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Pseudobombax marginatum, popularly known as "embiratanha," is widely used by traditional communities as anti-inflammatory and analgesic agent. This study aimed to determine the phytochemical profile as well as cytotoxicity, acute oral toxicity, genotoxicity, and mutagenicity attributed to exposure to aqueous (AqEx) and ethanolic (EtEx) extracts of embiratanha bark. Phytochemical screening was conducted using thin-layer chromatography (TLC). Cell viability was analyzed using MTT assay with human mammary gland adenocarcinoma (MDA-MB-231) and macrophage (J774A.1) cell lines, exposed to concentrations of 12.5, 25, 50, or 100 µg/ml of either extract. For acute oral toxicity, comet assay and micronucleus (MN) tests, a single dose of 2,000 mg/kg of either extract was administered orally to Wistar rats. TLC analysis identified classes of metabolites in the extracts, including cinnamic acid derivatives, flavonoids, hydrolyzable tannins, condensed tannins, coumarins, and terpenes/steroids. In the cytotoxicity assay, the varying concentrations of extracts derived from embiratanha induced no significant alterations in the viability of MDA-MB-231 cells. The lowest concentration of EtEx significantly increased macrophage J774A.1 viability. However, the higher concentrations of AqEx markedly lowered macrophage J774A.1 viability. Animals exhibited no toxicity in the parameters analyzed in acute oral toxicity, comet assay, and MN tests. Further, EtEx promoted a significant reduction in DNA damage index and DNA damage frequency utilizing the comet assay, while the group treated with AqEx exhibited no marked differences. Thus, data demonstrated that AqEx or EtEx of embiratanha may be considered safe at a dose of 2,000 mg/kg orgally under our experimental conditions tested.

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This study aimed to characterize the phytochemical profile of bark and leaves aqueous extract Commiphora leptophloeos, and conduct in vivo and in vitro assays to determine the presence of any toxicological consequences due to exposure. The phytochemical analysis was carried out using high-performance liquid chromatography (HPLC). The antioxidant activity was estimated utilizing DPPH free radical scavenging and phosphomolybdenum assays. Cell viability was measured by the MTT method on J774 and human adenocarcinoma cells, which were treated with concentrations of 12,5, 25, 50, 100 or 200 µg/ml of both extracts. Acute oral toxicity, genotoxicity, and mutagenicity assays were determined using a single oral dose of 2000 g/kg in male Swiss albino mice (Mus musculus). Biochemical analysis of the blood and histological analyses of the kidneys, liver, spleen, pylorus, duodenum and jejunum were undertaken. Genotoxicity and mutagenicity were determined utilizing blood samples. Gallic acid, catechin, and epicatechin were identified in the bark and chlorogenic acid in leaves. Data demonstrated a high content of phenolic compounds and flavonoids associated with significant antioxidant potential. No significant signs in damage or symptoms of toxicity were detected. No marked reduction in cell viability was found at lower concentrations tested. On histomorphometry, only the gastrointestinal organs exhibited significant difference. Renal hepatic and blood parameters were within the normal range. No apparent signs of toxicity, genotoxicity, mutagenicity or cytotoxicity were found in vivo and in vitro experiments.

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INTRODUCTION: Despite numerous scientific advances, cancer continues to be one of the main causes of death in the world. This situation has driven the search for promising molecules. Lichen substances have been widely described for their pharmacological potential. OBJECTIVE: The present study evaluated the antitumour potential of a depsidone isolated from Parmotrema concurrens- salazinic acid (SAL) - through in vitro, in vivo and in silico studies. METHODS: The molecule was isolated from the acetonic extract of the lichen and recrystallized in acetone. The macrophage J774, sarcoma-180 and MDA-MB-231 cell lines were used for the MTT cytotoxicity assay. The antitumor assay used a murine model (Swiss albino mice) with sarcoma-180. The animals were treated for seven consecutive days with doses of SAL (25 and 50 mg/kg) and 5-fluorouracil (20 mg/kg). RESULTS: Its purity was determined using high-performance liquid chromatography (94%), and its structure was confirmed by H1 and C13 nuclear magnetic resonance. SAL was not considered toxic to cancer cell lines, showing cell viability rates of 79.49 ± 4.15% and 86.88 ± 1.02% for sarcoma-180 and MDA-MB-231, respectively. The tumour inhibition rate was greater than 80% in the animals treated with SAL and 65% for those that received 5-fluorouracil. Simulations of molecular dynamics to estimate the flexibility of the interactions between human thymidylate synthase and derivatives of SAL and 5-fluorouracil revealed that SAL exhibited greater enzymatic interaction capacity, with highly favourable energy, compared to 5-fluorouracil. CONCLUSION: The present results demonstrate the potential of salazinic acid as a tumour inhibition agent.

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AIMS: This study aimed to evaluate the gastroduodenal protective action of crude fraction extracted from P. caribaeorum mucus in Wistar rats. MAIN METHOD: Initially, phytochemical screening was performed to measure secondary metabolites present in the extract. Subsequently, studies of gastroprotective action in Wistar rats were developed. The animals were randomly divided into six experimental groups: SF0.9% group, misoprostol group, and test groups (200, 100, 10, and 1 mg/kg) that received different doses of the crude fraction of zoanthid mucus (CFZM) diluted in SF0.9%. After 14 days of treatment, acute gastric ulcers were induced by gavage by administering aspirin (200 mg/kg). The stomach and duodenum were removed for histopathological and gene analysis of the mucosa. KEY FINDINGS: The present study found that all investigated metabolites showed negative results. The crude fraction showed a gastric and duodenal protective effect evidenced by an increase in the amount and production of mucins (MUC1 and MUC5AC) and mucus production area in the stomach. Histopathological analysis evidenced a decrease in epithelial damage in the duodenum, with a more significant extension of intestinal villi and a greater amount of goblet cells. SIGNIFICANCE: The crude fraction, extracted from P. caribaeorum, showed gastric and duodenal protective action and is not inert in murine gastroduodenal tissues.

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BACKGROUND: Cerebral palsy (CP) associates cerebral function damages with strong locomotor defects and premature sarcopenia. We previously showed that fibroblast growth factor 19 (FGF19) exerts hypertrophic effects on skeletal muscle and improves muscle mass and strength in mouse models with muscle atrophy. Facing the lack of therapeutics to treat locomotor dysfunctions in CP, we investigated whether FGF19 treatment could have beneficial effects in an experimental rat model of CP. METHODS: Cerebral palsy was induced in male Wistar rat pups by perinatal anoxia immediately after birth and by sensorimotor restriction of hind paws maintained until Day 28. Daily subcutaneous injections with recombinant human FGF19 (0.1 mg/kg bw) were performed from Days 22 to 28. Locomotor activity and muscle strength were assessed before and after FGF19 treatment. At Day 29, motor coordination on rotarod and various musculoskeletal parameters (weight of tibia bone and of soleus and extensor digitorum longus (EDL) muscles; area of skeletal muscle fibres) were evaluated. In addition, expression of specific genes linked to human CP was measured in rat skeletal muscles. RESULTS: Compared to controls, CP rats had reduced locomotion activity (-37.8% of distance travelled, P < 0.05), motor coordination (-88.9% latency of falls on rotarod, P < 0.05) and muscle strength (-25.1%, P < 0.05). These defects were associated with reduction in soleus (-51.5%, P < 0.05) and EDL (-42.5%, P < 0.05) weight, smaller area of muscle fibres, and with lower tibia weight (-38%, P < 0.05). In muscles from rats submitted to CP, changes in the expression levels of several genes related to muscle development and neuromuscular junctions were similar to those found in wrist muscle of children with CP (increased mRNA levels of Igfbp5, Kcnn3, Gdf8, and MyH4 and decreased expression of Myog, Ucp2 and Lpl). Compared with vehicle-treated CP rats, FGF19 administration improved locomotor activity (+53.2%, P < 0.05) and muscle strength (+25.7%, P < 0.05), and increased tibia weight (+13.8%, P < 0.05) and soleus and EDL muscle weight (+28.6% and +27.3%, respectively, P < 0.05). In addition, it reduced a number of very small fibres in both muscles (P < 0.05). Finally, gene expression analyses revealed that FGF19 might counteract the immature state of skeletal muscles induced by CP. CONCLUSIONS: These results demonstrate that pharmacological intervention with recombinant FGF19 could restore musculoskeletal and locomotor dysfunction in an experimental CP model, suggesting that FGF19 may represent a potential therapeutic strategy to combat the locomotor disorders associated with CP.

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O consumo de bebidas alcoólicas na gravidez consiste em um importante problema de saúde pública, visto que, pode causar prejuízos na organogênese de diversos órgãos, incluindo o estômago, entretanto, poucos estudos avaliam o efeito da exposição pré-natal ao álcool nesse órgão. O objetivo deste estudo foi analisar histologicamente o estômago da prole de ratas submetidas ao consumo crônico de álcool durante a prenhez. Utilizou-se 10 ratas prenhes divididas nos grupos: Controle - ratas que receberam água destilada durante todo período gestacional e Álcool ­ ratas que receberam álcool etílico absoluto (3g/kg/dia) durante todo período gestacional. Logo após o nascimento, 12 neonatos (6 machos e 6 fêmeas) de cada grupo foram anestesiados e os estômagos coletados. Posteriormente, os órgãos foram fixados e processados seguindo a técnica histológica de rotina. Foram feitas análises histomorfométricas das camadas mucosa, muscular e da parede total do estômago. Observou-se que as proles macho e fêmea expostas ao etanol apresentaram diminuição da área de epitélio, contudo, os machos também mostraram redução significativa do número de células epiteliais. Demonstrou-se ainda redução na espessura das camadas mucosa, muscular e da parede total do estômago da prole fêmea do grupo Álcool. No entanto, a camada muscular apresentou aumento significativo em sua espessura no grupo de neonatos machos expostos ao etanol. Assim, concluímos que a exposição pré-natal ao álcool provoca efeitos nocivos sobre o estômago dos neonatos, contudo, estudos futuros são necessários para melhor elucidar os mecanismos envolvidos na patogênese e possíveis consequências para os animais na fase adulta.

Consumption of alcoholic beverages during pregnancy is a significant public health issue since it can damage the organogenesis of several organs, including the stomach; however, few studies evaluate the effect of prenatal exposure to alcohol in this organ. The objective of this study was to analyze the histology of the stomach of offspring of rats submitted to chronic alcohol consumption during pregnancy. Ten pregnant rats were divided into two groups: Control - rats receiving distilled water throughout the gestation period, and Alcohol - rats receiving absolute ethyl alcohol (3g/kg/day) throughout the gestation period. After birth, 12 newborn rats (6 males and 6 females) from each group were anesthetized and their stomachs were collected. Subsequently, the organs were fixed and processed following the routine histological technique. The mucosa, muscle and total stomach were submitted to histomorphometric analyses. It was observed that the male and female offspring exposed to ethanol had a decrease in the epithelium area. However, males also showed a significant reduction in the number of epithelial cells. There was also a reduction in the layer's thickness mucosa, muscle and total stomach wall of the female offspring from the alcohol group. Additionally, the muscular layer presented a significant increase in its thickness in the group of male neonates exposed to ethanol. It can be concluded that prenatal exposure to alcohol causes harmful effects on neonates' stomachs; however, future studies are necessary to better elucidate the mechanisms involved in the pathogenesis and possible consequences for the animals in adulthood.

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There is a strong correlation between inadequate gestational and postpartum nutrition and the occurrence of cardiovascular diseases. The present study investigated the effects of a maternal low-protein diet and neonatal overfeeding on the oxidative balance and morphology of the renal cortex of male Wistar rats. Two independent protocols were used. First, pregnant Wistar rats received diets containing either 17% (normal protein) or 8% (low protein) casein throughout pregnancy and lactation. Second, the litter size was reduced by one-third on the third postnatal day to induce overnourishment in offspring. At 30 days, the oxidative balance and morphology of the renal cortex were analyzed. There was a small but significant increase in renal corpuscle area in the low protein (LP, 5%) and overnutrition (ON, 8%) groups. Glomerular tuft area also increased in LP (6%) and ON (9%), as did glomerular cellularity (LP, +11%; ON, +12%). In the oxidative stress analyses, both nutritional insults significantly elevated lipid peroxidation (LP, +18%; ON, +135%) and protein oxidation (LP, +40%; ON, +65%) while significantly reducing nonenzymatic antioxidant defenses, measured as reduced glutathione (LP, -32%; ON, -45%) and total thiol content (LP, -28%; ON, -24%). We also observed a decrease in superoxide dismutase (LP, -78%; ON, -51%), catalase (LP, -18%; ON, -61%), and glutathione S-transferase (only in ON, -44%) activities. Our results demonstrate that nutritional insults, even those of a very different nature, during perinatal development can result in similar changes in oxidative parameters and glomerular morphology in the renal cortex.

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Laboratory maintenance of the Schistosoma mansoni cycle is necessary for developing studies regarding the diagnosis, treatment and control of schistosomiasis. Within this perspective, it is paramount that mice infected by the parasite should present a minimum survival of six months. However, between October 2016 and May 2017, early deaths were observed among infected animals kept in the vivarium of the Schistosomiasis Reference Service of IAMFIOCRUZ. Therefore, the purpose of the present study was to present the results obtained after investigating the main cause of death among these animals. To achieve this, animals that died or that needed to be euthanized due to clinical distress caused by parasite infection were necropsied to investigate the cause of death and clinical condition. Fragments from the intestines, mesenteric vessels and livers were removed and were subjected to histopathological studies. In addition, mouse feces were collected and analyzed using the hydrogen peroxide reaction to detect occult blood. Over an eight-month period, 70 deaths were noted. Forty two animals presented mesenteric ischemia, a vascular insufficiency syndrome that causes a reduction in the nutrient supply to the intestinal viscera. There is, therefore, a need to reduce the infective parasite load in mice to increase their survival, reduce distress caused by the infection and ensure maintenance of the S. mansoni cycle, thus enabling continuity of scientific studies on this parasitosis.

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Abstract The occurrence of Manson's schistosomiasis in organs of the female reproductive tract is an uncommon event, given that the etiological agent for this disease is a blood parasite that inhabits the mesenteric veins. In this case report, a 45-year-old female patient reported that her first symptoms had been strong pain in the left iliac region around two years earlier. An endovaginal pelvic ultrasonography showed that the left ovary was enlarged, and the report suggested that this finding might be correlated with clinical data and tumor markers. After being examined at several healthcare services, the patient was referred to an oncology service due to suspected neoplasia, where she underwent a left ovariectomy. The result from the histopathological examination showed the presence of granulomatous inflammatory processes surrounding both viable and calcified eggs of Schistosoma mansoni. There was no evidence of any neoplastic tissue. The patient was medicated and followed-up as an outpatient.

Resumo A ocorrência da esquistossomose mansônica em órgãos do aparelho reprodutor feminino é um evento pouco comum, tendo em vista que o agente etiológico desta doença é um parasito sanguíneo que habita as veias mesentéricas. Neste relato decaso, uma paciente de 45 anos referiu como primeira sintomatologia fortes dores na região ilíaca esquerda há cerca de 2 anos. Uma ultrassonografia pélvica endovaginal identificou aumento do ovário esquerdo, e o laudo sugeriu correlacionar tal achado com dados clínicos e marcadores tumorais. Após passar por vários serviços de saúde, a paciente foi encaminhada para um serviço de oncologia por suspeita de neoplasia, sendo submetida a uma ovariectomia à esquerda. O resultado do exame histopatológico evidenciou a presença de processos inflamatórios granulomatosos em torno de ovos viáveis e calcificados de Schistosoma mansoni. Não houve qualquer evidência de tecido neoplásico. A paciente foi medicada, e seguiu em acompanhamento ambulatorial.

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The occurrence of Manson's schistosomiasis in organs of the female reproductive tract is an uncommon event, given that the etiological agent for this disease is a blood parasite that inhabits the mesenteric veins. In this case report, a 45-year-old female patient reported that her first symptoms had been strong pain in the left iliac region around two years earlier. An endovaginal pelvic ultrasonography showed that the left ovary was enlarged, and the report suggested that this finding might be correlated with clinical data and tumor markers. After being examined at several healthcare services, the patient was referred to an oncology service due to suspected neoplasia, where she underwent a left ovariectomy. The result from the histopathological examination showed the presence of granulomatous inflammatory processes surrounding both viable and calcified eggs of Schistosoma mansoni. There was no evidence of any neoplastic tissue. The patient was medicated and followed-up as an outpatient.

A ocorrência da esquistossomose mansônica em órgãos do aparelho reprodutor feminino é um evento pouco comum, tendo em vista que o agente etiológico desta doença é um parasito sanguíneo que habita as veias mesentéricas. Neste relato de caso, uma paciente de 45 anos referiu como primeira sintomatologia fortes dores na região ilíaca esquerda há cerca de 2 anos. Uma ultrassonografia pélvica endovaginal identificou aumento do ovário esquerdo, e o laudo sugeriu correlacionar tal achado com dados clínicos e marcadores tumorais. Após passar por vários serviços de saúde, a paciente foi encaminhada para um serviço de oncologia por suspeita de neoplasia, sendo submetida a uma ovariectomia à esquerda. O resultado do exame histopatológico evidenciou a presença de processos inflamatórios granulomatosos em torno de ovos viáveis e calcificados de Schistosoma mansoni. Não houve qualquer evidência de tecido neoplásico. A paciente foi medicada, e seguiu em acompanhamento ambulatorial.

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Schistosomiasis has affected the rural population of Pernambuco, Brazil, for centuries. The hepatosplenic and neurological manifestations of this parasitosis have often been described. However, prostatic schistosomiasis caused by Schistosoma mansoni had never been registered in Pernambuco, thus the importance of this particular case being reported. Case presentation: This report records the first case of prostatic schistosomiasis in Pernambuco. A 51-year-old patient underwent partial prostatectomy due to possible cancer and was diagnosed with this ectopic form of schistosomiasis. After the surgical procedure, stool samples were collected to run Kato-Katz parasitological tests, which were positive for S. mansoni, thus confirming that the patient was still infected. Laboratory blood tests and clinical examination showed alterations in liver function and confirmed the presence of hepatointestinal damage. Patient monitoring evidenced that the prostate-specific antigen levels remained high and, one year after the first surgical intervention, a new prostatic puncture showed that schistosomiasis and fibromatosis lesions remained present. It is noteworthy that after triple praziquantel treatment (April 2014, July 2014, February 2015) parasitological stool examinations were all negative for S. mansoni. In conclusion this accidental diagnosis of prostatic schistosomiasis raises doubts regarding the ability of healthcare services to identify and treat ectopic schistosomiasis. The persistently high levels of PSA even after surgical and pharmacological treatment, indicate irreversible damage to tissue caused by S. mansoni. Therefore, healthcare services need to be prepared to investigate and diagnose these cases, with a view to preventing chronic sequelae through early treatment

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The Southern Crested Carcara is a bird with a recognized ecological role in the population control of small birds and mammals, feeding on live prey as much as on decaying animals. The objective of this study was to analyze the histology of Carcara`s intestinal tract of five Carcaras from the Wild Animal Screening Center of Pernambuco were euthanized to remove their intestines and cloacae. The tissues were stained with Hematoxylin/Eosin, Alcian Blue pH 1.0, and Periodic Acid Schiff. The villi of the small and large intestines are lined with a simple columnar epithelium with microvilli membranes and goblet cells. The lamina contains lymphocytes and form lymphatic follicles within some of the villi. The mucosal muscle is arranged in a longitudinal direction. The submucosal layer was only observed in the cloaca and the muscle layer has transverse and longitudinal fibers. The serous membrane lines the whole intestinal tract and is composed of mesothelium and submesothelial connective tissue. Longitudinal folds were found in the large intestine. The conclusion is that the Carcara`s intestine exhibits an overall structure compatible with the histological pattern observed in other species of birds; however, Paneth cells and the submucosal layer were not observed. Lymph follicles were found inside the villi and a high number of goblet cells at the end of the ileum and large intestine.(AU)

O carcará é uma ave com reconhecido papel ecológico, atuando no controle de populações de pequenas aves e mamíferos, alimentando-se tanto de presas vivas, quanto de animais em decomposição. Objetivou-se analisar a histologia do trato intestinal do carcará. Foram eutanasiados cinco carcarás provenientes do Centro de Triagem de Animais Silvestres de Pernambuco, para retirada do intestino e cloaca. Os tecidos foram corados por Hematoxilina/Eosina, Alcian Blue pH 1,0 e Ácido Periódico de Shiff. As vilosidades do intestino delgado e grosso são revestidas por epitélio simples colunar, com microvilosidades e células caliciformes. A lâmina própria contém linfócitos, formando folículos linfáticos no interior de algumas vilosidades. Já a muscular da mucosa está disposta no sentido longitudinal. A camada submucosa só foi observada na cloaca e a camada muscular possui fibras transversais e longitudinais. A serosa reveste externamente todo trato intestinal, sendo composta por mesotélio e tecido conjuntivo submesotelial. Pregas longitudinais foram encontradas no intestino grosso. Conclui-se que o intestino do Carcará apresenta de forma geral estrutura compatível com o padrão histológico observado em outras espécies de aves; entretanto, as células de Paneth e camada submucosa não foram observadas. Folículos linfáticos foram encontrados no interior das vilosidades e um elevado número de células caliciformes na porção final do íleo e intestino grosso.(AU)

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Abstract The Southern Crested Carcara is a bird with a recognized ecological role in the population control of small birds and mammals, feeding on live prey as much as on decaying animals. The objective of this study was to analyze the histology of Carcara`s intestinal tract of five Carcaras from the Wild Animal Screening Center of Pernambuco were euthanized to remove their intestines and cloacae. The tissues were stained with Hematoxylin/Eosin, Alcian Blue pH 1.0, and Periodic Acid Schiff. The villi of the small and large intestines are lined with a simple columnar epithelium with microvilli membranes and goblet cells. The lamina contains lymphocytes and form lymphatic follicles within some of the villi. The mucosal muscle is arranged in a longitudinal direction. The submucosal layer was only observed in the cloaca and the muscle layer has transverse and longitudinal fibers. The serous membrane lines the whole intestinal tract and is composed of mesothelium and submesothelial connective tissue. Longitudinal folds were found in the large intestine. The conclusion is that the Carcara`s intestine exhibits an overall structure compatible with the histological pattern observed in other species of birds; however, Paneth cells and the submucosal layer were not observed. Lymph follicles were found inside the villi and a high number of goblet cells at the end of the ileum and large intestine.

Resumo O carcará é uma ave com reconhecido papel ecológico, atuando no controle de populações de pequenas aves e mamíferos, alimentando-se tanto de presas vivas, quanto de animais em decomposição. Objetivou-se analisar a histologia do trato intestinal do carcará. Foram eutanasiados cinco carcarás provenientes do Centro de Triagem de Animais Silvestres de Pernambuco, para retirada do intestino e cloaca. Os tecidos foram corados por Hematoxilina/Eosina, Alcian Blue pH 1,0 e Ácido Periódico de Shiff. As vilosidades do intestino delgado e grosso são revestidas por epitélio simples colunar, com microvilosidades e células caliciformes. A lâmina própria contém linfócitos, formando folículos linfáticos no interior de algumas vilosidades. Já a muscular da mucosa está disposta no sentido longitudinal. A camada submucosa só foi observada na cloaca e a camada muscular possui fibras transversais e longitudinais. A serosa reveste externamente todo trato intestinal, sendo composta por mesotélio e tecido conjuntivo submesotelial. Pregas longitudinais foram encontradas no intestino grosso. Conclui-se que o intestino do Carcará apresenta de forma geral estrutura compatível com o padrão histológico observado em outras espécies de aves; entretanto, as células de Paneth e camada submucosa não foram observadas. Folículos linfáticos foram encontrados no interior das vilosidades e um elevado número de células caliciformes na porção final do íleo e intestino grosso.

RESUMO

The Southern Crested Carcara is a bird with a recognized ecological role in the population control of small birds and mammals, feeding on live prey as much as on decaying animals. The objective of this study was to analyze the histology of Carcara`s intestinal tract of five Carcaras from the Wild Animal Screening Center of Pernambuco were euthanized to remove their intestines and cloacae. The tissues were stained with Hematoxylin/Eosin, Alcian Blue pH 1.0, and Periodic Acid Schiff. The villi of the small and large intestines are lined with a simple columnar epithelium with microvilli membranes and goblet cells. The lamina contains lymphocytes and form lymphatic follicles within some of the villi. The mucosal muscle is arranged in a longitudinal direction. The submucosal layer was only observed in the cloaca and the muscle layer has transverse and longitudinal fibers. The serous membrane lines the whole intestinal tract and is composed of mesothelium and submesothelial connective tissue. Longitudinal folds were found in the large intestine. The conclusion is that the Carcara`s intestine exhibits an overall structure compatible with the histological pattern observed in other species of birds; however, Paneth cells and the submucosal layer were not observed. Lymph follicles were found inside the villi and a high number of goblet cells at the end of the ileum and large intestine.

O carcará é uma ave com reconhecido papel ecológico, atuando no controle de populações de pequenas aves e mamíferos, alimentando-se tanto de presas vivas, quanto de animais em decomposição. Objetivou-se analisar a histologia do trato intestinal do carcará. Foram eutanasiados cinco carcarás provenientes do Centro de Triagem de Animais Silvestres de Pernambuco, para retirada do intestino e cloaca. Os tecidos foram corados por Hematoxilina/Eosina, Alcian Blue pH 1,0 e Ácido Periódico de Shiff. As vilosidades do intestino delgado e grosso são revestidas por epitélio simples colunar, com microvilosidades e células caliciformes. A lâmina própria contém linfócitos, formando folículos linfáticos no interior de algumas vilosidades. Já a muscular da mucosa está disposta no sentido longitudinal. A camada submucosa só foi observada na cloaca e a camada muscular possui fibras transversais e longitudinais. A serosa reveste externamente todo trato intestinal, sendo composta por mesotélio e tecido conjuntivo submesotelial. Pregas longitudinais foram encontradas no intestino grosso. Conclui-se que o intestino do Carcará apresenta de forma geral estrutura compatível com o padrão histológico observado em outras espécies de aves; entretanto, as células de Paneth e camada submucosa não foram observadas. Folículos linfáticos foram encontrados no interior das vilosidades e um elevado número de células caliciformes na porção final do íleo e intestino grosso.

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Este estudo analisou a condição reprodutiva, por meio da histomorfometria, de P. discolor coletados em fragmentos de Mata Atlântica do litoral sul de Pernambuco, durante as estações seca e chuvosa. Os animais são de coleção e foram classificados de acordo com a posição testicular (descendentes e não descendentes). Para as análises histomorfométricas, foram selecionados aleatoriamente 18 espécimes durante as estações seca e chuvosa, dos quais (n = 11) com testículos descendentes e (n = 7) com testículos não descendentes. Os resultados demonstraram que as maiores médias da área de ocupação dos túbulos seminíferos foram na estação chuvosa, independente dos espécimes apresentarem os testículos descendentes ou não. Isso pode estar relacionado a um maior investimento em produção espermática, já que na estação chuvosa, existe uma maior disponibilidade de alimentos devido às precipitações pluviométricas.

This study analyzed the reproductive condition, by histomorphometry, of P. discolor collected in forest fragments of the South Coast of Pernambuco during the dry and rainy seasons. The animals are Collection and were classified according to the position testicular (descendants and not descendants). For histomorphometric analysis, 18 were randomly selected specimens during the rainy and dry seasons, being (n = 11) with descendant testicles and (n = 7) with testicles no descendant. The results showed that the highest area average occupancy of the seminiferous tubules were in the rainy season, regardless of the present specimens the testes descendant or not. This may be related to a greater investment in sperm production, since the rainy season, there is a greater availability of food due to rainfall.

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RESUMO

Este estudo analisou a condição reprodutiva, por meio da histomorfometria, de P. discolor coletados em fragmentos de Mata Atlântica do litoral sul de Pernambuco, durante as estações seca e chuvosa. Os animais são de coleção e foram classificados de acordo com a posição testicular (descendentes e não descendentes). Para as análises histomorfométricas, foram selecionados aleatoriamente 18 espécimes durante as estações seca e chuvosa, dos quais (n = 11) com testículos descendentes e (n = 7) com testículos não descendentes. Os resultados demonstraram que as maiores médias da área de ocupação dos túbulos seminíferos foram na estação chuvosa, independente dos espécimes apresentarem os testículos descendentes ou não. Isso pode estar relacionado a um maior investimento em produção espermática, já que na estação chuvosa, existe uma maior disponibilidade de alimentos devido às precipitações pluviométricas.(AU)

This study analyzed the reproductive condition, by histomorphometry, of P. discolor collected in forest fragments of the South Coast of Pernambuco during the dry and rainy seasons. The animals are Collection and were classified according to the position testicular (descendants and not descendants). For histomorphometric analysis, 18 were randomly selected specimens during the rainy and dry seasons, being (n = 11) with descendant testicles and (n = 7) with testicles no descendant. The results showed that the highest area average occupancy of the seminiferous tubules were in the rainy season, regardless of the present specimens the testes descendant or not. This may be related to a greater investment in sperm production, since the rainy season, there is a greater availability of food due to rainfall.(AU)

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RESUMO

A dermatomiosite é uma doença rara, que se caracteriza pelo comprometimento da pele e músculos estriados, sendo classificada como doença auto-imune. A etiologia e a etiopatogenia da dermatomiosite permanecem obscuras. Como é uma doença sistêmica, suas manifestações clínicas säo de interesse de diversas áreas das ciências médicas, exigindo a interaçäo entre profissionais de especialidades diferentes, como clínicos gerais, dermatologistas, oftalmologistas, cardiologistas, estomatologistas, fisioterapeutas, fonoaudiólogos e neneticistas. Este trabalho descreve os aspectos clínicos, histopatológicos e terapêuticos desta moléstia

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