RESUMO
PURPOSE: To evaluate the effect of a new cross-linked hyaluronan (NCHA) gel on healing of the staple line in an experimental sleeve gastrectomy. METHODS: Eighteen rats were randomly divided into three groups. The control group (n = 6) received no medication. In the saline group (n = 6) and NCHA gel group (n = 6), saline and NCHA gel were respectively administered onto the staple line and intraperitoneally into the abdominal cavity after the standard stapling procedure. RESULTS: The fibroblast activity and collagen deposition were significantly higher in the NCHA gel group than in the control group (p = 0.00, p = 0.017) and saline group (p = 0.004, p = 0.015). The tissue hydroxyproline protein level was significantly higher in the NCHA gel group than in the control group (p = 0.041). Adhesion formation was significantly lower in the NCHA gel group than in the control and saline groups (p = 0.015, p = 0.041). CONCLUSIONS: New cross-linked hyaluronan gel could be an effective approach to improve staple line wound healing and prevent potential leakage after sleeve gastrectomy. Moreover, NCHA gel helps to prevent adhesion formation without compromising healing of the staple line.
Assuntos
Gastrectomia/métodos , Ácido Hialurônico/farmacologia , Grampeamento Cirúrgico/instrumentação , Cicatrização/efeitos dos fármacos , Animais , Reagentes de Ligações Cruzadas/farmacologia , Modelos Animais de Doenças , Feminino , Obesidade/cirurgia , Complicações Pós-Operatórias/prevenção & controle , Distribuição Aleatória , Ratos , Ratos Sprague-Dawley , Aderências Teciduais/prevenção & controleRESUMO
Purpose: To evaluate the effect of a new cross-linked hyaluronan (NCHA) gel on healing of the staple line in an experimental sleeve gastrectomy.Methods: Eighteen rats were randomly divided into three groups. The control group (n = 6) received no medication. In the saline group (n = 6) and NCHA gel group (n = 6), saline and NCHA gel were respectively administered onto the staple line and intraperitoneally into the abdominal cavity after the standard stapling procedure.Results: The fibroblast activity and collagen deposition were significantly higher in the NCHA gel group than in the control group (p = 0.00, p = 0.017) and saline group (p = 0.004, p = 0.015). The tissue hydroxyproline protein level was significantly higher in the NCHA gel group than in the control group (p = 0.041). Adhesion formation was significantly lower in the NCHA gel group than in the control and saline groups (p = 0.015, p = 0.041).Conclusions: New cross-linked hyaluronan gel could be an effective approach to improve staple line wound healing and prevent potential leakage after sleeve gastrectomy. Moreover, NCHA gel helps to prevent adhesion formation without compromising healing of the staple line.(AU)
Assuntos
Animais , Feminino , Ratos , Ácido Hialurônico/análogos & derivados , Ácido Hialurônico/uso terapêutico , Gastrectomia , Grampeamento Cirúrgico , Cicatrização , Aderências Teciduais , Modelos Animais de Doenças , Ratos Sprague-DawleyRESUMO
Abstract Purpose: To evaluate the effect of a new cross-linked hyaluronan (NCHA) gel on healing of the staple line in an experimental sleeve gastrectomy. Methods: Eighteen rats were randomly divided into three groups. The control group (n = 6) received no medication. In the saline group (n = 6) and NCHA gel group (n = 6), saline and NCHA gel were respectively administered onto the staple line and intraperitoneally into the abdominal cavity after the standard stapling procedure. Results: The fibroblast activity and collagen deposition were significantly higher in the NCHA gel group than in the control group (p = 0.00, p = 0.017) and saline group (p = 0.004, p = 0.015). The tissue hydroxyproline protein level was significantly higher in the NCHA gel group than in the control group (p = 0.041). Adhesion formation was significantly lower in the NCHA gel group than in the control and saline groups (p = 0.015, p = 0.041). Conclusions: New cross-linked hyaluronan gel could be an effective approach to improve staple line wound healing and prevent potential leakage after sleeve gastrectomy. Moreover, NCHA gel helps to prevent adhesion formation without compromising healing of the staple line.
Assuntos
Animais , Feminino , Ratos , Cicatrização/efeitos dos fármacos , Grampeamento Cirúrgico/instrumentação , Gastrectomia/métodos , Ácido Hialurônico/farmacologia , Complicações Pós-Operatórias/prevenção & controle , Distribuição Aleatória , Aderências Teciduais/prevenção & controle , Ratos Sprague-Dawley , Reagentes de Ligações Cruzadas/farmacologia , Modelos Animais de Doenças , Obesidade/cirurgiaRESUMO
PURPOSE: To evaluate intraocular pressure (IOP) and extraocular orbital vessels with color Doppler ultrasound (CDU) and investigate the effects of obesity on retrobulbar blood flow. METHODS: Fifty-nine patients were included in this prospective study. Patients were divided into two groups according to body mass index: Group 1 (31 obese patients) and Group 2 (28 non-obese patients). IOP was measured with a Goldmann applanation tonometer, and CDU was used to evaluate the retrobulbar vessels. RESULTS: The mean IOP was 18 ± 6.68 mmHg in the obese group and 13.71 ± 1.60 mmHg in the control group (p<0.001). When the CDU values for the central retinal artery were compared between the groups, the pulsatility index was found to be significantly lower in the obese group than in the control group (p<0.001). When the CDU values for the ophthalmic artery (OA) were compared between the groups, the peak systolic velocity (p<0.001) and end-diastolic velocity (p=0.002) values were found to be significantly lower in the obese group than in the control group. CONCLUSIONS: Obese patients have a higher mean IOP and lower flow velocity than non-obese patients. Increased IOP together with decreased retrobulbar blood flow, particularly in obese individuals, may increase the risk of glaucoma development.
Assuntos
Pressão Intraocular/fisiologia , Obesidade/fisiopatologia , Artéria Oftálmica/fisiopatologia , Fluxo Sanguíneo Regional/fisiologia , Artéria Retiniana/fisiopatologia , Adulto , Fatores Etários , Índice de Massa Corporal , Estudos de Casos e Controles , Olho/irrigação sanguínea , Feminino , Glaucoma/etiologia , Hemodinâmica/fisiologia , Humanos , Masculino , Pessoa de Meia-Idade , Artéria Oftálmica/diagnóstico por imagem , Estudos Prospectivos , Valores de Referência , Artéria Retiniana/diagnóstico por imagem , Fatores de Risco , Fatores Sexuais , Estatísticas não Paramétricas , Tonometria Ocular , Ultrassonografia Doppler em CoresRESUMO
ABSTRACT Purpose: To evaluate intraocular pressure (IOP) and extraocular orbital vessels with color Doppler ultrasound (CDU) and investigate the effects of obesity on retrobulbar blood flow. Methods: Fifty-nine patients were included in this prospective study. Patients were divided into two groups according to body mass index: Group 1 (31 obese patients) and Group 2 (28 non-obese patients). IOP was measured with a Goldmann applanation tonometer, and CDU was used to evaluate the retrobulbar vessels. Results: The mean IOP was 18 ± 6.68 mmHg in the obese group and 13.71 ± 1.60 mmHg in the control group (p<0.001). When the CDU values for the central retinal artery were compared between the groups, the pulsatility index was found to be significantly lower in the obese group than in the control group (p<0.001). When the CDU values for the ophthalmic artery (OA) were compared between the groups, the peak systolic velocity (p<0.001) and end-diastolic velocity (p=0.002) values were found to be significantly lower in the obese group than in the control group. Conclusions: Obese patients have a higher mean IOP and lower flow velocity than non-obese patients. Increased IOP together with decreased retrobulbar blood flow, particularly in obese individuals, may increase the risk of glaucoma development.
RESUMO Objetivo: Avaliar a pressão intraocular (PIO) e vasos orbitários extraoculares com ultrassom Doppler colorido (UDC) e investigar os efeitos da obesidade sobre o fluxo sanguíneo retrobulbar. Métodos: Cinquenta e nove pacientes foram incluídos neste estudo prospectivo. Os pacientes foram divididos em dois grupos de acordo com o índice de massa corpo ral (IMC): Grupo 1 (31 pacientes obesos) e Grupo 2 (28 não obesos). As pressões intraoculares (PIOs) foram medidas com tonômetro de aplanação de Goldmann e o UDC foi utilizada para a avaliação de vasos retrobulbar. Resultados: As PIOs foram 18 ± 6,68 mmHg nos pacientes obesos e 13,71 ± 1,60 mmHg nos grupos controle (p<00,001). Quando os valores da artéria central da retina (ACR) foram comparados entre os grupos controle e obeso. O índice pulsátil (PI) foi marcadamente menor no grupo obeso (p<00,001). Quando os valores da artéria oftálmica (AO) dos grupos obesos e de controle foram comparados. Os valores de Velocidade do pico sistólico (PSV) (p<00,001) e velocidade diastólica final (EDV) (p=00,002) foram significativamente mais baixos no grupo obeso. Conclusão: Pacientes obesos têm maior PIO do que os pacientes não obesos. Os pacientes obesos têm diminuição nas velocidades de fluxo da AO. O aumento da PIO. Juntamente com a diminuição do fluxo sanguíneo retrobulbar, especialmente em indivíduos obesos, pode aumentar o risco de desenvolvimento de glaucoma.