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Mem. Inst. Oswaldo Cruz ; 105(6): 786-791, Sept. 2010. ilus, graf, tab
Artigo em Inglês | LILACS | ID: lil-560663

RESUMO

Enteroinvasive Escherichia coli (EIEC) and Shigellaspp cause bacillary dysentery in humans by invading and multiplying within epithelial cells of the colonic mucosa. Although EIEC and Shigellashare many genetic and biochemical similarities, the illness caused by Shigellais more severe. Thus, genomic and structure-function molecular studies on the biological interactions of these invasive enterobacteria with eukaryotic cells have focused on Shigella rather than EIEC. Here we comparatively studied the interactions of EIEC and of Shigella flexneriwith cultured J774 macrophage-like cells. We evaluated several phenotypes: (i) bacterial escape from macrophages after phagocytosis, (ii) macrophage death induced by EIEC and S. flexneri, (iii) macrophage cytokine expression in response to infection and (iv) expression of plasmidial (pINV) virulence genes. The results showed thatS. flexneri caused macrophage killing earlier and more intensely than EIEC. Both pathogens induced significant macrophage production of TNF, IL-1 and IL-10 after 7 h of infection. Transcription levels of the gene invasion plasmid antigen-C were lower in EIEC than in S. flexneri throughout the course of the infection; this could explain the diminished virulence of EIEC compared to S. flexneri.


Assuntos
Humanos , Citocinas , Escherichia coli , Regulação Bacteriana da Expressão Gênica/imunologia , Macrófagos , Shigella flexneri , Fatores de Virulência/biossíntese , Morte Celular , ELISPOT , Escherichia coli , Genes Bacterianos , Regulação Bacteriana da Expressão Gênica , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Shigella flexneri , Fatores de Virulência
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