RESUMO

Fermented soybean grain (FSBG) is considered improper to use as a protein source in animal nutrition, since it is assumed that defects cause changes on its chemical composition and favor mycotoxins production, but chemical composition data does not support this theory and in vivo studies are missing. Thus, this study aimed to evaluate the effects of FSBG in feedlot lamb diets. For that, two types of FSBG (partially fermented and completely fermented, PFSBG and CFSBG) and one standard soybean grain (SSBG) were obtained and evaluated alone or as a component of experimental diets by in vitro and in vivo studies, where FSBG totally replaced SSBG in feedlot lamb diets, which was included in the experimental diets in 17.4% on dry matter basis as protein source. Before the studies, both soybeans were sent to a specialized laboratory where no mycotoxins were detected. As a result, lower DM and carbohydrate contents but higher crude protein, fiber, and indigestible NDF contents were measured in CFSBG than in SSBG. Furthermore, both types of FSBG showed lower digestibility in vitro dry matter (IVDMD) than SSBG when evaluated separately; however, when evaluated in experimental diets, the substitution of SSBG for FSBG did not affect IVDMD. It was also observed that FSBG also had less rumen-degradable protein than SSBG (mean 47.9 vs 86.4%). In the in vivo study, FSBG did not affect nutrient intake, apparent digestibility, or animal performance (i.e., average daily gain and carcass gain). Thus, mycotoxins-free FSBG may be an alternative to totally replace SSBG in feedlot lamb diets.

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The objective of this study was to evaluate the effects of the daily supplementation with a low dosage of a blend of condensed and hydrolysable tannins on the in vitro and in situ digestibility of protein and fibrous feedstuffs. In situ ruminal incubation assays were conducted on seven protein and five roughage feedstuffs with and without tannin supplementation (1 g kg-1 DM intake). From these same cattle, rumen Mundim, A. T. et al.1146Semina: Ciênc. Agrár. Londrina, v. 44, n. 3, p. 1145-1166, maio/jun. 2023fluid was collected for in vitro incubations of the same feedstuffs. In vitro assays we evaluated the gas production (GP) up to 24 h and, sequentially, digestibility of dry matter (IVDMD), crude protein (IVCPD), fiber (IVNDFD), and ammonia nitrogen (NH3-N). For in situ assays, the disappearance curves were modeled and then the potentially degradable (B), digestion rate (kd), and undegradable (U) fractions were estimated. Of all the variables studied, only IVCPD of soybean meal showed effect (P<0.05) with the addition of dose of tannins. The supplementation of tannin affected only B of the DM and CP of the cottonseed meal. The kd of DM and CP increased with the supplementation of the tannin blend only for Marandu (dry season), soybean and cottonseed meal. Additionally, the kd of the DM of corn silage was reduced. The U of CP was affected by the increase of tannin supplementation for soybean and cottonseed meal. Daily supplementation with a low dose of tannin blend for grazing cattle affects for grazing cattle affects the ruminal digestibility of some of the evaluated feedstuffs, which are soybean and cottonseed meal.(AU)

O objetivo com este estudo foi avaliar os efeitos da inclusão de uma mistura de taninos condensados e hidrolisáveis em suplementos sobre a digestibilidade in vitro e in situ de alguns alimentos proteicos e volumosos. Sete alimentos proteicos e cinco volumosos foram avaliados in situ no rúmen de bovinos de corte em pastejo recebendo suplementos com e sem taninos (dose de taninos = 1g kg-1 MS). Destes mesmos animais, foi coletado líquido ruminal para incubação in vitro dos alimentos. Nos ensaios in vitro avaliou-se a produção de gás (PG) até 24 h e, sequencialmente, a digestibilidade da matéria seca (DMS), proteína bruta (DPB), fibra (DFDN) e nitrogênio amoniacal (N-NH3). Para os ensaios in situ, as curvas de desaparecimento foram modeladas e, em seguida, as frações potencialmente degradáveis (B), taxa de digestão (kd) e indigestível (U) foram estimadas. De todas as variáveis estudadas, apenas a DPB do farelo de soja apresentou efeito (P<0,05) com a adição de taninos. A suplementação com taninos afetou apenas a fração B da MS e PB do farelo de algodão. O kd da MS e PB aumentaram com a inclusão de taninos para o capim Marandu (estação seca), farelo de soja e farelo de algodão. Adicionalmente, o kd da MS da silagem de milho foi reduzido. A fração indisgestível (U) da PB do farelo de soja e farelo de algodão foram afetadas pela suplementação com taninos. A inclusão de baixas doses de um blend de taninos em suplementos para bovinos em pasto afeta a digestibilidade ruminal de alguns dos alimentos avaliados, notadamente em farelo de soja e farelo de algodão.(AU)

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El objetivo de este trabajo fue evaluar modelos matemáticos comparando los parámetros cinéticos en la producción de gas in vitro en diferentes genotipos de pasto elefante. El diseño fue completamente al azar con tres repeticiones con cinco tratamientos que fueron los genotipos de pasto elefante (BRS Canará, Napier, Vruckwona, Camerún y CNPGL 93-41-1) con una madurez de rebrote a los 45 días. El inóculo ruminal se obtuvo de dos novillos Nelores machos mantenidos en potreros formados por Urochloa brizantha cv. Marandu, en el período seco del año. Las incubaciones fueron consecutivas midiendo las lecturas de presión de producción de gas realizadas en 2; 4; 6; 8; 10; 12; 19; 24; 36; 48; 60; 72; 84 y 96 horas. La determinación de la cinética de producción de gas se probó en dos modelos no lineales, que son: Monomolecular y Logístico. Donde se estimaron el volumen final (V_f / mL), la tasa de producción de gas promedio (k / h-1), el tiempo de latencia de producción (L / h) y el tiempo de digestión promedio (TMD / h-1). Los modelos probados, el Logístico con latencia, presentaron el mejor ajuste para todos los perfiles de cultivares. El cultivar BRS Canará y CNPGL 93-41-1 y Wrockwona, presentan estimaciones de los parámetros cinéticos de producción de gas similares y mejores a los de los cultivares Camerún y Napier.(AU)

The aim of this study was to evaluate mathematical models comparing the kinetic parameters in the production of in vitro gas in different genotypes of elephant grass The design was entirely randomized with three repetitions with five treatments that were the elephant grass genotypes (BRS Canará, Napier, Vruckwona, Cameroon and CNPGL 93-41-1) with age of re-growth at 45 days. The ruminal inoculum was obtained from two whole male Nelores oxen kept in pickets formed by Urochloa brizantha cv. Marandu in the dry period of the year. The incubations were consecutive, measuring the gas production pressure readings in 2; 4; 6; 8; 10; 12; 19; 24; 36; 48; 60; 72; 84 and 96 hours. The determination of gas production kinetics was tested in two non-linear models: Monomolecular and Logistic. Where the final volume (Vf/mL), average gas production rate (k/h-1), production lage time (Lag/h) and average digestion time (MDT/h) were estimated. The models tested, the Logistic with latency showed better fit for all crop profiles. The BRS Canará and CNPGL 93-41-1 and Wrockwona cultivars present estimates of kinetic parameters of gas production similar and better than those of Cameroon and Napier cultivars.(AU)

Objetivou-se neste trabalho avaliar modelos matemáticos comparando os parâmetros cinéticos na produção de gás in vitro em diferentes genótipos de capim-elefante. O delineamento foi inteiramente casualizados com três repetições com cincos tratamentos que foram os genótipos de capim-elefante (BRS Canará, Napier, Vruckwona, Cameroon e CNPGL 93-41-1) com idade de maturidade de rebrota aos 45 dias. O inóculo ruminal foi obtido através de dois bois Nelores machos inteiros mantidos em piquetes formados por Urochloa brizantha cv. Marandu, no período seco do ano. As incubações foram consecutivas mensurando as leituras de pressão de produção de gás realizadas em 2; 4; 6; 8; 10; 12; 19; 24; 36; 48; 60; 72; 84 e 96 horas. A determinação da cinética de produção de gases foi testada dois modelos não lineares que são: Monomolecular e o Logístico. Onde foram estimados o volume final ( /mL), taxa média de produção de gás ( /h-1), o tempo de latência de produção ( /h) e o tempo médio de digestão (TMD/h-1). Os modelos testados, o Logístico com latência apresentou melhor ajuste para todos os perfis das cultivares. A cultivar BRS Canará e CNPGL 93-41-1 e Wrockwona, apresentam estimativas dos parâmetros cinéticos de produção de gás semelhantes e melhores aos comparados das cultivares Cameroon e Napier.(AU)

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El objetivo de este trabajo fue evaluar modelos matemáticos comparando los parámetros cinéticos en la producción de gas in vitro en diferentes genotipos de pasto elefante. El diseño fue completamente al azar con tres repeticiones con cinco tratamientos que fueron los genotipos de pasto elefante (BRS Canará, Napier, Vruckwona, Camerún y CNPGL 93-41-1) con una madurez de rebrote a los 45 días. El inóculo ruminal se obtuvo de dos novillos Nelores machos mantenidos en potreros formados por Urochloa brizantha cv. Marandu, en el período seco del año. Las incubaciones fueron consecutivas midiendo las lecturas de presión de producción de gas realizadas en 2; 4; 6; 8; 10; 12; 19; 24; 36; 48; 60; 72; 84 y 96 horas. La determinación de la cinética de producción de gas se probó en dos modelos no lineales, que son: Monomolecular y Logístico. Donde se estimaron el volumen final (V_f / mL), la tasa de producción de gas promedio (k / h-1), el tiempo de latencia de producción (L / h) y el tiempo de digestión promedio (TMD / h-1). Los modelos probados, el Logístico con latencia, presentaron el mejor ajuste para todos los perfiles de cultivares. El cultivar BRS Canará y CNPGL 93-41-1 y Wrockwona, presentan estimaciones de los parámetros cinéticos de producción de gas similares y mejores a los de los cultivares Camerún y Napier.

The aim of this study was to evaluate mathematical models comparing the kinetic parameters in the production of in vitro gas in different genotypes of elephant grass The design was entirely randomized with three repetitions with five treatments that were the elephant grass genotypes (BRS Canará, Napier, Vruckwona, Cameroon and CNPGL 93-41-1) with age of re-growth at 45 days. The ruminal inoculum was obtained from two whole male Nelores oxen kept in pickets formed by Urochloa brizantha cv. Marandu in the dry period of the year. The incubations were consecutive, measuring the gas production pressure readings in 2; 4; 6; 8; 10; 12; 19; 24; 36; 48; 60; 72; 84 and 96 hours. The determination of gas production kinetics was tested in two non-linear models: Monomolecular and Logistic. Where the final volume (Vf/mL), average gas production rate (k/h-1), production lage time (Lag/h) and average digestion time (MDT/h) were estimated. The models tested, the Logistic with latency showed better fit for all crop profiles. The BRS Canará and CNPGL 93-41-1 and Wrockwona cultivars present estimates of kinetic parameters of gas production similar and better than those of Cameroon and Napier cultivars.

Objetivou-se neste trabalho avaliar modelos matemáticos comparando os parâmetros cinéticos na produção de gás in vitro em diferentes genótipos de capim-elefante. O delineamento foi inteiramente casualizados com três repetições com cincos tratamentos que foram os genótipos de capim-elefante (BRS Canará, Napier, Vruckwona, Cameroon e CNPGL 93-41-1) com idade de maturidade de rebrota aos 45 dias. O inóculo ruminal foi obtido através de dois bois Nelores machos inteiros mantidos em piquetes formados por Urochloa brizantha cv. Marandu, no período seco do ano. As incubações foram consecutivas mensurando as leituras de pressão de produção de gás realizadas em 2; 4; 6; 8; 10; 12; 19; 24; 36; 48; 60; 72; 84 e 96 horas. A determinação da cinética de produção de gases foi testada dois modelos não lineares que são: Monomolecular e o Logístico. Onde foram estimados o volume final ( /mL), taxa média de produção de gás ( /h-1), o tempo de latência de produção ( /h) e o tempo médio de digestão (TMD/h-1). Os modelos testados, o Logístico com latência apresentou melhor ajuste para todos os perfis das cultivares. A cultivar BRS Canará e CNPGL 93-41-1 e Wrockwona, apresentam estimativas dos parâmetros cinéticos de produção de gás semelhantes e melhores aos comparados das cultivares Cameroon e Napier.

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Two studies were carried out to evaluate the effects of levels of soybean meal produced from damaged soybean grains replacing normal soybean meal on in vitro and in situ digestibility of ruminant diets as well as on intake, digestibility, and animal performance of growing/finishing lambs. In trial 1, we evaluated the in vitro digestibility of each soybean meals (normal and damaged), as well as diets containing levels of the damaged replacing the normal (0, 333, 667 and 1,000 g.kg−1) soybean meal on kinetic parameters of in vitro cumulative gas production, in vitro dry matter and crude protein digestibility, in situ rumen-degradable protein, rumen-undegradable protein, and in vitro intestinal digestibility of rumen-undegradable protein. In trial 2, we used 48 growing/finishing lambs to evaluate the effects of damaged soybean meal levels (0, 333, 667, and 1,000 g.kg−1) replacing normal soybean meal in feedlot diets (20:80 roughage:concentrate ratio) on intake and digestibility of nutrients and on animal performance. The damaged soybean meal presented lower values for total gas production and in vitro dry matter digestibility than normal soybean meal. Higher rumen-undegradable protein was estimated for damaged soybean meal than for the normal and consequently lower rumen-degradable protein for damaged compared to normal. Because of the lower rumen-degradable protein, damaged soybean meal promoted lower in vitro ammonium nitrogen (NH3 -N) concentrations than the normal in feedlot diets. In the in vivo trial, there were no effects of damaged soybean meal levels in the diets on intake and digestibility of nutrients (dry matter, organic matter, crude protein, and fiber) as well as on total weight gain, average daily gain, carcass yield, or feeding efficiency. Thus, damaged soybean meal can fully replace the normal one in lamb feedlot diets (in up to of 1,000 g.kg−1 of the normal soybean meal) without causing adverse effects on intake and digestibility of nutrients and on animal performance.

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The objective of this study was to evaluate increasing levels of inclusion of dry distillery grains (DDGs) in substitution of corn and urea in multiple supplements for beef cattle of Urochloa brizantha cv. Marandu on the consumption, digestibility, and efficiency of microbial synthesis and use of nitrogen. We used four Nellore bulls cannulated in the rumen with an average age of 24 months and 445.12 ± 34.4 kg of body weight (BW), in a 4 × 4 Latin square design. Supplements differed by the inclusion level of DDG (0%, 31.5%, 63.0%, and 94.5%). Increasing levels of DDG inclusion decreased dry matter intake (DMI) (P = 0.002), forage (P = 0.002), organic matter (OM) (P = 0.001), crude protein (CP) (P = 0.037), and total digestible nutrients (TDN) (P < 0.001) and had a quadratic effect on the intake of non-fibrous carbohydrates (NFC) (P = 0.002). It was observed an increase in the digestibility of ether extract (EE) (P = 0.005), however a decrease in the digestibility of NFC (P = 0.001). Inclusion of DDG did not influence ruminal pH. There was a quadratic effect at collection times for ruminal ammoniacal nitrogen (P < 0.05), except for the supplement with 94.5% DDG where the effect was linear (P = 0.002). Nitrogen intake was 10.9% higher when there was no DDG in the supplement, compared with the supplement with 94.5% DDG (P = 0.039). The excretion of N by feces was greater when there was a greater amount of DDG in the supplement (P = 0.027), the opposite occurred with urine excretion of N, being higher when there was less amount of DDG in the supplement (P = 0.027). Increasing levels of DDG did not affect ruminal microbial protein yield (P > 0.05). Replacing corn and urea with up to 94.5% DDGs in multiple supplements resulted in no adverse effect efficiency of microbial synthesis, although nutrient intake, total digestible nutrients, and use of nitrogen were reduced at 94.5% DDG inclusion.

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Our objective was to identify the best fit mathematical models for in vitro gas production kinetics using rumen fluid and forage plants commonly used in ruminant feed to obtain better estimates of parameters that describe the rumen fermentation. Four mathematical models were tested, two unicompartmental (M1 = first order, M2 = Gompertz) and two bicompartmental (M3 = M1 + M2; M4 = M2 + M2). Two temperate grasses were evaluated, as well as four tropical grasses and three temperate forage legumes. The fit of the models was verified by the corrected Akaike information criterion (AICcr) and the difference among AICcr values (Δr), likelihood probability (Wr), and relative likelihood (ERr). Temperate forages reached maximum gas production between 48 and 72 h. In the tropical forages, it occurred only after 72 h. In profiles in which M3 was the best choice, the values of parameters Vf 1 were higher than those of Vf 2, and k1 values were higher than k2 values. The only exception was for Tifton 85 profile, whose Vf 2 value was higher than Vf 1. The model M3 has a better fit for tropical forages with higher fiber content and lower levels of nonfibrous carbohydrates and crude protein. The model M1 has a better fit for forage with higher nonfibrous carbohydrate contents and low lignin content.(AU)

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As amostras de Clitoria ternatea L. foram cortadas aos 35, 50, 70 e 90 dias após o corte de uniformização na área experimental. O experimento foi projetado como um delineamento inteiramente casualizado, com total de 18 unidades experimentais (parcelas). A matéria seca (MS) e a produção de matéria seca (PMS) da planta inteira foram separadas quantitativamente em folhas, hastes e vagens para cada idade de corte. As análises bromatológicas e cinéticas de produção de gás in vitro realizadas avaliaram a qualidade de cada parte da planta. A produção, composição química e os parâmetros de produção de gás estimados foram analisados por meio de ajuste de um modelo estatístico misto com dois tipos de estrutura de covariância: componente de variância e não estruturada com variância heterogênea. Os ?pools? de produção rápida e lenta de gás foram detectados tanto para folhas quanto nas hastes. Apenas nas vagens foram detectados ?pool? simples de produção de gás. Pressuposta homocedasticidade foi observada para todas as variáveis exceto a cutina nas hastes e para alguns parâmetros da cinética de produção de gás das folhas e hastes. A produção de matéria seca da planta apresentou tendência cúbica semelhante a um padrão de curva sigmoide. A PMS de folhas apresentou efeito quadrático e a PMS de hastes aumentou linearmente. Não houve presença representativa de vagens aos 35 e 50 dias. O teor de MS da planta inteira teve efeito quadrático e apresentou o mínimo no intervalo de 90 dias. As frações não fibrosas e a cutina das folhas não foram afetadas pela idade de corte. A idade de corte afetou a maioria dos componentes químicos e os parâmetros de produção de gás relativos às hastes. Baseado no máximo da PMS para as folhas, o corte de 70 dias maximizou a quantidade de partes comestíveis de massa de leguminosas forrageiras de alta qualidade. No entanto, o equilíbrio entre produção sustentável de forragem e qualidade, tem que ser pesquisada.

Samples of Clitoria ternatea L. were harvested at 35, 50, 70, and 90 d after a uniformity harvest in a field study designed as a completely randomized design with a total of 18 experimental units. The dry matter (DM) yield (DMY) of the whole plant was separated quantitatively into leaves, stems, and pods at each harvesting age. Chemical analyses and in vitro gas production kinetics were performed to assess the quality of the plant parts. Yields, chemical composition, and estimates of gas production parameters were analyzed by fitting a mixed statistical model with two types of covariance structures as follows: variance components and an unrestricted structure with heterogeneous variances. Fast and slow gas yielding pools were detected for both leaves and stems parts, but only a single pool was detected for pods. The homocedasticity assumption was more likely for all variables, except leaf cutin, and for some parameters of the gas production kinetics of leaves and stems. The DMY of the whole plant presented a cubic trend and resembled a crescent sigmoid-shaped pattern. The leaf DMY presented a quadratic fit, and the stem DMY increased linearly. There was no presence of typical pods at 35 and 50 d. The DM content of the whole plant was quadratic and presented a minimum in the 90 d interval. The non-fibrous fractions and cutin of the leaves were unaffected by harvesting age, while the fibrous fractions were affected by harvesting age. The harvesting age affected the majority of the chemical constituents and gas kinetic parameters related to the stems. Based on the maximum DMY for leaves, the 70 d harvest maximized the amount of high quality edible parts of the forage legume mass. However, a compromise between a sustainable forage production and quality has to be achieved.

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