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Med Chem ; 14(6): 556-562, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29332591

RESUMO

BACKGROUND: Chagas disease is caused by the protozoan parasite Trypanosoma cruzi. This illness is found mainly in 21 Latin American countries and an estimated 8 million people are infected worldwide. The unsatisfactory chemotherapy provokes severe toxicity and resistant strains. Medicinal plants constitute a promising source of new drugs and remedies against all kinds of disorders, mainly infectious diseases arousing interest worldwide. OBJECTIVE: The aim of this study is the isolation, structural identification and evaluation of the trypanocidal activity of samples present in the Excoecaria lucida Sw. leaves. METHODS: Total extract (TE) of E. lucida Sw. leaves was obtained by ethanol extract therefore fractionated sequentially with hexane, ethyl acetate and n-butanol, to obtain three phases: Hex, EA and But, respectively. Ellagic acid (EL1) was purified from both EA and But phases, while EL2; a 1:1 stigmasterol-3-O-ß-D-glucopyranoside plus sitosterol-3-O-ß-D-glucopyranoside mixture was obtained from the Hex phase. Activity assays were performed using bloodstream and intracellular forms of T. cruzi and cytotoxicity assays using L929 fibroblasts. RESULTS: The EL1 and EL2 samples were more active against bloodstream trypomastigote forms with EC50 of 53.0±3.6 and 58.2±29.0 µg/mL, respectively; at 100 µg/mL. These samples also showed 70% of inhibition of L929 cells infection. Toxicity assays demonstrated that after 96 h of treatment only the fractions Hex and EA presented detectable cytotoxicity. CONCLUSION: Ellagic acid, stigmasterol-3-O-ß-D-glucopyranoside and sitosterol-3-O-ß-Dglucopyranoside are reported for the first time in E. lucida Sw. leaves as well as their biological activity studies supporting further investigations for Chagas disease treatment.


Assuntos
Extratos Vegetais/farmacologia , Tripanossomicidas/farmacologia , 1-Butanol/química , Acetatos/química , Animais , Ácido Elágico/isolamento & purificação , Ácido Elágico/farmacologia , Ácido Elágico/toxicidade , Euphorbiaceae/química , Fibroblastos/efeitos dos fármacos , Fibroblastos/microbiologia , Glucosídeos/isolamento & purificação , Glucosídeos/farmacologia , Glucosídeos/toxicidade , Hexanos/química , Camundongos , Extratos Vegetais/isolamento & purificação , Extratos Vegetais/toxicidade , Folhas de Planta/química , Sitosteroides/isolamento & purificação , Sitosteroides/farmacologia , Sitosteroides/toxicidade , Estigmasterol/análogos & derivados , Estigmasterol/isolamento & purificação , Estigmasterol/farmacologia , Estigmasterol/toxicidade , Tripanossomicidas/isolamento & purificação , Tripanossomicidas/toxicidade , Trypanosoma cruzi/efeitos dos fármacos
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