RESUMEN
Vancomycin (Vm) currently represents the gold standard against methicillin-resistant Staphylococcus aureus (MRSA) infections. However, it is associated with low oral bioavailability, formulation stability issues, and severe side effects upon systemic administration. These drawbacks could be overcome by Vm topical administration if properly encapsulated in a nanocarrier. Intriguingly, nanobubbles (NBs) are responsive to physical external stimuli such as ultrasound (US), promoting drug delivery. In this work, perfluoropentane (PFP)-cored NBs were loaded with Vm by coupling to the outer dextran sulfate shell. Vm-loaded NBs (VmLNBs) displayed â¼300nm sizes, anionic surfaces and good drug encapsulation efficiency. In vitro, VmLNBs showed prolonged drug release kinetics, not accompanied by cytotoxicity on human keratinocytes. Interestingly, VmLNBs were generally more effective than Vm alone in MRSA killing, with VmLNB antibacterial activity being more sustained over time as a result of prolonged drug release profile. Besides, VmLNBs were not internalized by staphylococci, opposite to Vm solution. Further US association promoted drug delivery from VmLNBs through an in vitro model of porcine skin. Taken together, these results support the hypothesis that proper Vm encapsulation in US-responsive NBs might be a promising strategy for the topical treatment of MRSA wound infections.
Asunto(s)
Antibacterianos , Preparaciones de Acción Retardada , Sistemas de Liberación de Medicamentos , Nanoestructuras , Vancomicina , Animales , Antibacterianos/administración & dosificación , Antibacterianos/química , Antibacterianos/efectos de la radiación , Línea Celular , Supervivencia Celular/efectos de los fármacos , Preparaciones de Acción Retardada/administración & dosificación , Preparaciones de Acción Retardada/química , Preparaciones de Acción Retardada/efectos de la radiación , Sulfato de Dextran/química , Composición de Medicamentos , Liberación de Fármacos , Estabilidad de Medicamentos , Fluorocarburos/química , Humanos , Técnicas In Vitro , Staphylococcus aureus Resistente a Meticilina/efectos de los fármacos , Staphylococcus aureus Resistente a Meticilina/crecimiento & desarrollo , Microscopía Electrónica de Transmisión , Nanoestructuras/administración & dosificación , Nanoestructuras/química , Nanoestructuras/efectos de la radiación , Nanoestructuras/ultraestructura , Piel/metabolismo , Absorción Cutánea , Porcinos , Ondas Ultrasónicas , Vancomicina/administración & dosificación , Vancomicina/química , Vancomicina/efectos de la radiaciónRESUMEN
The photocatalytic degradation of an antibiotic, vancomycin B hydrochloride (VAN-B), has been investigated in aqueous suspensions of titanium dioxide (TiO2) by monitoring the change in its concentration as well as the production of ammonia and chlorides as a function of irradiation time. The removal of 50mg L(-1) VAN-B solution yields maximum concentrations of 2.45 and 2.53 mg N-NH3 L(-1) after 120 min of photocatalytic oxidation using 0.1 and 0.2 g TiO2 L(-1), respectively. When 0.2 g TiO2 L(-1) were applied up to 87% of the stoichiometric amount of chloride was reached within 120 min of irradiation, corresponding to 0.087 mmol L(-1). A set ofbioassays (Daphnia magna, Pseudokirchneriella subcapitata and Ceriodaphnia dubia) was performed to evaluate the potential detoxification of VAN-B and its by-products of oxidation under chronic and acute tests. The toxicity of the treated VAN-B samples varied during the oxidation, due to the formation of some intermediate products more toxic than VAN-B. Despite almost total removal of VAN-B that was achieved within 120 min of irradiation, a significant increase in toxicity was observed in chronic tests proving that the chronic assays are more appropriate than acute ones to detect the impact of by-products formed during the photocatalytic degradation of antibiotics.
Asunto(s)
Fotólisis , Vancomicina/efectos de la radiación , Contaminantes Químicos del Agua/efectos de la radiación , Animales , Daphnia , Pruebas de Toxicidad Aguda , Pruebas de Toxicidad Crónica , Rayos Ultravioleta , Vancomicina/toxicidad , Contaminantes Químicos del Agua/toxicidadRESUMEN
Ionizing radiation can be used as a drug sterilization technique, provided that the drug itself is not modified and that no toxic products are produced; moreover, if the irradiated product is a drug delivery system, the drug release characteristics must not be significantly altered by radiation. The aim of this work was to study the effects of sterilization by ionizing radiation on hydroxyethylcellulose/trehalose spherical micromatrices, containing the antibiotic vancomycin. Our experimental results showed that gamma-rays did not alter the chromophore groups of vancomycin (UV measurements), and did not modify the kinetic behavior of drug release from microspheres. Moreover, no significant changes in the shape and in the size distribution of microspheres were found after irradiation. The electron spin resonance (ESR) spectroscopy was proven to be a valid identification method of the executed radiation treatment, even after 5 years. The experimental results showed that the therapeutic application of the pharmacological system investigated was not compromised by irradiation, and that ESR spectroscopy can be used to distinguish irradiated from non-irradiated products.
Asunto(s)
Celulosa/análogos & derivados , Celulosa/efectos de la radiación , Rayos gamma , Microesferas , Trehalosa/efectos de la radiación , Vancomicina/efectos de la radiación , Celulosa/química , Trehalosa/química , Vancomicina/químicaRESUMEN
Poly D,L-lactic acid (PLA) and its copolymers with glycolide PLGA 90:10 and 70:30 were polymerized under various conditions to yield polymers in the molecular weight range 12000-40000 daltons, as determined by gel permeation chromatography. Vancomycin hydrochloride was the hydrophilic drug of choice for the treatment of methicillin resistant Staphyloccoccal infections. It was microencapsulated in the synthesized polymers using water-oil-water (w/o/w) double emulsion and solvent evaporation. The influence of microcapsule preparation medium on product properties was investigated. An increase in polymer-to-drug ratio from 1:1 to 3:1 caused an increase in the encapsulation efficiency (i.e. from 44-97% with PLGA). An increase in the emulsifier (PVA) molecular weight from 14-72 kD caused an increase in encapsulation efficiency and microcapsule size. The in vitro release of vancomycin from microcapsules in phosphate buffer saline (pH 7.4) was found to be dependent on molecular weight and copolymer type. The kinetic behaviour was controlled by both diffusion and degradation. Sterilization with 60Co (2.5 Mrad) also affected the degradation rate and release profiles. Degradation of microcapsules could be seen by scanning electron microscopy, by the increase in the release rate from PLA and by the decrease in the Tg values of microcapsules. In vitro bactericidal effects of the microcapsule formulations on S. aureus were determined with a special diffusion cell after the preparations had been sterilized, and were found to have bactericidal effects lasting for 4 days.