RESUMO
The relationships between temporomandibular joint (TMJ) disorders and cervical structure dysfunctions have already been demonstrated. The aim of the present study was to investigate functional and structural alterations of the head and neck of 17 individuals with TMJ disorders (TMD group), compared with a control group of 17 asymptomatic subjects in a cross-sectional design. The outcome variables included pain on palpation of the sternocleidomastoideus, superior trapezius and subocciptal muscles, as well as radiographic measures of alignment of the cervical spine and positioning of the hyoid bone. Cervical alignment, determined by measures of the cervical curvature angle, was investigated by the Cobb method. Independent Student t-tests were used to investigate differences between groups for all outcome variables (alpha < 0.05). The results demonstrated that individuals with TMJ disorders, when compared with asymptomatic subjects, presented higher levels of perception of pain in all cervical muscles (P < 0.0001). No significant differences were found between groups for the cervical alignment measures. In the TMD group, the position of the hyoid bone in relation to the cervical spine did not appear to be different from the control group.
Assuntos
Vértebras Cervicais/fisiopatologia , Osso Hioide/fisiopatologia , Transtornos da Articulação Temporomandibular/fisiopatologia , Adulto , Índice de Massa Corporal , Vértebras Cervicais/diagnóstico por imagem , Vértebras Cervicais/patologia , Estudos Transversais , Feminino , Humanos , Osso Hioide/diagnóstico por imagem , Osso Hioide/patologia , Hiperalgesia/etiologia , Masculino , Músculos do Pescoço/fisiopatologia , Palpação , Radiografia , Transtornos da Articulação Temporomandibular/complicações , Transtornos da Articulação Temporomandibular/diagnóstico por imagem , Transtornos da Articulação Temporomandibular/patologiaRESUMO
Thermostable invertase (E.C. 3.2.1.26) and inulinase 2,1-beta-D-fructan fructanohydrolase (E.C. 3.2.1.7) activities were produced by Cladosporium cladosporioides grown on sucrose, inulin, yam extract, or Jerusalem artichoke. The ratio I (inulinase)/S(invertase) activity was between 0.31 and 0.36. Both activities had high temperature optima (60 degrees C) and were stable during pretreatment for 4.5 h at this temperature. Whole cells of C. cladosporioides were used for batch fructose production from Jerusalem artichoke extract at several concentrations. With the highest extract concentration used (260 g total sugars/L), total hydrolysis was achieved in 150 min at 60 degrees C. Thin-layer chromatography of the enzymatic hydrolysis of inulin and Jerusalem artichoke extract showed that from the beginning of the reaction, fructose was the only product released. This suggests an exoaction mechanism, beta-D-fructofuranoside fructohydrolase [E.C. 3.2.1.2.6].