RESUMO
This article reports the experience of the design team of the Laboratory The Imaginary in the process of developing an electric potter's lathe which respects the biomechanics of the body, helps to development the task, maintains the archetype of traditional equipment, and improves the mechanical efficiency of the power transmission system. The design method used was based on the axes of research, analysis, development and monitoring, and focused on the product and with partners: the artisans and engineering and production teams. The main results point to ergonomic improvements in the biomechanical and dimensional aspects, and a decrease in the risk of accidents and occupational diseases. The experience of this case also highlights the gains arising from the relationship between design, engineering and users (artisans) in developing products with a design that can be easily replicated for other communities of potters in the state. This and other actions are part of the outlook of the Laboratory the Imaginary which in partnership with local authorities, wishes to see the craft activities continue and to give value to the culture of the city.
Assuntos
Cerâmica , Desenho de Equipamento , Ergonomia , Fenômenos Biomecânicos , Brasil , Eficiência , Fontes de Energia Elétrica , Humanos , Doenças Musculoesqueléticas/prevenção & controle , Doenças Profissionais/prevenção & controleRESUMO
Renin is an enzyme involved in the stepwise generation of angiotensin II. Juxtaglomerular cells are the main source of plasma renin, but renin activity has been detected in other cell types. In the present study we evaluated the presence of renin mRNA in adult male Wistar rat and mouse (C-57 Black/6) mesangial cells (MC) and their ability to process, store and release both the active and inactive forms of the enzyme. Active renin and total renin content obtained after trypsin treatment were estimated by angiotensinogen consumption analyzed by SDS-PAGE electrophoresis and quantified by angiotensin I generation by HPLC. Renin mRNA, detected by RT-PCR, was present in both rat and mouse MC under basal conditions. Active renin was significantly higher (P<0.05) in the cell lysate (43.5 +/- 5.7 ng h-1 10(6) cells) than in the culture medium (12.5 +/- 2.5 ng h-1 10(6) cells). Inactive prorenin content was similar for the intra- and extracellular compartments (9.7 +/- 3.1 and 3.9 +/- 0.9 ng h-1 10(6) cells). Free active renin was the predominant form found in both cell compartments. These results indicate that MC in culture are able to synthesize and translate renin mRNA probably as inactive prorenin which is mostly processed to active renin inside the cell. MC secrete both forms of the enzyme but at a lower level compared with intracellular content, suggesting that the main role of renin synthesized by MC may be the intracellular generation of angiotensin II
Assuntos
Animais , Masculino , Camundongos , Ratos , Mesângio Glomerular , Renina , Cromatografia Líquida de Alta Pressão , Eletroforese em Gel de Poliacrilamida , Ativação Enzimática , Mesângio Glomerular , Técnica de Amplificação ao Acaso de DNA Polimórfico , Ratos Wistar , Renina , Reação em Cadeia da Polimerase Via Transcriptase Reversa , RNA MensageiroRESUMO
Renin is an enzyme involved in the stepwise generation of angiotensin II. Juxtaglomerular cells are the main source of plasma renin, but renin activity has been detected in other cell types. In the present study we evaluated the presence of renin mRNA in adult male Wistar rat and mouse (C-57 Black/6) mesangial cells (MC) and their ability to process, store and release both the active and inactive forms of the enzyme. Active renin and total renin content obtained after trypsin treatment were estimated by angiotensinogen consumption analyzed by SDS-PAGE electrophoresis and quantified by angiotensin I generation by HPLC. Renin mRNA, detected by RT-PCR, was present in both rat and mouse MC under basal conditions. Active renin was significantly higher (P<0.05) in the cell lysate (43.5 +/- 5.7 ng h-1 10(6) cells) than in the culture medium (12.5 +/- 2.5 ng h-1 10(6) cells). Inactive prorenin content was similar for the intra- and extracellular compartments (9.7 +/- 3.1 and 3.9 +/- 0.9 ng h-1 10(6) cells). Free active renin was the predominant form found in both cell compartments. These results indicate that MC in culture are able to synthesize and translate renin mRNA probably as inactive prorenin which is mostly processed to active renin inside the cell. MC secrete both forms of the enzyme but at a lower level compared with intracellular content, suggesting that the main role of renin synthesized by MC may be the intracellular generation of angiotensin II.