Assuntos
Gnathostoma/isolamento & purificação , Gnathostoma/ultraestrutura , Pescoço , Dermatopatias Parasitárias/diagnóstico , Dermatopatias Parasitárias/parasitologia , Infecções por Spirurida/diagnóstico , Infecções por Spirurida/parasitologia , Adulto , Animais , Diagnóstico Diferencial , Feminino , Humanos , Masculino , México , Dermatopatias Parasitárias/patologia , Infecções por Spirurida/patologiaRESUMO
An 11-year-old girl presented from Villa Azueta, Veracruz, a poor and small town on the Tesechoacan riverside. The Tesechoacan riverside is a tropical area in the south-east of Mexico, between 18 degrees, 04',32" latitude and 95 degrees, 42',23" longitude. Her family consisted of both parents and 12 brothers, all apparently healthy and living in a humble hut, with poor nutrition and hygiene. The main sign was a tumor on her neck that had developed during the previous 6 months. On clinical examination, the patient's temperature was normal and her weight was 27.5 kg. Below the right angle of the jaw a firm-to-hard tumor was noted measuring 5 x 3 cm, it was tender and lobulated (Fig. 1) with a central purulent fistula containing small worms with morphology of Lagochilascans minor (Fig. 2). Iron-deficiency anemia was found with no increase of blood eosinophils and a while blood count of 9000/mm3 with 72% of neutrophils. Roentgenograms of the head and chest were both normal. A stool was negative for parasites; glutamic oxalade and glutamic pyruvate transaminases were normal. Histopathologic examination revealed parakeratosis and mild acanthosis with exocytosis of eosinophils. The superficial dermis showed an inflammatory reaction, mainly composed of eosinophils, plasma cells, and histiocytes, and in the deep dermis an abscess containing different sections of the parasite was seen surrounded by a thin fibrous capsule (Fig. 3). There was no clinical improvement after medical treatment with two courses of thiabendazole (50 mg/kg orally, two 5-day pulses) and praziquantel (400 mg orally for 3 days). Therefore, the growth was extirpated under general anesthesia; however, the clinical condition of the patient did not improve. Eggs, larvae, and adult worms continued to be present in the pus after surgical excision. Finally, the patient asked for a discharge and did not return to the institute.
Assuntos
Infecções por Ascaridida , Ascaridoidea , Animais , Infecções por Ascaridida/diagnóstico , Infecções por Ascaridida/parasitologia , Infecções por Ascaridida/cirurgia , Criança , Feminino , Humanos , Larva , Pescoço/parasitologiaRESUMO
A comparative study of two different staining methods for estimation of yeast's viability is assessed by microbial flow cytometry. Propidium iodide (PI) a well known nucleic-acid binding vital dye exclusion is compared with light green (LG), a cytoplasmic function related vital dye widely used in histological, histochemical and immunocytochemical staining. The yeast model used was heat-killed Candida guilliermondii cells. The stained heat-killed bacterial population showed on the dot-plot flow cytometer analysis, a clear separation towards the green-orange (350-600 nm) for LG, and orange-red (550-725 nm) for PI on the fluorescence spectrum-zone channels. Both dyes showed by non-parametric Kolmcgorov-Smirnov, 99% of probability of difference between heat-killed and live microorganism's histogram-population, with a D = ranking from 0.50-0.64 for LG to 0.91-0.98 for PI. Higher cost and carcinogenic risk when handling PI, make LG a better choice for flow cytometry yeast viability tests.
Assuntos
Candida/citologia , Citometria de Fluxo , Corantes Fluorescentes , Verde de Metila , Coloração e Rotulagem/métodos , Candida/metabolismo , Permeabilidade da Membrana Celular , Corantes Fluorescentes/metabolismo , Verde de Metila/metabolismo , Propídio/metabolismoRESUMO
We propose that the ratios of lymphocyte subsets: CD4+/CD8+, CD4+/CD3+, and CD8+/CD3+, graphically displayed in this order, could be an easy way of immunophenotyping and a simple form of interpretation both for clinicians and patients. A total of 187 asymptomatic HIV-positive patients, -including 83 symptomatic patients with AIDS- and a normal comparative age group of 36 patients, were studied by flow cytometry. Ratios were graphically displayed on millimetric-grid paper, and a specific point was given to each obtained ratio. The points were connected by a line. The angles formed by the lines were measured and graphically displayed over a circle. The point of reference 0 degree was located on the left side of the circle, giving the corresponding 90 degrees, 180 degrees, and 240 degrees angles. The graph of the control group shows a "beach chair" image. As soon as the CD4+ lymphocytes diminish and the CD8+ lymphocytes increase in HIV+ patients, "the chair's headrest" descends and the "chair's footpiece" swivels back. Given the ranks and fluctuations of the lymphocyte subsets, their graphic display provides a better representation of the immunological status.
Assuntos
Soropositividade para HIV/imunologia , HIV-1/imunologia , Imunofenotipagem/métodos , Subpopulações de Linfócitos/classificação , Adulto , Análise de Variância , Relação CD4-CD8 , Feminino , Citometria de Fluxo/instrumentação , Citometria de Fluxo/métodos , Citometria de Fluxo/estatística & dados numéricos , Humanos , Imunofenotipagem/instrumentação , Imunofenotipagem/estatística & dados numéricos , Subpopulações de Linfócitos/imunologia , Masculino , Pessoa de Meia-IdadeRESUMO
A new serogroup of Vibrio cholerae non-O1, O:139, has been implicated in recent epidemics. It was scanned with a factor A-specific fluoresceine-conjugated monoclonal antibody, searching for antigen determinants by laser flow cytometry. First, a group of gram-negative 4-amine-4, 6 dideoxy-D-mannose antigen-related microorganisms were tested to assess monoclonal antibody cross reactions. Later, a clear recognition of antigen determinants was found with this monoclonal antibody, on V. cholerae non-O1, O:136, Bengal, and MO45 strains, showing no cross reactions with the antigenically related non O1, O:22, and Inaba and Ogawa O1 strains. On the other hand, factor A of V. cholerae O1 strains was recognized by the specific monoclonal antibody and a discrete factor A on V. cholerae non-O1, O:139, Bengal and MO45 strains was detected.
Assuntos
4-Butirolactona/análogos & derivados , Citometria de Fluxo/métodos , Substâncias de Crescimento/análise , Vibrio cholerae/classificação , 4-Butirolactona/análise , 4-Butirolactona/biossíntese , Substâncias de Crescimento/biossíntese , Humanos , Lasers , Sorotipagem , Vibrio cholerae/metabolismoRESUMO
A total of 72 peptonated water samples suspected of carrying Vibrio cholerae were assessed by laser flow cytometry (LFC) and compared with positive culture. We used a direct fluorescence technique using polyclonal (PolAb) and monoclonal antibodies (MoAb) conjugated to fluorescein. The PolAb were able to detect 33 positive samples. A clear difference among the 20 positive samples was found with only three V. cholerae O1 false negatives when MoAb were used whereas all 13 V. cholerae Non O1 samples were detected. The correlation index comparing control autofluorescence with peptonated water samples show a R = 0.69, versus 0.96 with pure V. cholerae O1 strains. Our data suggest that the LFC technique is able to recognize V. cholerae O1 from a mixture of microorganisms with high sensitivity and specificity in a few hours.
Assuntos
Cólera/microbiologia , Fezes/microbiologia , Citometria de Fluxo , Vibrio cholerae/isolamento & purificação , Anticorpos Antibacterianos/imunologia , Anticorpos Monoclonais/imunologia , Antígenos de Bactérias/imunologia , Método Duplo-Cego , Fluoresceína , Fluoresceínas , Microbiologia de Alimentos , Humanos , Lipopolissacarídeos/imunologia , Sensibilidade e Especificidade , Sorotipagem , Vibrio cholerae/classificação , Vibrio cholerae/imunologia , Microbiologia da ÁguaAssuntos
Infecções por HIV/diagnóstico , HIV-1 , Serviços de Diagnóstico , Humanos , Lactente , Recém-Nascido , MéxicoRESUMO
Two lipopolysaccharide antigens that differ by the technique of preparation from S. typhi, one named crude is easily extracted, the other one is water-phenol extracted. Both were adsorbed by papain and cysteine to lamb erythrocyte surfaces, fixed then by glutaraldehyde and used in a plate microhemagglutination test (MHA). The MHA test was done with 30 sera from children with S. typhi blood isolates. The same number of control sera were tested for comparison. The crude LPS antigen showed better sensitivity: 73% versus 53% of the water-phenol extracted one despite a high index correlation (r = 0.88). The latest one was evaluated in a prospective study, using it during a six-month period in an emergency room with S. typhi blood isolated sera. The MHA test was compared with the surface fixation test. Both assays showed similar sensitivity and a specificity of 91 and 100%, respectively.
Assuntos
Febre Tifoide/imunologia , Adolescente , Criança , Pré-Escolar , Feminino , Testes de Hemaglutinação/métodos , Humanos , Lipopolissacarídeos/imunologia , Masculino , Salmonella typhi/imunologia , Febre Tifoide/sangueRESUMO
Using a capillary flocculation technique we evaluated serum samples of asymptomatic children for typhoid fever serology. Thirty one (5.16%) of the 600 serum samples tested were positive for a specificity of 95%. To evaluate the sensitivity of the test, serum samples from 36 children with proven typhoid fever and a similar number of control patients were evaluated. The sensitivity of the capillary flocculation test in this group was 100%. The test compared favorably with the Ruiz Castañeda serologic test for typhoid fever.