RESUMO
The results of the development of oil palm (Elaeis guineensis Jacq.) microsatellite markers are given step by step, from the screening of libraries enriched in (GA)n, (GT)n, and (CCG)n simple-sequence repeats (SSRs) to the final characterisation of 21 SSR loci. Also published are primer sequences, estimates of allele size range, and expected heterozygosity in E. guineensis and in the closely related species E. oleifera, in which an optimal utility of the SSR markers was observed. Multivariate data analyses showed the ability of SSR markers to efficiently reveal the genetic-diversity structure of the genus Elaeis in accordance with known geographical origins and with measured genetic relationships based on previous molecular studies. High levels of allelic variability indicated that E. guineensis SSRs will be a powerful tool for genetic studies of the genus Elaeis, including variety identification and intra- or inter-specific genetic mapping. PCR amplification tests on a subset of 16 other palm species and allele-sequence data showed that E. guineensis SSRs are putative transferable markers across palm taxa. In addition, phenetic information based on SSR flanking region sequences makes E. guineensis SSR markers a potentially useful molecular resource for any researcher studying the phylogeny of palm taxa.
Assuntos
Evolução Molecular , Magnoliopsida/classificação , Magnoliopsida/genética , Repetições de Microssatélites/genética , Filogenia , África , Ásia , Sequência de Bases , Clonagem Molecular , Primers do DNA , DNA de Plantas/genética , Bases de Dados de Ácidos Nucleicos , Variação Genética , Biblioteca Genômica , Dados de Sequência Molecular , Reação em Cadeia da Polimerase , Polimorfismo Genético , Alinhamento de Sequência , Homologia de Sequência do Ácido Nucleico , América do SulRESUMO
Altogether my observations agree with the facts reported by others authors. Two data however should be pointed out: -- in spite of the progresses for its demonstration, lepra remains frequent in La Martinique; -- with 47% lepromatous forms and 25% tuberculoid forms, lepra in the West Indies constitutes an intermediary level between African lepra (60% tuberculoid forms) and South-American lepra (60% lepromatous forms).