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Levamisole is a veterinary anthelmintic drug and a common adulterant of misused drugs. This study analyses the lethal, antinociceptive and haematological effects produced by acute or repeated levamisole administration by itself or combined with morphine. Independent groups of male Swiss Webster mice were i.p. injected with 100 mg/kg morphine, 31.6 mg/kg levamisole (lethal doses at 10%, LD10 ) or the same doses combined. Naloxone pretreatment (10 mg/kg, i.p.) prevented morphine-induced death, as did 2.5 mg/kg, i.p. mecamylamine with levamisole. Co-administration of levamisole and morphine (Lvm + Mor) increased lethality from 10% to 80%. This augmented effect was prevented by 30 mg/kg, i.p. naloxone and reduced with 10 mg/kg naloxone plus 2.5 mg/kg, i.p. mecamylamine. In independent groups of mice, 17.7 mg/kg, i.p. levamisole antagonized the acute morphine's antinociceptive effect evaluated in the tail-flick test. Repeated 17.7 mg/kg levamisole administration (2×/day/3 weeks) did not affect tolerance development to morphine (10 mg/kg, 3×/day/1 week). Blood samples obtained from mice repeatedly treated with levamisole showed leukopenia and neutropenia. Morphine also produced neutropenia, increased erythrocyte count and other related parameters (e.g. haemoglobin). Lvm + Mor had similar effects on leukocyte and neutrophil counts to those seen with levamisole only, but no erythrocyte-related alterations were evident. Blood chemistry analysis did not indicate liver damage but suggested some degree of electrolyte balance impairment. In conclusion, Lvm + Mor increased death risk, altered morphine-induced antinociceptive effects and produced haematologic abnormalities. The importance of studying combinations of drugs of abuse lies in the fact that drug users frequently combine drugs, which are commonly adulterated.

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Morphine promotes neuroinflammation after NOD-like receptor protein 3 (NLRP3) oligomerization in glial cells, but the capacity of other opioids to induce neuroinflammation and its relationship to the development of analgesic tolerance is unknown. We studied the effects of morphine and fentanyl on NLRP3 inflammasome activation in glial and neuronal cells in the dorsal raphe nucleus (DRN), a region involved in pain regulation. Male Wistar rats received i.p. injections of morphine (10 mg/kg) or fentanyl (0.1 mg/kg) 3 × daily for 7 days and were tested for nociception. Two hours after the last (19th) administration, we analyzed NLRP3 oligomerization, caspase-1 activation and gasdermin D-N (GSDMD-N) expression in microglia (CD11b positive cells), astrocytes (GFAP-positive cells) and neurons (NeuN-positive cells). Tolerance developed to both opioids, but only fentanyl produced hyperalgesia. Morphine and fentanyl activated NLRP3 inflammasome in astrocytes and serotonergic (TPH-2-positive) neurons, but fentanyl effects were more pronounced. Both opioids increased GFAP and CD11b immunoreactivity, caspase-1 and GSDMD activation, indicating pyroptotic cell death. The opioid receptor antagonist (-)-naloxone, but not the TLR4 receptor antagonist (+)-naloxone, prevented microglia activation and NLRP3 oligomerization. Only (+)-naloxone prevented astrocytes' activation. The anti-inflammatory agent minocycline and the NLRP3 inhibitor MCC950 delayed tolerance to morphine and fentanyl antinociception and prevented fentanyl-induced hyperalgesia. MCC950 also prevented opioid-induced NLRP3 oligomerization. In conclusion, morphine and fentanyl differentially induce cell-specific activation of NLRP3 inflammasome and pyroptosis in the DRN through TLR4 receptors in astrocytes and through opioid receptors in neurons, indicating that neuroinflammation is involved in opioid-induced analgesia and fentanyl-induced hyperalgesia after repeated administrations.

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Resumen En este trabajo se presenta una lista actualizada de los anfibios distribuidos en el departamento de Loreto, Perú, incluyendo información sobre el estado de conservación a nivel internacional y nacional, el endemismo y zonas con vacíos de información. Se utilizó literatura herpetológica, bases de datos y especímenes de la colección del Departamento de Herpetología del Museo de Historia Natural de la Universidad Nacional Mayor de San Marcos para la elaboración de esta lista. Se registraron un total de 232 especies, con los anuros (sapos y ranas) representando el 96% del total de anfibios en Loreto. Dieciocho especies son endémicas de Loreto mientras que la gran mayoría (208 especies) tienen una distribución altitudinal por debajo de los 500 m. Diez especies están en categorías de amenaza y cuatro son consideradas casi amenazadas según criterios internacionales, mientras que cuatro están consideradas como amenazadas y seis como casi amenazadas en el listado nacional. Se observa mayor vacío de información en áreas cercanas a las fronteras con Brasil y Colombia.

Abstract This paper presents an updated list of the amphibians occurring in the department of Loreto, Peru, including information on conservation status, endemic species and areas with information gaps. Herpetological literature, databases and the collection of the Department of Herpetology of the Natural History Museum of the National University of San Marcos were used to update the species list. A total of 232 species were recorded, with anurans (toads and frogs) representing 96% of amphibians in Loreto. eighteen species are endemic to Loreto while the vast majority (208 species) have an altitudinal distribution below 500 m. Ten species are in threatened categories and four are near threatened according to international criteria; while four are considered as threatened and six as near threatened according the national list. Mayor information gaps are observed in areas near the borders with Brazil and Colombia.

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BACKGROUND: A central aspect of professional identity is occupational commitment, which has a significant relationship with motivation and positive mental states in healthcare environments. However, it is not clear how occupational commitment is related to teaching practices in universities. AIM: To apply the Occupational Commitment Questionnaire (OCQ) to university teachers and relate its results with the Teaching Practices Questionnaire. MATERIAL AND METHODS: OCQ and Teaching Practices Questionnaire were answered by 319 university teachers from 13 Chilean regions, who were selected through a non-probabilistic volunteer sampling. Data collection was done after informed consent. An exploratory factor analysis was performed for OCQ. The internal consistency of each factor was calculated with Cronbach's Alpha. Spearman correlations were used to explore the relationship between the two questionnaires. RESULTS: Two factors were identified in OCQ, namely Teacher Commitment and Ethical-Professional Commitment. Cronbach's Alpha was 0.85 for both factors. A moderate direct relationship between teacher commitment, ethical-professional commitment, and the Teaching Practices Questionnaire was found. CONCLUSIONS: OCQ showed an adequate internal consistency, and it was directly related with teaching practices. Committed teachers had better teaching practices. This confirms the importance of teachers' values and perceptions on their professional performance.

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Background: A central aspect of professional identity is occupational commitment, which has a significant relationship with motivation and positive mental states in healthcare environments. However, it is not clear how occupational commitment is related to teaching practices in universities. Aim: To apply the Occupational Commitment Questionnaire (OCQ) to university teachers and relate its results with the Teaching Practices Questionnaire. Material and Methods: OCQ and Teaching Practices Questionnaire were answered by 319 university teachers from 13 Chilean regions, who were selected through a non-probabilistic volunteer sampling. Data collection was done after informed consent. An exploratory factor analysis was performed for OCQ. The internal consistency of each factor was calculated with Cronbach's Alpha. Spearman correlations were used to explore the relationship between the two questionnaires. Results: Two factors were identified in OCQ, namely Teacher Commitment and Ethical-Professional Commitment. Cronbach's Alpha was 0.85 for both factors. A moderate direct relationship between teacher commitment, ethical-professional commitment, and the Teaching Practices Questionnaire was found. Conclusions: OCQ showed an adequate internal consistency, and it was directly related with teaching practices. Committed teachers had better teaching practices. This confirms the importance of teachers' values and perceptions on their professional performance.

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Resumen En este estudio se investigó la dieta de la lagartija neotropical Liolaemus polystictus en la región Huancavelica en los Andes centrales del Perú. Los ítems de la dieta fueron identificados en los contenidos estomacales de 54 muestras. El porcentaje de contenido volumétrico vegetal fue mayor al animal (V% contenido animal= 22.97 y V% contenido vegetal= 77.03), siendo el contenido animal representado por los artrópodos y con diez ítems alimentarios. Considerando solo el contenido animal, los coleópteros fueron los más representativos (N%= 32.05, FO=59.26 y V%= 41.36). Ninguna categoría animal mostró valores de IRI altos para ser considerados fundamentales, asimismo la selección de presas no mostro diferencias significativas entre machos, hembras y juveniles, ni entre los contenidos volumétricos vegetales entre adultos y juveniles, pero sí en los contenidos volumétricos vegetales entre machos y hembras. Se encontraron diferencias significativas entre la longitud, ancho y volumen de la presa y el ancho de la cabeza (AC) en machos de L. polystictus. Finalmente, proponemos la categoría de herbívoro para la especie L. polystictus, lo cual elevaría a ocho las especies del grupo L. montanus que se alimentan principalmente de vegetación.

Abstract This study assessed the diet of the Neotropical lizard Liolaemus polystictus from Huancavelica region in the Peruvian central Andes. The diet composition was identified from 54 sample stomach contents. Percentage of vegetable content was higher than the animal one (V% animal content= 22.97 and V% vegetable content= 77.03), and arthropods were the highest animal content with ten foods items. Within the animal content, Coleoptera were the most representative (N%= 32.05, FO= 59.26 and V%= 41.36%). No animal category showed high IRI values to be considered fundamental. There were not significant differences on prey selection between males, females and juveniles, and on vegetal volumetric contents between adults and juveniles. However, vegetal volumetric contents were significantly different between males and females. Significant differences were found between prey length, width and volume and L. polystictus male head width (AC). Finally, we pro-pose L. polystictus to be considered an herbivorous species, which bring to eight the number of species in the L. montanus group that feed mainly on vegetation.

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This study presents a comparison of SO2 concentrations simulated by a dispersion model, using as input both emission data inventories of the Mexican petroleum company Petroleos Mexicanos and emissions estimated by the Ozone Monitoring Instrument on board of the Aura satellite, contrasting results with data measured by an on-ground sensor located in the city of Villahermosa, Tabasco, Mexico, during the month of February 2013. The emissions used came from both oil extraction facilities located in the Gulf of Mexico and oil installations located on land. The days selected to be modeled were those when the winds had a northern component, and there was an observable emission detected by the Aura satellite coming from the emission sources. Evaluation analysis of the results showed that satellite data overestimate SO2 concentration, while the inventories of Petroleos Mexicanos underestimate it. Good correlations (r, r2, and concordance index) were observed between simulated and measured concentrations when the winds had a high frequency of directions of northern component and not-so weak speeds. It is recommended that Mexican oil company calculates more precise and updated emission inventories, considering not only a higher sulfur content of the gas emitted to the atmosphere and its combustion process but also from other existing sources of SO2 emission in this region.

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Se revisa el estado taxonómico de Gastrotheca peruana usando métodos filogenéticos en base a secuencias de 16S rRNA. Los árboles de máxima verosimilitud y Bayesiano mostraron que las variantes génicas de G. peruana forman dos clados que no son hermanos. Uno de estos clados se distribuye en el norte de Perú, incluyendo un individuo procedente de la localidad típica de G. peruana dissimilis. El segundo clado está restringido al centro de Perú y contiene individuos de las localidades tipo de dos formas nominales, G. p. peruana y G. p. junensis, y es hermano de G. aratia. De esta forma, reconocemos dos especies dentro de lo que actualmente se conoce como G. peruana. Restringimos Gastrotheca peruana a las poblaciones del centro de Perú (departamentos de Ancash, Lima, Pasco y Junín) y asignamos Gastrotheca dissimilis a las poblaciones de los departamentos de La Libertad y Cajamarca.

The taxonomic status of Gastrotheca peruana is evaluated using phylogenetic methods and 16S gene sequences. The maximum likelihood and Bayesian trees showed that the genetic variants of G. peruana form two clades that are not sister to each other. One of these clades is present in northern Perú and includes a specimen collected at the type locality of G. peruana dissimilis. The other clade is restricted to central Peru and contains individuals from type localities of two nominal forms, G. p. peruana and G. p. junensis, and is sister to G. aratia. Our results suggest that G. peruana sensu lato currently encompass two species. We restrict Gastrotheca peruana to populations from central Perú (departments of Ancash, Lima, Pasco and Junín) and assign populations from departments of La Libertad and Cajamarca to Gastrotheca dissimilis.

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Se describe el renacuajo de Telmatobius brachydactylus desde el estadio 30 al 41 de Gosner. El renacuajo de T. brachydactylus es similar a las otras larvas de Telmatobius con morfología de tipo poza, pero se diferencia de la especie simpátrica, Telmatobius macrostomus, por tener la cola más larga en relación al cuerpo y una longitud total significativamente menor entre los estadios 33 y 41 de Gosner. Registramos por primera vez renacuajos de T. macrostomus y T. brachydactylus en sintopía.

We describe the tadpole of Telmatobius brachydactylus using specimens from Gosner stages 30 to 41. Telmatobius brachydactylus tadpole is similar to others Telmatobius larvae with pond type morphology, but differs from the sympatric species T. macrostomus by having proportionally a longer tail and significantly a smaller total length between Gosner stages 33 and 41. We recorded for the first time tadpoles of T. macrostomus in syntopy with T. brachydactylus larvae.

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OBJECTIVE: To evaluate the factorial structure of the questionnaire on conceptions about teaching in the domain of health careers in Chile. DESIGN: The cross-sectional analytical study was conducted from July 2016 to December 2016 at different cities in Chile, and comprised healthcare academics in Chile. The questionnaire on conceptions about teaching was used for data collection in addition to a socio-demographic proforma. Exploratory factorial analysis of the questionnaire was performed, and the reliability of the factors located was evaluated through Cronbach's alpha. Descriptive analysis of the data was subsequently performed. RESULTS: Of the 312 subjects, 196(63%) were women and 116(37%) were men. The overall mean age was 45.70}12.04 years (range: 24-73 years). A total of three factors were identified: student as an autonomous learner, professor as a content transmitter, and professor as a trained mediator. These presented reliabilities with Cronbach's alpha between 0.74 and 0.84 and had direct and statistically significant correlations among them (p<0.05). CONCLUSIONS: There was psychometric evidence supporting both validity and reliability of the questionnaire on conceptions about teaching.

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Species delimitation in Phymaturus has been a difficult task due to the highly conserved morphological and ecological features present in this genus. Almost all species of Phymaturus have been described without DNA data or lacking statistical analyses which makes even more difficult to compare species. Although two molecular phylogenetic studies have been recently published, here we provide the first multilocus phylogenetic reconstruction including all Chilean species, with samples from all type localities and some previously unsampled populations. We also estimate pairwise distances among the Chilean species of Phymaturus (P. vociferator and P. mallimaccii clades) and compare our results with the P. payuniae clade, where previous studies have used multiple lines of evidence. Additionally, we performed univariate and multivariate morphological analyses and skeletal comparisons (clavicle) for the species of the P. vociferator clade. As a result of this integrative approach, we describe a new species.

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BACKGROUND: Occupational identity is defined as the perception of occupational interests, abilities, goals, and values, and the structure of the meanings that link these self-perceptions to career role. We developed an occupational identity questionnaire suitable to be used with university teachers. AIM: To evaluate the psychometric properties of the Occupational Identity Questionnaire (OIQ) and relate its results with those of the Teaching Practices questionnaire. MATERIAL AND METHODS: The OIQ and Teaching Practices Questionnaire were answered by 311 university teachers along Chile, who were selected through a non-probabilistic volunteer sampling. An exploratory factor analysis was performed and the internal consistency of each factor was calculated with Cronbach's Alpha. Spearman correlations were used to explore the relationship between the two questionnaires. RESULTS: Two factors were identified in OIQ: Teaching Vocation and Sense of Competence. Cronbach's Alpha was 0.75 for teaching vocation and 0.82 for the sense of competence. There was a moderate direct relationship between teaching vocation, sense of competence and the results of the Teaching Practices Questionnaire. CONCLUSIONS: OIS has an adequate internal consistency, its factorial structure showed two different types of identities and it is directly related to the results of the Teaching Practices Questionnaire.

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Background: Occupational identity is defined as the perception of occupational interests, abilities, goals, and values, and the structure of the meanings that link these self-perceptions to career role. We developed an occupational identity questionnaire suitable to be used with university teachers. Aim: To evaluate the psychometric properties of the Occupational Identity Questionnaire (OIQ) and relate its results with those of the Teaching Practices questionnaire. Material and Methods. The OIQ and Teaching Practices Questionnaire were answered by 311 university teachers along Chile, who were selected through a non-probabilistic volunteer sampling. An exploratory factor analysis was performed and the internal consistency of each factor was calculated with Cronbach's Alpha. Spearman correlations were used to explore the relationship between the two questionnaires. Results: Two factors were identified in OIQ: Teaching Vocation and Sense of Competence. Cronbach's Alpha was 0.75 for teaching vocation and 0.82 for the sense of competence. There was a moderate direct relationship between teaching vocation, sense of competence and the results of the Teaching Practices Questionnaire. Conclusions: OIS has an adequate internal consistency, its factorial structure showed two different types of identities and it is directly related to the results of the Teaching Practices Questionnaire.

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Se presenta el caso de un paciente varón de 56 años quien es evaluado por presentar a nivel del dorso de ambas manos cicatrices hiperpigmentadas e hipopigmentadas, asociadas a quistes de milia. Se le realizó estudios del metabolismo de las porfirinas y biopsia cutánea de las lesiones los cuales resultaron compatibles con porfiria cutánea tarda. En el laboratorio inicial se encontró elevación de los valores de transaminasas, identificándose posteriormente infección crónica por virus de hepatitis C. Con la finalidad de tratar la infección viral y resolver el compromiso dérmico, considerado como manifestación extrahepática del virus hepatitis C, se inició tratamiento con interferón pegilado y ribavirina evolucionando favorablemente con respuesta viral rápida, carga viral no detectable hasta la actualidad (36 semanas de tratamiento), disminución del nivel de transaminasas séricas y mejoría de las lesiones dérmicas.

The present case is a 56 year old male who present hyperpigmented and hypopigmented scars in both hands, associated with the presence of milia cysts. It was studied the metabolism of porphyrins and skin biopsy of the lesions which were compatible with porphyria cutanea tarda. In the initial laboratory, elevated transaminases values were found and subsequently identified chronic infection of hepatitis C virus. In order to treat viral infection and resolve the dermal commitment; considered extrahepatic manifestation of hepatitis C virus, treatment was started with pegylated interferon and ribavirin, with favorably development and rapid viral response, with undetectable viral load until now (24 weeks of treatment), decreased level of serum transaminases and improvement of skin lesions.

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The present case is a 56 year old male who present hyperpigmented and hypopigmented scars in both hands, associated with the presence of milia cysts. It was studied the metabolism of porphyrins and skin biopsy of the lesions which were compatible with porphyria cutanea tarda. In the initial laboratory, elevated transaminases values were found and subsequently identified chronic infection of hepatitis C virus. In order to treat viral infection and resolve the dermal commitment; considered extrahepatic manifestation of hepatitis C virus, treatment was started with pegylated interferon and ribavirin, with favorably development and rapid viral response, with undetectable viral load until now (24 weeks of treatment), decreased level of serum transaminases and improvement of skin lesions.

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UNLABELLED: Introduction and subject: The aim of the study was to determine the factors involved in the delayed medical care of patients with ST-Segment Elevation Myocardial Infarction. METHODS: A prospective observational study was conducted in patients admitted to the coronary care unit at Dr. Juan Graham Casasús hospital with a diagnosis of ST-Segment Elevation Acute Myocardial Infarction. In all patients, clinical data, type and time of reperfusion treatment, and factors associated with delay were identified. RESULTS: Between November 2012 and January 2015 we included 213 patients with ST-Segment Elevation Myocardial Infarction. Age, diabetes, atypical chest angina and patient arrival period (night or weekend), were more frequent in patients presenting after 12 hours of onset of symptoms of myocardial infarction. Of these, hospital admission at night or weekend was the only independent predictor for delay to the emergency room. CONCLUSIONS: This study shows that in a referral hospital in southeast of Mexico, the delay attributable to the patient was the most common factor associated with care in patients with ST-Segment Elevation Myocardial Infarction. Patient arrival period was associated with delay to medical care.

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BACKGROUND: As a metabolic engineering tool, an adaptive laboratory evolution (ALE) experiment was performed to increase the specific growth rate (µ) in an Escherichia coli strain lacking PTS, originally engineered to increase the availability of intracellular phosphoenolpyruvate and redirect to the aromatic biosynthesis pathway. As result, several evolved strains increased their growth fitness on glucose as the only carbon source. Two of these clones isolated at 120 and 200 h during the experiment, increased their µ by 338 and 373 %, respectively, compared to the predecessor PB11 strain. The genome sequence and analysis of the genetic changes of these two strains (PB12 and PB13) allowed for the identification of a novel strategy to enhance carbon utilization to overcome the absence of the major glucose transport system. RESULTS: Genome sequencing data of evolved strains revealed the deletion of chromosomal region of 10,328 pb and two punctual non-synonymous mutations in the dhaM and glpT genes, which occurred prior to their divergence during the early stages of the evolutionary process. Deleted genes related to increased fitness in the evolved strains are rppH, aas, lplT and galR. Furthermore, the loss of mutH, which was also lost during the deletion event, caused a 200-fold increase in the mutation rate. CONCLUSIONS: During the ALE experiment, both PB12 and PB13 strains lost the galR and rppH genes, allowing the utilization of an alternative glucose transport system and allowed enhanced mRNA half-life of many genes involved in the glycolytic pathway resulting in an increment in the µ of these derivatives. Finally, we demonstrated the deletion of the aas-lplT operon, which codes for the main components of the phosphatidylethanolamine turnover metabolism increased the further fitness and glucose uptake in these evolved strains by stimulating the phospholipid degradation pathway. This is an alternative mechanism to its regeneration from 2-acyl-glycerophosphoethanolamine, whose utilization improved carbon metabolism likely by the elimination of a futile cycle under certain metabolic conditions. The origin and widespread occurrence of a mutated population during the ALE indicates a strong stress condition present in strains lacking PTS and the plasticity of this bacterium that allows it to overcome hostile conditions.

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A study was conducted to evaluate the effects of Rosewood oil (RO) on performance, carcass and commercial cut yields and microbiology of the gastrointestinal tract of broiler chickens. Five hundred and forty one-day-old male broiler chicks were arranged in a completely randomized design with six treatments and six repetitions consisting of 15 broiler chicks each. The treatments were: inclusion levels of 0.00mL (0.00EO); 0.15mL (0.15 EO); 0.30mL (0.30 EO); 0.45mL (0.45 EO) and 0.60mL (0.60 EO) of RO/kg diet and control (commercial promoter virginiamycin). At 21 and 40 days old, no significant differences in body weight, feed intake, feed conversion and viability of birds were observed when comparing the controls with the different levels of inclusion of RO. The carcass yields of commercial cuts were not affected by treatments. The increased level of RO reduced the relative weight of the intestines. The broilers consuming growth promoter had the highest concentration of Escherichia coli in the intestinal contents, compared to 0.00 EO and 0.30 EO. It was concluded that, Rosewood oil does affect the performance and yield slaughter, but it does reduce the relative weight of the intestines. RO does not show a consistent antimicrobial activity in vivo against Escherichia coli.

O estudo foi conduzido para investigar os efeitos do óleo essencial de pau rosa sobre o desempenho, rendimento de carcaça e cortes comerciais e microbiologia do trato gastrintestinal de frangos de corte. Foram utilizados 540 pintos machos de um dia de idade, distribuídos num delineamento inteiramente casualizado com seis tratamentos e seis repetições de 15 aves cada. Os tratamentos consistiram em: níveis de inclusão de 0,00mL (0,00 EO); 0,15mL (0,15 EO); 0,30mL (0,30 EO); 0,45mL (0,45 EO) e 0,60mL (0,60 EO) de óleo essencial de pau rosa/kg de ração e controle (promotor comercial virginiamicina). Aos 21 e 40 dias de idade, não foram observadas diferenças significativas para peso corporal, consumo de ração, conversão alimentar e viabilidade das aves quando comparado o tratamento controle e os diferentes níveis de inclusão de RO. Os rendimentos de carcaça e de cortes comerciais não foram influenciados pelos tratamentos. O aumento do nível de inclusão de RO diminuiu o peso relativo dos intestinos. O promotor de crescimento teve maior concentração de Escherichia coli na digesta intestinal quando comparado com 0,00 EO e 0,30 EO. Conclui-se que o óleo essencial de pau rosa não afeta o desempenho e rendimento de abate, reduz o peso relativo dos intestinos. O RO mostra atividade antimicrobiana consistente contra Escherichia coli.

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