RESUMEN
Hydrogen sulfide (H2 S) is an important endogenous gasotransmitter, but the targeted delivery and real-time feedback of exogenous H2 S are still challenging. With the aid of density functional theory (DFT) calculations, we designed a new 1,3-dithiolium-4-olate (DTO) compound, which can react with a strained alkyne via the 1,3-dipolar cycloaddition and the retro-Diels-Alder reaction to generate carbonyl sulfide (COS) as the precursor of H2 S, and a thiophene derivative with turn-on fluorescence. Moreover, the diphenylamino substituent in DTO greatly increases the mitochondrial targeting of this H2 S delivery system. Such a bioorthogonal click-and-release reaction has integrated three functions in one system for the first time: (1)â in situ controllable H2 S release, (2)â concomitant fluorescence response, and (3)â mitochondria-targeted delivery. In addition, we investigated the mitochondrial membrane potential loss alleviation by using this system in H9c2 cells under oxidative stress.
Asunto(s)
Desarrollo de Medicamentos , Sulfuro de Hidrógeno/metabolismo , Mitocondrias/metabolismo , Tolueno/análogos & derivados , Teoría Funcional de la Densidad , Humanos , Sulfuro de Hidrógeno/química , Mitocondrias/química , Estructura Molecular , Tolueno/síntesis química , Tolueno/química , Tolueno/metabolismoRESUMEN
A series of novel substituted phenyl 1, 3-thiazolidin-4-one sulfonyl derivatives 5 (a-t) were synthesized and screened for their in-vitro anti-microbial and anti-viral activity. The result of the anti-microbial assay demonstrated compounds 5d, 5f, 5g, 5h, 5i, 5j showed prominent inhibitory activity against all the tested Gram-positive and Gram-negative bacterial strains, while compounds 5g, 5j, 5o, 5p, 5q showed significant activity against the entire set of fungal strains as compared to standard drug Ampicillin and Clotrimazole, respectively. The antimicrobial study revealed that compounds having electron-withdrawing groups showed significant antimicrobial potency. The most active antibacterial compound 5j showed potent inhibition of S. aureus DNA Gyrase enzyme as a possible mechanism of action for antimicrobial activity. Moreover, the antiviral testing of selected compounds showed considerable activity against Herpes simplex virus-1(KOS), Herpes simplex virus-2 (G), Herpes simplex virus-1(TK- KOS ACVr), Vaccinia virus, Human Coronavirus (229E), Reovirus-1, Sindbis virus, Coxsackie virus B4, Yellow Fever virus and Influenza A, B virus. Compounds 5h exhibited low anti-viral activity against HIV-1(strain IIIB) and HIV-2 (strain ROD). The study clearly outlined that synthesized compounds endowed with good antimicrobial property together with considerable antiviral activity.
Asunto(s)
Fenoles/síntesis química , Sulfonamidas/síntesis química , Tolueno/análogos & derivados , Animales , Antibacterianos/síntesis química , Antibacterianos/química , Antibacterianos/farmacología , Antivirales/síntesis química , Antivirales/química , Antivirales/farmacología , Bacterias/clasificación , Bacterias/efectos de los fármacos , Línea Celular , Chlorocebus aethiops , Humanos , Fenoles/química , Fenoles/farmacología , Sulfonamidas/química , Sulfonamidas/farmacología , Tolueno/síntesis química , Tolueno/química , Tolueno/farmacología , Células Vero , Virus/clasificación , Virus/efectos de los fármacosRESUMEN
To find potential inhibitors of human carbonic anhydrase IX (CAIX), we have successfully deigned, synthesized and characterized three p-toluene sulphonylhydrazone derivatives (1-3). Molecular docking studies provided the structural basis of CAIX inhibition and a deeper insight into the protein-ligand interactions. p-Toluene sulphonylhydrazone derivatives show a well organized conformational compatibility with the active site of CAIX. The protein-ligand complex was stabilized by several non-covalent interactions offered by residues present in the active site cavity. The actual binding affinity of synthesized compounds with CAIX was experimentally measured by fluorescence and isothermal titration calorimetry (ITC). Results of both fluorescence binding and ITC measurements show the binding affinity of p-Toluene sulphonylhydrazone derivatives to the CAIX in the µM range. CAIX enzyme inhibition assay showed the IC50 values in nM range. Though all the three compounds (1-3) showed a good binding with CAIX, compound 2 showed the best inhibition of CAIX activity. These compounds were non-toxic on normal cell lines (HEK-293) and significantly inhibit the proliferation of hypoxic cancer cells. All compounds induce apoptosis in the hypoxic cancer cells. These compounds may be further exploited as promising therapeutic agents to control the hypoxia-induced tumors.
Asunto(s)
Anhidrasa Carbónica IX/antagonistas & inhibidores , Inhibidores de Anhidrasa Carbónica/farmacología , Hidrazonas/química , Neoplasias/tratamiento farmacológico , Anhidrasa Carbónica IX/química , Inhibidores de Anhidrasa Carbónica/síntesis química , Inhibidores de Anhidrasa Carbónica/química , Hipoxia de la Célula/efectos de los fármacos , Línea Celular Tumoral , Proliferación Celular/efectos de los fármacos , Células HEK293 , Humanos , Hidrazonas/síntesis química , Hidrazonas/farmacología , Simulación del Acoplamiento Molecular , Neoplasias/enzimología , Tolueno/síntesis química , Tolueno/química , Tolueno/farmacología , Hipoxia Tumoral/efectos de los fármacosRESUMEN
In the classic Diels-Alder [4 + 2] cycloaddition reaction, the overall degree of unsaturation (or oxidation state) of the 4π (diene) and 2π (dienophile) pairs of reactants dictates the oxidation state of the newly formed six-membered carbocycle. For example, in the classic Diels-Alder reaction, butadiene and ethylene combine to produce cyclohexene. More recent developments include variants in which the number of hydrogen atoms in the reactant pair and in the resulting product is reduced by, for example, four in the tetradehydro-Diels-Alder (TDDA) and by six in the hexadehydro-Diels-Alder (HDDA) reactions. Any oxidation state higher than tetradehydro (that is, lacking more than four hydrogens) leads to the production of a reactive intermediate that is more highly oxidized than benzene. This increases the power of the overall process substantially, because trapping of the reactive intermediate can be used to increase the structural complexity of the final product in a controllable and versatile manner. Here we report an unprecedented overall 4π + 2π cycloaddition reaction that generates a different, highly reactive intermediate known as an α,3-dehydrotoluene. This species is in the same oxidation state as a benzyne. Like benzynes, α,3-dehydrotoluenes can be captured by various trapping agents to produce structurally diverse products that are complementary to those arising from the HDDA process. We call this new cycloisomerization process a pentadehydro-Diels-Alder (PDDA) reaction-a nomenclature chosen for chemical taxonomic reasons rather than mechanistic ones. In addition to alkynes, nitriles (RC≡N), although non-participants in aza-HDDA reactions, readily function as the 2π component in PDDA cyclizations to produce, via trapping of the α,3-(5-aza)dehydrotoluene intermediates, pyridine-containing products.
Asunto(s)
Reacción de Cicloadición , Hidrógeno/química , Tolueno/análogos & derivados , Benceno/química , Ciclización , Diinos/química , Hidrogenación , Isomerismo , Nitrilos/química , Oxidación-Reducción , Piridinas/química , Terminología como Asunto , Tolueno/síntesis química , Tolueno/químicaRESUMEN
The practical synthesis of the C-ring precursor of paclitaxel starting from 3-methoxytoluene is described. Lipase-catalyzed kinetic resolution of a substituted cyclohexane-1,2-diol, derived from 3-methoxytoluene in three steps, successfully afforded a desired enantiomer with >99% ee, which was transformed to a cyclohexenone. 1,4-Addition of a vinyl metal species, followed by Mukaiyama aldol reaction with formalin in the presence of a Lewis acid provided the known C-ring precursor of paclitaxel in a 10 g scale.
Asunto(s)
Antineoplásicos Fitogénicos/síntesis química , Paclitaxel/síntesis química , Tolueno/análogos & derivados , Tolueno/síntesis química , Ciclohexanos/química , Formaldehído/química , Lipasa/químicaRESUMEN
Drug therapy of seizures involves producing high levels of antiepileptic drugs in the blood. Drug must enter the brain by crossing from the blood into the brain tissue, called a transvascular route (TVR). Even before the drug can reach the brain tissue, factors such as systemic toxicity, macrophage phagocytises and reduction in oxygen content limit the success of this TVR. Encapsulating the drug within a nano scale delivering system, synthesising drugs with low molecular weight are the best mechanisms to deliver the drug to the brain. But through this article, we have explored a possibility of attaching a molecule 4-(trifluoromethyl) benzoic acid (TFMBA), that possess more number of fluorine atom, to benzodiazepine (BDZ) resulting in an ionic salt (S)-(+)-2,3-dihydro-1H-pyrrolo[2,1-c][1,4]benzodiazepine5,11(10H,11aH)-dione with 4-(trifluoromethyl)benzoic acid. By this way, reducing the toxicity of BDZ than the conventional anti-epileptic drugs (AEDs), increasing the solubility, reducing the melting point, enriching the TVR with excess oxygen content with the support of fluorine. With all these important prerequisites fulfilled, the drug along with the attached molecule is expected to travel more comfortably through the TVR without any external support than any other conventional AEDs. FTIR, (1)H NMR, (13)C NMR, HRMS spectroscopy, HRTEM and In vitro cytotoxicity analysis supports this study.
Asunto(s)
Anticonvulsivantes/química , Benzodiazepinonas/química , Pirroles/química , Tolueno/análogos & derivados , Anticonvulsivantes/síntesis química , Anticonvulsivantes/metabolismo , Anticonvulsivantes/toxicidad , Benzodiazepinonas/síntesis química , Benzodiazepinonas/toxicidad , Carbamazepina/toxicidad , Fructosa/análogos & derivados , Fructosa/toxicidad , Halogenación , Humanos , Células MCF-7 , Peso Molecular , Pirroles/síntesis química , Pirroles/toxicidad , Solubilidad , Estereoisomerismo , Tolueno/síntesis química , Tolueno/química , Tolueno/toxicidad , Topiramato , Temperatura de TransiciónRESUMEN
Reaction of p-toluenesulfonyl chloride with amino acids gave sulfonamides p-T1a-k which upon amidation afforded p-T2a-k. Similarly, treatment involving α-toluenesulfonyl chloride and amino acids afforded the sulfonamides α-T1a-k. These two classes of sulfonamides were synthetically modified at their COOH end position to achieve N,N-diethylamido substituted p-toluenesulfonamides p-T2a-k and α-toluenesulfonamides α-T2a-k, respectively. The chemical structures of the compounds were validated with IR, Mass spectra, NMR as well as elemental analytical data. Both classes of compounds were screened against Escherichia coli and Staphylococcus aureus and their activity werecompared. It was remarkable to note that the α-toluene sulfonamides α-T2a-k were more active than their p-toluenesulfonamide counterparts p-T2a-k. Compound 1-(benzylsulfonyl)-N,N-diethylpyrrolidine-2-carboxamide α-T2a was the most potent antibacterial compound on S. aureus with MIC value of 3.12 µg mL(-1) while N,N-Diethyl-3-phenyl-2-(phenylmethylsulfonamide) propanamide α-T2j emerged as the best antibacterial motif against E. coli with MIC value of 12.5 µg mL(-1). Hence, these compounds especially the α-toluenesulfonamide core structural templates are good candidates for further study for future drug discovery.
Asunto(s)
Antibacterianos/farmacología , Escherichia coli/efectos de los fármacos , Staphylococcus aureus/efectos de los fármacos , Sulfonamidas/farmacología , Tolueno/análogos & derivados , Antibacterianos/síntesis química , Descubrimiento de Drogas , Escherichia coli/crecimiento & desarrollo , Espectroscopía de Resonancia Magnética , Espectrometría de Masas , Pruebas de Sensibilidad Microbiana , Estructura Molecular , Espectrofotometría Infrarroja , Staphylococcus aureus/crecimiento & desarrollo , Relación Estructura-Actividad , Sulfonamidas/síntesis química , Tolueno/síntesis química , Tolueno/farmacologíaRESUMEN
We report the first example of the highly enantioselective synthesis of structurally diverse chiral dithioketals via asymmetric sulfenylation of various types of S-based nucleophiles, catalyzed by a cheap cinchona alkaloid derivative, dihydroquinine.
Asunto(s)
Quinidina/análogos & derivados , Ácidos Sulfínicos/química , Tolueno/análogos & derivados , Catálisis , Estructura Molecular , Quinidina/química , Tolueno/síntesis química , Tolueno/químicaRESUMEN
The current pharmacological Chagas disease treatments, using Nifurtimox or Benznidazole, show limited therapeutic results and are associated with potential side effects, like mutagenicity. Using random screening we have identified new chemotypes that were able to inhibit relevant targets of the Trypanosoma cruzi. We found 3H-[1,2]dithioles with the ability to inhibit Trypanosoma cruzi triosephosphate isomerase (TcTIM). Herein, we studied the structural modifications of this chemotype to analyze the influence of volume, lipophilicity and electronic properties in the anti-T. cruzi activity. Their selectivity to parasites vs. mammalian cells was also examined. To get insights into a possible mechanism of action, the inhibition of the enzymatic activity of TcTIM and cruzipain, using the isolated enzymes, and the inhibition of membrane sterol biosynthesis and excreted metabolites, using the whole parasite, were achieved. We found that this structural framework is interesting for the generation of innovative drugs for the treatment of Chagas disease.
Asunto(s)
Tolueno/análogos & derivados , Tripanocidas/química , Tripanocidas/farmacología , Trypanosoma cruzi/efectos de los fármacos , Animales , Línea Celular , Relación Dosis-Respuesta a Droga , Evaluación Preclínica de Medicamentos , Macrófagos/efectos de los fármacos , Ratones , Esteroles/antagonistas & inhibidores , Esteroles/biosíntesis , Tolueno/síntesis química , Tolueno/química , Tolueno/farmacología , Tripanocidas/síntesis química , Trypanosoma cruzi/metabolismoRESUMEN
The deprotection of a common precursor moiety in dithiolene chemistry was discovered to be fully reversible, which, besides being relevant for researchers working in very different fields with these non-innocent ligand systems, may even have an impact on CO2 housekeeping, as the deprotected ligand acts as an efficient trap.
Asunto(s)
Tolueno/análogos & derivados , Espectroscopía de Resonancia Magnética/métodos , Tolueno/síntesis química , Tolueno/metabolismoRESUMEN
Replacement reactions of toluene-3,4-dithiolatoarsenic(III) chloride with oxygen and sulphur donor ligands like benzoic acid, thiobenzoic acid, anhydrous sodium acetate, thioacetic acid, phenol, thiophenol, sodium salicylate and thio glycolic acid in 1:1 molar ratio as well as disodium oxalate in 2:1 molar ratio in refluxing anhydrous benzene yielded toluene-3,4-dithiolatoarsenic(III) mono oxo or thio carboxylic or phenolic derivatives of the general formula SC(6)H(3)(CH(3))SAsR {where R=OOCC(6)H(5), SOCC(6)H(5), OOCCH(3), SOCCH(3), OC(6)H(5), SC(6)H(5), OOCC(6)H(4)(OH), SCH(2)COOH} and SC(6)H(3)(CH(3))SAsOOC-COOAsS(CH(3))C(6)H(3)S. These synthesized derivatives are yellow, yellow-brown solids/ liquids and are soluble in common organic solvents like benzene, chloroform, dichloromethane, dimethyl formamide, dimethyl sulphoxide etc. These derivatives have been characterized by melting point determination, molecular weight determination, elemental analysis (C, H, S and As), spectral {UV, IR, NMR ((1)H and (13)C), ESI-Mass, SEM and powder X-ray diffraction} and thermal (TGA, DTA and DSC) studies. Some of these compounds have been screened for their antimicrobial activities using the disc diffusion method. These derivatives have shown good activity as antibacterial and antifungal agents on some selected bacterial and fungal strains, which increased on increasing the concentration. Chloroamphenicol and terbinafin were used as standards for the comparison.
Asunto(s)
Antibacterianos/síntesis química , Antibacterianos/farmacología , Arsenicales/química , Oxígeno/química , Azufre/química , Temperatura , Tolueno/síntesis química , Tolueno/farmacología , Antibacterianos/química , Bacterias/efectos de los fármacos , Rastreo Diferencial de Calorimetría , Electrones , Hongos/efectos de los fármacos , Ligandos , Espectroscopía de Resonancia Magnética , Pruebas de Sensibilidad Microbiana , Microscopía Electrónica de Rastreo , Polvos , Espectrometría de Masa por Ionización de Electrospray , Espectrofotometría Infrarroja , Termogravimetría , Tolueno/química , Difracción de Rayos XRESUMEN
Doubly radical: A novel entry to α,n-didehydrotoluene (DHT) diradicals is disclosed and proceeds through the photochemical activation of (chlorobenzyl)trimethylsilanes with chloride loss and elimination of the SiMe(3) (+) group. The products formed in solution are indicative of the intermediacy of the three isomers of the α,n-DHT.
Asunto(s)
Silanos/química , Tolueno/análogos & derivados , Tolueno/síntesis química , Alcadienos/química , Alquinos/química , Ciclización , Procesos Fotoquímicos , Fotoquímica/métodos , Tolueno/químicaRESUMEN
In this study, the molecular structure and spectroscopic properties of title compound were characterized by X-ray diffraction, FT-IR and UV-vis spectroscopies. These properties of title compound were also investigated from calculative point of view. The X-ray diffraction and FT-IR analyses reveal the existence of keto form in the solid state. UV-vis spectra were recorded in different organic solvents. The results show that title compound exists in both keto and enol forms in DMSO, EtOH but it exists in enol form in benzene. In addition, the title compound in DMSO showed new absorption band at 436 nm due to the high ionizing effect of this solvent. The geometry optimization of title compound in gas phase was performed using DFT method with B3LYP applying 6-311G(d,p) basis set. TD-DFT calculations starting from optimized geometry were carried out in gas phase to calculate excitation energies of title compound. The non-linear optical properties were computed with the same level of theory and title compound showed a good second order nonlinear optical property. In addition, thermodynamic properties were obtained in the range of 100-500 K.
Asunto(s)
Compuestos Azo/química , Colorantes/química , Ciclohexenos/química , Ciclohexenos/síntesis química , Tolueno/análogos & derivados , Compuestos Azo/síntesis química , Colorantes/síntesis química , Cristalografía por Rayos X , Isomerismo , Modelos Moleculares , Teoría Cuántica , Análisis Espectral , Tolueno/síntesis química , Tolueno/químicaRESUMEN
BF(3)·OEt(2)-catalyzed direct cyanation of indoles and pyrroles using a less toxic, bench-stable, and easily handled electrophilic cyanating agent N-cyano-N-phenyl-para-toluenesulfonamide (NCTS) affords 3-cyanoindoles and 2-cyanopyrroles in good yields with excellent regioselectivity. The substrate scope is broad with respect to indoles and pyrroles.
Asunto(s)
Indoles/síntesis química , Ácidos de Lewis/química , Nitrilos/química , Nitrilos/síntesis química , Pirroles/síntesis química , Sulfonamidas/química , Sulfonamidas/síntesis química , Tolueno/análogos & derivados , Catálisis , Indoles/química , Estructura Molecular , Pirroles/química , Estereoisomerismo , Tolueno/síntesis química , Tolueno/químicaRESUMEN
Pd-catalyzed highly para-selective C-H arylation of monosubstituted arenes (including toluene) is developed for the first time using an F(+) reagent as a bystanding oxidant. This finding provides a new retrosynthetic disconnection for para-substituted biaryl synthesis via C-H/C-H cross-coupling.
Asunto(s)
Hidrocarburos Aromáticos/síntesis química , Paladio/química , Catálisis , Técnicas de Química Sintética/métodos , Hidrocarburos Aromáticos/química , Oxidantes/química , Tolueno/síntesis química , Tolueno/químicaRESUMEN
Ponatinib (AP24534) was previously identified as a pan-BCR-ABL inhibitor that potently inhibits the T315I gatekeeper mutant, and has advanced into clinical development for the treatment of refractory or resistant CML. In this study, we explored a novel series of five and six membered monocycles as alternate hinge-binding templates to replace the 6,5-fused imidazopyridazine core of ponatinib. Like ponatinib, these monocycles are tethered to pendant toluanilides via an ethynyl linker. Several compounds in this series displayed excellent in vitro potency against both native BCR-ABL and the T315I mutant. Notably, a subset of inhibitors exhibited desirable PK and were orally active in a mouse model of T315I-driven CML.
Asunto(s)
Alquinos/síntesis química , Alquinos/farmacología , Compuestos de Anilina/síntesis química , Proteínas de Fusión bcr-abl/antagonistas & inhibidores , Tolueno/síntesis química , Administración Oral , Alquinos/química , Compuestos de Anilina/química , Compuestos de Anilina/farmacología , Animales , Ciclización , Modelos Animales de Enfermedad , Proteínas de Fusión bcr-abl/genética , Leucemia Mielógena Crónica BCR-ABL Positiva/tratamiento farmacológico , Ratones , Modelos Moleculares , Estructura Molecular , Mutación , Ratas , Relación Estructura-Actividad , Tolueno/química , Tolueno/farmacologíaRESUMEN
Since activation of p53 in response to cytotoxic stress may have proapoptotic or protective effects depending on the nature of the injury, inhibitors of p53 may have therapeutic interest as modulators of chemotherapy toxicity or efficacy. In an attempt to identify novel p53 inhibitors, a quality collection of compounds structurally related to pifithrin-ß were designed and synthesized as potential inhibitors of p53. The biochemical and biological evaluations supported that compounds of the tetrahydrobenzothiazole series were inhibitors of the p53 transcriptional activity and were effective in enhancing paclitaxel-induced apoptosis. In contrast, in spite of the increased cytotoxic potency, selected compounds of the benzothiazole series were not able to modulate the transcriptional activity of p53, as indicated by lack of change of p21 expression. The therapeutic interest of the compounds of the former series in combination with taxanes was confirmed in a human tumor xenograft model.
Asunto(s)
Antineoplásicos , Benzotiazoles/síntesis química , Benzotiazoles/farmacología , Neoplasias Ováricas/tratamiento farmacológico , Tolueno/análogos & derivados , Proteína p53 Supresora de Tumor/antagonistas & inhibidores , Animales , Antineoplásicos/síntesis química , Antineoplásicos/química , Antineoplásicos/farmacología , Benzotiazoles/química , Línea Celular Tumoral , Modelos Animales de Enfermedad , Femenino , Regulación de la Expresión Génica/efectos de los fármacos , Humanos , Concentración 50 Inhibidora , Tolueno/síntesis química , Tolueno/química , Tolueno/farmacología , Trasplante HeterólogoRESUMEN
A scalable synthesis of a potent renin inhibitor (1) is described. The absolute stereochemistry is set via an unprecedented diastereoselective Dieckmann cyclization directed by a remote chiral protecting group. This transformation enables preparation of chiral 1,3-[3.3.1]-diazabicyclononenes by desymmetrization of alkyl-esters, with selectivities ranging from 4 to 17:1.
Asunto(s)
Compuestos Azo/síntesis química , Compuestos Bicíclicos Heterocíclicos con Puentes/síntesis química , Inhibidores de Proteasas/síntesis química , Inhibidores de Proteasas/farmacología , Renina/antagonistas & inhibidores , Tolueno/análogos & derivados , Compuestos Azo/química , Compuestos Bicíclicos Heterocíclicos con Puentes/química , Cristalografía por Rayos X , Ciclización , Conformación Molecular , Estructura Molecular , Inhibidores de Proteasas/química , Estereoisomerismo , Tolueno/síntesis química , Tolueno/química , Tolueno/farmacologíaRESUMEN
A total of eight CF(3)-substituted phenylacetic and mandelic acids are shown to undergo efficient photodecarboxylation (PDC; Phi = 0.37-0.74) in basic aqueous solution to give the corresponding trifluoromethyltoluenes or trifluoromethylbenzyl alcohols. The products are consistent with the almost exclusive formation of benzylic carbanions that subsequently react with water, with minor amounts (< or = 5%) of radical-derived products detected. Quenching studies indicate that the reaction likely proceeds from the singlet excited state. This work demonstrates that the CF(3) group greatly facilitates the excited state ionic PDC of phenylacetic acids.
Asunto(s)
Hidrocarburos Fluorados/química , Ácidos Mandélicos/química , Fenilacetatos/química , Alcoholes Bencílicos/síntesis química , Alcoholes Bencílicos/química , Descarboxilación , Estructura Molecular , Fotoquímica , Teoría Cuántica , Estereoisomerismo , Tolueno/análogos & derivados , Tolueno/síntesis química , Tolueno/química , Rayos UltravioletaRESUMEN
A new process is described for preparing very pure linear alkanethiols and linear alpha,omega-alkanedithiols using a sequential alkylation of the title compound, followed by a ring closure to quantitatively give the corresponding 3-methyl[1,3]thiazolo[3,2-a]-[3,1]benzimidazol-9-ium salt and the alkanethiol derivative under mild conditions. The alkanethiol and the heteroaromatic salt are easily separated by a simple extraction process. The intermediate thiazolium quaternary salts resulting from the first reaction step can be isolated in quantitative yields, affording an odourless protected form of the thiols.