RESUMEN
The influence of low-frequency electromagnetic (LF-EM) waves on microorganisms has been a subject of experimental investigations for more than two decades and the results are promising. In parallel, an interesting procedure known as biophysical-information-therapy or bioresonance therapy (BRT) which in principle is based on LF-EM stimulation, has emerged. BRT was discovered in the late 1980's but it is still poorly studied. This paper demonstrates that by transferring metronidazole information to water samples by an electronic amplifier (BRT device), the growth of axenically cultured trophozoites of Entamoeba histolytica and Trichomonasvaginalis is significantly inhibited, compared with those cultures treated with non and sham electro-transferred water samples. A positive control of metronidazole, a well-known cytotoxic drug against parasites, was used as a reference.
Asunto(s)
Antiprotozoarios/farmacología , Entamoeba histolytica/crecimiento & desarrollo , Metronidazol/farmacología , Fármacos Sensibilizantes a Radiaciones/farmacología , Trichomonas vaginalis/crecimiento & desarrollo , Agua/química , Bioensayo , Entamoeba histolytica/efectos de los fármacos , Entamoeba histolytica/efectos de la radiación , Radiación , Trichomonas vaginalis/efectos de los fármacos , Trichomonas vaginalis/efectos de la radiación , Agua/farmacologíaRESUMEN
Following cultivation in Asami medium, centrifugation and resuspension in saline or in water from a medicinal spring, Trichomonas vaginalis trophozoites were exposed to well-defined doses of ultraviolet (UV) light (254 nm). We used 24- and 48-h-old trichomonads at concentrations of 1 x 10(5) and 5 x 10(4) trophozoites/ml in a total volume of 20 ml for these studies. The apparatus for UV irradiation was especially constructed for batch experiments. After irradiation at doses ranging from 80 to 160 mJ/cm2, the mobility of the parasites was reduced and morphological alterations appeared: rounding of the cells, vacuolization of the cytoplasm and even cytolysis. A dose of 401.7 mJ/cm2 killed 99.8% of the 48-h-old trichomonads when irradiation occurred in saline at a cell density of 1 x 10(5) trichomonads/ml and 98.9% when irradiation was done at a cell density of 5 x 10(4) trichomonads/ml. A dose of 362.1 mJ/cm2 killed only the more sensitive 24-h-old trichomonads. In mineral water, 241 mJ/cm2 was sufficient to kill up to 99.5% of the 48-h-old trichomonads. When 48-h-old trichomonads that had been exposed to a radiation dose of 160-240 mJ/cm2 were subcultured, they lost their ability to propagate. At a dose of 80 mJ/cm2, both the trichomonads that had been harvested during the log phase and the 48-h-old organisms suspended in mineral water lost their ability to propagate on subculture. These results indicate that 24-h-old trichomonads were more sensitive than 48-h-old organisms. Furthermore, the experiments demonstrated that a higher dose of UV radiation must be applied to T. vaginalis trophozoites than to the more sensitive bacterial strain Escherichia coli ATCC 11229 so as to achieve comparable killing results.
Asunto(s)
Trichomonas vaginalis/efectos de la radiación , Rayos Ultravioleta , Animales , Relación Dosis-Respuesta en la Radiación , Aguas Minerales/análisisRESUMEN
1. The effects of continuous gamma radiation on the viability of Trichomonas vaginalis (ATCC 30001) were assessed by a colony count technique. 2. A triphasic survival curve showed an initial shoulder (Dq) of 3 Gy followed by three linear curves with D0 values of 34, 300, and 90 Gy. 3. Sterilization of 10(6) cells/ml occurred from 1600 to 1800 Gy of radiation. 4. Population growth, subsequent to radiation exposure of 17-100 Gy, showed an increased lag time followed by a faster rate of growth, compared with unirradiated cells. 5. Trichomonas vaginalis is more sensitive to ionizing radiation than free-living protozoa and appears as radiosensitive as those parasitic protozoa examined in radioattenuation experiments.