Assuntos
Anormalidades Múltiplas , Adenofibroma/complicações , Neoplasias da Mama/complicações , Comunicação Interatrial/complicações , Artéria Pulmonar/anormalidades , Exoftalmia/complicações , Feminino , Humanos , Lactente , Rim/anormalidades , Rim/diagnóstico por imagem , Macroglossia/complicações , Radiografia , SíndromeRESUMO
Two methods for the assay of rifampicin in serum are described. The first is a conventional plate diffusion method, measuring concentrations down to 0,02 ug/ml, and the second a chemical extraction followed by measurement of the inhibition of uptake of 14C-uridine by Staphylococcus aureus, which estimetes in the range of 0,02 to 0,001 ug/ml. The methods were ussed to measure serum concentrations in man following doses of about 1050 mg and 75 mg rifampicin.
Assuntos
Humanos , Bioensaio/métodos , Mycobacterium tuberculosis , Radioisótopos de Carbono , Rifampina/farmacologia , Rifampina/isolamento & purificação , Rifampina/sangue , Staphylococcus , Staphylococcus/metabolismoRESUMO
Rifampicin in urine was detected by a simple plate diffusion assay using as the test organism a strain of Staphylococcus aureus made resistant to other antituberculosis drugs. The specificity of the method was ensured by a parallel test with a rifampicin-resistant variant of this strain. The method was more sensitive than available chemical techniques and yieldeld reliably positive results for at least twelve hours after a 600 mg. dose of rifampicin.