RESUMEN
Iron deficiency and visceral leishmaniasis are serious problems of public health. The aim of this study was to evaluate the effect of iron deficiency, induced by the iron chelator desferrioxamine, on the course of the infection by Leishmania chagasi in BALB/c mice. Our data show that the iron chelator caused significant reduction in hemoglobin concentration of treated mice and reduction in parasite load in spleen and liver. Significant differences were not observed in the production of IFN-gamma and IL-4 among the experimental groups. In conclusion, the data reported in this paper suggest that iron deficiency may favor the host. If there is not enough iron available to the parasite, its multiplication may be reduced and infection attenuated.
Asunto(s)
Anemia Ferropénica/complicaciones , Leishmania infantum , Leishmaniasis Visceral/complicaciones , Anemia Ferropénica/inducido químicamente , Animales , Citocinas/biosíntesis , Deferoxamina/toxicidad , Femenino , Hemoglobinas/efectos de los fármacos , Hemoglobinas/metabolismo , Leishmania infantum/efectos de los fármacos , Leishmania infantum/crecimiento & desarrollo , Leishmaniasis Visceral/parasitología , Leishmaniasis Visceral/patología , Hígado/efectos de los fármacos , Hígado/parasitología , Hígado/patología , Ratones , Ratones Endogámicos BALB C , Distribución Aleatoria , Sideróforos/toxicidad , Bazo/efectos de los fármacos , Bazo/parasitología , Bazo/patologíaRESUMEN
Pseudomonas fluorescens PAB strain produced pyoverdine in a synthetic medium, this pigment was purified by solvent extraction and ion exchange, then sterilized by filtration. Where cytotoxic effect on human leukocytes was assayed, death and lysis was observed. Sublytic doses decreased leukocytes phagocytosis and chemotaxis. Bacteria grew and produced pigment in blood stored a 4 degrees C, with a pyoverdine production of 0.13 mg/ml of serum after 5 days of incubation.