RESUMO
20 pairs of sera from the National System of Seroepidemiological Surveillance of the triple viral vaccine received in the laboratory with febrile rash diagnosis were studied. By using the hemagglutination inhibition test, it was observed an abnormal answer of antibodies to both rubella and measles through a falling of the antibody titre in one or both diseases, or in one of them with seroconversion to the other one. With the aim of defining the existence of a possible polyclonal activation already described in literature, it was decided to study the antibody response to family Herpesviridae (HSV, EBV, CMV, VZV). 80% of answer to these viruses were found. The results are submitted and discussed.
Assuntos
Anticorpos Antivirais/imunologia , Infecções por Herpesviridae/imunologia , Herpesviridae/imunologia , Capsídeo/imunologia , Pré-Escolar , Citomegalovirus/imunologia , Exantema/virologia , Herpesvirus Humano 3/imunologia , Herpesvirus Humano 4/imunologia , Humanos , LactenteRESUMO
The most important clinical and epidemiological aspects of the lymphogranuloma venereum as a sexually-transmitted disease are described. We present a summary of the clinical history of an HIV-positive patient who presented with a tumoral lesion in the inguinal region presumptive of lymphogranuloma venereum. The diagnostic value of the polymerase chain reaction (PCR) technique for the establishment of an accurate diagnosis is stressed the epidemiological importance of the report of this sexually-transmitted disease in an HIV-positive patient for the first time in Cuba is also pointed out. A good response was attained with Doxycycline.
Assuntos
Soropositividade para HIV/complicações , Linfogranuloma Venéreo/complicações , Adulto , Cuba/epidemiologia , Humanos , Linfogranuloma Venéreo/epidemiologia , MasculinoRESUMO
An ELISA ultramicro was normalized for detecting herpes simplex virus antibodies. A study was made on 145 samples from the Pedro Kourí Tropical Medicine Institute and the Blood Bank, both by ELISA ultramicro and immunofluorescence and ELISA, and 98% and 99% of coincidence was reached, respectively. It indicates good correspondence between these techniques.
Assuntos
Anticorpos Antivirais/sangue , Ensaio de Imunoadsorção Enzimática/métodos , Técnica Indireta de Fluorescência para Anticorpo , Herpes Simples/imunologia , Simplexvirus/imunologia , Herpes Simples/sangue , Humanos , Sensibilidade e EspecificidadeRESUMO
Two methods for the diagnosis of rotavirus are compared with 120 stool samples from children under two years of age who had been hospitalized in Centro Habana Children's Hospital with a clinical diagnosis of acute diarrheal disease and 30 samples of healthy children of the same age group who served as controls. The methods used were latex agglutination (Rotalex, commercially-available kit from ORION DIAGNOSTIC ESPOO, Finland) and polyacrylamide gel electrophoresis. Electronic microscopy was used to define those cases whose results did not coincide with the previous techniques. Comparison was made based on the sensitivity and specificity, cost and easiness of performance. Rotalex was less specific but more sensitive than electrophoresis in polyacrylamide gel. The latter is less expensive, since reactants are available at a lower price in dollars and have no expiration date, is very useful in epidemiologic and outbreak studies, since it detects subgroups of circulating rotavirus and it is a painstaking technique (1-20 samples in 10 hours). Rotalex is simple, rapid (1-20 samples in 30 minutes) and allows individual determinations; therefore, we consider its application in hospitals is feasible.