RESUMO

We study a new arthropod cell line, AP-64, which was obtained from Aedes pseudoscutellaris larvae. The best growth and maintenance media are defined, testing the adequacy of dengue 1 and 2 viruses which become manifest by the appearance of the cytopathogenic effect as syncytiums. The immunofluorescence technique is applied to detect early multiplication of those viruses.

Assuntos

Aedes , Vírus da Dengue/fisiologia , Replicação Viral , Aedes/virologia , Animais , Linhagem Celular , Efeito Citopatogênico Viral , Imunofluorescência , Larva/virologia , Cultura de Vírus/métodos