RESUMO
Background: Limbal melanoma has been diagnosed in dogs and due to progression may cause vision loss and eyeballremoval. Definitive diagnosis is made through histopathological examination. Therapeutic options include full thicknessresection and repair by homologous corneal tissue, synthetic graft material, and enucleation. In this report, we describe acase of limbal melanocitoma in a dog that has been treated successfully with fresh homologous corneoscleral graft.Case: A 5-year-old female Labrador was referred to the Ophthalmology Veterinary Section of the Federal University ofRio Grande do Sul (UFRGS), Porto Alegre, Brazil, with a history of a pigmented mass located on the left eye. Ophthalmicexamination revealed a pigmented mass located at the left temporal limbus with corneal involvement. Surgical excisionfollowed by reconstruction using fresh homologous corneoscleral was recommended. The patient was premedicated withacepromazine (0.05 mg/kg, IM) and meperidine (20 mg/kg, IM). Anaesthesia was induced with propofol (10 mg/kg, IV)and maintained with isoflurane. Atracurium (0.2 mg/kg, IV) was administered to maintain a central eye position. The massand a free margin were removed by full-thickness corneoscleral resection. A corneoscleral graft was harvested from a dogthat had been euthanised for reasons unrelated to this study and sutured with 9-0 polyglactin 910 using a simple interruptedpattern. The mass was immediately fixed in 10% neutral buffered formalin and submitted for histological sectioning androutine staining. Based on the histopathological analysis it was confirmed limbal melanocytoma. Postoperative treatmentconsisted...(AU)
Assuntos
Animais , Feminino , Cães , Melanócitos/patologia , Melanoma/veterinária , Sistema Límbico/patologia , Transplante de Córnea/veterinária , Enucleação Ocular/veterináriaRESUMO
Background: Limbal melanoma has been diagnosed in dogs and due to progression may cause vision loss and eyeballremoval. Definitive diagnosis is made through histopathological examination. Therapeutic options include full thicknessresection and repair by homologous corneal tissue, synthetic graft material, and enucleation. In this report, we describe acase of limbal melanocitoma in a dog that has been treated successfully with fresh homologous corneoscleral graft.Case: A 5-year-old female Labrador was referred to the Ophthalmology Veterinary Section of the Federal University ofRio Grande do Sul (UFRGS), Porto Alegre, Brazil, with a history of a pigmented mass located on the left eye. Ophthalmicexamination revealed a pigmented mass located at the left temporal limbus with corneal involvement. Surgical excisionfollowed by reconstruction using fresh homologous corneoscleral was recommended. The patient was premedicated withacepromazine (0.05 mg/kg, IM) and meperidine (20 mg/kg, IM). Anaesthesia was induced with propofol (10 mg/kg, IV)and maintained with isoflurane. Atracurium (0.2 mg/kg, IV) was administered to maintain a central eye position. The massand a free margin were removed by full-thickness corneoscleral resection. A corneoscleral graft was harvested from a dogthat had been euthanised for reasons unrelated to this study and sutured with 9-0 polyglactin 910 using a simple interruptedpattern. The mass was immediately fixed in 10% neutral buffered formalin and submitted for histological sectioning androutine staining. Based on the histopathological analysis it was confirmed limbal melanocytoma. Postoperative treatmentconsisted...
Assuntos
Feminino , Animais , Cães , Enucleação Ocular/veterinária , Melanoma/veterinária , Melanócitos/patologia , Sistema Límbico/patologia , Transplante de Córnea/veterináriaRESUMO
The objective of this study was evaluate the maintenance of the corneal endothelium of horses in cold EUSOL-C® preservation medium over different periods (seven and 14 days) using scanning electron microscopy. A total of 20 pairs of eyes from horses were analysed. The corneas were divided into four groups of 10 corneas each (G1, G2, G3 and G4): G1 - the samples were kept in the preservation medium for seven days; G3 - the samples were kept in the preservation medium for for 14 days; G2 and G4 were formed by the control corneal buttons of G1 and G3, respectively. The average cell loss observed in G1 was 7.62%, in G2 it was 7.04%, in G3 9.12% and in G4 7.16%. No statistically significant differences were observed between the four groups. It was concluded that the Eusol-C® hypothermic preservation medium provided satisfactory preservation of the corneal endothelium in equine species for up to 14 days.
Objetivou-se avaliar a manutenção do endotélio da córnea de equinos em meio de preservação a frio EUSOL-C® em diferentes períodos de acondicionamento (sete e 14 dias) utilizando microscopia eletrônica de varredura. Foram avaliados 40 bulbos oculares de 20 equinos. Os bulbos oculares foram divididos em quatro grupos (G1, G2, G3 e G4), nos quais: G1 foi composto por 10 botões corneais acondicionados em meio de preservação para córnea Eusol-C® durante sete dias; o G3 foi formado por 10 botões corneais acondicionados em meio de preservação para córnea Eusol-C® durante 14 dias. O G2 e G4 foram formados pelas córneas controle armazenadas imediatamente em glutaraldeído. A média da perda celular observada no G1 foi de 7,62%, no G2 foi de 7,04 %, no G3 foi de 9,12% e o no G4 foi de 7,16%. Não foram observadas diferenças estatisticamente significativas entre os quatro grupos. Foi possível concluir que o meio de preservação hipotérmico Eusol-C® proporcionou de forma satisfatória a preservação do endotélio da córnea na espécie equina durante o período de até 14 dias.
Assuntos
Animais , Cavalos , Criopreservação/veterinária , Soluções para Preservação de Órgãos , Transplante de Córnea/veterinária , EndotélioRESUMO
The objective of this study was evaluate the maintenance of the corneal endothelium of horses in cold EUSOL-C® preservation medium over different periods (seven and 14 days) using scanning electron microscopy. A total of 20 pairs of eyes from horses were analysed. The corneas were divided into four groups of 10 corneas each (G1, G2, G3 and G4): G1 - the samples were kept in the preservation medium for seven days; G3 - the samples were kept in the preservation medium for for 14 days; G2 and G4 were formed by the control corneal buttons of G1 and G3, respectively. The average cell loss observed in G1 was 7.62%, in G2 it was 7.04%, in G3 9.12% and in G4 7.16%. No statistically significant differences were observed between the four groups. It was concluded that the Eusol-C® hypothermic preservation medium provided satisfactory preservation of the corneal endothelium in equine species for up to 14 days.(AU)
Objetivou-se avaliar a manutenção do endotélio da córnea de equinos em meio de preservação a frio EUSOL-C® em diferentes períodos de acondicionamento (sete e 14 dias) utilizando microscopia eletrônica de varredura. Foram avaliados 40 bulbos oculares de 20 equinos. Os bulbos oculares foram divididos em quatro grupos (G1, G2, G3 e G4), nos quais: G1 foi composto por 10 botões corneais acondicionados em meio de preservação para córnea Eusol-C® durante sete dias; o G3 foi formado por 10 botões corneais acondicionados em meio de preservação para córnea Eusol-C® durante 14 dias. O G2 e G4 foram formados pelas córneas controle armazenadas imediatamente em glutaraldeído. A média da perda celular observada no G1 foi de 7,62%, no G2 foi de 7,04 %, no G3 foi de 9,12% e o no G4 foi de 7,16%. Não foram observadas diferenças estatisticamente significativas entre os quatro grupos. Foi possível concluir que o meio de preservação hipotérmico Eusol-C® proporcionou de forma satisfatória a preservação do endotélio da córnea na espécie equina durante o período de até 14 dias.(AU)
Assuntos
Animais , Cavalos , Soluções para Preservação de Órgãos , Criopreservação/veterinária , Transplante de Córnea/veterinária , EndotélioRESUMO
The aim of this study was to investigate the acute effects of atracurium besylate on cellular damage in corneal endothelium of chickens. Twenty healthy chicken eyes were assigned to the following groups: Group 1 (G1), experimental group (n=10); and Group 2 (G2), control (n=10). Excised corneoscleral buttons were immediately placed on glass microscopy slides with endothelial region faced up. Corneal endothelium of eyes in G1 were covered with AB (0.2mL, 10mg/mL) for 3 min and then rinsed with balanced salt solution (BSS), while the corneal endothelium of eyes in G2 were covered with BBS for 3 min. Corneas from both groups were stained with alizarin red/trypan blue and visualized by light microscopy. Ten random photographs were taken from each cornea. The area of cellular damage was measured by software in all samples and cell loss of each group was averaged and compared. Endothelial area of denudation and Descemet's membrane exposure were higher in G1 than G2. In conclusion, atracurium besylate induced an acute damage on corneal endothelium of chickens.(AU)'
Objetivou-se avaliar os efeitos agudos do besilato de atracúrio sobre o endotélio corneano de galinhas. Vinte olhos saudáveis de galinhas foram aleatoriamente separados em dois grupos com 10 olhos cada, sendo G1 o grupo controle e G2 o grupo tratamento. Imediatamente após a excisão dos botões corneoesclerais estes foram colocados em lâminas de microscopia de vidro com o lado endotelial voltado para cima. No Grupo 1, o endotélio corneano foi recoberto com 0,2ml de besilato de atracúrio (10mg/ml) durante 3 minutos e depois lavado com solução salina balanceada. No Grupo 2, o endotélio corneano foi recoberto apenas com solução salina balanceada durante 3 min. As córneas de ambos os grupos foram coradas com vermelho de alizarina e azul de tripano e visualizadas com microscópio óptico. Foram obtidas dez fotografias aleatórias de cada amostra. As imagens foram analisadas e com auxílio de um software as áreas com ausência de células endoteliais calculadas. A perda celular endotelial foi significativamente maior no grupo tratamento comparativamente ao grupo controle. Com base nos resultados apresentados foi possível concluir que o besilato de atracúrio induziu dano agudo nas células do endotélio da córnea de galinhas.(AU)
Assuntos
Animais , Atracúrio/efeitos adversos , Endotélio Corneano/patologia , Midríase/veterinária , Galinhas , Perda de Células Endoteliais da Córnea/veterináriaRESUMO
The aim of this study was to investigate the acute effects of atracurium besylate on cellular damage in corneal endothelium of chickens. Twenty healthy chicken eyes were assigned to the following groups: Group 1 (G1), experimental group (n=10); and Group 2 (G2), control (n=10). Excised corneoscleral buttons were immediately placed on glass microscopy slides with endothelial region faced up. Corneal endothelium of eyes in G1 were covered with AB (0.2mL, 10mg/mL) for 3 min and then rinsed with balanced salt solution (BSS), while the corneal endothelium of eyes in G2 were covered with BBS for 3 min. Corneas from both groups were stained with alizarin red/trypan blue and visualized by light microscopy. Ten random photographs were taken from each cornea. The area of cellular damage was measured by software in all samples and cell loss of each group was averaged and compared. Endothelial area of denudation and Descemet's membrane exposure were higher in G1 than G2. In conclusion, atracurium besylate induced an acute damage on corneal endothelium of chickens.(AU)'
Objetivou-se avaliar os efeitos agudos do besilato de atracúrio sobre o endotélio corneano de galinhas. Vinte olhos saudáveis de galinhas foram aleatoriamente separados em dois grupos com 10 olhos cada, sendo G1 o grupo controle e G2 o grupo tratamento. Imediatamente após a excisão dos botões corneoesclerais estes foram colocados em lâminas de microscopia de vidro com o lado endotelial voltado para cima. No Grupo 1, o endotélio corneano foi recoberto com 0,2ml de besilato de atracúrio (10mg/ml) durante 3 minutos e depois lavado com solução salina balanceada. No Grupo 2, o endotélio corneano foi recoberto apenas com solução salina balanceada durante 3 min. As córneas de ambos os grupos foram coradas com vermelho de alizarina e azul de tripano e visualizadas com microscópio óptico. Foram obtidas dez fotografias aleatórias de cada amostra. As imagens foram analisadas e com auxílio de um software as áreas com ausência de células endoteliais calculadas. A perda celular endotelial foi significativamente maior no grupo tratamento comparativamente ao grupo controle. Com base nos resultados apresentados foi possível concluir que o besilato de atracúrio induziu dano agudo nas células do endotélio da córnea de galinhas.(AU)
Assuntos
Animais , Atracúrio/efeitos adversos , Endotélio Corneano/patologia , Midríase/veterinária , Galinhas , Perda de Células Endoteliais da Córnea/veterináriaRESUMO
Background: Ocular melanoma is very rare compared to cutaneous melanoma in horses. Definitive diagnosis is made through histopathological examination and treatment options include surgical excision associated with cryotherapy, radiation therapy, and chemotherapy. In this report, we describe a case of conjunctival melanoma in a horse that has been treated successfully with surgical excision associated with cryotherapy.Case: A 15-year-old male Percheron male was referred to the Ophthalmology Veterinary Section of the Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, Brazil, with a history of a pigmented mass located on the lower eyelid of the left eye. Ophthalmologic examination revealed ocular discomfort, secretion and a pigmented mass in the left inferior bulbar conjunctiva. The dermatological examination revealed other melanomas in the perineal region. Complete blood count and serum chemistry profile were within normal ranges and prior to surgery the horse was treated with flunixin meglumine (1.1 mg/kg, IV, q 12 h). Sedation was performed with xylazine (0.4 mg/kg, IV) and detomidine hydrochloride (0.01 mg/kg, IV) and then the animal was placed in a retention trunk. The conjunctival mass was resected with a margin of safety. Liquid nitrogen was applied to the tumor site and the adjacent conjunctiva with a copper cryoprobe with one unit of liquid nitrogen. Histopathological examination revealed neoplastic cells containing pigmented melanocytes in the conjunctival submucosa, confirming the diagnosis of conjunctival melanoma. Postoperative treatment was performed with flunixin meglumine (1.1 mg/kg, IV, q 12 h) for 3 days and topical ophthalmic ointment containing neomycin, polymyxin B sulfate and dexamethasone twice daily for one week. Seven days after surgery, the lesion was healed. The patient was followed for 24 months after excision and there was no evidence of recurrence.[...](AU)
Assuntos
Animais , Cavalos , Melanoma/veterinária , Melanoma/cirurgia , Neoplasias Oculares/veterinária , Criocirurgia/veterináriaRESUMO
Background: Ocular melanoma is very rare compared to cutaneous melanoma in horses. Definitive diagnosis is made through histopathological examination and treatment options include surgical excision associated with cryotherapy, radiation therapy, and chemotherapy. In this report, we describe a case of conjunctival melanoma in a horse that has been treated successfully with surgical excision associated with cryotherapy.Case: A 15-year-old male Percheron male was referred to the Ophthalmology Veterinary Section of the Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, Brazil, with a history of a pigmented mass located on the lower eyelid of the left eye. Ophthalmologic examination revealed ocular discomfort, secretion and a pigmented mass in the left inferior bulbar conjunctiva. The dermatological examination revealed other melanomas in the perineal region. Complete blood count and serum chemistry profile were within normal ranges and prior to surgery the horse was treated with flunixin meglumine (1.1 mg/kg, IV, q 12 h). Sedation was performed with xylazine (0.4 mg/kg, IV) and detomidine hydrochloride (0.01 mg/kg, IV) and then the animal was placed in a retention trunk. The conjunctival mass was resected with a margin of safety. Liquid nitrogen was applied to the tumor site and the adjacent conjunctiva with a copper cryoprobe with one unit of liquid nitrogen. Histopathological examination revealed neoplastic cells containing pigmented melanocytes in the conjunctival submucosa, confirming the diagnosis of conjunctival melanoma. Postoperative treatment was performed with flunixin meglumine (1.1 mg/kg, IV, q 12 h) for 3 days and topical ophthalmic ointment containing neomycin, polymyxin B sulfate and dexamethasone twice daily for one week. Seven days after surgery, the lesion was healed. The patient was followed for 24 months after excision and there was no evidence of recurrence.[...]
Assuntos
Animais , Cavalos , Melanoma/cirurgia , Melanoma/veterinária , Neoplasias Oculares/veterinária , Criocirurgia/veterináriaRESUMO
The goal of this study was to describe the normal values of corneal endothelial cell density and hexagonality in healthy rabbits eyes using contact specular microscopy. Both eyes of 18 rabbits, males and females, of different ages were evaluated. The eyes were divided into three groups of 12 each according to the age range of the animals: G1 (6 months old), G2 (12 months old), and G3 (48 months old). Parameters studied included mean endothelial cell density and hexagonality. For Group I, the mean cell density was 2307 cells per mm2. For GII, the mean cell density was 1895 cells per mm2. For G III, the mean cell density was 1818 cells per mm2. Cell density decreased significantly with aging (P 0.001) among the three groups. Pleomorphism in the rabbits of GI was 74.33±10.08. In the rabbits of GII it was 71.83±11.38. In the rabbits of GIII it was 64.02±28.80. Significant differences (P 0.001) were evident between GI and GIII and GII and GIII but not between Groups I and II. The results showed a decrease in number of cells and hexagonality with aging. Corneal endothelium of rabbits suffers changes due to advancing age both in terms of cell density and morphology.
Objetivou-se avaliar a densidade celular e a hexagonalidade do endotélio da cَrnea de coelhos saudلveis usando um microscَpio especular de contato. Os dois olhos de 18 coelhos, machos ou fêmeas, de diferentes idades foram avaliados. Os olhos foram separados em três grupos de 12 olhos cada em funçمo da idade: G1 (6 meses de idade), G2 (12 meses de idade) e G3 (48 meses de idade). Os parâmetros estudados incluيram a densidade celular e a hexagonalidade. Para o Grupo I, a densidade celular média foi de 2307 células por mm2. Para GII, a densidade celular média foi de 1895 células por mm2. Para G III, a densidade celular média foi de 1818 células por mm2. A densidade celular diminuiu significativamente com o envelhecimento (P 0,001) entre os três grupos. O pleomorfismo nos coelhos de GI foi de 74,33±10,08. Nos coelhos do GII foi 71,83±11,38. Nos coelhos de GIII foi de 64,02±28,80. Diferenças significativas (P 0,001) foram evidentes entre GI e GIII e GII e GIII, mas nمo entre os Grupos I e II. Os resultados revelaram diminuiçمo da densidade celular e da hexagonalidade com o avanço da idade. O endotélio da cَrnea de coelhos sofre alteraçُes devido ao avanço da idade tanto na densidade celular e quanto na morfologia.
Assuntos
Coelhos , Coelhos , Endotélio Corneano , LagomorphaRESUMO
The goal of this study was to describe the normal values of corneal endothelial cell density and hexagonality in healthy rabbits eyes using contact specular microscopy. Both eyes of 18 rabbits, males and females, of different ages were evaluated. The eyes were divided into three groups of 12 each according to the age range of the animals: G1 (6 months old), G2 (12 months old), and G3 (48 months old). Parameters studied included mean endothelial cell density and hexagonality. For Group I, the mean cell density was 2307 cells per mm2. For GII, the mean cell density was 1895 cells per mm2. For G III, the mean cell density was 1818 cells per mm2. Cell density decreased significantly with aging (P 0.001) among the three groups. Pleomorphism in the rabbits of GI was 74.33±10.08. In the rabbits of GII it was 71.83±11.38. In the rabbits of GIII it was 64.02±28.80. Significant differences (P 0.001) were evident between GI and GIII and GII and GIII but not between Groups I and II. The results showed a decrease in number of cells and hexagonality with aging. Corneal endothelium of rabbits suffers changes due to advancing age both in terms of cell density and morphology.(AU)
Objetivou-se avaliar a densidade celular e a hexagonalidade do endotélio da cَrnea de coelhos saudلveis usando um microscَpio especular de contato. Os dois olhos de 18 coelhos, machos ou fêmeas, de diferentes idades foram avaliados. Os olhos foram separados em três grupos de 12 olhos cada em funçمo da idade: G1 (6 meses de idade), G2 (12 meses de idade) e G3 (48 meses de idade). Os parâmetros estudados incluيram a densidade celular e a hexagonalidade. Para o Grupo I, a densidade celular média foi de 2307 células por mm2. Para GII, a densidade celular média foi de 1895 células por mm2. Para G III, a densidade celular média foi de 1818 células por mm2. A densidade celular diminuiu significativamente com o envelhecimento (P 0,001) entre os três grupos. O pleomorfismo nos coelhos de GI foi de 74,33±10,08. Nos coelhos do GII foi 71,83±11,38. Nos coelhos de GIII foi de 64,02±28,80. Diferenças significativas (P 0,001) foram evidentes entre GI e GIII e GII e GIII, mas nمo entre os Grupos I e II. Os resultados revelaram diminuiçمo da densidade celular e da hexagonalidade com o avanço da idade. O endotélio da cَrnea de coelhos sofre alteraçُes devido ao avanço da idade tanto na densidade celular e quanto na morfologia.(AU)
Assuntos
Coelhos , Coelhos , Endotélio Corneano , LagomorphaRESUMO
ABSTRACT: The goal of this study was to describe the normal values of corneal endothelial cell density and hexagonality in healthy rabbits' eyes using contact specular microscopy. Both eyes of 18 rabbits, males and females, of different ages were evaluated. The eyes were divided into three groups of 12 each according to the age range of the animals: G1 (6 months old), G2 (12 months old), and G3 (48 months old). Parameters studied included mean endothelial cell density and hexagonality. For Group I, the mean cell density was 2307 cells per mm2. For GII, the mean cell density was 1895 cells per mm2. For G III, the mean cell density was 1818 cells per mm2. Cell density decreased significantly with aging (P<0.001) among the three groups. Pleomorphism in the rabbits of GI was 74.33±10.08. In the rabbits of GII it was 71.83±11.38. In the rabbits of GIII it was 64.02±28.80. Significant differences (P<0.001) were evident between GI and GIII and GII and GIII but not between Groups I and II. The results showed a decrease in number of cells and hexagonality with aging. Corneal endothelium of rabbits suffers changes due to advancing age both in terms of cell density and morphology.
RESUMO: Objetivou-se avaliar a densidade celular e a hexagonalidade do endotélio da córnea de coelhos saudáveis usando um microscópio especular de contato. Os dois olhos de 18 coelhos, machos ou fêmeas, de diferentes idades foram avaliados. Os olhos foram separados em três grupos de 12 olhos cada em função da idade: G1 (6 meses de idade), G2 (12 meses de idade) e G3 (48 meses de idade). Os parâmetros estudados incluíram a densidade celular e a hexagonalidade. Para o Grupo I, a densidade celular média foi de 2307 células por mm2. Para GII, a densidade celular média foi de 1895 células por mm2. Para G III, a densidade celular média foi de 1818 células por mm2. A densidade celular diminuiu significativamente com o envelhecimento (P<0,001) entre os três grupos. O pleomorfismo nos coelhos de GI foi de 74,33±10,08. Nos coelhos do GII foi 71,83±11,38. Nos coelhos de GIII foi de 64,02±28,80. Diferenças significativas (P<0,001) foram evidentes entre GI e GIII e GII e GIII, mas não entre os Grupos I e II. Os resultados revelaram diminuição da densidade celular e da hexagonalidade com o avanço da idade. O endotélio da córnea de coelhos sofre alterações devido ao avanço da idade tanto na densidade celular e quanto na morfologia.
RESUMO
Background: Indolent corneal ulcers have been described as superficial ulcers with an associated rim of loose peripheral epithelium Treatment for indolent ulcers include debridement, grid keratotomy, multiple punctate keratotomy, third eyelid flaps, application of cyanoacrylate tissue adhesives, superficial keratectomy, and a debridement with a diamond burr. Case: A 2-month-old female American Quarter Horse was referred to the Ophthalmology Veterinary Section of the Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, Brazil, presenting epiphora and blepharospasm. A local veterinarian doctor had prescribed broad spectrum topical antibiotic and anti-inflammatory drops, although there was no positive response to the treatment for the past two weeks. Ophthalmic examination reveals and moderate discomfort in the left eye, epiphora, and mild corneal edema in the area of the defect. Slit lamp biomicroscopy revealed a superficial corneal ulcer with about 6 mm. Corneal epithelium did not adhere to underlying corneal stroma. The remainder of the ophthalmic examination of the left eye was unremarkable. The diagnosis of a corneal ulcer was made based on these clinical signs and fluorescein staining of the cornea where the stain dissects under the unattached epithelial lip. A handheld battery-operated motorized diamond burr, with a 3.5 mm medium grit tip, was utilized to remove the epithelium. The medical treatment included tobramycin eye drops, and flurbiprofen sodium ophthalmic solution, being applied six times daily, after the procedure, during two weeks, and atropine sulphate 1% was applied once a day, during three days. The foal was hospitalized until healing the corneal ulcer. For two weeks, the foal was assessed daily, and, after that, follow-up visits were scheduled weekly for four months. Healing was defined as the point at which the cornea no longer retained fluorescein. [ ](AU)
Assuntos
Animais , Úlcera da Córnea/terapia , Úlcera da Córnea/veterinária , Cavalos , Instrumentos Cirúrgicos/veterinária , Infecções Oculares/veterináriaRESUMO
Background: Indolent corneal ulcers have been described as superficial ulcers with an associated rim of loose peripheral epithelium Treatment for indolent ulcers include debridement, grid keratotomy, multiple punctate keratotomy, third eyelid flaps, application of cyanoacrylate tissue adhesives, superficial keratectomy, and a debridement with a diamond burr. Case: A 2-month-old female American Quarter Horse was referred to the Ophthalmology Veterinary Section of the Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, Brazil, presenting epiphora and blepharospasm. A local veterinarian doctor had prescribed broad spectrum topical antibiotic and anti-inflammatory drops, although there was no positive response to the treatment for the past two weeks. Ophthalmic examination reveals and moderate discomfort in the left eye, epiphora, and mild corneal edema in the area of the defect. Slit lamp biomicroscopy revealed a superficial corneal ulcer with about 6 mm. Corneal epithelium did not adhere to underlying corneal stroma. The remainder of the ophthalmic examination of the left eye was unremarkable. The diagnosis of a corneal ulcer was made based on these clinical signs and fluorescein staining of the cornea where the stain dissects under the unattached epithelial lip. A handheld battery-operated motorized diamond burr, with a 3.5 mm medium grit tip, was utilized to remove the epithelium. The medical treatment included tobramycin eye drops, and flurbiprofen sodium ophthalmic solution, being applied six times daily, after the procedure, during two weeks, and atropine sulphate 1% was applied once a day, during three days. The foal was hospitalized until healing the corneal ulcer. For two weeks, the foal was assessed daily, and, after that, follow-up visits were scheduled weekly for four months. Healing was defined as the point at which the cornea no longer retained fluorescein. [ ]
Assuntos
Animais , Cavalos , Instrumentos Cirúrgicos/veterinária , Úlcera da Córnea/terapia , Úlcera da Córnea/veterinária , Infecções Oculares/veterináriaRESUMO
The aim was to investigate the ultrastructural changes in the corneal endothelium of pigs induced by intracameral 0.05 % brilliant blue. Twenty swine corneas were separated into two groups, the right eye bulbs (control group) and the left eye bulbs (experimental group) of the same animal. All the eye bulbs were evaluated with specular microscopy. The cornea of the right eye bulbs was excised and in the left eye bulbs 0.2ml of 0.05% brilliant blue vital dye (OPTH-blue±) was injected into the anterior chamber, where it remained for one minute. Then the anterior chamber was cleaned with a balanced salt solution injection and the cornea was excised too. All the corneas were evaluated by scanning electron microscopy to evaluate the changes on the endothelium caused by the brilliant blue dye. There were no significant differences between the right corneal endothelium cells and the left corneal endothelium cells with scanning electron microscopy after intracameral use of 0.05 % brilliant blue dye. The 0.05% brilliant blue dye concentration did not cause deleterious effects for the swine corneal endothelium after intracameral use and can be a choice for safe staining of the anterior capsule of the lens in cataract surgery.(AU)
Com o objetivo de avaliar as alterações ultraestruturais no endotélio corneano suíno induzidas pela aplicação intracameral do corante azul brilhante 0,05 %, vinte córneas suínas foram separadas em dois grupos: olhos direitos (grupo controle) e olhos esquerdos (grupo experimental). Inicialmente todos os bulbos oculares foram avaliados por microscopia especular. As córneas dos bulbos oculares do grupo controle foram excisadas, enquanto nos bulbos oculares do grupo experimental foram injetados 0,2ml do corante vital azul brilhante 0,05% na câmara anterior e mantido por um minuto. Após esse período a câmara anterior foi lavada com solução salina balanceada e as córneas foram excisadas. Todas as córneas excisadas foram avaliadas por microscopia eletrônica de varredura para verificar a ocorrência possíveis alterações no endotélio corneano causadas pelo corante. Não foram observadas diferenças ultraestruturais durante a avaliação endotelial por microscopia eletrônica de varredura das córneas do grupo controle e experimental após a aplicação do azul brilhante 0,05 % na câmara anterior. O corante azul brilhante na concentração 0,05% não causou danos endoteliais após seu uso intracameral em suínos e pode ser uma alternativa segura para a coloração da cápsula anterior da lente na cirurgia de catarata.(AU)
Assuntos
Animais , Suínos , Endotélio Corneano , Corantes/administração & dosagem , Corantes/toxicidade , Microscopia Eletrônica de Varredura/veterináriaRESUMO
The aim was to investigate the ultrastructural changes in the corneal endothelium of pigs induced by intracameral 0.05% brilliant blue. Twenty swine corneas were separated into two groups, the right eye bulbs (control group) and the left eye bulbs (experimental group) of the same animal. All the eye bulbs were evaluated with specular microscopy. The cornea of the right eye bulbs was excised and in the left eye bulbs 0.2ml of 0.05% brilliant blue vital dye (OPTH-blue±) was injected into the anterior chamber, where it remained for one minute. Then the anterior chamber was cleaned with a balanced salt solution injection and the cornea was excised too. All the corneas were evaluated by scanning electron microscopy to evaluate the changes on the endothelium caused by the brilliant blue dye. There were no significant differences between the right corneal endothelium cells and the left corneal endothelium cells with scanning electron microscopy after intracameral use of 0.05% brilliant blue dye. The 0.05% brilliant blue dye concentration did not cause deleterious effects for the swine corneal endothelium after intracameral use and can be a choice for safe staining of the anterior capsule of the lens in cataract surgery.(AU)
Com o objetivo de avaliar as alterações ultraestruturais no endotélio corneano suíno induzidas pela aplicação intracameral do corante azul brilhante 0,05%, vinte córneas suínas foram separadas em dois grupos: olhos direitos (grupo controle) e olhos esquerdos (grupo experimental). Inicialmente todos os bulbos oculares foram avaliados por microscopia especular. As córneas dos bulbos oculares do grupo controle foram excisadas, enquanto nos bulbos oculares do grupo experimental foram injetados 0,2ml do corante vital azul brilhante 0,05% na câmara anterior e mantido por um minuto. Após esse período a câmara anterior foi lavada com solução salina balanceada e as córneas foram excisadas. Todas as córneas excisadas foram avaliadas por microscopia eletrônica de varredura para verificar a ocorrência possíveis alterações no endotélio corneano causadas pelo corante. Não foram observadas diferenças ultraestruturais durante a avaliação endotelial por microscopia eletrônica de varredura das córneas do grupo controle e experimental após a aplicação do azul brilhante 0,05% na câmara anterior. O corante azul brilhante na concentração 0,05% não causou danos endoteliais após seu uso intracameral em suínos e pode ser uma alternativa segura para a coloração da cápsula anterior da lente na cirurgia de catarata.(AU)
Assuntos
Animais , Corantes/administração & dosagem , Corantes/toxicidade , Endotélio Corneano , Suínos , Microscopia Eletrônica de Varredura/veterináriaRESUMO
Background: Eyelid apocrine hidrocystoma (AH) is a benign cystic lesion originating from the glands of Moll. These eyelid lesions are rare in cats and there are few cases reported in the literature. The aetiopathogenesis of AH is unknown and the lesion has been interpreted either as proliferative adenomatous tumour or as retention cyst. Definitive diagnosis requires biopsy with microscopic examination. Treatment options for eyelid AH include surgical excision, drainage through aspiration, cryosurgery with liquid nitrogen and chemical ablation. The aim is to report a case of bilateral multiple AH of the eyelids in a cat that was successfully treated with surgical excision. Case: A 5-year-old male Himalayan cat was referred to the Ophthalmology Section in the Veterinary Clinics Hospital of the Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil with a history of pigmented masses in both eyes that had been progressively enlarging over a 1-year period. Ophthalmic examination revealed two cysts on the medial canthus at the eyelid margin in both eyes. In the left eye was observed mild blepharospasm and lacrimation. Cysts were soft, smooth, round and filled with a dark brownish pigment. The remainder of the ophthalmic examination was unremarkable. Surgical excision of the cysts was indicated with a safety margin. The surgical procedure was performed under [...]
Assuntos
Animais , Gatos , Glândulas Apócrinas/veterinária , Hidrocistoma/cirurgia , Hidrocistoma/etiologia , Hidrocistoma/veterinária , Adenoma de Glândula Sudorípara/veterináriaRESUMO
Background: Eyelid apocrine hidrocystoma (AH) is a benign cystic lesion originating from the glands of Moll. These eyelid lesions are rare in cats and there are few cases reported in the literature. The aetiopathogenesis of AH is unknown and the lesion has been interpreted either as proliferative adenomatous tumour or as retention cyst. Definitive diagnosis requires biopsy with microscopic examination. Treatment options for eyelid AH include surgical excision, drainage through aspiration, cryosurgery with liquid nitrogen and chemical ablation. The aim is to report a case of bilateral multiple AH of the eyelids in a cat that was successfully treated with surgical excision. Case: A 5-year-old male Himalayan cat was referred to the Ophthalmology Section in the Veterinary Clinics Hospital of the Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil with a history of pigmented masses in both eyes that had been progressively enlarging over a 1-year period. Ophthalmic examination revealed two cysts on the medial canthus at the eyelid margin in both eyes. In the left eye was observed mild blepharospasm and lacrimation. Cysts were soft, smooth, round and filled with a dark brownish pigment. The remainder of the ophthalmic examination was unremarkable. Surgical excision of the cysts was indicated with a safety margin. The surgical procedure was performed under [...](AU)
Assuntos
Animais , Gatos , Hidrocistoma/veterinária , Hidrocistoma/etiologia , Hidrocistoma/cirurgia , Glândulas Apócrinas/veterinária , Adenoma de Glândula Sudorípara/veterináriaRESUMO
Background: Hemangiosarcoma (HSA) is a malignant tumor of vascular endothelial origin. Primary neoplasms are extremely rare in the third eyelid of dogs. Neoplasia affecting ocular surface has been associated with increased exposure to solar radiation. Treatment reported for dogs with conjunctival neoplasia is surgical excision. Definitive diagnosis is confirmed in the histophatological examination. In this report, we describe a case of HSA of the third eyelid in a dog that was successfully treated with surgical excision.Case: A 5-year-old female Boxer dog was referred to the Ophthalmology Section of the Veterinary Clinics Hospital of the Federal University of Rio Grande do Sul (UFRGS), presenting a red mass in the third eyelid of the right eye. Physical examination it was normal except for the ocular disease. The dog had signs of ocular discomfort an epiphora. On ophthalmic examination a fixed, raised, red mass approximately 4 to 5 mm in diameter was identified on the anterior surface of the third eyelid in the right eye. Under general anesthesia, and using an operating microscope the mass was excised with a gross margin and submitted for histopathological analysis. Microscopic examination revealed a lesion composed of neoplastic mesenchymal cells forming irregular channels containing blood. These plump cells showed moderate anisocytosis and anisokaryosis with a low mitotic...
Assuntos
Animais , Cães , Hemangiossarcoma/cirurgia , Hemangiossarcoma/veterinária , Membrana Nictitante/cirurgia , Neoplasias da Túnica Conjuntiva/cirurgia , Neoplasias da Túnica Conjuntiva/veterináriaRESUMO
Background: Hemangiosarcoma (HSA) is a malignant tumor of vascular endothelial origin. Primary neoplasms are extremely rare in the third eyelid of dogs. Neoplasia affecting ocular surface has been associated with increased exposure to solar radiation. Treatment reported for dogs with conjunctival neoplasia is surgical excision. Definitive diagnosis is confirmed in the histophatological examination. In this report, we describe a case of HSA of the third eyelid in a dog that was successfully treated with surgical excision.Case: A 5-year-old female Boxer dog was referred to the Ophthalmology Section of the Veterinary Clinics Hospital of the Federal University of Rio Grande do Sul (UFRGS), presenting a red mass in the third eyelid of the right eye. Physical examination it was normal except for the ocular disease. The dog had signs of ocular discomfort an epiphora. On ophthalmic examination a fixed, raised, red mass approximately 4 to 5 mm in diameter was identified on the anterior surface of the third eyelid in the right eye. Under general anesthesia, and using an operating microscope the mass was excised with a gross margin and submitted for histopathological analysis. Microscopic examination revealed a lesion composed of neoplastic mesenchymal cells forming irregular channels containing blood. These plump cells showed moderate anisocytosis and anisokaryosis with a low mitotic...(AU)
Assuntos
Animais , Cães , Hemangiossarcoma/cirurgia , Hemangiossarcoma/veterinária , Membrana Nictitante/cirurgia , Neoplasias da Túnica Conjuntiva/cirurgia , Neoplasias da Túnica Conjuntiva/veterináriaRESUMO
INTRODUCTION: The purpose of this study was to measure cell density, average area, and morphology of corneal endothelium of chinchillas in different age groups. MATERIALS AND METHODS: The corneal endothelium was studied with a contact specular microscope. Thirty chinchillas (Chinchilla lanigera) were evaluated, and 30 male chinchillas were divided into three groups of 10 chinchillas each, by age: Group 1 (2-4-month-old), Group 2 (48-month-old), and Group 3 (10 years of age). Data are presented as endothelial cell density (ECD), average cell area, and pleomorphism. RESULTS: Endothelial cell density decreases and an increase in endothelial cell area and pleomorphism were observed with age, in the corneas of normal chinchillas. For Group 1 chinchillas, the mean cell density was 3.423 cells per mm(2) . The mean ECD for Group 2 chinchillas was 2.650 cells per mm(2) . The mean ECD for Group 3 chinchillas was 2.124 cells per mm(2) . The average area for Group 1 chinchillas was 350.5 µm(2) . The average area for Group 2 chinchillas was 442.15 µm(2) . The average area for Group 3 chinchillas was 583.64 µm(2) . The pleomorphism for Group 1 was 70.05%. The pleomorphism for Group 2 was 65.18%, and the pleomorphism for Group 3 was 62.28%. CONCLUSIONS: The results suggested that chinchilla corneal endothelium undergoes age-related changes. Moreover, with advancing age, the mean cell area increased and cell density decreased.