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1.
Int J Occup Saf Ergon ; : 1-12, 2024 Aug 06.
Artigo em Inglês | MEDLINE | ID: mdl-39108143

RESUMO

Objectives. Work-related musculoskeletal disorders (WMSDs) are recurrent in the footwear industry, resulting in absenteeism. This study aimed to quantitatively analyze the influence of overtime work and physical recovery time on the occurrence of WMSD-related absenteeism using a system dynamics model. As ergonomic methods have limitations in quantitatively simulating the behavior of these relationships, the integration of computational modeling techniques has emerged as a methodological alternative to bridge this gap. Methods. An ergonomic work analysis (EWA) was developed in a production cell of a large company. A model of causal relationships (causal loop diagram) and a simulation model (flow and stock diagram) were then developed, where three scenarios for overtime and physical recovery time were analyzed. Results. Working an additional hour resulted in a 42% increase in physical overload, leading to 7.62 leave requests per year and 78.7 days of employee absenteeism. Increasing the physical recovery time by 15 min reduced the overload to 36.5%, resulting in 6.8 leave requests per year and 71.1 days of employee absenteeism. Conclusions. Properly managing excess workload and providing adequate physical recovery for professionals is necessary to mitigate the productivity impacts of absenteeism in the footwear industry.

2.
Ann Clin Microbiol Antimicrob ; 22(1): 64, 2023 Aug 02.
Artigo em Inglês | MEDLINE | ID: mdl-37533063

RESUMO

PURPOSE: The aim of this study was to assess the risk factors for colistin-resistant carbapenemase-producing Enterobacterales (CR-CPE), and describe the mortality associated with this organism, in a low-income country. METHODS: A descriptive, observational, and prospective multicenter study was carried out in Guayaquil, Ecuador. All patients with carbapenem-resistant Enterobacterales admitted between December 2021 and May 2022 were enrolled. Infection definitions were established according to the Centers for Disease Control and Prevention (CDC) protocols. The presence of carbapenemase-producing Enterobacterales was confirmed with a multiplex PCR for blaKPC, blaNDM, blaOXA-48, blaVIM, and blaIMP genes. MCR-1 production was studied molecularly, and MLST assays were carried out. RESULTS: Out of 114 patients enrolled in the study, 32 (28.07%) had at least one positive sample for CR-CPE. Klebsiella pneumoniae ST512-KPC-3 was the most frequent microorganism isolated. Parenteral feeding, ß-lactamase inhibitor use, recent hemodialysis, and renal failure were all considered independent risk factors for carrying CR-CPE. A mortality of 41.22% was detected, but we could not find any difference between colistin-resistant and colistin-susceptible CPE. MCR-1 production was not detected in any of the isolates studied. CONCLUSION: A significant burden for CR-CPE was found in a South American country that was mainly caused by the high-risk clone K. pneumoniae ST512-KPC-3 and not mediated by mcr-1 production. Its acquisition involved parenteral feeding, ß-lactamase inhibitor use, recent hemodialysis, and renal failure as independent risk factors, demonstrating the critical need for infection prevention and stewardship programs to avoid dissemination to other countries in the region.


Assuntos
Colistina , Inibidores de beta-Lactamases , Humanos , Colistina/farmacologia , Tipagem de Sequências Multilocus , Estudos Prospectivos , Proteínas de Bactérias/genética , beta-Lactamases/genética , Klebsiella pneumoniae , Monobactamas , Fatores de Risco , Testes de Sensibilidade Microbiana , Antibacterianos/farmacologia
3.
Arch Psychiatr Nurs ; 45: 54-60, 2023 08.
Artigo em Inglês | MEDLINE | ID: mdl-37544702

RESUMO

BACKGROUND: The occupational activities carried out in hospital environments pose occupational risks to professionals. In psychiatric hospitals, due to the characteristics of the patients treated, professionals are also subjected to other risks, such as physical aggression. OBJECTIVE: This research aimed to identify the systemic context, highlighting the cause-and-effect relationships that culminate in occupational accidents that occurred with the nursing staff in a psychiatric hospital in Brazil. METHODS: The current study is an applied research and was divided into three stages. First, the collection of data related to the case study was made and accidents were analyzed and occupational hazards were identified. In the second stage, from the collected information, occupational safety indicators were defined. Lastly, in the third stage, the qualitative aspect of System Dynamics was applied to perform the systemic analysis and to identify how the different variables were related. RESULTS: The results showed that physical aggression was the main cause of accidents. Regarding safety indicators, while both the level of use of Personal Protective Equipment (PPE) by professionals and the high level of PPE protection were positive aspects, the level of training of professionals to use PPE was a negative aspect. The Causal Link Diagram (CLD) showed that the perception of risk influenced the level of use of PPE and those organizational measures influenced the accident rate. CONCLUSION: In conclusion, the systemic analysis of the system dynamics can optimize the diagnostic process of occupational accidents in psychiatric hospitals, and especially help to identify the cause and effect among the variables involved.


Assuntos
Recursos Humanos de Enfermagem , Saúde Ocupacional , Humanos , Acidentes de Trabalho/prevenção & controle , Hospitais Psiquiátricos , Brasil
4.
Biotechnol Prog ; 37(2): e3101, 2021 03.
Artigo em Inglês | MEDLINE | ID: mdl-33169497

RESUMO

Biotechnology advances have allowed bacteria, yeasts, plants, mammalian and insect cells to function as heterologous protein expression systems. Recently, microalgae have gained attention as an innovative platform for recombinant protein production, due to low culture media cost, compared to traditional systems, as well as the fact that microalgae such as Chlamydomonas reinhardtii are considered safe (GRAS) by the Food and Drug Administration (FDA). Previous studies showed that recombinant protein production in traditional platforms by semicontinuous process increased biomass and bio product productivity, when compared to batch process. As there is a lack of studies on semicontinuous process for recombinant protein production in microalgae, the production of recombinant mCherry fluorescent protein was evaluated by semicontinuous cultivation of Chlamydomonas reinhardtii in bubble column photobioreactor. This semicontinuous cultivation process was evaluated in the following conditions: 20%, 40%, and 60% culture portion withdrawal. The highest culture withdrawal percentage (60%) provided the best results, as an up to 161% increase in mCherry productivity (454.5 RFU h-1 - Relative Fluorescence Unit h-1 ), in comparison to batch cultivation (174.0 RFU h-1 ) of the same strain. All cultivations were carried out for 13 days, at pH 7, temperature 25°C and, by semicontinuous process, two culture withdrawals were taken during the cultivations. Throughout the production cycles, it was possible to obtain biomass concentration up to 1.36 g L-1 .


Assuntos
Técnicas de Cultura de Células/métodos , Chlamydomonas reinhardtii/metabolismo , Meios de Cultura/metabolismo , Substâncias Luminescentes/metabolismo , Proteínas Luminescentes/biossíntese , Fotobiorreatores/normas , Proteínas Recombinantes/biossíntese , Biomassa , Chlamydomonas reinhardtii/genética , Chlamydomonas reinhardtii/crescimento & desenvolvimento , Proteínas Luminescentes/genética , Proteínas Luminescentes/metabolismo , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Proteína Vermelha Fluorescente
5.
Cad. Bras. Ter. Ocup ; 28(4): 1297-1310, Oct.-Dec. 2020. graf
Artigo em Português | LILACS-Express | LILACS, Index Psicologia - Periódicos | ID: biblio-1153632

RESUMO

Resumo Introdução A Tecnologia Assistiva (TA) é apontada como uma das soluções para proporcionar uma melhor funcionalidade e qualidade de vida às pessoas com doenças reumatológicas. Projetos interdisciplinares com foco em avaliar, indicar e desenvolver TA são importantes para os avanços na pesquisa e assistência clínica. A captura de movimento, por meio da instrumentação tecnológica, apresenta-se como um tema inovador por fornecer dados objetivos sobre o usuário. Mais estudos nessa área são necessários para conhecer as possibilidades de aplicação da instrumentação tecnológica em pesquisas de TA. Objetivo Descrever a aplicabilidade da captura de movimento, por meio de um equipamento de alta tecnologia, numa pesquisa interdisciplinar de TA para pacientes reumatológicos. Método Trata-se de Relato de Experiência, com uma abordagem qualitativa, no qual foi descrito as ações utilizando um equipamento tecnológico específico para captura de movimentos. Resultados Foi utilizada a captura de movimento com três finalidades: apoio educacional; avaliação de usabilidade de recursos de TA desenvolvidos; e suporte a grupos de estudo de terapeutas ocupacionais para análises de atividades. Conclusão Nas diferentes ações, a captura de movimento funcionou como um recurso complementar para as análises de atividades realizadas pelos terapeutas ocupacionais, o que contribuiu para a indicação, desenvolvimento e avaliação dos dispositivos de TA e para facilitar as orientações dadas no manual e nos grupos de orientação sobre Proteção Articular. O trabalho interdisciplinar foi o diferencial para o uso adequado do equipamento.


Abstract Introduction Assistive Technology (AT) is pointed as one of the possible solutions to offer better functionality and quality of life for people with rheumatological diseases. Interdisciplinary projects focused on assessing, indicating, and developing AT are important for advances in research and clinical care. Motion capture, through technological instrumentation, is an innovative theme for providing objective data about the user. Further studies in this area are needed to know the possibilities of applying technological instrumentation in AT research. Objective To describe the applicability of motion capture, using high-tech equipment, in an interdisciplinary AT research for rheumatological patients. Method It is an Experience Report, with a qualitative approach, in which the actions were described using specific technological equipment to motion capture. Results: Motion capture was used for three purposes: educational support; usability evaluation of AT resources developed; and support for study groups of occupational therapists to activity analysis. Conclusion In the different actions, the motion capture was used as a complementary resource for activities' analysis performed by occupational therapists, which contributed to the indication, development, and evaluation of AT devices and to facilitate the instructions provided in manual and in the guidance groups about Joint Protection. Interdisciplinary work was differentiated for the proper use of equipment.

6.
Rev. colomb. cardiol ; 26(6): 342-345, nov.-dic. 2019. graf
Artigo em Espanhol | LILACS, COLNAL | ID: biblio-1115591

RESUMO

Resumen El derrame pericárdico es una acumulación excesiva de líquido en el espacio pericárdico, el cual se ha asociado al empleo crónico de minoxidil desde principios de su uso clínico; este es formulado comúnmente a pacientes con hipertensión arterial de difícil control y con enfermedad renal crónica asociada, por su efecto vasodilatador arterial y poco efecto sobre la circulación venosa. Se expone el caso de un paciente quien presentó clínica sugestiva de derrame pericárdico, el cual fue confirmado por imágenes (rayos X, tomografía y ecocardiografía) y quien además se encontraba en tratamiento de hemodiálisis crónica por enfermedad renal crónica secundaria a síndrome nefrótico. En la literatura existen algunos reportes de casos similares, pero no hay estudios con datos concluyentes que permitan establecer un porcentaje claro de asociación ni la causa de esta enfermedad. Con este reporte de caso se busca aumentar la sospecha diagnóstica de esta asociación para que otros clínicos tengan este posible diagnóstico en mente, una vez se hayan descartado otras etiologías adicionales y puedan suspender a tiempo la medicación a fin de evitar desenlaces catastróficos como el taponamiento pericárdico.


Abstract Pericardial effusion is an excessive accumulation of fluid in the pericardial space and has been associated with the long-term use of minoxidil from the beginning of its clinical use. It is commonly prescribed to patients with difficult to control arterial hypertension and associated chronic kidney disease due to it arterial vasodilator effects and little effect of the venous circulation. A case is presented on a patient who had a clinical picture suggestive of a pericardial effusion, which was confirmed by imaging tests (X-ray, tomography, and cardiac ultrasound). She was also on long-term haemodialysis treatment due to chronic kidney disease secondary to a nephrotic syndrome. There are reports of similar cases in the literature, but there are no studies with conclusive data that may help to establish a clear percentage association or the cause of the disease. This case report seeks to increase the diagnostic suspicion of this association so that other clinicians may have this possible diagnosis in mind, once they have ruled out any other. They can then stop the medication on time in order to prevent catastrophic outcomes like pericardiac tamponade.


Assuntos
Humanos , Masculino , Adulto , Derrame Pericárdico , Ecocardiografia , Tamponamento Cardíaco , Raios X , Tomografia , Insuficiência Renal Crônica , Hipertensão , Minoxidil
7.
J Contam Hydrol ; 227: 103542, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31471099

RESUMO

In this study, silica and functionalized silica materials (3-aminopropyl and 3-mercapto derivatives) were successfully used for the removal of the pesticides bentazone and imazapyc from aqueous solutions. Adsorbent materials were characterized by BET isotherms and FT-IR spectroscopy (confirming the functionalization), and their equilibrium adsorption capacity was evaluated at different ionic strengths. It is observed that the maximum adsorption capacities decrease in the order 3-aminopropyl-derivative > silica >3-mercaptopropyl derivative. An increase in ionic strength produces an enhancement in the removal of pesticides. All isotherms are Ib-type and follow the Langmuir model, suggesting a monolayer physical adsorption process.


Assuntos
Dióxido de Silício , Poluentes Químicos da Água , Adsorção , Benzotiadiazinas , Cinética , Espectroscopia de Infravermelho com Transformada de Fourier , Água
8.
J. health sci. (Londrina) ; 21(1)30/04/2019.
Artigo em Inglês | LILACS | ID: biblio-995913

RESUMO

The cultivation of marine mollusks is an activity of great importance in Brazil, providing employment and income for artisanal fishermen and fishing communities, contributing to local development. However, this type of cultivation is still carried out in a very handmade way with intense use of labor. The objective of this study was to describe the postures and movements of fish farmers during the oyster classification activity. Ten employees of a marine farm participated in the municipality of Florianópolis, SC. For the analysis of the posture and movements, the Rapid Entire Body Assessment (REBA) method and NIOSH method were used along with in situ observation and interviews with the workers. The task of oysters classification presents an average risk of injury or occupational diseases, especially in the cervical region and upper limbs, as it is developed with marked flexion of the head and shoulders, which requires a lot of the muscles involved to maintain the posture and perform repetitive movements. Many workers have muscle pains in the shoulders, lower back, hands, wrists and fingers. Simple and economically viable adaptations should be adopted, because through ergonomic evaluation it is possible to carry out interventions that are within the reach of the workers. It is necessary to transmit knowledge so that the workers are aware of the postures that they must adopt in the accomplishment of this aquaculture activity and thus to promote a better quality of life of this population. (AU).


O cultivo de moluscos marinhos é uma atividade de grande importância no Brasil proporcionando a geração de emprego e renda para pescadores artesanais e comunidades pesqueiras, contribuindo para o desenvolvimento local. Entretanto, este tipo de cultivo ainda é realizado de forma bastante artesanal com intensa utilização de mão de obra. Diante disso, este estudo teve por objetivo realizar uma descrição das posturas e movimentos de maricultores durante a realização da atividade de classificação de ostras. Participaram voluntariamente dez funcionários de uma fazenda marinha no município de Florianópolis, SC. Para análise da postura e movimentos, utilizou-se o método Rapid Entire Body Assessment (REBA) e método NIOSH juntamente com a observação in loco e entrevistas com os maricultores. A tarefa de classificação de ostras apresenta médio risco de lesão ou doenças ocupacionais, principalmente na região cervical e membros superiores, pois é desenvolvida com acentuada flexão de cabeça e ombros, o que exige muito da musculatura envolvida para manter a postura e realizar movimentos repetitivos. Muitos trabalhadores apresentam dores musculares nos ombros, lombar, mãos, punhos e dedos. Adaptações simples e economicamente viáveis devem ser adotadas, pois através da avaliação ergonômica é possível realizar intervenções que estão ao alcance dos trabalhadores. Necessitase transmitir os conhecimentos para que os trabalhadores tenham consciência das posturas que devem adotar na realização desta atividade aquícola e assim promover uma melhor qualidade de vida desta população. (AU).

9.
Appl Ergon ; 77: 1-8, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30832772

RESUMO

The purpose of this study is to construct an aircraft seat discomfort scale and verify the evidence of its validity and reliability. We developed a questionnaire and presented it to 1500 Brazilian passengers at an airport. The data analysis included: 1) factor analysis; 2) the analysis of the items to be used in the scale created using Item Response Theory. The scale covered all levels, from "without discomfort" to "maximum discomfort". At the level of minimal, a passenger feels that the aircraft seat is cramped and has little space. Discomfort tends to increase when passengers are unable to perform desired activities (eg: reading, sleeping), which causes irritation and disappointment. The maximum discomfort level occurs when noise disrupts activities and an aircraft seat is considered hard. The scale developed presented indications that it is reliable and valid, proving to be a useful tool for identifying levels of discomfort in aircraft seats.


Assuntos
Aeronaves , Comportamento do Consumidor/estatística & dados numéricos , Ergonomia/métodos , Decoração de Interiores e Mobiliário , Inquéritos e Questionários/normas , Adulto , Brasil , Análise Fatorial , Feminino , Humanos , Masculino , Psicometria , Reprodutibilidade dos Testes
10.
Appl Ergon ; 79: 107-121, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-30121119

RESUMO

Although the workstations of a Brazilian automotive electrical harness production line are set close to TAKT time (the production rate required to meet demand), factory performance is compromised regarding: (i) sick leaves due to occupational disease (105 employees last year) and (ii) a production rate at only 42% of capacity. Our objective was to simulate the performance of a production line balanced against physical overload by the addition of an extra workstation. Based on ergonomic work analysis, the study applied System Dynamics at the global observation stage to obtain a systemic interpretation of the factors involved in production line performance. According to the indicators, the alternative configuration reduced physical overload by 36%, which would result in a sick leave rate of 50.8 employees/year (51.6% lower than the current configuration), as well as a production rate at 99% of capacity (a 92.7% increase over the current configuration). We found that reducing physical overload allows the "workforce control" loop to govern the system, producing favorable results. We conclude that setting the work cycle overly close to TAKT time leads to overload, due to the shorter recovery times at the end of each cycle. Thus, it is necessary to seek a balance between efficiency gains through downtime reduction and the physiological recovery of workers.


Assuntos
Instalações Industriais e de Manufatura , Doenças Profissionais/epidemiologia , Licença Médica/estatística & dados numéricos , Desempenho Profissional , Carga de Trabalho/psicologia , Adulto , Automóveis , Brasil , Simulação por Computador , Eficiência/fisiologia , Ergonomia , Feminino , Humanos , Masculino , Doenças Profissionais/etiologia
11.
J Contam Hydrol ; 218: 84-93, 2018 11.
Artigo em Inglês | MEDLINE | ID: mdl-30342836

RESUMO

In this study, commercial activated carbons (GAB and CBP) were successfully used for the removal of two phenoxy acetic class-herbicides, 4-chloro-2-methyl phenoxy acetic acid and 2.4-dichlorophenoxy acetic acid (MCPA and 2.4-D) from aqueous solution. The adsorbent materials were characterized, and their equilibrium adsorption capacity was evaluated. The results suggest that the microporous properties of GAB activated carbon enhanced the adsorption capacity, in comparison to CBP carbon. Thus, the increasing in the ionic strength favored the adsorption removal of both pesticides, indicating that electrostatic interactions between the pollutant and the adsorbate surface are governing the adsorption mechanism, but increasing pH values decreased adsorption capacity. Experimental data for equilibrium was analyzed by two models: Langmuir and Freundlich. Finally, computational simulation studies were used to explore both the geometry and energy of the pesticides adsorption.


Assuntos
Carvão Vegetal , Herbicidas , Poluentes Químicos da Água , Purificação da Água , Adsorção , Carbono/química , Carvão Vegetal/química , Herbicidas/química , Concentração de Íons de Hidrogênio , Cinética , Concentração Osmolar , Praguicidas , Água , Poluentes Químicos da Água/química , Purificação da Água/métodos
12.
Acta Paul. Enferm. (Online) ; 30(5): 546-553, Set.-Out. 2017. tab, graf
Artigo em Português | BDENF - Enfermagem, LILACS | ID: biblio-885875

RESUMO

Resumo Objetivo: Investigar na literatura o conhecimento disponível sobre a interdependência dos agentes desencadeantes do absenteísmo da enfermagem hospitalar para inferir, a partir de uma perspectiva multicausal, possíveis ações de gestão e controle. Métodos: Revisão sistemática compreendendo o recorte temporal de 2013 à 2017, utilizando descritores operacionalizados em bases de dados indexadas do portal da Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). A busca seguiu a metodologia Statement Preferred Reporting items For Systematic Reviews And Meta-Analyses (PRISMA) auxiliada pelo software EndNote®. Resultados: Finalizado o processo de depuração dos 269 artigos recuperados, 39 atenderam aos critérios de inclusão estabelecidos e, deles, 23,08% atribuiu a origem do absenteísmo a causas diversas não específicas. A organização do trabalho foi identificada como causa em 20,51% do material analisado, os adoecimentos musculoesqueléticos 15,38% e, os transtornos mentais e comportamentais totalizaram 10,26%. A síndrome de Burnout, 7,69% dos artigos, a satisfação no trabalho e os aspectos psicossociais 5,13% cada, relacionamento com demais membros das equipes, assédio e resiliência, ambiente laboral, fadiga e conflitos com o paciente, cada um, com 2,56% dos artigos analisados na revisão sistemática. Conclusão: Pelos resultados obtidos, concluiu-se que o absenteísmo deve ser tratado a partir de uma perspectiva múltipla, holística, epidemiológica e prospectiva através de variáveis organizacionais, físicas e cognitivas compatíveis com as análises multicausais.


Abstract Objective: To search the literature for information on interdependent factors influencing absenteeism of hospital nurses using a multicausal perspective analysis and to identify possible management and control actions. Methods: This systematic review included publications from 2013 to 2017. We used keywords from databases available at the portal of Coordination for the Improvement of Higher Education Personnel (CAPES). EndNote® software was used to manage data, and we followed the PRISMA (Preferred Reporting Items For Systematic Reviews and Meta-Analyses (PRISMA) method. Results: A total of 269 articles were retrieved, and 39 of them met our inclusion criteria. Studies that attributed absenteeism to a number of non-specific reasons accounted for 23.08% of the sample. The organization in work environment was defined as the reason in 20.51% of reports, musculoskeletal disease was the main reasons in 7.69%, and mental and behavioral disorders made up 10.26% of pointed causes. The Burnout syndrome was attributed as the reason for absenteeism in 7.69% of reports; satisfaction in work and psychological aspects accounted for 5.13% each in the studies, and relationship with other members of the nursing team, bullying and resilience, work environment, fatigue and conflicts with patients each were the reason in 2.56% of the studies analyzed in our systematic review. Conclusion: Absenteeism must be treated from a multiple, holistic, epidemiologic and prospective perspectives by adoption of organizational, physical and cognitive variables that are compatible with multicausal analyses.


Assuntos
Absenteísmo , Política de Saúde , Administração Hospitalar , Serviço Hospitalar de Enfermagem , Análise Multivariada
13.
Braz. J. Microbiol. ; 48(3): 419-426, jul.-set. 2017. tab, graf, ilus
Artigo em Inglês | VETINDEX | ID: vti-728606

RESUMO

Antibodies and antibody fragments are nowadays among the most important biotechnological products, and Pichia pastoris is one of the most important vectors to produce them as well as other recombinant proteins. The conditions to effectively cultivate a P. pastoris strain previously genetically modified to produce the single-chain variable fragment anti low density lipoprotein (-) under the control of the alcohol oxidase promoter have been investigated in this study. In particular, it was evaluated if, and eventually how, the carbon source (glucose or glycerol) used in the preculture preceding cryopreservation in 20% glycerol influences both cell and antibody fragment productions either in flasks or in bioreactor. Although in flasks the volumetric productivity of the antibody fragment secreted by cells precultured, cryopreserved and reactivated in glycerol was 42.9% higher compared with cells precultured in glucose, the use of glycerol in bioreactor led to a remarkable shortening of the lag phase, thereby increasing it by no less than thrice compared to flasks. These results are quite promising in comparison with those reported in the literature for possible future industrial applications of this cultivation, taking into account that the overall process time was reduced by around 8h.(AU)


Assuntos
Enzimas , Anticorpos de Cadeia Única , Criopreservação , Saccharomycetales/genética , Biofarmácia , Glicerol
14.
Braz. j. microbiol ; Braz. j. microbiol;48(3): 419-426, July-Sept. 2017. tab, graf
Artigo em Inglês | LILACS | ID: biblio-889135

RESUMO

Abstract Antibodies and antibody fragments are nowadays among the most important biotechnological products, and Pichia pastoris is one of the most important vectors to produce them as well as other recombinant proteins. The conditions to effectively cultivate a P. pastoris strain previously genetically modified to produce the single-chain variable fragment anti low density lipoprotein (-) under the control of the alcohol oxidase promoter have been investigated in this study. In particular, it was evaluated if, and eventually how, the carbon source (glucose or glycerol) used in the preculture preceding cryopreservation in 20% glycerol influences both cell and antibody fragment productions either in flasks or in bioreactor. Although in flasks the volumetric productivity of the antibody fragment secreted by cells precultured, cryopreserved and reactivated in glycerol was 42.9% higher compared with cells precultured in glucose, the use of glycerol in bioreactor led to a remarkable shortening of the lag phase, thereby increasing it by no less than thrice compared to flasks. These results are quite promising in comparison with those reported in the literature for possible future industrial applications of this cultivation, taking into account that the overall process time was reduced by around 8 h.


Assuntos
Pichia/metabolismo , Microbiologia Industrial/métodos , Carbono/metabolismo , Anticorpos de Cadeia Única/biossíntese , Anticorpos/metabolismo , Pichia/crescimento & desenvolvimento , Pichia/genética , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Meios de Cultura/metabolismo , Meios de Cultura/química , Anticorpos de Cadeia Única/genética , Fermentação , Glicerol/metabolismo , Lipoproteínas LDL/imunologia , Anticorpos/genética
15.
Appl Ergon ; 62: 216-226, 2017 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-28411732

RESUMO

This article aims to evaluate the psychometric properties of a scale that measures aircraft seat comfort. Factor analysis was used to study data variances. Psychometric quality was checked by using Item Response Theory. The sample consisted of 1500 passengers who completed a questionnaire at a Brazilian airport. Full information factor analysis showed the presence of one dominant factor explaining 34% of data variance. The scale generated covered all levels of comfort data, from 'no comfort' to 'maximum comfort'. The results show that the passengers consider there is comfort, but this is very minimal when these passengers have to perform their desired activities. It tends to increase when aspects of the aircraft seating are improved and positive emotions are elicited. Comfort peaks when pleasure is experienced and passenger expectations are exceeded (maximum comfort). This outcome seems consistent with the literature. Further research is advised to compare the outcome of this questionnaire with other research methods, and to check if the questionnaire is sensitive enough and whether its conclusions are useful in practice.


Assuntos
Aeronaves/instrumentação , Decoração de Interiores e Mobiliário , Inquéritos e Questionários , Adulto , Brasil , Comportamento do Consumidor , Ergonomia , Análise Fatorial , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Ruído dos Transportes , Percepção , Prazer , Psicometria , Segurança
16.
Braz J Microbiol ; 48(3): 419-426, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28237678

RESUMO

Antibodies and antibody fragments are nowadays among the most important biotechnological products, and Pichia pastoris is one of the most important vectors to produce them as well as other recombinant proteins. The conditions to effectively cultivate a P. pastoris strain previously genetically modified to produce the single-chain variable fragment anti low density lipoprotein (-) under the control of the alcohol oxidase promoter have been investigated in this study. In particular, it was evaluated if, and eventually how, the carbon source (glucose or glycerol) used in the preculture preceding cryopreservation in 20% glycerol influences both cell and antibody fragment productions either in flasks or in bioreactor. Although in flasks the volumetric productivity of the antibody fragment secreted by cells precultured, cryopreserved and reactivated in glycerol was 42.9% higher compared with cells precultured in glucose, the use of glycerol in bioreactor led to a remarkable shortening of the lag phase, thereby increasing it by no less than thrice compared to flasks. These results are quite promising in comparison with those reported in the literature for possible future industrial applications of this cultivation, taking into account that the overall process time was reduced by around 8h.


Assuntos
Anticorpos/metabolismo , Carbono/metabolismo , Microbiologia Industrial/métodos , Pichia/metabolismo , Anticorpos de Cadeia Única/biossíntese , Anticorpos/genética , Meios de Cultura/química , Meios de Cultura/metabolismo , Fermentação , Glicerol/metabolismo , Lipoproteínas LDL/imunologia , Pichia/genética , Pichia/crescimento & desenvolvimento , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Anticorpos de Cadeia Única/genética
17.
Work ; 54(4): 905-12, 2016 Jul 19.
Artigo em Inglês | MEDLINE | ID: mdl-27447416

RESUMO

BACKGROUND: Although comfort and discomfort on seats have been widely investigated, their nature is still not well described by literature and it is not known exactly how the interaction between these two phenomena happens. OBJECTIVE: To investigate the multidimensional nature of comfort and discomfort in aircraft seats as well as their levels of interaction. METHODS: A comfort-discomfort questionnaire has been given to 1500 passengers in Brazil. RESULTS: The results brought evidence that comfort and discomfort are different phenomena with a strong negative correlation. At extreme levels, the presence of comfort implies the absence of discomfort; however at the other levels of comfort (minimum to moderate) there is the presence of discomfort. Estimating the scores given by the passengers has allowed them to be placed at scales of comfort and discomfort with their different levels. However, no passenger has achieved the levels of maximum comfort or of maximum discomfort. CONCLUSIONS: It is possible to infer that comfort, as much as discomfort are multidimensional phenomena and must be evaluated through scales with different levels, since at the extreme levels there is no interaction between the phenomena, but at the intermediate levels the passengers could perceive comfort and discomfort at the same time.


Assuntos
Aeronaves/instrumentação , Aeronaves/normas , Ergonomia/normas , Percepção , Postura , Adulto , Brasil , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Inquéritos e Questionários
18.
Radiol Bras ; 48(5): 298-304, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26543281

RESUMO

OBJECTIVE: The present study was aimed at reviewing the literature to identify solutions for problems observed in radiology services. MATERIALS AND METHODS: Basic, qualitative, exploratory literature review at Scopus and SciELO databases, utilizing the Mendeley and Illustrator CC Adobe softwares. RESULTS: In the databases, 565 papers - 120 out of them, pdf free - were identified. Problems observed in the radiology sector are related to procedures scheduling, humanization, lack of training, poor knowledge and use of management techniques, and interaction with users. The design management provides the services with interesting solutions such as Benchmarking, CRM, Lean Approach, ServiceBlueprinting, continued education, among others. CONCLUSION: Literature review is an important tool to identify problems and respective solutions. However, considering the small number of studies approaching management of radiology services, this is a great field of research for the development of deeper studies.


OBJETIVO: O objetivo deste trabalho é levantar e identificar, na literatura, soluções no âmbito da gestão para os problemas encontrados na área de radiologia. MATERIAIS E MÉTODOS: A pesquisa é um estudo bibliográfico, de natureza básica, de abordagem qualitativa, do tipo exploratório. Foram utilizadas as bases de dados Scopus e SciELO e os softwares Mendeley e Illustrator CC Adobe. RESULTADOS: Nas bases de dados foram contabilizados, ao todo, 565 documentos, dos quais 120 tinham pdf free. Entre os problemas do setor estão: agendamento de horários, humanização, falta de treinamentos, pouco conhecimento e utilização das técnicas de gestão e interação com o usuário. A gestão de design apresenta soluções interessantes para o serviço, tais como Benchmarking, CRM, Lean Approach, ServiceBlueprinting, educação permanente, entre outras. CONCLUSÃO: Na busca de levantamento de problemas e soluções, a revisão de literatura é uma importante ferramenta, porém, tendo em vista os poucos estudos encontrados no âmbito de gestão na área, é importante realizar estudos mais profundos, o que torna a área um vasto campo a ser estudado.

19.
Radiol. bras ; Radiol. bras;48(5): 298-304, tab, graf
Artigo em Inglês | LILACS | ID: lil-764624

RESUMO

AbstractObjective:The present study was aimed at reviewing the literature to identify solutions for problems observed in radiology services.Materials and Methods:Basic, qualitative, exploratory literature review at Scopus and SciELO databases, utilizing the Mendeley and Illustrator CC Adobe softwares.Results:In the databases, 565 papers – 120 out of them, pdf free – were identified. Problems observed in the radiology sector are related to procedures scheduling, humanization, lack of training, poor knowledge and use of management techniques, and interaction with users. The design management provides the services with interesting solutions such as Benchmarking, CRM, Lean Approach, ServiceBlueprinting, continued education, among others.Conclusion:Literature review is an important tool to identify problems and respective solutions. However, considering the small number of studies approaching management of radiology services, this is a great field of research for the development of deeper studies.


ResumoObjetivo:O objetivo deste trabalho é levantar e identificar, na literatura, soluções no âmbito da gestão para os problemas encontrados na área de radiologia.Materiais e Métodos:A pesquisa é um estudo bibliográfico, de natureza básica, de abordagem qualitativa, do tipo exploratório. Foram utilizadas as bases de dados Scopus e SciELO e os softwares Mendeley e Illustrator CC Adobe.Resultados:Nas bases de dados foram contabilizados, ao todo, 565 documentos, dos quais 120 tinham pdf free. Entre os problemas do setor estão: agendamento de horários, humanização, falta de treinamentos, pouco conhecimento e utilização das técnicas de gestão e interação com o usuário. A gestão de design apresenta soluções interessantes para o serviço, tais como Benchmarking, CRM, Lean Approach, ServiceBlueprinting, educação permanente, entre outras.Conclusão:Na busca de levantamento de problemas e soluções, a revisão de literatura é uma importante ferramenta, porém, tendo em vista os poucos estudos encontrados no âmbito de gestão na área, é importante realizar estudos mais profundos, o que torna a área um vasto campo a ser estudado.

20.
Cad. saúde colet., (Rio J.) ; 22(4): 365-371, Oct-Dec/2014. graf
Artigo em Português | LILACS | ID: lil-741378

RESUMO

Da prescrição até a administração de medicamentos em pacientes podem ocorrer erros que causam dor, sofrimento e, em casos extremos, a morte. Assim, o objetivo geral deste artigo foi identificar os problemas relacionados aos utensílios da sala de preparo de medicação de um hospital da Grande Florianópolis no que tange à ergonomia física, cognitiva e organizacional, a fim de propor possíveis soluções no preparo de medicação para a eliminação ou minimização de erros no fluxo de medicação. Como método, fez-se: pesquisa bibliográfica, visita a campo e avaliação dos utensílios quanto à ergonomia física, cognitiva e organizacional. Como resultados, elaborou-se um quadro de análise de alguns utensílios da sala de medicação do hospital escola estudado, os quais foram relacionados com possíveis soluções para a otimização do processo de medicação. Assim, espera-se contribuir para a melhoria do trabalho dos profissionais da saúde e, consequentemente, promover uma melhor prestação de serviços à população.


From prescription to medication administration, errors that cause pain, suffering and, in extreme cases, death may occur in patients. Thus, the aim of this paper was to identify the problems related to the utensils used in the medication room of a hospital in Florianópolis, in respect to physical, cognitive and organizational ergonomics, in order to propose possible solutions to prepare medications for disposal or minimization of errors in the stream of medication. As a method, they were used: literature review, field visit and assessment tools as physical, cognitive and organizational ergonomics. As a results, it is presented a framework analysing some utensils of medication room, which has been elaborated and related with possible solutions to optimization of the medication process. Thus, it is expected to contribute to improving the work of health professionals and promote better service to the population.

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