RESUMO
Various plant species can be selected for environmental testing, including pearl millet (Pennisetum glaucum (L.) R. Br), a globally significant cereal crop. This study aims to assess millet's suitability as a species for ecotoxicological tests, examining (1) germination and initial development dynamics, (2) the minimum seed quantity for reliable sampling, (3) optimal experimental design with replication numbers, (4) suitability of positive control, and (5) the effectiveness of the protocol in evaluating toxic effects of environmental pollutants. Millet exhibited rapid and uniform germination as well as consistent initial seedling development. To establish the minimum number of seeds required for reliable experimentation, germination, and seedling growth were compared across plots containing 10, 25, and 50 seeds. Consequently, 10 seeds per plot were chosen for subsequent experiments to reduce labor and costs while maintaining reliability. To validate the selected experimental design, and to establish a positive control for assays, aluminum was used as a toxic element at concentrations ranging from 10-2 to 10-6 M. While aluminum did not affect the final percentage of germinated seeds, it did exhibit an impact on the Germination Speed Index (GSI). Significant differences in root and aerial growth, and with fresh weight, were observed. The 10-3M concentration was chosen as the positive control as the 10-2 concentration showed extreme toxicity. To assess the applicability of the established protocol in determining the toxic effects of environmental pollutants, millet roots were exposed to the toxic agents atrazine, cadmium, methyl methane sulfonate (MMS), and Spent pot liner (SPL). Millet demonstrated sensitivity and efficiency in response to these tests. In conclusion, millet proves to be an effective species for the toxicological risk assessment of environmental pollutants.
Assuntos
Ecotoxicologia , Germinação , Pennisetum , Pennisetum/efeitos dos fármacos , Germinação/efeitos dos fármacos , Plântula/efeitos dos fármacos , Plântula/crescimento & desenvolvimentoRESUMO
Pollution generated by deposition of industrial activity waste in the environment without due care can lead to serious environmental consequences. Bioassays in higher plants are means of understanding the cytogenotoxic effects of these substances. In the present work, Allium cepa L. was used as a model species to assess nucleolar changes induced by environmental pollutants. The substances used were Methyl Methane Sulfonate (MMS), cadmium (Cd), Spent Potliner (SPL) and the herbicide Atrazine. Water was used as a negative control. The silver-stained nucleolar organizer region (AgNOR) assay was used making it possible to evaluate how nucleolar parameters (number of nucleoli per nucleus and nucleoli area) behave when facing stress caused by such pollutants. The results obtained showed a variation in the observed parameters: an increase in the number of nucleoli in the treated cells and tendency to a reduction in nucleolar area, indicating that the tested pollutants may have impaired nucleolar activity. In addition, it was possible to establish a relationship between the behavior of the nucleolus with other changes as plantlet growth, cell proliferation, and DNA damage.
Assuntos
Nucléolo Celular/efeitos dos fármacos , Monitoramento Ambiental/métodos , Poluentes Ambientais/toxicidade , Resíduos Industriais/efeitos adversos , Mutagênicos/toxicidade , Nucléolo Celular/patologia , Núcleo Celular/efeitos dos fármacos , Núcleo Celular/patologia , Biomarcadores Ambientais/efeitos dos fármacos , Cebolas/citologia , Cebolas/efeitos dos fármacosRESUMO
Studies that help understand the mechanisms of action of environmental pollutants are extremely important in environmental toxicology. In this context, assays using plants as models stand out for their simplicity and low performance cost. Among the plants used for this purpose, Allium cepa L. is the model most commonly applied for cytogenotoxic tests, while Lactuca sativa L., already widely used in phytotoxic investigations, has been gaining prominence in cytotoxic analyses. The present study aimed to compare the responses of A. cepa and L. sativa via macroscopic (root growth) and microscopic analyses (cell cycle and DNA fragmentation via TdT-mediated deoxy-uracil nick and labeling (TUNEL) and comet assays) after exposure of their roots to environmental pollutants with known cytogenotoxic mechanisms. Both species presented sensitive and efficient response to the applied tests after exposure to the DNA-alkylating agent Methyl Methanesulfonate (MMS), the heavy metal Cadmium, the aluminum industry waste Spent Potliner (SPL) and the herbicide Atrazine. However, they differed regarding the responses to the evaluated endpoints. Overall, A. cepa was more efficient in detecting clastogenic changes, arising from DNA breakage, while L. sativa rather detected aneugenic alterations, related to chromosome segregation in mitosis. In the tests applied to verify DNA fragmentation (comet and TUNEL assays), A. cepa presented higher sensitivity. In conclusion, both models are efficient to evaluate toxicological risks of environmental pollutants.