RESUMO
PURPOSE: This study evaluates the effects of different toothpastes on the nanohardness and chemical compositions of restorative materials and dental surfaces. METHODS: Bovine enamel (n = 72) and dentin (n = 72) blocks were obtained and restored using RMGIC (n = 36) or CR (n = 36) to create the following surfaces: dentin adjacent to RMGIC (DRMGIC), enamel adjacent to RMGIC (ERMGIC), dentin adjacent to CR (DCR), and enamel adjacent to CR (ECR). After restoration, one hemiface of each specimen was coated with an acid-resistant varnish to facilitate the creation of control (C) and eroded (E) sides; the latter were achieved by erosion-abrasion cycles as follows: erosion with 1% citric acid: 5 days, four times for 2 min each day; 1% citric acid/abrasion, two times for 15 s, followed by immersion in a toothpaste slurry for 2 min. Toothpastes without fluoride (WF; n = 12), with sodium fluoride (NaF; n = 12), and with stannous fluoride (SnF2; n = 12) were used for RMGIC or CR. The specimens were analyzed for nanohardness (H), and chemical composition using energy-dispersive X-ray spectroscopy and Raman microscopy. The data were statistically analyzed using two-way repeated measures ANOVA and Tukey's test (α = 0.05). RESULTS: Lower H values were obtained with NaF for DRMGIC-C, with a statistically significant difference from the H value obtained with WF (p < 0.05). The calcium and phosphorus concentrations in DCR-E were significantly lower with WF than with the other types of toothpaste (p < 0.05). Fluoride-containing toothpastes are capable of preserving the main chemical components of the dentin adjacent to the restorative materials under erosive-abrasive conditions.
RESUMO
BACKGROUND: The purpose of this study was to investigate the effect of a novel dental bleaching technique with Violet LED on enamel color change, bond strength and hybrid layer nanomechanical properties in resin-dentin restoration, and dentin biostability. METHODS: A total of 125 bovine incisors were distributed into a control group, violet LED group (LED), and 35 % peroxide hydrogen bleaching gel (BLG) groups (n = 15). Three 45-minute sessions were performed for both bleaching procedures every week. Enamel color change (ΔE, ΔL, and Δb) was determined after every bleaching session. After color analysis, dentin was exposed for the resin-dentin bond strength analysis using microtensile test and evaluation of the nanomechanical properties at the hybrid layer (nanohardness). While half of the specimens were tested immediately, the remaining were evaluated after 10,000 thermal cycles (TC). Thirty additional teeth were used to investigate dentin ultimate tensile strength (UTS) after the bleaching treatments. UTS was evaluated before and after an enzymatic challenge. Two-way repeated measures ANOVA and Tukey's post-test were used for the statistical analysis (α = 0.05). RESULTS: Enamel bleaching effect was observed in the LED and BLG groups with significant alterations in the ΔE, ΔL, and Δb in the BLG group. No difference was observed in the resin-dentin bond strength among the groups (p > 0.05), however, TC negatively affected the bond strength values for all the groups. Nanomechanical properties ââremained unchanged when comparing immediate and after TC results (p > 0.05). Bleaching with BLG reduced significantly the dentin UTS, while all groups showed major decrease in UTS after the enzymatic challenge. CONCLUSIONS: Although violet LED was able to promote a bleaching effect, less color changes was observed when compared to BLG. None of the bleaching techniques effected the resin-bond strength or the nanomechanics of the hybrid layer. Violet LED did negatively effect dentin biostability as observed for BLG and it may promote less changes to the organic content of dentin.
Assuntos
Fotoquimioterapia , Clareamento Dental , Animais , Bovinos , Colágeno , Resinas Compostas , Dentina , Fotoquimioterapia/métodos , Fármacos Fotossensibilizantes , Cimentos de ResinaRESUMO
Bacterial leaf spot (BLS), caused by Xanthomonas arboricola pv. pruni, is one of the most important diseases in Brazilian peach [Prunus persica (L.) Batsch] orchards and all over the world. The main objective of this study was to evaluate for BLS sensitivity of peach genotypes. Evaluations of thirty genotypes were carried out during the onset of the disease, for incidence, severity and defoliation, in field conditions. Pearson's correlations between the percentage of defoliation and leaf severity rating were performed. Genotypes 'Conserva 985', 'Conserva 871', 'Conserva 1129', and 'Tropic Snow', as resistance sources, and 'Conserva 1153', 'Bonão', 'Conserva 1125', and 'Atenas', as susceptible to BLS, were submitted to detached-leaf bioassay and greenhouse evaluation. The peach genotypes showed different reactions to the BLS, and none was immune to the pathogen. 'Conserva 985' and 'Conserva 1129' confirmed resistance responsiveness while 'Conserva 1153', 'Conserva 1125' and 'Atenas' were found susceptible for the detached-leaf bioassay.
A bacteriose foliar causada por Xanthomonas arboricola pv. pruni é uma das mais importantes doenças do pessegueiro [Prunus persica (L.) Batsch] no Brasil e no mundo. Avaliou-se a sensibilidade de genótipos de pessegueiro a X. arboricola pv. pruni. Trinta genótipos foram avaliados em campo, quanto à incidência, severidade e desfolha causada pela bactéria. Calculou-se a correlação entre desfolha e severidade da doença. A partir dos resultados obtidos em campo, foram selecionados quatro genótipos resistentes ('Conserva 985', 'Conserva 871', 'Conserva 1129' e 'Tropic Snow') e quatro suscetíveis ('Conserva 1153', 'Bonão', 'Conserva 1125' e 'Atenas') para serem novamente avaliados pelo bioensáio com folhas destacadas e em casa de vegetação. Os genótipos diferiram quanto a reação ao patógeno, não sendo observada imunidade. Confirmou-se a resistência para 'Conserva 985' e 'Conserva 1129' e a suscetibilidade para 'Conserva 1153', 'Conserva 1125' e 'Atenas', pelo bioensáio com folhas destacadas.
RESUMO
Bacterial leaf spot (BLS), caused by Xanthomonas arboricola pv. pruni, is one of the most important diseases in Brazilian peach [Prunus persica (L.) Batsch] orchards and all over the world. The main objective of this study was to evaluate for BLS sensitivity of peach genotypes. Evaluations of thirty genotypes were carried out during the onset of the disease, for incidence, severity and defoliation, in field conditions. Pearson's correlations between the percentage of defoliation and leaf severity rating were performed. Genotypes 'Conserva 985', 'Conserva 871', 'Conserva 1129', and 'Tropic Snow', as resistance sources, and 'Conserva 1153', 'Bonão', 'Conserva 1125', and 'Atenas', as susceptible to BLS, were submitted to detached-leaf bioassay and greenhouse evaluation. The peach genotypes showed different reactions to the BLS, and none was immune to the pathogen. 'Conserva 985' and 'Conserva 1129' confirmed resistance responsiveness while 'Conserva 1153', 'Conserva 1125' and 'Atenas' were found susceptible for the detached-leaf bioassay.
A bacteriose foliar causada por Xanthomonas arboricola pv. pruni é uma das mais importantes doenças do pessegueiro [Prunus persica (L.) Batsch] no Brasil e no mundo. Avaliou-se a sensibilidade de genótipos de pessegueiro a X. arboricola pv. pruni. Trinta genótipos foram avaliados em campo, quanto à incidência, severidade e desfolha causada pela bactéria. Calculou-se a correlação entre desfolha e severidade da doença. A partir dos resultados obtidos em campo, foram selecionados quatro genótipos resistentes ('Conserva 985', 'Conserva 871', 'Conserva 1129' e 'Tropic Snow') e quatro suscetíveis ('Conserva 1153', 'Bonão', 'Conserva 1125' e 'Atenas') para serem novamente avaliados pelo bioensáio com folhas destacadas e em casa de vegetação. Os genótipos diferiram quanto a reação ao patógeno, não sendo observada imunidade. Confirmou-se a resistência para 'Conserva 985' e 'Conserva 1129' e a suscetibilidade para 'Conserva 1153', 'Conserva 1125' e 'Atenas', pelo bioensáio com folhas destacadas.