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The aim of this study was the molecular imprinting polymers (MIPs) assessment as a controlled release system of ciprofloxacin. The MIPs synthesis was performed by three different methods: emulsion, bulk, and co-precipitation. Lactic acid (LA) and methacrylic acid (MA) were used as functional monomers and ethylene glycol dimethacrylate as crosslinker. Also, nonimprinted polymers (NIPs) were synthesized. MIPs and NIPs were characterized by scanning electron microscopy, Fourier Transform Infrared Reflection, specific surface area, pore size, and release kinetics. Their efficiency against Staphylococcus aureus and Escherichia coli, and their cytotoxicity in dermal fibroblast cells were proven. Results show that MIPs are mesoporous materials with a pore size between 10 and 20 nm. A higher adsorption with the co-precipitation MIP with MA as a monomer was found. The release kinetics proved that a non-Fickian process occurred and that the co-precipitation MIP with LA presented the highest release rate (90.51 mg/L) in 8 h. The minimum inhibitory concentration was found between 0.031 and 0.016 mg/L for Staphylococcus aureus and between 0.004 and 0.031 mg/L for the Escherichia coli. No cytotoxicity in cellular cultures was found; also, cellular growth was favored. This study demonstrated that MIPs present promising properties for drug administration and their application in clinical practice.
Assuntos
Metacrilatos , Impressão Molecular , Polímeros Molecularmente Impressos , Preparações de Ação Retardada , Ciprofloxacina/farmacologia , Polímeros , Impressão Molecular/métodos , Escherichia coli , AdsorçãoRESUMO
BACKGROUND: Bovine pericardium (BP) is a scaffold widely used in soft tissues regeneration; however, its calcification in contact with glutaraldehyde, represent an opportunity for its application in hard tissues, such as bone in the oral cavity. OBJECTIVE: To develop and to characterize decellularized and glutaraldehyde-crosslinked bovine pericardium (GC-BP) as a potential scaffold for guided bone regeneration GBR. METHODS: BP samples from healthy animals of the bovine zebu breed were decellularized and crosslinked by digestion with detergents and glutaraldehyde respectively. The resulting cell-free scaffold was physical, chemical, mechanical, and biologically characterized thought hematoxylin and eosin staining, DNA quantification, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), uniaxial tensile test, cell viability and live and dead assay in cultures of dental pulp stem cells (DPSCs). RESULTS: The decellularization and crosslinking of BP appeared to induce conformational changes of the CLG molecules, which led to lower mechanical properties at the GC-BP scaffold, at the same time that promoted cell adhesion and viability of DPSCs. CONCLUSION: This study suggests that the decellularized and GC-BP is a scaffold with the potential to be used promoting DPSCs recruitment, which has a great impact on the dental area.
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Calcificação Fisiológica , Pericárdio , Bovinos , Animais , Glutaral/análise , Glutaral/farmacologia , Adesão Celular , Alicerces Teciduais/químicaRESUMO
The objective of this work was to evaluate the use of an electronic nose and chemometric analysis to discriminate global patterns of volatile organic compounds (VOCs) in breath of postCOVID syndrome patients with pulmonary sequelae. A cross-sectional study was performed in two groups, the group 1 were subjects recovered from COVID-19 without lung damage and the group 2 were subjects recovered from COVID-19 with impaired lung function. The VOCs analysis was executed using a Cyranose 320 electronic nose with 32 sensors, applying principal component analysis (PCA), Partial Least Square-Discriminant Analysis, random forest, canonical discriminant analysis (CAP) and the diagnostic power of the test was evaluated using the ROC (Receiver Operating Characteristic) curve. A total of 228 participants were obtained, for the postCOVID group there are 157 and 71 for the control group, the chemometric analysis results indicate in the PCA an 84% explanation of the variability between the groups, the PLS-DA indicates an observable separation between the groups and 10 sensors related to this separation, by random forest, a classification error was obtained for the control group of 0.090 and for the postCOVID group of 0.088 correct classification. The CAP model showed 83.8% of correct classification and the external validation of the model showed 80.1% of correct classification. Sensitivity and specificity reached 88.9% (73.9%-96.9%) and 96.9% (83.7%-99.9%) respectively. It is considered that this technology can be used to establish the starting point in the evaluation of lung damage in postCOVID patients with pulmonary sequelae.
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COVID-19 , Compostos Orgânicos Voláteis , Humanos , Estudos Transversais , Testes Respiratórios/métodos , COVID-19/diagnóstico , Pulmão/química , Sensibilidade e Especificidade , Expiração , Nariz Eletrônico , Compostos Orgânicos Voláteis/análiseRESUMO
Environmental pollutants are involved in the development and progression of numerous cancers, including cervical cancer (CC). One possible explanation for this is the ability of several pollutants to mimic natural hormones. This study aimed to evaluate the urinary concentrations of monoesters of phthalates and bisphenol A (BPA) in women with CC. A total of 45 women were included: 15 in the control group, 12 with CC diagnosis classified in early stages IA-IIB, and 18 in late stages III-IV. Urine samples were analyzed for BPA, mono-isobutyl phthalate (MiBP), mono-n-butyl phthalate (MBP), monobenzyl phthalate (MBzP), and mono 2-ethylhexyl phthalate (MEHP) using high-performance liquid chromatography coupled to a tandem mass detector. The detection rate of environmental pollutants was 100%, with a median concentration in the control group and early-, and late-stage groups of 10.4, 9.2, 4.3, 38.4, and 12.9 µg L-1; 3.1, 3.1, 151.1, 54.5, and 30.4 µg L-1 and 1.9, 92.8, 3.6, 31.0, and 9.3 µg L-1 for BPA, MEHP, MBzP, MBP, and MiBP, respectively This study reveals high levels of phthalates, particularly MEHP, in urine samples of women with CC associated with human papillomavirus (HPV) infection. Further studies are needed to evaluate the possible role of phthalates in synergy with HPV in progression to CC.
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Poluentes Ambientais , Infecções por Papillomavirus , Ácidos Ftálicos , Neoplasias do Colo do Útero , Humanos , Feminino , Ácidos Ftálicos/metabolismo , Poluentes Ambientais/metabolismo , Exposição Ambiental/análiseRESUMO
Purpose: Exosomes are extracellular membrane vesicles. Their content directly reflects the metabolic state of the cells from which they originate and play an important role in cellular functions and pathological states, for example, cancer. The aim was to establish the effect of exosomes from patients diagnosed with CIN1 (grade one cervical intraepithelial neoplasia) on the viability of HeLa cells in culture. It had not been documented, nor had the vesicles obtained by cervicovaginal samples taken by the patients themselves (self-taken vaginal). Patients and Methods: Exosomes were obtained from self-taken vaginal by patients diagnosed with CIN1 and healthy. The exosomes were characterized by determining the AChE (acetylcholinesterase) activity, obtaining a protein profile, and obtaining images of these by STEM. The effect on cell viability was made in HeLa and HaCaT cells in culture. Results: Vesicles between 185 nm and 415 nm were observed by STEM. Exosomes show a "protective" effect when those patients without injury are confronted with HeLa cells. On the other hand, exosomes promote viability when they come from injured patients in the presence of the same cells. Conclusion: Exosomes can be used to identify ideal biomarkers for the early diagnosis of CC (cervical cancer), follow-up of patients, and even treatment given the effects observed on cell cultures.
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The quarrying is considered a precarious occupation with high toxicity, is an informal economic activity that employs low technology, limited protection, and poses a risk to workers and their families. In quarrying, silica dust is generated and there is also occupational exposure to significant mixtures of pneumotoxic pollutants, including mineral dust (crystalline silica, carbon or cement, polycyclic aromatic hydrocarbons (PAHs), solvents, and others, which are aggravated by the lack of use of protective equipment, causing irreversible damage to the worker's respiratory health. Thus, the objective of this work focused on the evaluation of the respiratory health of artisan stonemasons in San Luis Potosí, Mexico through the study of exhaled breath condensate (EBC) (pH, pro-inflammatory cytokines) as well as the study of the exposure to pollutants present in the work area (PAHs, toluene, and 2.5 µm particulate matter) through biomarkers of exposure (hippuric acid and hydroxylated metabolites of PAHs). The results show the presence of crystalline SiO2 in 100% of the samples analyzed; the PM2.5 concentrations were 5 to 10 times the permitted levels. Regarding exposure to PAHs, all the stonemasons presented urine concentrations of at least 5 of the OH-PAHs evaluated; 9-OH-FLU occurred at higher concentrations of 171.2 (122.7-279.4) µg L-1; hippuric acid, which was present in 100% of the workers evaluated in concentrations of 283.4 (27.72-1119) mg L-1, 100% of which were above the values established for occupational scenarios. The pH values obtained for the EBC samples were presented at an average of 7.07 (6.33-7.66). Pro-inflammatory cytokines were present in 86.1% of the study population. The cytokine that was found in higher concentrations was IL-2, with a mean of 178.01 pg mL-1 and 3124.01 pg mL-1 for the pH < 7 and pH > 7 groups, respectively. Some correlations between the cytokines and the exposure biomarkers were presented. Stonemasons are highly exposed to pneumotoxic pollutants and markers of inflammation at the pulmonary level; in addition, a high risk of developing silicosis. Quarrying should be addressed as a carcinogenic activity, which would imply the design of monitoring and control strategies for these pollutants that our country currently lacks, particularly in precarious occupations. It is necessary to develop strategies to protect the health of precarious workers.
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Poluentes Ambientais , Exposição Ocupacional , Hidrocarbonetos Policíclicos Aromáticos , Biomarcadores/metabolismo , Citocinas , Poeira , Monitoramento Ambiental/métodos , Humanos , Exposição Ocupacional/análise , Hidrocarbonetos Policíclicos Aromáticos/análise , Dióxido de SilícioRESUMO
Struthanthus quercicola, a hemiparasitic plant known as "seca palo," is used by Nahuatl traditional healers against diabetes, wounds, and rashes. We aimed to investigate the effects of different S. quercicola extracts, which were selected based on their traditional use in Tamazunchale, San Luis Potosí, on the cell viability and antioxidant activity in HeLa cell cultures. S. quercicola growing on Guazuma ulmifolia and Citrus sp. hosts was collected, and methanolic and ethanolic extracts as well as decoctions, infusions, and microwave-assisted extracts were obtained. The terpenoid, alkaloid, flavonoid, saponin, and tannin contents of each extract were evaluated qualitatively and quantitatively. The effects of different extracts on the viability of cervical adenocarcinoma (HeLa) cells were tested using an MTT assay. The differences in the total flavonoid and phenolic contents and free-radical scavenging activity in relation to the host and the extract were also determined. In assessments of the effects of the extracts on cell viability, eight organic extracts (4 from G. quercicola grown on Host 1 and 4 from G. quercicola grown on Host 2) were shown to decrease cell viability significantly in comparison with the control. However, the extract obtained by percolation (PMeOH) caused a significant increase in cell viability (p < 0.05), especially with the plant grown on Host 1. The microwave aqueous and methanolic extracts of the plants grown on both hosts showed a significant increase in the percentage of apoptosis (p < 005). In conclusion, different extracts of Struthanthus quercicola showed variable effects on cell viability and apoptosis. Isolation of the molecule or molecules with inhibitory and proliferative effects on cells should be conducted to evaluate their possible use as antineoplastic agents.
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The general aim of this study was to evaluate physicochemical properties, prebiotic activity and anticancer potential of jackfruit (Artocarpus heterophyllus) seed flour. The drying processes of jackfruit seeds were performed at 50, 60 and 70 °C in order to choose the optimal temperature for obtaining the flour based on drying time, polyphenol content and antioxidant capacity. The experimental values of the moisture ratio during jackfruit seed drying at different temperatures were obtained using Page's equation to establish the drying time for the required moisture between 5 and 7% in the flour. The temperature of 60 °C was considered adequate for obtaining good flour and for performing its characterization. The chemical composition, total dietary fiber, functional properties and antioxidant capacity were then examined in the flour. The seed flour contains carbohydrates (73.87 g/100 g), dietary fiber (31 g/100 g), protein (14 g/100 g) and lipids (1 g/100 g). The lipid profile showed that the flour contained monounsaturated (4 g/100 g) and polyunsaturated (46 g/100 g) fatty acids. Sucrose, glucose, and fructose were found to be the predominant soluble sugars, and non-digestible oligosaccharides like 1-kestose were also found. The total polyphenol content was 2.42 mg of gallic acid/g of the sample; furthermore, the antioxidant capacity obtained by ferric reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) was 901.45 µmol Trolox/100 g and 1607.87 µmol Trolox/100 g, respectively. The obtained flour exhibited good functional properties, such as water and oil absorption capacity, swelling power and emulsifier capacity. Additionally, this flour had a protective and preventive effect which is associated with the potential prebiotic activity in Lactobacillus casei and Bifidobacterium longum. These results demonstrate that jackfruit seed flour has good nutritional value and antioxidant and prebiotic activity, as well as potential protective effects and functional properties, making it an attractive food or ingredient in developing innovative functional products.
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Antineoplásicos/farmacologia , Antioxidantes/farmacologia , Artocarpus/química , Fenômenos Químicos , Farinha/análise , Prebióticos , Sementes/química , Catalase/metabolismo , Morte Celular/efeitos dos fármacos , Linhagem Celular Tumoral , Cromatografia Líquida de Alta Pressão , Dessecação , Células Epiteliais/efeitos dos fármacos , Células Epiteliais/metabolismo , Humanos , Umidade , Cinética , Lipídeos/análise , Extratos Vegetais/farmacologia , Polifenóis/análise , Solubilidade , Espectroscopia de Infravermelho com Transformada de Fourier , Superóxido Dismutase/metabolismo , Viscosidade , Água/químicaRESUMO
BACKGROUND: We identified a global chemical pattern of volatile organic compounds in exhaled breath capable of discriminating between COVID-19 patients and controls (without infection) using an electronic nose. METHODS: The study focused on 42 SARS-CoV-2 RT-qPCR positive subjects as well as 42 negative subjects. Principal component analysis indicated a separation of the study groups and provides a cumulative percentage of explanation of the variation of 98.3%. RESULTS: The canonical analysis of principal coordinates model shows a separation by the first canonical axis CAP1 (r2 = 0.939 and 95.23% of correct classification rate), the cut-off point of 0.0089; 100% sensitivity (CI 95%:91.5-100%) and 97.6% specificity (CI 95%:87.4-99.9%). The predictive model usefulness was tested on 30 open population subjects without prior knowledge of SARS-CoV-2 RT-qPCR status. Of these 3 subjects exhibited COVID-19 suggestive breath profiles, all asymptomatic at the time, two of which were later shown to be SARS-CoV-2 RT-qPCR positive. An additional subject had a borderline breath profile and SARS-CoV-2 RT-qPCR positive. The remaining 27 subjects exhibited healthy breath profiles as well as SARS-CoV-2 RT-qPCR test results. CONCLUSIONS: In all, the use of olfactory technologies in communities with high transmission rates as well as in resource-limited settings where targeted sampling is not viable represents a practical COVID-19 screening approach capable of promptly identifying COVID-19 suspect patients and providing useful epidemiological information to guide community health strategies in the context of COVID-19.
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COVID-19 , SARS-CoV-2 , Humanos , Programas de Rastreamento , Sensibilidade e Especificidade , TecnologiaRESUMO
The storage and acquisition of human tissue samples have always been present in medicine for centuries; however, biobanks activity has only recently appeared as such. The aim of this review is to give a global perspective of the positioning of biobanks, particularly an overview of what happens in Mexico. One of the main challenges of our country is to establish concerted actions, in wich biobank networks will be developed and have the opportunity to share and create new science, and to build bridges to facilitate improved practices and standardization of biobanks.
El almacenamiento y la adquisición de muestras de tejido humano han estado presentes en la medicina desde hace siglos, no obstante, la aparición de los biobancos como tales es reciente. El objetivo de esta revisión es dar una perspectiva global del posicionamiento de los biobancos, en particular de lo que ocurre en México. Uno de los principales retos en nuestro país es establecer acciones concertadas con las que se desarrollen redes de biobancos, para tener la oportunidad de compartir y construir la nueva ciencia, así como para construir puentes que faciliten la mejora y la estandarización en las prácticas de los establecimientos.