RESUMO
O artigo apresenta parte dos resultados de uma investigação relativa ao projeto de cooperação internacional, envolvendo Brasil e Portugal, referente às diferenças e similitudes entre as políticas familiares de conciliação entre trabalho profissional e o trabalho familiar direcionadas as famílias com chefia feminina. A partir de levantamento bibliográfico, a revisão sistemática da literatura na área, identificam-se pontos de aproximação e distanciamento entre os autores estudados, como: à discussão da implantação das políticas restritivas de cariz neoliberal e desigualdades entre homens e mulheres; o debate sobre centralidade da família nas políticas sociais nos dois países em análise; as diferentes concepções de famílias e os novos arranjos familiares que se mesclam com o antigo padrão de família patriarcal e, finalmente, aborda as dificuldades da mulher chefe de família em conciliar formação, trabalho profissional e trabalho familiar no atual contexto econômico
The article presents part of the results of an investigation related to the international cooperation project, involving Brazil and Portugal, referring to the differences and similarities between family policies to reconcile professional work and family work directed at families with female heads. From a bibliographic survey, a systematic review of the literature in the area, points of approximation and distance between the authors studied are identified, such as: the discussion of the implementation of neoliberal restrictive policies and inequalities between men and women; the debate on the centrality of the family in social policies in the two countries under analysis; the different conceptions of families and the new family arrangements that blend with the old patriarchal family pattern and, finally, it addresses the difficulties of the female head of household in reconciling training, professional work and family work in the current economic context
Assuntos
Mulheres , Família , Política de Planejamento Familiar , Portugal , BrasilRESUMO
In this study we aimed to develop a roflumilast (R) and formoterol fumarate (F) dry powder inhaler formulation (DPI) incorporating HPßCD by spray drying and evaluated if it attenuates the inflammatory process and improves lung function in a murine model of ovalbumin induced allergic asthma. The DPI was characterized by powder X-ray diffraction, thermal analysis, scanning electron microscopy, particle size, density, specific surface area and dynamic vapor sorption analyses. In vitro deposition studies were performed using a NGI, while transepithelial permeability and in vivo effects on lung mechanics and inflammation in a model of allergic asthma were also assessed. The R:F formulation was amorphous with high glass transition temperatures, comprised of wrinkled particles, had low bulk and tapped densities, high surface area, suitable particle size for pulmonary delivery and exhibited no recrystallization even at high relative humidities. MMAD were statistically similar of 4.22 ± 0.19 and 4.32 ± 0.13 µm for F and R, respectively. Fine particle fractions (<5 µm) were of more than 50% of the emitted dose. The R:F formulation led to reduced eosinophil infiltration and airway collagen fiber content, yielding decreased airway hyperresponsiveness. In the current asthma model, the R:F formulation combination decreased inflammation and remodeling, thus improving lung mechanics.
Assuntos
Asma , Inaladores de Pó Seco , Administração por Inalação , Aminopiridinas , Animais , Asma/tratamento farmacológico , Benzamidas , Ciclopropanos , Fumarato de Formoterol/uso terapêutico , Camundongos , Tamanho da Partícula , Pós/uso terapêuticoRESUMO
The aim of this study was to develop roflumilast dry powder inhaler (DPI) formulations by spray drying using hydroxypropyl-ß-cyclodextrin (HPßCD) and to determine their suitability for pulmonary delivery. Different feed solution concentrations, solvent systems and spray drying parameters were used to obtain the formulations which were characterized using X-ray powder diffraction, thermal analysis, scanning electron microscopy, particle size distribution, bulk and tapped density, specific surface area, dynamic vapour sorption, in vitro deposition properties using a Next Generation Impactor (NGI) and transepithelial permeability. Microparticles spray dried from ethanol were wrinkled and amorphous, exhibiting high glass transition temperatures while those from methanol:n-butyl acetate consisted of irregularly shaped porous particles partially crystalline. All formulations presented low density, particle size and residual solvent content exhibiting high depositon in the lower stages of the NGI. Mass median aerodynamic diameters (MMADs) were in the range of 3.32-4.49⯵m, with high fine particle fractions (FPFâ¯<â¯5⯵m). Stability studies demonstrated no significant modifications in the solid-state nature and in the aerolisation performance of the selected formulation which presented a Papp of 8.73â¯×â¯10-6⯱â¯4.70â¯×â¯10-7â¯cm/s. The developed roflumilast DPI formulations have potential therapeutic applications in the treatment of lung diseases.
Assuntos
Aminopiridinas/química , Benzamidas/química , Composição de Medicamentos , Inaladores de Pó Seco , Administração por Inalação , Aminopiridinas/administração & dosagem , Aminopiridinas/farmacocinética , Anti-Inflamatórios/administração & dosagem , Anti-Inflamatórios/química , Anti-Inflamatórios/farmacocinética , Benzamidas/administração & dosagem , Benzamidas/farmacocinética , Linhagem Celular Tumoral , Ciclopropanos/administração & dosagem , Ciclopropanos/química , Ciclopropanos/farmacocinética , Sistemas de Liberação de Medicamentos , Humanos , Neoplasias Pulmonares/tratamento farmacológico , PósRESUMO
In this study, the formation of caffeine/dapsone (CAF/DAP) cocrystals by scalable production methods, such as liquid-assisted grinding (LAG) and spray drying, was investigated in the context of the potential use of processed cocrystal powder for pulmonary delivery. A CAF/DAP cocrystal (1:1 M ratio) was successfully prepared by slow evaporation from both acetone and ethyl acetate. Acetone, ethyl acetate, and ethanol were all successfully used to prepare cocrystals by LAG and spray drying. The powders obtained were characterized by X-ray diffractometry (XRD), differential scanning calorimetry (DSC), thermogravimetry (TGA), and Fourier transform infrared spectroscopy (FTIR). Laser diffraction analysis indicated a median particle size (D50) for spray-dried powders prepared from acetone, ethanol, and ethyl acetate of 5.4 ± 0.7, 5.2 ± 0.1, and 5.1 ± 0.0 µm respectively, which are appropriate sizes for pulmonary delivery by means of a dry powder inhaler. The solubility of the CAF/DAP cocrystal in phosphate buffer pH 7.4, prepared by spray drying using acetone, was 506.5 ± 31.5 µg/mL, while pure crystalline DAP had a measured solubility of 217.1 ± 7.8 µg/mL. In vitro cytotoxicity studies using Calu-3 cells indicated that the cocrystals were not toxic at concentrations of 0.1 and of 1 mM of DAP, while an in vitro permeability study suggested caffeine may contribute to the permeation of DAP by hindering the efflux effect. The results obtained indicate that the CAF/DAP cocrystal, particularly when prepared by the spray drying method, represents a possible suitable approach for inhalation formulations with applications in pulmonary pathologies.
Assuntos
Cafeína/análise , Cafeína/síntese química , Química Farmacêutica/métodos , Cristalização/métodos , Dapsona/síntese química , Administração por Inalação , Varredura Diferencial de Calorimetria/métodos , Linhagem Celular , Dapsona/análise , Dessecação/métodos , Composição de Medicamentos/métodos , Inaladores de Pó Seco , Humanos , Microscopia Eletrônica de Varredura/métodos , Tamanho da Partícula , Solubilidade , Espectroscopia de Infravermelho com Transformada de Fourier/métodos , Termogravimetria/métodos , Difração de Raios X/métodosRESUMO
The aim of this study was to develop and validate a discriminating in vitro release test to evaluate rivastigmine transdermal patches. The Exelon® Patch was chosen as a model transdermal product. The studies of in vitro release were designed to determine the impact of the official apparatus chosen (USP apparatus 5 and USP apparatus 6), the rotation speed, and the dissolution medium characteristics on the rivastigmine release profile from transdermal patches. Patches with different drug release profiles were tested in order to evaluate the discriminating power of the in vitro release test developed and validated. Variables such as the apparatus type, the dissolution medium, and the rotation speed have a significant influence on the drug release characteristics from a transdermal patch. The in vitro release methodologies using the USP apparatus 5 at 50 rpm and USP apparatus 6 at 25 rpm using the medium phosphate-buffered saline pH 7.4 were considered discriminative and adequate to characterize the rivastigmine (RV) release from a commercial transdermal patch, Exelon® Patch.
Assuntos
Liberação Controlada de Fármacos , Rivastigmina/administração & dosagem , Adesivo Transdérmico , Farmacopeias como Assunto , Rivastigmina/química , SolubilidadeRESUMO
In the present study, in vitro permeation experiments in a Franz diffusion cell were performed using different synthetic polymeric membranes and pig ear skin to evaluate a rivastigmine (RV) transdermal drug delivery system. In vitro-in vivo correlations (IVIVC) were examined to determine the best model membrane. In vitro permeation studies across different synthetic membranes and skin were performed for the Exelon(®) Patch (which contains RV), and the results were compared. Deconvolution of bioavailability data using the Wagner-Nelson method enabled the fraction of RV absorbed to be determined and a point-to-point IVIVC to be established. The synthetic membrane, Strat-M™, showed a RV permeation profile similar to that obtained with pig ear skin (R(2)=0.920). Studies with Strat-M™ resulted in a good and linear IVIVC (R(2)=0.991) when compared with other synthetic membranes that showed R(2) values less than 0.90. The R(2) for pig ear skin was 0.982. Strat-M™ membrane was the only synthetic membrane that adequately simulated skin barrier performance and therefore it can be considered to be a suitable alternative to human or animal skin in evaluating transdermal drug transport, potentially reducing the number of studies requiring human or animal samples.