RESUMO

Background: Complete isolation of genders allows intense estrous induction and synchronization once rams are introduced in ewe flocks at the onset of the breeding season (BS). This management practice, defined as the male effect, results from a neuroendocrine process mediated by pheromones. The male effect is a straightforward procedure to induce estrous in noncycling ewes, but conditions for its use have not been fully explored. Thus, this study aimed to evaluate hormone levels and ovarian activity of postpartum ewes in anestrus which are subjected to the male effect under different male to female ratios.Material, Methods & Results: Pospartum females were selected according to body condition score and cyclicity status. Females were kept apart from males during 30 days at a distance of 10 m. Anestrus and ovulation were determined by P4 measures on days 10, 20 and 30 after isolation from males. After P4 concentration diagnosis, anestrus ewe (n = 99) were subjected to male to ewe ratios (MFR) of 1:20 (MFR20), 1:30 (MFR30) and 1:40 (MFR40). Santa Inês rams  (n = 3) of proven fertility were used. Three females of each group were randomly subjected to blood collection for LH concentration analysis. Ovarian activity was performed by ultrasonography after estrus manifestation in six ewe of each group. Estrus events were observed twice a day during the BS of 35 days, and estrus wer

RESUMO

Background: Complete isolation of genders allows intense estrous induction and synchronization once rams are introduced in ewe flocks at the onset of the breeding season (BS). This management practice, defined as the male effect, results from a neuroendocrine process mediated by pheromones. The male effect is a straightforward procedure to induce estrous in noncycling ewes, but conditions for its use have not been fully explored. Thus, this study aimed to evaluate hormone levels and ovarian activity of postpartum ewes in anestrus which are subjected to the male effect under different male to female ratios.Material, Methods & Results: Pospartum females were selected according to body condition score and cyclicity status. Females were kept apart from males during 30 days at a distance of 10 m. Anestrus and ovulation were determined by P4 measures on days 10, 20 and 30 after isolation from males. After P4 concentration diagnosis, anestrus ewe (n = 99) were subjected to male to ewe ratios (MFR) of 1:20 (MFR20), 1:30 (MFR30) and 1:40 (MFR40). Santa Inês rams  (n = 3) of proven fertility were used. Three females of each group were randomly subjected to blood collection for LH concentration analysis. Ovarian activity was performed by ultrasonography after estrus manifestation in six ewe of each group. Estrus events were observed twice a day during the BS of 35 days, and estrus wer

RESUMO

Background: Complete isolation of genders allows intense estrous induction and synchronization once rams are introduced in ewe flocks at the onset of the breeding season (BS). This management practice, defined as the male effect, results from a neuroendocrine process mediated by pheromones. The male effect is a straightforward procedure to induce estrous in noncycling ewes, but conditions for its use have not been fully explored. Thus, this study aimed to evaluate hormone levels and ovarian activity of postpartum ewes in anestrus which are subjected to the male effect under different male to female ratios.Material, Methods & Results: Pospartum females were selected according to body condition score and cyclicity status. Females were kept apart from males during 30 days at a distance of 10 m. Anestrus and ovulation were determined by P4 measures on days 10, 20 and 30 after isolation from males. After P4 concentration diagnosis, anestrus ewe (n = 99) were subjected to male to ewe ratios (MFR) of 1:20 (MFR20), 1:30 (MFR30) and 1:40 (MFR40). Santa Inês rams  (n = 3) of proven fertility were used. Three females of each group were randomly subjected to blood collection for LH concentration analysis. Ovarian activity was performed by ultrasonography after estrus manifestation in six ewe of each group. Estrus events were observed twice a day during the BS of 35 days, and estrus wer

RESUMO

Background: Complete isolation of genders allows intense estrous induction and synchronization once rams are introduced in ewe flocks at the onset of the breeding season (BS). This management practice, defined as the male effect, results from a neuroendocrine process mediated by pheromones. The male effect is a straightforward procedure to induce estrous in noncycling ewes, but conditions for its use have not been fully explored. Thus, this study aimed to evaluate hormone levels and ovarian activity of postpartum ewes in anestrus which are subjected to the male effect under different male to female ratios.Material, Methods & Results: Pospartum females were selected according to body condition score and cyclicity status. Females were kept apart from males during 30 days at a distance of 10 m. Anestrus and ovulation were determined by P4 measures on days 10, 20 and 30 after isolation from males. After P4 concentration diagnosis, anestrus ewe (n = 99) were subjected to male to ewe ratios (MFR) of 1:20 (MFR20), 1:30 (MFR30) and 1:40 (MFR40). Santa Inês rams  (n = 3) of proven fertility were used. Three females of each group were randomly subjected to blood collection for LH concentration analysis. Ovarian activity was performed by ultrasonography after estrus manifestation in six ewe of each group. Estrus events were observed twice a day during the BS of 35 days, and estrus wer

RESUMO

Background: Complete isolation of genders allows intense estrous induction and synchronization once rams are introduced in ewe flocks at the onset of the breeding season (BS). This management practice, defined as the male effect, results from a neuroendocrine process mediated by pheromones. The male effect is a straightforward procedure to induce estrous in noncycling ewes, but conditions for its use have not been fully explored. Thus, this study aimed to evaluate hormone levels and ovarian activity of postpartum ewes in anestrus which are subjected to the male effect under different male to female ratios.Material, Methods & Results: Pospartum females were selected according to body condition score and cyclicity status. Females were kept apart from males during 30 days at a distance of 10 m. Anestrus and ovulation were determined by P4 measures on days 10, 20 and 30 after isolation from males. After P4 concentration diagnosis, anestrus ewe (n = 99) were subjected to male to ewe ratios (MFR) of 1:20 (MFR20), 1:30 (MFR30) and 1:40 (MFR40). Santa Inês rams  (n = 3) of proven fertility were used. Three females of each group were randomly subjected to blood collection for LH concentration analysis. Ovarian activity was performed by ultrasonography after estrus manifestation in six ewe of each group. Estrus events were observed twice a day during the BS of 35 days, and estrus wer

RESUMO

Background: Complete isolation of genders allows intense estrous induction and synchronization once rams are introduced in ewe flocks at the onset of the breeding season (BS). This management practice, defined as the male effect, results from a neuroendocrine process mediated by pheromones. The male effect is a straightforward procedure to induce estrous in noncycling ewes, but conditions for its use have not been fully explored. Thus, this study aimed to evaluate hormone levels and ovarian activity of postpartum ewes in anestrus which are subjected to the male effect under different male to female ratios.Material, Methods & Results: Pospartum females were selected according to body condition score and cyclicity status. Females were kept apart from males during 30 days at a distance of 10 m. Anestrus and ovulation were determined by P4 measures on days 10, 20 and 30 after isolation from males. After P4 concentration diagnosis, anestrus ewe (n = 99) were subjected to male to ewe ratios (MFR) of 1:20 (MFR20), 1:30 (MFR30) and 1:40 (MFR40). Santa Inês rams  (n = 3) of proven fertility were used. Three females of each group were randomly subjected to blood collection for LH concentration analysis. Ovarian activity was performed by ultrasonography after estrus manifestation in six ewe of each group. Estrus events were observed twice a day during the BS of 35 days, and estrus wer

RESUMO

This study had the objective to distinguish spermatic and seminal parameters in animals born during the dry (n=11) and rainy (n=11) seasons. In the rainy season, the spermatic concentration ranging from 0.90 to 2.7 x 109/mL, the progressive motility from 16.0% to 62.0%, the mass movement from 0.3 to 2.6, vigor from 1.1 to 2.8, major sperm defects of 18.3% to 3 % and minor sperm defects from 16.1%  to 3.4% . In the dry season, the spermatic concentration varied from 0.96 to 2.15 x 109/mL, the progressive motility from 35.4% to 64.1%, the mass movement form 1.7 to 3.1, vigor from 2.0 to 3.0, major sperm defects from 16.9% to 4.2% and minor sperm defects from 17.5% to 3.2%.  It was observed positive correlation between spermatic motility versus spermatic mass movement, spermatic motility versus spermatic vigor as well as a negative correlation between motility versus major sperm defects of the spermatozoa and between motility and spermatozoa smaller defects respectively, in the rainy and dry seasons. Based on these results we conclude that Boer male goats born in dry season have sexual retardation of three months in comparison to those born in the rainy station.  Key words:  Birth season, boer, goat, puberty, sperm morphology.

Este trabalho objetivou determinar motilidade, turbilhonamento, vigor, defeitos maiores e menores dos espermatozoides de caprinos da raça Boer durante a estação chuvosa (n = 11) e seca (n = 11). Na estação chuvosa, a concentração espermática variou de 0,90 ± 1,46 x 109/mL a 2,7 x 109/mL, a motilidade progressiva de 16,0% a 62,0%, o turbilhonamento de 0,3 a 2,6 , vigor de 1,1 a 2,8 , defeitos maiores de 18,3% a 3% e menores de 16,1% a 3,4%. Na estação seca, a concentração espermática variou de 0,96 x 109/mL a 2,15 x 109/mL, a motilidade progressiva de 35,4% a 64,1%, o turbilhonamento de 1,7 a 3,1, vigor de 2,0 a 3,0, defeitos maiores de 16,9% a 4,2% e menores de 17,5% a 3,2%. Foi registrada correlação positiva (P 0,05) entre motilidade espermática vs turbilhonamento espermático, respectivamente, para a estação chuvosa e seca, motilidade espermática vs vigor espermático bem como negativa entre motilidade progressiva vs defeitos maiores dos espermatozoides e entre motilidade progressiva e defeitos menores dos espermatozoides. Dessa forma, pode-se concluir que machos caprinos da raça Boer nascidos na estação seca têm atraso de três meses do início da maturidade sexual em comparação àqueles nascidos na estação chuvosa.  Palavras-chaveS: Boer, caprino, época do nascimento, espermograma.

RESUMO

This study had the objective to distinguish spermatic and seminal parameters in animals born during the dry (n=11) and rainy (n=11) seasons. In the rainy season, the spermatic concentration ranging from 0.90 to 2.7 x 109/mL, the progressive motility from 16.0% to 62.0%, the mass movement from 0.3 to 2.6, vigor from 1.1 to 2.8, major sperm defects of 18.3% to 3 % and minor sperm defects from 16.1%  to 3.4% . In the dry season, the spermatic concentration varied from 0.96 to 2.15 x 109/mL, the progressive motility from 35.4% to 64.1%, the mass movement form 1.7 to 3.1, vigor from 2.0 to 3.0, major sperm defects from 16.9% to 4.2% and minor sperm defects from 17.5% to 3.2%.  It was observed positive correlation between spermatic motility versus spermatic mass movement, spermatic motility versus spermatic vigor as well as a negative correlation between motility versus major sperm defects of the spermatozoa and between motility and spermatozoa smaller defects respectively, in the rainy and dry seasons. Based on these results we conclude that Boer male goats born in dry season have sexual retardation of three months in comparison to those born in the rainy station.  Key words:  Birth season, boer, goat, puberty, sperm morphology. 

Este trabalho objetivou determinar motilidade, turbilhonamento, vigor, defeitos maiores e menores dos espermatozoides de caprinos da raça Boer durante a estação chuvosa (n = 11) e seca (n = 11). Na estação chuvosa, a concentração espermática variou de 0,90 ± 1,46 x 109/mL a 2,7 x 109/mL, a motilidade progressiva de 16,0% a 62,0%, o turbilhonamento de 0,3 a 2,6 , vigor de 1,1 a 2,8 , defeitos maiores de 18,3% a 3% e menores de 16,1% a 3,4%. Na estação seca, a concentração espermática variou de 0,96 x 109/mL a 2,15 x 109/mL, a motilidade progressiva de 35,4% a 64,1%, o turbilhonamento de 1,7 a 3,1, vigor de 2,0 a 3,0, defeitos maiores de 16,9% a 4,2% e menores de 17,5% a 3,2%. Foi registrada correlação positiva (P 0,05) entre motilidade espermática vs turbilhonamento espermático, respectivamente, para a estação chuvosa e seca, motilidade espermática vs vigor espermático bem como negativa entre motilidade progressiva vs defeitos maiores dos espermatozoides e entre motilidade progressiva e defeitos menores dos espermatozoides. Dessa forma, pode-se concluir que machos caprinos da raça Boer nascidos na estação seca têm atraso de três meses do início da maturidade sexual em comparação àqueles nascidos na estação chuvosa.  Palavras-chaveS: Boer, caprino, época do nascimento, espermograma. 

RESUMO

This study was evaluated, by transretal ultrasound, the embryonic-fetal development from days 15th to 45th of gestation visualizing the first signs of gestation in 60 Sant Inês ewes. The identification of the earliest and the latest parameters of evaluation were performed between days 15th to 19th (16.7 ± 1,3) of pregnancy for intra-uterine fluid, . embryonic vesicle between days 16th to 22th (18.6 ± 1.4), embryo between 18th to 26th (22.8 ± 1.9), placentoms between 20th to 29th (25.1 ± 2.0), heartbeat between days 24th to 29th (25.9 ± 1.4), amniotic membrane between 24th to 32th (27.4 ± 1.8), head and upper body differentiation between 30th to 37th (33.4 ± 2.2), fetus movement between 30th to 38th (34.2 ± 2.0), umbilical cord between 32th to 39th (35.1 ± 1.5), button of the anterior and posterior members between 34th to 39th (36.7 ± 1.5) and ocular globe between days 39th to 43th (40.9 ± 1.2). It was concluded that the first signs of gestation can be identified as soon as day 15th days of gestation. However, it is wise diagnosing pregnancy after day 24th, when it is possible to visualize the fetus and the heart beat.

Avaliou-se, por ultra-sonografia transretal, o desenvolvimento embrionário-fetal de ovinos Santa Inês, identificando o dia da primeira visualização dos principais parâmetros da gestação de 60 ovelhas entre o 15 e o 45 dia de gestação. A identificação mais precoce e mais tardia dos parâmetros avaliados ocorreu entre os dias 15 e 19 (16,7 ± 1,3) da gestação para líquido intra-uterino, 16 e 22 (18,6 ± 1,4) para vesícula embrionária, 18 e 26 (22,8 ± 1,9) para embrião, 20 e 29 (25,1 ± 2,0) para placentomas, 24 e 29 (25,9 ± 1,4) para batimento cardíaco, 24 e 32 (27,4 ± 1,8) para membrana amniótica, 30 e 37 (33,4 ± 2,2) para diferenciação entre cabeça e tronco, 30 e 38 (34,2 ± 2,0) para movimento do feto, 32 e 39 (35,1 ± 1,5) para cordão umbilical, 34 e 39 (36,7 ± 1,5) para botão dos membros anteriores e posteriores e entre os dias 39 e 43 (40,9 ± 1,2) para globo ocular. Conclui-se que é possível identificar os primeiros sinais de gestação já no 15º dia, todavia, é prudente que o diagnóstico de gestação somente seja emitido a partir do 24º dia, quando é possível visualizar o embrião e seus batimentos cardíacos.

RESUMO

This study was evaluated, by transretal ultrasound, the embryonic-fetal development from days 15th to 45th of gestation visualizing the first signs of gestation in 60 Sant Inês ewes. The identification of the earliest and the latest parameters of evaluation were performed between days 15th to 19th (16.7 ± 1,3) of pregnancy for intra-uterine fluid, . embryonic vesicle between days 16th to 22th (18.6 ± 1.4), embryo between 18th to 26th (22.8 ± 1.9), placentoms between 20th to 29th (25.1 ± 2.0), heartbeat between days 24th to 29th (25.9 ± 1.4), amniotic membrane between 24th to 32th (27.4 ± 1.8), head and upper body differentiation between 30th to 37th (33.4 ± 2.2), fetus movement between 30th to 38th (34.2 ± 2.0), umbilical cord between 32th to 39th (35.1 ± 1.5), button of the anterior and posterior members between 34th to 39th (36.7 ± 1.5) and ocular globe between days 39th to 43th (40.9 ± 1.2). It was concluded that the first signs of gestation can be identified as soon as day 15th days of gestation. However, it is wise diagnosing pregnancy after day 24th, when it is possible to visualize the fetus and the heart beat.

Avaliou-se, por ultra-sonografia transretal, o desenvolvimento embrionário-fetal de ovinos Santa Inês, identificando o dia da primeira visualização dos principais parâmetros da gestação de 60 ovelhas entre o 15 e o 45 dia de gestação. A identificação mais precoce e mais tardia dos parâmetros avaliados ocorreu entre os dias 15 e 19 (16,7 ± 1,3) da gestação para líquido intra-uterino, 16 e 22 (18,6 ± 1,4) para vesícula embrionária, 18 e 26 (22,8 ± 1,9) para embrião, 20 e 29 (25,1 ± 2,0) para placentomas, 24 e 29 (25,9 ± 1,4) para batimento cardíaco, 24 e 32 (27,4 ± 1,8) para membrana amniótica, 30 e 37 (33,4 ± 2,2) para diferenciação entre cabeça e tronco, 30 e 38 (34,2 ± 2,0) para movimento do feto, 32 e 39 (35,1 ± 1,5) para cordão umbilical, 34 e 39 (36,7 ± 1,5) para botão dos membros anteriores e posteriores e entre os dias 39 e 43 (40,9 ± 1,2) para globo ocular. Conclui-se que é possível identificar os primeiros sinais de gestação já no 15º dia, todavia, é prudente que o diagnóstico de gestação somente seja emitido a partir do 24º dia, quando é possível visualizar o embrião e seus batimentos cardíacos.

RESUMO

The aim of this work was to evaluate the efficiency of different concentrations of a commercial FSH-p on the nuclear maturation of Bos indicus oocytes, cleavage and in vitro development of embryos until blastocyst stages. The oocytes were selected and transferred to the maturation medium (TCM 199/25 mM HEPES) supplemented with different concentrations of FSH-p (T1 = 10mg/m img border=0 width=32 height=32 id="_x0000_i1032" src="../../../../img/revistas/cr/v31n4/a14img01.gif"> ; T2 - 20mg/m img border=0 width=32 height=32 id="_x0000_i1033" src="../../../../img/revistas/cr/v31n4/a14img01.gif"> ; T3 - 40mg/m img border=0 width=32 height=32 id="_x0000_i1034" src="../../../../img/revistas/cr/v31n4/a14img01.gif">) and after 24 hours of incubation, at 39ºC in a moist 5% CO2 atmosphere. Some of the oocytes were removed and submitted to the analysis of nuclear maturation and the others were placed in the fertilization medium (mDM). After 18 hours of incubation, at the same atmosfhere condition mentioned above, the presumptive zygotes were transferred to the culture medium (KSOM) with a granulosa cells monolayer. The metaphase II, cleavage and blastocyst percentages were, respectively, 81.8/62.0/17.6% (T1), 55.6/64.0/19.5% (T2) and 50.0/65.0/16.3% (T3). The statistic analysis showed that a lower percentage of oocyte (P £ 0.05) treated with 20mg/m img border=0 width=32 height=32 id="_x0000_i1035" src="../../../../img/revistas/cr/v31n4/a14img01.gif"> and 40mg/m img border=0 width=32 height=32 id="_x0000_i1036" src="../../../../img/revistas/cr/v31n4/a14img01.gif"> of FSH-p reached the metaphase II stage and that the cleavage and blastocyst rate do not differ among treatments (P ³ 0.05). The results allow to conclude that the addition of 20mg/m img border=0 width=32 height=32 id="_x0000_i1037" src="../../../../img/revistas/cr/v31n4/a14img01.gif"> and 40mg/m img border=0 width=32 height=32 id="_x0000_i1038" src="../../../../img/revistas/cr/v31n4/a14img01.gif"> of FSH-p to the culture medium interfere in the nuclear maturation process, however all tested concentrations may be used without apparent damage to the cleavage and subsequent embryonic development.

O objetivo deste trabalho foi avaliar a eficiência de diferentes concentrações de um FSH-p comercial sobre a maturação nuclear de oócitos Bos indicus, clivagem e desenvolvimento in vitro de embriões até estádios de blastocisto. Após seleção e transferência para o meio TCM 199/HEPES suplementado com diferentes concentrações de FSH-p (T1 = 10mg/m img border=0 width=32 height=32 id="_x0000_i1025" src="../../../../img/revistas/cr/v31n4/a14img01.gif"> ; T2 = 20mg/m img border=0 width=32 height=32 id="_x0000_i1026" src="../../../../img/revistas/cr/v31n4/a14img01.gif"> ; T3 = 40mg/m img border=0 width=32 height=32 id="_x0000_i1027" src="../../../../img/revistas/cr/v31n4/a14img01.gif">), os oócitos foram incubados, durante 24 horas, a 39ºC em atmosfera úmida contendo 5% de CO2. Parte dos oócitos foram retirados para análise da maturação nuclear e os demais foram transferidos para o meio de fecundação (mDM). Após 18 horas de incubação nas mesmas condições atmosféricas mencionadas para os oócitos, os presumíveis zigotos foram distribuídos no meio de desenvolvimento embrionário (KSOM) contendo monocamada de células da granulosa. As porcentagens de metáfase II, de clivagem e de blastocisto foram, respectivamente, de 81,8/62,5/17,6% (T1); 55,6/64,0/19,5% (T2) e 50,0/65,0/16,3% (T3). A análise estatística revelou que uma menor porcentagem (P £ 0,05) de oócitos tratados com 20mg/m img border=0 width=32 height=32 id="_x0000_i1028" src="../../../../img/revistas/cr/v31n4/a14img01.gif"> e 40mg/m img border=0 width=32 height=32 id="_x0000_i1029" src="../../../../img/revistas/cr/v31n4/a14img01.gif"> de FSH-p alcançou o estádio de metáfase II e que as taxas de clivagem e blastocisto não diferiram (P ³ 0,05) entre os tratamentos. Os resultados permitem concluir que a adição de 20mg/m img border=0 width=32 height=32 id="_x0000_i1030" src="../../../../img/revistas/cr/v31n4/a14img01.gif"> e 40mg/m img border=0 width=32 height=32 id="_x0000_i1031" src="../../../../img/revistas/cr/v31n4/a14img01.gif"> de FSH-p ao meio de cultura interfere no processo de maturação nuclear, mas todas as concentrações testadas podem ser utilizadas sem prejuízo aparente para a clivagem e o posterior desenvolvimento embrionário.

RESUMO

The purpose was to early diagnose the puerperal alterations by biochemistry measurement of aspartate aminotransferase (AST), total bilirubin (T8), and total cholesterol (TC). Eighty five Holstein half-bred females with age varying between four and nine years of age were used. From this total, 41 (48.2%) females did not exhibit puerperal disturbances (retained placenta and genital catarrh) and 44 (51.8%) had the puerperal period compromised. Hyperactivity of AST was evidentiated in 30.6% of the animal that exhibited physiological puerperium and in 30.6% of those which exhibited puerperal pathology, while 17.6% showed AST and normal puerperium and 21.2% evidentiated physiological AST and pathological puerperium. Hyperbilirubinaemia was seen in 36.5% of females with normal puerperium and in 37.6% of those that had a pathological puerperium, while 11.8% showed T8 and physiological puerperium and 14.1% had normal T8 and altered puerperium . . Hypercholesterolaemia was evidentiated in 29.4% of the animais with physiological puerperium and in 24.7% of those which exhibited pathological puerperium, while 18.8% showed TC and normal puerperium and 27.1% evidentiated puerperial disturbance and normal TC. The results lead to the conclusion that the analysis of the metabolic profile at the end of gestation does not turn viable the early diagnosis of puerperal alterations in halfbred dairy c

Com o objetivo de diagnosticar precocemente as alterações puerperais através da aferição bioquímica da aspartatoaminotransferase (AST), da bilirrubina total (8T) e do colesterol total (CT), utilizou-se 85 fêmeas mestiças da raça holandesa com idade variando entre quatro e nove anos. Deste total, 41 (48,2%) fêmeas não evidenciaram alterações puerperais (retenção de placenta e catarro genital) e 44 (51,8%) tiveram o decurso do puerpério comprometido. Hiperatividade da AST foi evidenciada em 30,6% dos animais que apresentaram puerpério fisiológico e em 30,6% dos que mostraram patologia puerperal, enquanto 17,6% mostraram AST e puerpério normais e 21,2% evidenciaram AST fisiológica e puerpério patológico. Hiperbilirrubinemia foi verificada em 36,5% das fêmeas com puerpério normal e em 37,6% das que tiveram puerpério patológico, enquanto 11,8% apresentaram 8T e puerpério fisiológicos e 14,1% tiveram 8T normal e puerpério alterado. Hipercolesterolemia foi evidenciada em 29,4% dos animais com puerpério fisiológico e em 24,7% dos que mostraram patologia puerperal, enquanto 18,8% mostraram CT e puerpério normais e 27,1% evidenciaram distúrbio puerperal e CT normal. Os resultados permitem a conclusão que a análise do perfil metabólico no final da gestação não viabiliza o diagnóstico precoce de alterações puerperais em vacas mestiças com aptidão leiteira.

RESUMO

Objetivando aumentar a eficiência dos rufiões no serviço de inseminação artificial de bovinos, foram testadas duas técnicas cirúrgicas para o preparo destes animais, sendo uma por fixação lateral da curvatura caudal da flexura sigmóide do pênis e a outra através da estenose da lâmina interna prepucial com anel de aço inoxidável. Foram utilizados 40 machos mestiços da espécie Zebu com idade entre 15 e 36 meses, sendo que 20 deles foram submetidos a técnica de fixação lateral da curvatura caudal da flexura sigmóide do pênis e 20 pela técnica da estenose da lâmina interna prepucial com anel de aço inoxidável. Para avaliação dos resultados, os animais foram colocados na presença de fêmeas em estro a partir do 15º dia da intervenção cirúrgica, durante um período mínimo de 3 meses. Os rufiões, quando testados, não apresentaram alteração da libido e mostraram-se incapazes de exteriorizar o pênis, observações que permitiram concluir que as técnicas descritas, além de serem rápidas e de fácil execução, podem ser utilizadas com eficiência na prática de preparo de rufiões bovinos

RESUMO

The objective was to test the efficiency of the association between GnRH, PGFp and temporary calf removal on the return to ciclicity. One hundred and fifty Nelore cows with calf on foot were randomly and equally alloted into different experimental groups and artificially inseminated (AI) 12 hours alter standing estrous, detected by teasers and visual observation. The females were initially treated with 0.25 mg of gonadorelin (G) or with 0.02 rng of buserelin (B) associating (C) or not (S) with temporary calf removal of 48 hours. The females that associated did not respond to one of these treatments in the interval of 14 days, received 0.25 mg of cloprostenol at the 15th day, with observation of estrous for more 120 hours. Alter administration of both GnRH, 30.0% (G+CDT), 20.0% (G+SDT), 40.0% (B+CDT) and 23.3% (B+SDT) exhibited estrous, with no difference observed between the groups. From the females that received cloprostenol, 40.0% (G+CDT), 29.2% (G+SDT), 77.8% (B+CDT) and 34.8% (B+SDT) responded to the treatment within 120 hours, with no difference observed among the groups. From ali the females treated with this estrous induction program, 73.3% (G+CDT), 43.3% (G+SDT), 86.7% (B+CDT), 50.0% (B+SDT) and 30.0% (Control) exhibited estrous in a period of 20 days, showing that the percentages of groups G+CDT and B+GDT were higher (P s 0,05) than the Control group, and that those 01

Objetivou-se testar a eficiência da associação entre GnRH, PGFp e desmame temporário sobre o restabelecimento da ciclicidade de vacas Nelore em anestro pós-parto. Foram utilizadas 150 fêmeas com cria ao pé, as quais foram aleatoriamente e equitativamente distribuídas em cinco grupos experimentais e artificialmente inseminadas (IA) após 12 horas do início do estro, detectado por rufiões e observação visual. As fêmeas foram inicialmente tratadas com 0,25 mg de gonadorelina (G) ou com 0,02 mg de buserelina (B) associando-se (C) ou não (S) o desmame temporário (DT) de 48 horas. As fêmeas que não responderam ao tratamento no período de 14 dias, receberam 0,25 mg de cloprostenol na submucosa vulvar no 150 dia, observando-se o estro por mais 120 horas. Após a administração de ambos os GnRH, 30,0% (G+CDT), 20,0% (G+SDT), 40,0% (B+CDT) e 23,3% (B+SDT) apresentaram estro, não se constatando diferença entre os grupos. Das fêmeas que receberam o cloprostenol, 40,0% (G+CDT), 29,2% (G+SDT), 77,8% (B+CDT) e 34,8% (B+SDT) responderam ao tratamento em 120 horas, não se registrando também diferença entre os grupos. De todas as fêmeas tratadas, 73,3% (G+CDT), 43,3% (G+SDT), 86,7% (B+CDT), 50,0% (B+SDT) e 30,0% (Controle) apresentaram estro no período de 20 dias, verificando-se que as porcentagens dos grupos G+CDT e B+CDT foram maiores (P :5 0,05) do que a do grupo Controle e que as dos grupos G+S

RESUMO

The objective was to evaluate the efficiency of three defferent doses of pFSH in the superovulation of thirty 5/8 Girolando cows, which were randomly and quantitatively distributed in three experimental groups (A = 300 UI, 8 = 500 UI and C = 700 UI). The superovulation took place between the ninth and twelth day of the estrus cycle using eight decreasing doses of pFSH, by intramuscular via, in intervals of 12 hours. At the same time of the filth administration of pFSH, the females received, by intramuscular via, 150 a2g of cloprostenol (pGFp). The artificial inseminations (AI) were performed at 12 and 24 hours after standing estrus and the embryos recovery, by transcervical via, between the sixth and eigth days alter the first AI. With the exception of a donor of group A, the other amimals showed estrus within 36 to 56 hours alter PGFp administration. The average values of the structure were 0.2±0.6 (A), 4.0±3.5 (8), and 0.8±1.3 (C) and the viable structures were 0.2±0.6 (A), 3.3±3.5 (8) and 0.5±O.9 (C), which represent 100% (A), 81.5% (8) and 64.7% (C) of embryonic viability. It was verified that the achieved results of group 8 were higher (P 0.05) than those of groups A and C, which lead to conclude that if the dose of 500 UI of pFSH is not ideal, at least it seems to be the most advisable to ne used in 5/8 Girolando breed.

Objetivou-se avaliar a eficiência de três diferentes doses de FSHp na superovulação de 30 vacas 5/8 Girolando, as quais foram aleatória e equitativamente distribuídas em três grupos experimentais (A = 300 UI, 8 = 500 UI e C = 700 UI). A superovulação foi iniciada entre o nono e o décimo segundo dia do ciclo estral através de oito doses decrescentes de FSHp, via intramuscular, a intervalos de 12 horas. Concomitantemente, com a quinta aplicação de FSHp, as fêmeas receberam, via intramuscular, 150 ~g de cloprostenol (PGF p). As inseminações artificiais (IA) foram efetuadas as 12 e 24 horas de iniciado o estro e as colheitas de embriões, por via transcervical, entre o sexto e o oitavo dia após a realização da primeira IA. Com exceção de uma doadora do grupo A, as demais apresentaram estro entre 36 e 56 horas após aplicação da PGFp. Os valores médios do total de estruturas foram de 0,2±0,6 (A), 4,0±3,5 (8) e 0,8±1,3 (C) e os de estruturas viáveis foram de 0,2±0,6 (A), 3,3±3,5 (8) e 0,5±0,9 (C), o que representa 100% (A), 81,5% (8) e 64,7% (C) de viabilidade embrionária. Constatou-se que os achados do grupo 8 foram superiores (P 0,05) aos dos grupos A e C, fato que permite a conclusão de que a dose de 500 UI de FSHp se não é a ideal, pelo menos indica ser a mais recomendável para ser utilizada na raça 5/8 Girolando.

RESUMO

The objective was to evaluate the efficiency of three defferent doses of pFSH in the superovulation of thirty 5/8 Girolando cows, which were randomly and quantitatively distributed in three experimental groups (A = 300 UI, 8 = 500 UI and C = 700 UI). The superovulation took place between the ninth and twelth day of the estrus cycle using eight decreasing doses of pFSH, by intramuscular via, in intervals of 12 hours. At the same time of the filth administration of pFSH, the females received, by intramuscular via, 150 a2g of cloprostenol (pGFp). The artificial inseminations (AI) were performed at 12 and 24 hours after standing estrus and the embryos recovery, by transcervical via, between the sixth and eigth days alter the first AI. With the exception of a donor of group A, the other amimals showed estrus within 36 to 56 hours alter PGFp administration. The average values of the structure were 0.2±0.6 (A), 4.0±3.5 (8), and 0.8±1.3 (C) and the viable structures were 0.2±0.6 (A), 3.3±3.5 (8) and 0.5±O.9 (C), which represent 100% (A), 81.5% (8) and 64.7% (C) of embryonic viability. It was verified that the achieved results of group 8 were higher (P 0.05) than those of groups A and C, which lead to conclude that if the dose of 500 UI of pFSH is not ideal, at least it seems to be the most advisable to ne used in 5/8 Girolando breed.

Objetivou-se avaliar a eficiência de três diferentes doses de FSHp na superovulação de 30 vacas 5/8 Girolando, as quais foram aleatória e equitativamente distribuídas em três grupos experimentais (A = 300 UI, 8 = 500 UI e C = 700 UI). A superovulação foi iniciada entre o nono e o décimo segundo dia do ciclo estral através de oito doses decrescentes de FSHp, via intramuscular, a intervalos de 12 horas. Concomitantemente, com a quinta aplicação de FSHp, as fêmeas receberam, via intramuscular, 150 ~g de cloprostenol (PGF p). As inseminações artificiais (IA) foram efetuadas as 12 e 24 horas de iniciado o estro e as colheitas de embriões, por via transcervical, entre o sexto e o oitavo dia após a realização da primeira IA. Com exceção de uma doadora do grupo A, as demais apresentaram estro entre 36 e 56 horas após aplicação da PGFp. Os valores médios do total de estruturas foram de 0,2±0,6 (A), 4,0±3,5 (8) e 0,8±1,3 (C) e os de estruturas viáveis foram de 0,2±0,6 (A), 3,3±3,5 (8) e 0,5±0,9 (C), o que representa 100% (A), 81,5% (8) e 64,7% (C) de viabilidade embrionária. Constatou-se que os achados do grupo 8 foram superiores (P 0,05) aos dos grupos A e C, fato que permite a conclusão de que a dose de 500 UI de FSHp se não é a ideal, pelo menos indica ser a mais recomendável para ser utilizada na raça 5/8 Girolando.

RESUMO

The objective was to test the efficiency of the association between GnRH, PGFp and temporary calf removal on the return to ciclicity. One hundred and fifty Nelore cows with calf on foot were randomly and equally alloted into different experimental groups and artificially inseminated (AI) 12 hours alter standing estrous, detected by teasers and visual observation. The females were initially treated with 0.25 mg of gonadorelin (G) or with 0.02 rng of buserelin (B) associating (C) or not (S) with temporary calf removal of 48 hours. The females that associated did not respond to one of these treatments in the interval of 14 days, received 0.25 mg of cloprostenol at the 15th day, with observation of estrous for more 120 hours. Alter administration of both GnRH, 30.0% (G+CDT), 20.0% (G+SDT), 40.0% (B+CDT) and 23.3% (B+SDT) exhibited estrous, with no difference observed between the groups. From the females that received cloprostenol, 40.0% (G+CDT), 29.2% (G+SDT), 77.8% (B+CDT) and 34.8% (B+SDT) responded to the treatment within 120 hours, with no difference observed among the groups. From ali the females treated with this estrous induction program, 73.3% (G+CDT), 43.3% (G+SDT), 86.7% (B+CDT), 50.0% (B+SDT) and 30.0% (Control) exhibited estrous in a period of 20 days, showing that the percentages of groups G+CDT and B+GDT were higher (P s 0,05) than the Control group, and that those 01

Objetivou-se testar a eficiência da associação entre GnRH, PGFp e desmame temporário sobre o restabelecimento da ciclicidade de vacas Nelore em anestro pós-parto. Foram utilizadas 150 fêmeas com cria ao pé, as quais foram aleatoriamente e equitativamente distribuídas em cinco grupos experimentais e artificialmente inseminadas (IA) após 12 horas do início do estro, detectado por rufiões e observação visual. As fêmeas foram inicialmente tratadas com 0,25 mg de gonadorelina (G) ou com 0,02 mg de buserelina (B) associando-se (C) ou não (S) o desmame temporário (DT) de 48 horas. As fêmeas que não responderam ao tratamento no período de 14 dias, receberam 0,25 mg de cloprostenol na submucosa vulvar no 150 dia, observando-se o estro por mais 120 horas. Após a administração de ambos os GnRH, 30,0% (G+CDT), 20,0% (G+SDT), 40,0% (B+CDT) e 23,3% (B+SDT) apresentaram estro, não se constatando diferença entre os grupos. Das fêmeas que receberam o cloprostenol, 40,0% (G+CDT), 29,2% (G+SDT), 77,8% (B+CDT) e 34,8% (B+SDT) responderam ao tratamento em 120 horas, não se registrando também diferença entre os grupos. De todas as fêmeas tratadas, 73,3% (G+CDT), 43,3% (G+SDT), 86,7% (B+CDT), 50,0% (B+SDT) e 30,0% (Controle) apresentaram estro no período de 20 dias, verificando-se que as porcentagens dos grupos G+CDT e B+CDT foram maiores (P :5 0,05) do que a do grupo Controle e que as dos grupos G+S

RESUMO

Objetivando aumentar a eficiência dos rufiões no serviço de inseminação artificial de bovinos, foram testadas duas técnicas cirúrgicas para o preparo destes animais, sendo uma por fixação lateral da curvatura caudal da flexura sigmóide do pênis e a outra através da estenose da lâmina interna prepucial com anel de aço inoxidável. Foram utilizados 40 machos mestiços da espécie Zebu com idade entre 15 e 36 meses, sendo que 20 deles foram submetidos a técnica de fixação lateral da curvatura caudal da flexura sigmóide do pênis e 20 pela técnica da estenose da lâmina interna prepucial com anel de aço inoxidável. Para avaliação dos resultados, os animais foram colocados na presença de fêmeas em estro a partir do 15º dia da intervenção cirúrgica, durante um período mínimo de 3 meses. Os rufiões, quando testados, não apresentaram alteração da libido e mostraram-se incapazes de exteriorizar o pênis, observações que permitiram concluir que as técnicas descritas, além de serem rápidas e de fácil execução, podem ser utilizadas com eficiência na prática de preparo de rufiões bovinos

RESUMO

The purpose was to early diagnose the puerperal alterations by biochemistry measurement of aspartate aminotransferase (AST), total bilirubin (T8), and total cholesterol (TC). Eighty five Holstein half-bred females with age varying between four and nine years of age were used. From this total, 41 (48.2%) females did not exhibit puerperal disturbances (retained placenta and genital catarrh) and 44 (51.8%) had the puerperal period compromised. Hyperactivity of AST was evidentiated in 30.6% of the animal that exhibited physiological puerperium and in 30.6% of those which exhibited puerperal pathology, while 17.6% showed AST and normal puerperium and 21.2% evidentiated physiological AST and pathological puerperium. Hyperbilirubinaemia was seen in 36.5% of females with normal puerperium and in 37.6% of those that had a pathological puerperium, while 11.8% showed T8 and physiological puerperium and 14.1% had normal T8 and altered puerperium . . Hypercholesterolaemia was evidentiated in 29.4% of the animais with physiological puerperium and in 24.7% of those which exhibited pathological puerperium, while 18.8% showed TC and normal puerperium and 27.1% evidentiated puerperial disturbance and normal TC. The results lead to the conclusion that the analysis of the metabolic profile at the end of gestation does not turn viable the early diagnosis of puerperal alterations in halfbred dairy c

Com o objetivo de diagnosticar precocemente as alterações puerperais através da aferição bioquímica da aspartatoaminotransferase (AST), da bilirrubina total (8T) e do colesterol total (CT), utilizou-se 85 fêmeas mestiças da raça holandesa com idade variando entre quatro e nove anos. Deste total, 41 (48,2%) fêmeas não evidenciaram alterações puerperais (retenção de placenta e catarro genital) e 44 (51,8%) tiveram o decurso do puerpério comprometido. Hiperatividade da AST foi evidenciada em 30,6% dos animais que apresentaram puerpério fisiológico e em 30,6% dos que mostraram patologia puerperal, enquanto 17,6% mostraram AST e puerpério normais e 21,2% evidenciaram AST fisiológica e puerpério patológico. Hiperbilirrubinemia foi verificada em 36,5% das fêmeas com puerpério normal e em 37,6% das que tiveram puerpério patológico, enquanto 11,8% apresentaram 8T e puerpério fisiológicos e 14,1% tiveram 8T normal e puerpério alterado. Hipercolesterolemia foi evidenciada em 29,4% dos animais com puerpério fisiológico e em 24,7% dos que mostraram patologia puerperal, enquanto 18,8% mostraram CT e puerpério normais e 27,1% evidenciaram distúrbio puerperal e CT normal. Os resultados permitem a conclusão que a análise do perfil metabólico no final da gestação não viabiliza o diagnóstico precoce de alterações puerperais em vacas mestiças com aptidão leiteira.

RESUMO

The efficiency of three monolayers (continuous lineage - Madin and Darby Bovine Kidney/ MDBK; primary lineage - bovine celisfrom uterus and oviduct) hás been tested to verify the celular specificity through development of two cells embryos until compact morulaes stage in a coculture system without continuous CO2 flow. The selected mouse embryos (n=343) were randomiy divided into three experimental groups andplaced in closed culture tubes mantained aí 37°C during 72 hours. After the co-culture period, the porcentages of compact morulaes were 87.7% in oviduct cells, 86.2% in uterus monolayer and 88.3% in MDBK cells. It was not observed significative difference between these results and it is possible to condude that celular specificity is not importam to enhance the In vitro development of Mus musculus embryos.

A eficiência de três monocamadas celulares (linhagem contínua - células Madin and Darby Bovine Kidney/ MDBK;linhagem primária - células de útero e de oviduto bovino) foi testada para verificar a existência de especificidade celular através do desenvolvimento de embriões, desde o estádio de duas células até o de mórula compacta, em um sistema de cocultura sem fluxo externo de CO2. Depois da seleçâo, os embriões (n = 343) foram aleatoriamente distribuídos em diferentes tubos de ensaio, os quais foram colocados a 37°C durante 72 horas. Após o período de cocultura, as porcentagens de mórulas compactas obtidas foram de 87,7% em células de oviduto, 86,2% na monocamada de células uterinas e 88,3% na de células MDBK. Não foi observada diferen- ça significativa entre esses valores e, por isso mesmo, conclui-se que a especificidade celular não é importante para o desenvolvimento in vitro de embriões Mus musculus.