RESUMO
La transglutaminasa tisular (tTG) se conoce como el autoantígeno más específico en pacientes con enfermedad celíaca. Por ello, nuestro objetivo fue estandarizar un ensayo ELISA para detectar anticuerpos específicos anti-tTG en pacientes con dicha patología. Se purificó la tTG a partir de hígado de cobayo y compararla con la tTG de una casa comercial, comprobando su eficacia en la inducción de anticuerpos en conejos inmunizados. La tTG procesada en el laboratorio se purificó unas 100 veces respecto al extracto original, conservando una elevada actividad tTG; además se demostró que la tTG purificada estaba en condiciones más puras que la de la casa comercial. El método ELISA para detección de anticuerpos IgA anti tTG se probó con ambas tTG (purificada y comercial) en 5 pacientes celíacos, 15 controles de diagnóstico diferencial y 8 controles sanos. A pesar de lo bajo de la muestra se observó una diferencia significativa (p=0.019) en el grupo de celíacos respecto a los sanos empleando la tTG purificada, no así en los otros grupos ni con la tTG comercial. La tTG purificada es mejor con relación a los resultados obtenidos con la tTG comercial, a pesar de la ausencia de estandarización del ensayo por la baja muestra de pacientes con enfermedad celíaca activa
Assuntos
Adulto , Criança , Adolescente , Pessoa de Meia-Idade , Doença Celíaca/diagnóstico , Doença Celíaca/etiologia , Ensaio de Imunoadsorção Enzimática , Transglutaminases/análise , Gastroenterologia , VenezuelaRESUMO
Ajoene, (E, Z) -4, 5, 9-trithiadeca-1, 6, 11-triene 9 oxide, is a compound originally isolated from ethanolic extracts of garlic that impairs platelet aggregation by inhibiting the functional exposure of platelet integrins GPIIb/IIIa. In vitro, Ajoene is toxic for several tumoral cell lines, and exert an antiproliferative effect on T. cruzi and murine malaria parasites. Here we show that Ajoene strongly inhibited the proliferation induced in human lymphocytes by the mitogens phytohemagglutinin (PHA), phorbol myristate acetate (PMA) and anti-CD3, and the capping formation induced in B lymphocytes by anti-IgM antibodies. On macrophages, Ajoene was also found to partially inhibit the lypopolysaccharide-induced production of Tumor Necrosis Factor (TNF), and to decrease the phagocytic activity of thioglycolate-elicited mouse peritoneal macrophages for IgG-opsonized, human erythrocytes. Ajoene also partially prevented the lytic effect of human and rabbit TNF on Actinomycin D-treated WEHI 164 cells. These results strongly suggest that Ajoene is a potent modulator of membrane-dependent functions of immune cells.
Assuntos
Dissulfetos/farmacologia , Linfócitos/efeitos dos fármacos , Macrófagos Peritoneais/efeitos dos fármacos , Extratos Vegetais/farmacologia , Inibidores da Agregação Plaquetária/farmacologia , Animais , Divisão Celular/efeitos dos fármacos , Divisão Celular/imunologia , Sobrevivência Celular/efeitos dos fármacos , Sobrevivência Celular/imunologia , Dissulfetos/farmacocinética , Capeamento Imunológico/efeitos dos fármacos , Cinética , Ativação Linfocitária/efeitos dos fármacos , Linfócitos/citologia , Linfócitos/imunologia , Macrófagos Peritoneais/citologia , Macrófagos Peritoneais/fisiologia , Camundongos , Fagocitose/efeitos dos fármacos , Fagocitose/imunologia , Extratos Vegetais/farmacocinética , Sulfóxidos , Fator de Necrose Tumoral alfa/efeitos dos fármacos , Fator de Necrose Tumoral alfa/metabolismoRESUMO
Dietary experiments, performed in metabolic wards, gave rise to predictive regression equations relating changes of plasma cholesterol concentration to the intake of fatty acids of the diet. It has been established that polyunsaturated fatty acids diminish and most saturated fatty acids increase plasma cholesterol concentration. This information led to expect that dietary use of palm oil may induce an unfavorable plasma lipoprotein profile. This has not been the case as shown in various dietary experiments. The reasons for this discrepancy is discussed. The influence of palm oil enriched diets on prothrombotic variables show that platelets are not affected in their function during prolonged dietary intervention. It is important to continue research on the effects of palm oil based diet on plasma fibrinogen, factor VII. There is still discordant information in this field.
Assuntos
Arteriosclerose/etiologia , Gorduras na Dieta/farmacologia , Hipercolesterolemia/induzido quimicamente , Óleos de Plantas/farmacologia , Trombose/etiologia , Adulto , Animais , Arteriosclerose/prevenção & controle , Criança , Colesterol/sangue , Dieta Aterogênica , Gorduras na Dieta/administração & dosagem , Gorduras na Dieta/efeitos adversos , Fator VII/análise , Comportamento Alimentar , Feminino , Fibrinogênio/análise , Óleos de Peixe/farmacologia , Humanos , Ácido Linoleico , Ácidos Linoleicos/administração & dosagem , Ácidos Linoleicos/farmacologia , Lipoproteínas/sangue , Masculino , Óleo de Palmeira , Óleos de Plantas/administração & dosagem , Óleos de Plantas/efeitos adversos , Óleos de Plantas/química , Agregação Plaquetária/efeitos dos fármacos , Coelhos , População Rural , Óleo de Girassol , Trombose/prevenção & controle , Triglicerídeos/sangue , População Urbana , VenezuelaRESUMO
Ajoene (4,5,9-trithiadodeca-1,6,11-triene 9-oxide), a product initially isolated from extracts of garlic (Allium sativum), was tested for its antimalarial activity in vivo in a well-characterized murine model. A single ajoene dose of 50 mg/kg, on the day of infection, suppressed the development of parasitemia; there were no obvious acute toxic effects from the tested dose. The combination of ajoene (50 mg/kg) and chloroquine (4.5 mg/kg), given as a single dose on the day of the infection, completely prevented the subsequent development of parasitemia in treated mice.
Assuntos
Dissulfetos/farmacologia , Malária/tratamento farmacológico , Extratos Vegetais/farmacologia , Plasmodium berghei , Animais , Cloroquina/farmacologia , Cloroquina/toxicidade , Dissulfetos/toxicidade , Sinergismo Farmacológico , Feminino , Malária/parasitologia , Camundongos , Camundongos Endogâmicos BALB C , Extratos Vegetais/toxicidade , Plasmodium berghei/efeitos dos fármacos , SulfóxidosRESUMO
Endemic Chediak-Higashi Syndrome occurs in a restricted geographic area (Pregonero, State of Táchira, Venezuela). Neutrophils from these patients were unable to digest Candida albicans in vitro, but showed normal or increased metabolic activities. This finding supports the view that the endemic syndrome is bona fide Chediak-Higashi Syndrome.
Assuntos
Síndrome de Chediak-Higashi/fisiopatologia , Neutrófilos/fisiologia , Síndrome de Chediak-Higashi/genética , Humanos , Valores de Referência , VenezuelaRESUMO
The authors present the result of an investigation on the use of cement and bentonite in the treatment of pancreatic fistulas that coexisted with permeable duct and which has been closed by the local application of said elements.