 21 sporadic cases. Besides their epidemiological significance, these results should be taken into account when future vaccines are considered on the basis of geographical HCV genotypic prevalence. (AU)
Assuntos
Adulto , Pessoa de Meia-Idade , Pré-Escolar , Criança , Idoso , Feminino , Humanos , Adolescente , Hepacivirus/genética , Polimorfismo de Fragmento de Restrição , Filogenia , Hepatite C Crônica/sangue , Sequência de Bases , Análise de Sequência de RNA , Reação em Cadeia da Polimerase , Hepatite D Crônica , Genótipo , Argentina , Fatores de RiscoRESUMO
HCV genomic characterization was performed by nucleotide sequence analysis (n=50) combined with restriction fragment length polymorphism (RFLP) of the 5'UTR region in 82 isolates coresponding to different Argentine groups. Genotype 1 was detected in 70.7 percent of the samples (58 out of 82), genotype 2 in 21.9 percent (18 of 82) and genotypes 3 in the remaining 6 sera (7.3 percent). HCV ib subtype contributed with 35.3 percent to the whole population studied (29 of 82) and was detected in 6 out of 21 sporadic cases. Besides their epidemiological significance, these results should be taken into account when future vaccines are considered on the basis of geographical HCV genotypic prevalence.