RESUMO
Non-small cell lung cancer (NSCLC) accounts for the vast majority of cases of lung neoplasms. It is formed in multiple stages, with interactions between environmental risk factors and individual genetic susceptibility and with genes involved in the immune and inflammatory response paths, cell or genome stability, and metabolism, among others. Our objective was to evaluate the association between five genetic variants (IL-1A, NFKB1, PAR1, TP53, and UCP2) and the development of NSCLC in the Brazilian Amazon. The study included 263 individuals with and without lung cancer. The samples were analyzed for the genetic variants of NFKB1 (rs28362491), PAR1 (rs11267092), TP53 (rs17878362), IL-1A (rs3783553), and UCP2 (INDEL 45-bp), which were genotyped in PCR, followed by an analysis of the fragments, in which we applied a previously developed set of informative ancestral markers. We used a logistic regression model to identify differences in the allele and the genotypic frequencies among individuals and their association with NSCLC. The variables of gender, age, and smoking were controlled in the multivariate analysis to prevent confusion by association. The individuals that were homozygous for the Del/Del of polymorphism NFKB1 (rs28362491) (p = 0.018; OR = 0.332) demonstrate a significant association with NSCLC, which was similar to that observed in the variants of PAR1 (rs11267092) (p = 0.023; OR = 0.471) and TP53 (rs17878362) (p = 0.041; OR = 0.510). Moreover, the individuals with the Ins/Ins genotype of polymorphism IL-1A (rs3783553) demonstrated greater risk for NSCLC (p = 0.033; OR = 2.002), as did the volunteers with the Del/Del of UCP2 (INDEL 45-bp) (p = 0.031; OR = 2.031). The five polymorphisms investigated can contribute towards NSCLC susceptibility in the population of the Brazilian Amazon.
Assuntos
Carcinoma Pulmonar de Células não Pequenas , Neoplasias Pulmonares , Subunidade p50 de NF-kappa B , Receptor PAR-1 , Proteína Supressora de Tumor p53 , Proteína Desacopladora 2 , Humanos , Brasil/epidemiologia , Subunidade p50 de NF-kappa B/genética , Polimorfismo Genético , Receptor PAR-1/genética , Proteína Supressora de Tumor p53/genéticaRESUMO
In Brazil, Acute lymphoid leukemia (ALL) is the leading cause of cancer deaths in children and adolescents. Treatment toxicity is one of the reasons for stopping chemotherapy. Amerindian genomic ancestry is an important factor for this event due to fluctuations in frequencies of genetic variants, as in the NUDT15 and SLC22A1 genes, which make up the pharmacokinetic and pharmacodynamic pathways of chemotherapy. This study aimed to investigate possible associations between NUDT15 (rs1272632214) and SLC22A1 (rs202220802) gene polymorphism and genomic ancestry as a risk of treatment toxicities in patients with childhood ALL in the Amazon region of Brazil. The studied population consisted of 51 patients with a recent diagnosis of ALL when experiencing induction therapy relative to the BFM 2009 protocol. Our results evidenced a significant association of risk of severe infectious toxicity for the variant of the SLC22A1 gene (OR: 3.18, p = 0.031). Genetic ancestry analyses demonstrated that patients who had a high contribution of African ancestry had a significant protective effect for the development of toxicity (OR: 0.174; p = 0.010), possibly due to risk effects of the Amerindian contribution. Our results indicate that mixed populations with a high degree of African ancestry have a lower risk of developing general toxicity during induction therapy for ALL. In addition, individuals with the SLC22A1 variant have a higher risk of developing severe infectious toxicity while undergoing the same therapy.
Assuntos
Transportador 1 de Cátions Orgânicos , Leucemia-Linfoma Linfoblástico de Células Precursoras , Adolescente , População Negra , Criança , Humanos , Transportador 1 de Cátions Orgânicos/genética , Polimorfismo Genético , Leucemia-Linfoma Linfoblástico de Células Precursoras/tratamento farmacológico , Leucemia-Linfoma Linfoblástico de Células Precursoras/genética , Pirofosfatases/genéticaRESUMO
Lung cancer is one of the most frequent neoplasms in the world. Because it is a complex disease, its formation occurs in several stages, stemming from interactions between environmental risk factors, such as smoking, and individual genetic susceptibility. Our objective was to investigate associations between a UGT1A1 gene polymorphism (rs8175347) and lung cancer risk in an Amazonian population. This is a pilot study, case-controlled study, which included 276 individuals with cancer and without cancer. The samples were analyzed for polymorphisms of the UGT1A1 gene (rs8175347) and genotyped in PCR, followed by fragment analysis in which we applied a previously developed set of informative ancestral markers. We used logistic regression to identify differences in allelic and genotypic frequencies between individuals. Individuals with the TA7 allele have an increased chance of developing lung adenocarcinoma (p = 0.035; OR: 2.57), as well as those with related genotypes of reduced or low enzymatic activity: TA6/7, TA5/7, and TA7/7 (p = 0.048; OR: 8.41). Individuals with homozygous TA7/7 have an increased chance of developing squamous cell carcinoma of the lung (p = 0.015; OR: 4.08). Polymorphism in the UGT1A1 gene (rs8175347) may contribute as a risk factor for adenocarcinoma and lung squamous cell carcinoma in the population of the Amazon region.
Assuntos
Carcinoma de Células Escamosas , Glucuronosiltransferase , Neoplasias Pulmonares , Glucuronosiltransferase/genética , Humanos , Neoplasias Pulmonares/genética , Projetos Piloto , Polimorfismo Genético , Fatores de RiscoRESUMO
Gastric cancer (GC) is a multifactorial, complex, and aggressive disease with a prevalence of one million new cases and high global mortality. Factors such as genetic, epigenetic, and environmental changes contribute to the onset and progression of the disease. Identification of INDELs in miRNA and its target sites in current studies showed an important role in the development of cancer. In GC, miRNAs act as oncogenes or tumor suppressors, favoring important cancer pathways, such as cell proliferation and migration. This work aims to investigate INDELs in the coding region of miRNAs (hsa-miR-302c, hsa-miR-548AJ-2, hsa-miR-4274, hsa-miR-630, hsa-miR-516B-2, hsa-miR-4463, hsa-miR-3945, hsa-miR-548H_4, hsa-miR-920, has-mir-3171, and hsa-miR-3652) that may be associated with susceptibility and clinical variants of gastric cancer. For this study, 301 patients with GC and 145 individuals from the control group were selected from an admixed population in the Brazilian Amazon. The results showed the hsa-miR-4463, hsa-miR-3945, hsa-miR-548H_4, hsa-miR-920 and hsa-miR-3652 variants were associated with gastric cancer susceptibility. The hsa-miR-4463 was significantly associated with clinical features of GC such as diffuse gastric tumor histological type, "non-cardia" localization region, and early onset. Our findings indicated that INDELs could be potentially functional genetic variants for gastric cancer risk.
Assuntos
MicroRNAs , Neoplasias Gástricas , Humanos , Neoplasias Gástricas/patologia , Regulação Neoplásica da Expressão Gênica , MicroRNAs/genética , MicroRNAs/metabolismo , Oncogenes , Biomarcadores Tumorais/genéticaRESUMO
BACKGROUND: Sarcopenia is a disease characterized by progressive reduction in muscle mass and strength or function. Although it is known that sarcopenia may be associated with environmental factors, studies suggest the identification of genes related to skeletal muscle maintenance that explain the susceptibility to the disease. OBJECTIVE: To analyze the influence of NFkB1 gene polymorphism on susceptibility to sarcopenia in the elderly. METHODS: This is a case-control study, which included 219 elderly people, 74 elderly people with sarcopenia, and 145 without sarcopenia. Samples were analyzed for NFkB1 gene polymorphism (rs28362491), genotyped in PCR, and followed by fragment analysis. To avoid misinterpretation due to population substructure, we applied a previously developed set of 61 informative ancestral markers that were genotyped by multiplex PCR. We used logistic regression to identify differences in genotypic frequencies between elderly people with and without sarcopenia. RESULTS: It was observed that the NFkB1 gene polymorphism presented frequencies of 24%, 50%, and 26% for the genotype DEL/DEL, DEL/INS, and INS/INS, respectively. Furthermore, elderly individuals with the INS/INS genotype had increased chances (p = 0.010; OR:2.943; 95%CI:1.301-6.654) for the development of sarcopenia. CONCLUSION: The INDEL polymorphism of the NFkB1 gene (rs28362491) may influence the susceptibility to sarcopenia in the elderly in elderly people in the Amazon.
RESUMO
Frost events occur with a significant frequency in savannas of the Southern Hemisphere, especially in the Cerrados of Brazil. One of the main strategies to deal with such events is to invest in thick and dense bark, which can insulate internal branch tissues and protect buds, essential to ensure resprouting if frost damage causes plant canopy die-back. Such strategies may be fundamental to determine the persistence of savanna species in regions where low temperatures and frost events are recurrent. Here we describe bud protection and bark strategies of 53 woody species growing in typical savanna vegetation of central Brazil. In addition, we used an experimental approach exposing branches to 0 °C to measure temperature variation in internal branch tissue and test its relationship to bud protection and bark properties. We found that the majority of species (69%) showed medium to high bud protection against extreme temperatures; however, the degree of bud protection was not clearly related to bark properties, such as bark thickness and density. Bark density is a fundamental trait in determining protection against low temperatures (0 °C), since species with low bark density showed lower temperature variation in their internal branch tissues, independently of the bud protection degree. Bark properties and bud protection are two different (albeit related) strategies for the protection and persistence of savanna trees under extreme environmental temperatures and can explain ecological observations related to savanna tree responses after frost events.
Assuntos
Temperatura Baixa , Pradaria , Árvores , Brasil , Casca de Planta/anatomia & histologia , Casca de Planta/fisiologiaRESUMO
Estimates of different ancestral proportions in admixed populations are very important in population genetics studies, especially for the detection of population substructure effects in studies of case-control associations. Brazil is one of the most heterogeneous countries in the world, both from a socio-cultural and a genetic point of view. In this work, we investigated a previously developed set of 61 ancestry informative markers (AIM), aiming to estimate the proportions of four different ancestral groups (African, European, Native American and Asian) in Brazilian populations. To the best of our knowledge, this is the first study to use a set of AIM to investigate the genetic contribution of all four main parental populations to the Brazilian population, including Asian contribution. All selected markers were genotyped through multiplex PCR and capillary electrophoresis. The set was able to successfully differentiate the four ancestral populations (represented by 939 individuals) and identify their genetic contributions to the Brazilian population. In addition, it was used to estimate individual interethnic admixture of 1050 individuals from the Southeast region of Brazil and it showed that these individuals present a higher European ancestry contribution, followed by African, Asian and Native American ancestry contributions. Therefore, the 61 AIM set has proved to be a valuable tool to estimate individual and global ancestry proportions in populations mainly formed by these four groups. Our findings highlight the importance of using sets of AIM to evaluate population substructure in studies carried in admixed populations, in order to avoid misinterpretation of results.
Assuntos
Grupos Raciais/genética , Brasil , Eletroforese Capilar , Marcadores Genéticos , Genótipo , Humanos , Reação em Cadeia da PolimeraseRESUMO
The effects induced by Apis mellifera venom (AMV), melittin-free AMV, fraction with molecular mass < 10 kDa (F<10) or melittin in nociceptive and inflammatory pain models in mice were investigated. Subcutaneous administration of AMV (2, 4 or 6 mg/kg) or melittin-free AMV (1, 2 or 4 mg/kg) into the dorsum of mice inhibited both phases of formaldehyde-induced nociception. However, F<10 (2, 4 or 6 mg/kg) or melittin (2 or 3 mg/kg) inhibited only the second phase. AMV (4 or 6 mg/kg), but not F<10, melittin-free AMV or melittin, induced antinociception in the hot-plate model. Paw injection of AMV (0.05 or 0.10 mg), F<10 (0.05 or 0.1 mg) or melittin (0.025 or 0.050 mg) induced a nociceptive response. In spite of inducing nociception after paw injection, scorpion (Tityus serrulatus) or snake (Bothrops jararaca) venom injected into the dorsum of mice did not inhibit formaldehyde-induced nociception. In addition, AMV (6 mg/kg), but not F<10 (6 mg/kg) or melittin (3 mg/kg), inhibited formaldehyde paw oedema. Concluding, AMV, F<10 and melittin induce two contrasting effects: nociception and antinociception. AMV antinociception involves the action of different components and does not result from non-specific activation of endogenous antinociceptive mechanisms activated by exposure to noxious stimuli.
Assuntos
Venenos de Abelha/toxicidade , Inflamação/induzido quimicamente , Meliteno/toxicidade , Dor/induzido quimicamente , Análise de Variância , Animais , Formaldeído/toxicidade , Masculino , Camundongos , Atividade Motora/efeitos dos fármacos , Medição da DorRESUMO
Antimicrobial peptides are components of innate immunity that is the first-line defense against invading pathogens for a wide range of organisms. Here, we describe the isolation, biological characterization and amino acid sequencing of a novel neutral Glycine/Leucine-rich antimicrobial peptide from skin secretion of Leptodactylus pentadactylus named leptoglycin. The amino acid sequence of the peptide purified by RP-HPLC (C(18) column) was deduced by mass spectrometric de novo sequencing and confirmed by Edman degradation: GLLGGLLGPLLGGGGGGGGGLL. Leptoglycin was able to inhibit the growth of Gram-negative bacteria Pseudomonas aeruginosa, Escherichia coli and Citrobacter freundii with minimal inhibitory concentrations (MICs) of 8 microM, 50 microM, and 75 microM respectively, but it did not show antimicrobial activity against Gram-positive bacteria (Staphylococcus aureus, Micrococcus luteus and Enterococcus faecalis), yeasts (Candida albicans and Candida tropicalis) and dermatophytes fungi (Microsporum canis and Trichophyton rubrum). No hemolytic activity was observed at the 2-200 microM range concentration. The amino acid sequence of leptoglycin with high level of glycine (59.1%) and leucine (36.4%) containing an unusual central proline suggests the existence of a new class of Gly/Leu-rich antimicrobial peptides. Taken together, these results suggest that this natural antimicrobial peptide could be a tool to develop new antibiotics.
Assuntos
Anti-Infecciosos/química , Peptídeos Catiônicos Antimicrobianos/química , Ranidae/metabolismo , Pele/química , Sequência de Aminoácidos , Animais , Anti-Infecciosos/isolamento & purificação , Anti-Infecciosos/farmacologia , Peptídeos Catiônicos Antimicrobianos/isolamento & purificação , Peptídeos Catiônicos Antimicrobianos/farmacologia , Bactérias/efeitos dos fármacos , Feminino , Fungos/efeitos dos fármacos , Glicina/química , Hemólise/efeitos dos fármacos , Humanos , Indicadores e Reagentes , Leucina/química , Masculino , Espectrometria de Massas , Testes de Sensibilidade Microbiana , Dados de Sequência Molecular , Peso MolecularRESUMO
In humans, adverse pregnancy outcomes (low birth weight, prematurity, and intrauterine growth retardation) are associated with exposure to urban air pollution. Experimental data have also shown that such exposure elicits adverse reproductive outcomes. We hypothesized that the effects of urban air pollution on pregnancy outcomes could be related to changes in functional morphology of the placenta. To test this, future dams were exposed during pregestational and gestational periods to filtered or nonfiltered air in exposure chambers. Placentas were collected from near-term pregnancies and prepared for microscopical examination. Fields of view on vertical uniform random tissue slices were analyzed using stereological methods. Volumes of placental compartments were estimated, and the labyrinth was analyzed further in terms of its maternal vascular spaces, fetal capillaries, trophoblast, and exchange surface areas. From these primary data, secondary quantities were derived: vessel calibers (expressed as diameters), trophoblast thickness (arithmetic mean), and total and mass-specific morphometric diffusive conductances for oxygen of the intervascular barrier. Two-way analysis of variance showed that both periods of exposure led to significantly smaller fetal weights. Pregestational exposure to nonfiltered air led to significant increases in fetal capillary surface area and in total and mass-specific conductances. However, the calibers of maternal blood spaces were reduced. Gestational exposure to nonfiltered air was associated with reduced volumes, calibers, and surface areas of maternal blood spaces and with greater fetal capillary surfaces and diffusive conductances. The findings indicate that urban air pollution affects placental functional morphology. Fetal weights are compromised despite attempts to improve diffusive transport across the placenta.
Assuntos
Material Particulado/toxicidade , Placenta/anatomia & histologia , Placenta/efeitos dos fármacos , Poluição do Ar/efeitos adversos , Animais , Animais Recém-Nascidos , Peso ao Nascer/efeitos dos fármacos , Cidades , Feminino , Tamanho da Ninhada de Vivíparos/efeitos dos fármacos , Masculino , Camundongos , Modelos Biológicos , Tamanho do Órgão/efeitos dos fármacos , Placenta/irrigação sanguínea , Placenta/ultraestrutura , GravidezRESUMO
The effects of partial urethral obstruction on the detrusor muscle of rabbit urinary bladder were investigated using stereological sampling and estimation tools. Twelve female Norfolk rabbits (2.5-3.0 kg body weight) were divided into four groups: 3, 7 and 12 weeks after surgical intervention to produce a standard partial obstruction and unobstructed controls. Following removal, bladder axes (craniocaudal, dorsoventral and laterolateral) and organ weights were recorded. Bladders were prepared for light microscopy by multistage random sampling procedures. Stereological methods were used to estimate the volume of muscle and the packing density and total number of myocyte nuclei in each bladder. We also estimated mean myocyte volume and the mean cross-sectional area and length of myocytes. Group comparisons were made by one-way analysis of variance. Changes in bladder axes were mainly laterolateral and craniocaudal. Mean bladder weight increased roughly six-fold by 3 weeks and 17-fold by 12 weeks and was accompanied, on average, by 12- and 33-fold increases in total muscle volume. These variables did not differ at 3 and 7 weeks post-obstruction. Increases in muscle content were not accompanied by changes in packing densities but were associated with increases in the total numbers of myocyte nuclei (13-fold by 3 weeks, 28-fold by 12 weeks). Mean myocyte volume did not vary significantly between groups but cells in obstructed groups were shorter and wider. These findings support the notion that partial outflow obstruction leads to an increase in the number, but not mean volume, of myocytes. If due solely to myocyte mitosis, the total of 43 x 10(8) cells found at 12 weeks could be generated by the original complement of 15 x 10(7) cells if an average of only 2.1 x 10(6) new cells was produced every hour. In reality, even this modest proliferation rate is unlikely to be achieved because myocyte proliferation rates are very low and it is possible that new myocytes can arise by differentiation of mesenchymal or other precursor cells.
Assuntos
Miócitos de Músculo Liso/patologia , Obstrução Uretral/patologia , Bexiga Urinária/patologia , Animais , Contagem de Células , Tamanho Celular , Feminino , Hiperplasia , Modelos Animais , CoelhosRESUMO
Garrotilho é uma doença infecto-contagiosa do trato respiratório superior dos eqüídeos, freqüente e importante, que causa pirexia, descarga nasal e abscedação dos linfonodos adjacentes. Acomete basicamente equídeos jovens, e é causada pelo Streptococcus equi â-hemolítico. Ocasionalmente, o garrotilho pode deixar seqüelas como sinusites, empiema das bolsas guturais e formação de abcessos à distância, principalmente no mesentério. O objetivo deste estudo é avaliar o timpanismo e empiema de bolsa gutural, clínico e cirúrgico, conseqüente a garrrotilho, ocorrido num eqüino jovem.
Assuntos
Humanos , Masculino , Feminino , Doenças Transmissíveis , Empiema , Infecções Estreptocócicas , Infecções Respiratórias , Streptococcus equiRESUMO
Objetivo: objetiva-se nesse estudo estabelecer a relaçäo entre o nível de compreensäo da prescriçäo pediátrica pela mäe ou outro acompanhante e os possíveis fatores causais da näo-adesäo ao tratamento, buscando-se, assim, melhorar a qualidade do atendimento médico.Método: foram entrevistados, aleatoriamente, cem acompanhantes de cem crianças atendidas no ambulatório de Pediatria de Hospital Universitário, no período de abril/2000 a outubro/2000, e que receberam orientaçäo e/ou receita médica na consulta. Aplicou-se um questionário que avaliou o nível socioeconômico-cultural do acompanhante, fatores relacionados à consulta e à execuçäo do tratamento e à compreensäo da caligrafia e conteúdo da "receita".Resultados: dos entrevistados, 83 por cento tinham prescriçäo e 17 por cento receberam apenas orientaçöes; 88 por cento eram mäes, e 63 por cento cursaram 1º grau incompleto. Com relaçäo à consulta, 80 por cento relataram bom nível de satisfaçäo. Daqueles com receita, 71 por cento compreenderam bem a letra da receita, e 29 por cento apresentavam compreensäo regular ou ruim. Em 47 por cento das prescriçöes, havia símbolos e/ou abreviaturas, dos quais, 59 por cento näo foram compreendidos. Quanto à compreensäo do conteúdo da receita; 71 por cento lembravam-se de todos os medicamentos prescritos; 15,7 por cento näo se lembravam, mas conseguiam ler a receita; 7,2 por cento näo se lembravam e referiam que o "farmacêutico" a explicaria; e 6 por cento näo se lembravam e nem conseguiam ler a receita.Conclusäo: conclui-se que os fatores relacionados à näo compreensäo da prescriçäo pediátrica säo o baixo nível socioeconômico-cultural do acompanhante/responsável e fatores relacionados ao médico, tais como: a utilizaçäo de símbolos/abreviaturas, letra ilegível e orientaçöes fornecidas apenas verbalmente
RESUMO
OBJECTIVE: This study intends to establish the relation between the understanding level of pediatric prescription by the mother, or other adult, accompanying the child to a doctors visit and the probable causes of noncompliance with the treatment, in order to improve the quality of pediatric health care. METHODS: We randomly interviewed one hundred adults who accompanied children to the pediatric outpatient clinic of Hospital Universitário. They received medical orientation and/or prescriptions. The interviews were made from April to October 2000 and we used a questionnaire to evaluate social, economic and cultural status. We also evaluated aspects related to the appointment and treatment, and the understanding of doctors handwriting and prescription. RESULTS: Eighty-three percent of them received a prescription and 17% received only orientation; 88% were the mothers and 63% had not finished elementary school. Concerning the appointment, 80% felt satisfied. Seventy-one percent among those who received prescriptions could understand the doctor's handwriting and 29% had some or none understanding of it. Forty-seven percent of the prescriptions had symbols and/or abbreviations that could not be understood by 59% of the adults. Seventy-one percent could remember all the prescribed drugs without looking at the prescription; 15.7% needed to read it in order to remember the drugs; 7.2% could not remember the drugs and said that a pharmacist had to help them and 6% could not remember or read the prescription. CONCLUSION: Aspects related to the poor understanding of pediatric prescriptions are caused by the low social, economic and cultural status of the adult accompanying the child; as well as aspects regarding physicians such as the use of symbols and abbreviations, illegible handwriting and verbal orientation only.
RESUMO
Os autores propoem uma técnica para artrodese do tornozelo com o uso de trefinas especialmente confeccionadas, incicadas em pacientes portadores de artroses pós-traumáticas ou com instabilidades graves. Descrevem em detalhes o instrumental empregado, o procedimento cirúrgico e efetuam a análise estatística dos 30 tornozelos operados. Concluem, que apesar de terem obtido consolidaçao óssea em 26 (87 por cento) e psuedartrose em 4 (13 por cento), sua indicaçao está limitada como artrodese in situ, estando restrita a pacientes previamente selecionados. Trata-se de método de fácil execuçao, com baixa morbidade, constituindo excelente alternativa de tratamento.
Assuntos
Humanos , Adulto , Pessoa de Meia-Idade , Artrodese , Articulação do Tornozelo/cirurgia , Pseudoartrose , Regeneração Óssea/fisiologiaRESUMO
Este trabalho tem o objetivo de apresentar as técnicas de bloqueio anestésico do pé utilizados no Setor de Medicina e Cirurgia do Pé da Universidade Federal de Säo Paulo - Escola Paulista de Medicina - Serviço do Professor Dr. José Laredo Filho - para a realizaçäo de cirurgias do mediopé e antepé em regime ambulatorial. Para tanto, foram realizados estudos em peças cadavéricas no intuito de verificar os pontos anatômicos e estabelecer os referenciais para o procedimento anestésico. Säo apresentados em detalhes os pontos anatômicos e estabalecer os referenciais para o procedimento anestésico regional, assim como a técnica utilizada e os resultados do método após ser aplicada em 50 pacientes. O método foi utilizado em pacientes criteriosamente selecionados e, apesar de ter sido uma experiência recente e restrita, podemos afirmar tratar-se de um método prático e seguro, näo tendo ocorrido nenhuma complicaçäo grave nos 50 bloqueios realizados
Assuntos
Anestesia por Condução/métodos , Pé/cirurgiaRESUMO
Os autores fazem uma análise de 46 pacientes com 69 pés afetados com o diagnóstico de metatarso varo congênito. O seguimento foi a longo prazo, com mínimo de dois anos e máximo de 19 anos e média de oito anos e nove meses. A duraçäo média de tratamento foi de três meses. Fazem uma análise dos resultados, comparando os pés no início e no fim do tratamento e mais tarde, num último exame. Utilizam a medida do ângulo entre o eixo do calcâneo e o eixo do quarto metatarsiano na radiografia ântero-posterior do pé e constatam que a dificuldade do tratamento näo dependeu da intensidade da deformidade
Assuntos
Humanos , Masculino , Feminino , Recém-Nascido , Lactente , Deformidades Congênitas do Pé/terapia , Ossos do Metatarso/anormalidades , Deformidades Congênitas do Pé , Fixação de Fratura/métodos , Seguimentos , Ossos do Metatarso , Estudos RetrospectivosRESUMO
Os autores revisam a literatura no campo das artrogriposes e ressaltam a classificaçäo proposta por Hall e col. que identifica os tipos clínicos bem definidos dessa entidade. A EMG e o exame histoquímico para a identificaçäo das patologias de base parecem ter apenas valor acadêmico, resumindo-se o tratamento às expressöes clínicas de cada paciente. Os autores relatam os resultados em 38 pacientes classificados por esse método, propondo a adoçäo dessa classificaçäo nos serviços de ortopedia
Assuntos
Humanos , Masculino , Feminino , Recém-Nascido , Lactente , Pré-Escolar , Criança , Adolescente , Adulto , Artrogripose/classificaçãoRESUMO
Os autores apresentam um caso de displasia congênita da tíbia, caracterizada por molde cartilagíneo em praticamente toda diáfise e epífise distal. Discutem a dificuldade diagnóstica desta condiçäo ao exame radiográfico e pneumoartrográfico e o erro de conduta que disto pode resultar. Apresentam o estudo histológico e tomográfico computadorizado da peça anatômica obtida na desarticulaçäo do membro ao nível do joelho e comparam os achados com os encontrados em paciente adulto näo tratado por este método