RESUMO

A coronectomia consiste no procedimento em que se faz a remoção da coroa do dente deixando a raiz in situ. Tal técnica é indicada para dentes posteriores impactados que apresentem proximidade com o canal mandibular representando assim um risco ao nervo mandibular caso seja feita uma exodontia. O seguinte trabalho tem como objetivo demonstrar os aspectos positivos relacionados ao emprego da técnica de coronectomia para dentes posteriores impactados. Foram realizadas buscas bibliográficas nas bases de dados PUBMED, MEDLINE e sciELO usando os descritores "coronectomia", "coronectomy", "terceiros molares impactados", "trigeminal", "nerve damage", respeitando critérios de relevância quanto ao tema foram selecionados 31 artigos. Sabe-se que pelo canal mandibular passa o nervo alveolar inferior e esse é responsável pela inervação sensitiva de estruturas anatômicas importantes, logo dentes impactados que tenham grande proximidade com o canal mandibular quando empregadas técnicas de exodontia correm o risco de lesionar o nervo podendo gerar perda da sensibilidade de forma passageira ou permanente, a indicação para realização da técnica precisa ser feita posteriormente à análise radiográfica e tomográfica da relação do dente com o canal mandibular. A técnica consiste na remoção da coroa do dente e o suficiente da raiz abaixo da crista vestibular e lingual do osso, para que esse possa desencadear um processo de cicatrização natural sobre as raízes retidas. Conclui-se que a coronectomia é um procedimento alternativo seguro e eficaz para casos específicos que se tem indicação e apresenta prognóstico favorável.

The coronectomy consists of the procedure in which the crown of the tooth is removed leaving the root in situ. This technique is indicated for impacted posterior teeth in close proximity to the mandibular canal representing a risk to the mandibular nerve if an extraction is performed. The following study has the porpouse to demonstrate the positive aspects related to the use of the coronectomy technique for impacted posterior teeth. Bibliographic searches were carried out in the databases PUBMED, MEDLINE, sciELO, using the DeCS "coronectomy", "odontectomy", "trigeminal", "nerve damage", respecting the criterion of relevance to the topic, 31 articles were selected. It is known that the inferior alveolar nerve passes through the mandibular canal and is responsible for the sensitive innervation of important anatomical structures, so impacted teeth that are very close to the mandibular canal when extraction techniques are used has the risk of injuring the nerve, which may lead to temporary or permanent loss of sensitivity, the indication for performing the technique needs to be made after the radiographic and tomographic analysis of the affinity between the tooth and the mandibular canal. The technique consists of removing the crown of the tooth and enough of the root below the buccal and lingual crest of the bone, so that it can trigger a natural healing process on the retained roots. It is concluded that coronectomy is a safe and effective alternative procedure for specific cases that are indicated and have a favorable prognosis.

Assuntos

RESUMO

INTRODUCTION: Metagenomic sequencing is a powerful tool that is widely used in laboratories worldwide for taxonomic characterization of microorganisms in clinical and environmental samples. In this study, we utilized metagenomics to investigate comprehensively the microbial diversity in fecal samples of children over a four-year period. Our methods were carefully designed to ensure accurate and reliable results. MATERIAL AND METHODS: Validated and analyzed were metagenomic data obtained from sequencing 27 fecal samples from children under 10 years old with gastroenteritis over a four-year period (2012-2016). The fecal specimens were collected from patients who received care at public health facilities in the northern region of Brazil. Sequencing libraries were prepared from cDNA and sequenced on the Illumina HiSeq. Kraken-2 was utilized to classify bacterial taxonomy based on the 16S rRNA gene, using the Silva rRNA database. Additionally, the Diamond program was used for mapping to the non-redundant protein database (NR database). Phylogenomic analyses were conducted using Geneious R10 and MEGA X software, and Bayesian estimation of phylogeny was performed using the MrBayes program. The results indicate significant heterogeneity among norovirus strains, with evidence of recombination and point mutations. This study presents the first complete genome of parechovirus 8 in the region. Additionally, it describes the bacterial populations and bacteriophages present in feces, with a high abundance of Firmicutes and Proteobacteria, including an increased proportion of the Enterobacteriaceae family. The presented data demonstrate the genetic diversity of microbial populations and provide a comprehensive report on viral molecular characterization. These findings are relevant for genomic studies in gastrointestinal infections. The metagenomic approach is a powerful tool for investigating microbial diversity in children with gastroenteritis. However, further studies are imperative to conduct genomic analysis of identified bacterial strains and thoroughly analyze antimicrobial resistance genes.

RESUMO

BACKGROUND: Next-generation sequencing has had a significant impact on genetic disease diagnosis, but the interpretation of the vast amount of genomic data it generates can be challenging. To address this, the American College of Medical Genetics and Genomics and the Association for Molecular Pathology have established guidelines for standardized variant interpretation. In this manuscript, we present the updated Hospital Israelita Albert Einstein Standards for Constitutional Sequence Variants Classification, incorporating modifications from leading genetics societies and the ClinGen initiative. RESULTS: First, we standardized the scientific publications, documents, and other reliable sources for this document to ensure an evidence-based approach. Next, we defined the databases that would provide variant information for the classification process, established the terminology for molecular findings, set standards for disease-gene associations, and determined the nomenclature for classification criteria. Subsequently, we defined the general rules for variant classification and the Bayesian statistical reasoning principles to enhance this process. We also defined bioinformatics standards for automated classification. Our workgroup adhered to gene-specific rules and workflows curated by the ClinGen Variant Curation Expert Panels whenever available. Additionally, a distinct set of specifications for criteria modulation was created for cancer genes, recognizing their unique characteristics. CONCLUSIONS: The development of an internal consensus and standards for constitutional sequence variant classification, specifically adapted to the Brazilian population, further contributes to the continuous refinement of variant classification practices. The aim of these efforts from the workgroup is to enhance the reliability and uniformity of variant classification.

Assuntos

RESUMO

Objetivou-se compreender os motivos da adesão ou não do participante ao uso da máscara em caso de ter alguma "gripe"; conhecer as ações realizadas pelos participantes para evitar a transmissão quando apresentavam os sintomas de gripe antes da pandemia; identificar a opinião dos participantes sobre o usos de máscara antes da pandemia; verificar as sensações quanto ao uso de máscara facial na pandemia e averiguar a aceitação da população quanto ao uso de máscara facial em caso de sintomas de doenças respiratórias infectocontagiosas na pós-pandemia. Consiste-se em um estudo descritivo, exploratório, com abordagem qualitativa, realizado com 62 participantes usuários de transporte coletivo. Utilizou-se entrevistas semiestruturadas no mês de outubro de 2020. Os dados coletados foram submetidos à análise segundo a fenomenologia social e categorizados conforme abordagem fenomenológica. As motivações para usar a máscara em locais públicos em caso de gripe na pós-pandemia, mostram a preocupação com a prevenção e a proteção consigo e com outros. A não adesão refere-se à dificuldade no convívio social e o desconforto. Assim, compreende-se que o autocuidado esteve presente entre vários discursos dos participantes e que quase a totalidade dos participantes, em caso de "gripe" utilizarão a máscara depois da pandemia. Conclui-se que a educação da população relacionada ao uso de máscara facial na pós-COVID-19, em locais públicos, visando diminuir a disseminação e a contaminação pelas doenças respiratórias infectocontagiosas é primordial.

The objective of this study was to understand the reasons for the participant's adherence or not to the use of mask in case of catching a "flu"; to analyze the actions taken by the participants to avoid transmission when they had flu symptoms before the pandemic; identify the opinion of the participants on the use of masks before the pandemic; to verify the general feeling towards the use of a face mask in the pandemic, and to verify the acceptance of the population regarding the use of a face mask in case of symptoms of infectious and contagious respiratory diseases in post-pandemic times. This consists of a descriptive, exploratory study, with a qualitative approach, carried out with 62 participants who use public transportation. Semi-structured interviews were used in October 2020. The data collected were subjected to analysis according to social phenomenology and categorized according to a phenomenological approach. The motivations for wearing the mask in public places in case of flu in post-pandemic times show the concern with prevention and protection for themselves and others. Non-adherence refers to difficulty in socializing and discomfort. Thus, it is understood that self-care was present among several answers and that almost all the participants, in case of a "flu", will wear a mask after the pandemic. It is concluded that instructing the population regarding the use of a face mask in public places in post-COVID-19 times in order to reduce the spread and contamination by infectious and contagious respiratory diseases is of paramount importance.