RESUMEN
The use of pyrethroids has increased over recent years, and corresponds to a higher exposure of animals to pesticide residues in the environment and diet. Here, an outbreak of pyrethroid poisoning in beef cattle was reported occurring in Midwestern Brazil. After veterinary evaluation, it was observed that the bovines presented common pyrethroid intoxication symptoms. Aiming to identify the cattle poising by pyrethroid, earwax samples were collected from two groups: exposed and nonexposed animals from the same farm. Blind earwax analyses of the bovines were carried out using headspace/gas chromatography-mass spectrometry (HS/GC-MS). The HS/GC-MS analysis detected the presence of bifenthrin in the earwax analysis of the exposed animals, confirmed by the comparison of its MS fragments with a bifenthrin standard, and also by its retention time relative to the internal standard. In summary, HS/GC-MS analysis of earwax emerges as a tool that can be used in the detection and monitoring of bifenthrin poisoning in cattle, as a useful veterinary diagnosis that ensures animal health and the safety of their products.
Asunto(s)
Cerumen/química , Cromatografía de Gases y Espectrometría de Masas , Residuos de Plaguicidas/análisis , Intoxicación , Piretrinas/análisis , Animales , Bovinos , Cromatografía de Gases y Espectrometría de Masas/métodos , Cromatografía de Gases y Espectrometría de Masas/veterinaria , Residuos de Plaguicidas/envenenamiento , Intoxicación/diagnóstico , Intoxicación/veterinaria , Piretrinas/envenenamientoRESUMEN
During an avian mass mortality event investigation at the National Fish and Wildlife Forensic Laboratory in Ashland, OR, imidacloprid became an insecticide of concern. A qualitative analytical toxicology screen of seeds, plucks (tongue, esophagus, and trachea), and ventricular contents was requested. A method for the extraction and qualitative analysis of the insecticide in animal tissues was therefore developed. The procedure relies on a combined Food Emergency Response Network (FERN) and QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) approach to sample extraction followed by qualitative analysis by gas chromatography-tandem mass spectrometry. Since imidacloprid is not amenable to the conditions of gas chromatography, a trimethylsilyl derivative was created and characterized. Proposed mechanisms for the creation of this derivative and its mass spectrum are described. The imidacloprid-trimethylsilyl (TMS) derivative was detected in all samples submitted.
Asunto(s)
Aves , Toxicología Forense/métodos , Neonicotinoides/análisis , Nitrocompuestos/análisis , Patología Veterinaria/métodos , Residuos de Plaguicidas/análisis , Animales , Autopsia , Enfermedades de las Aves/mortalidad , Toxicología Forense/instrumentación , Cromatografía de Gases y Espectrometría de Masas/veterinaria , Neonicotinoides/envenenamiento , Nitrocompuestos/envenenamiento , Patología Veterinaria/instrumentación , Residuos de Plaguicidas/envenenamiento , Intoxicación/mortalidad , Intoxicación/veterinaria , Espectrometría de Masas en Tándem/veterinariaRESUMEN
Little information is available regarding the adverse effects of pesticides on natural honey bee populations. This study highlights the detrimental effects of pesticides on honey bee olfaction through behavioural studies, scanning electron microscopic imaging of antennal sensillae and confocal microscopic studies of honey bee brains for calcium ions on Apis cerana, a native Indian honey bee species. There was a significant decrease in proboscis extension response and biologically active free calcium ions and adverse changes in antennal sensillae in pesticide exposed field honey bee populations compared to morphometrically similar honey bees sampled from low/no pesticide sites. Controlled laboratory experiments corroborated these findings. This study reports for the first time the changes in antennal sensillae, expression of Calpain 1(an important calcium binding protein) and resting state free calcium in brains of honey bees exposed to pesticide stress.
Asunto(s)
Abejas/efectos de los fármacos , Abejas/fisiología , Exposición a Riesgos Ambientales/efectos adversos , Residuos de Plaguicidas/envenenamiento , Olfato/efectos de los fármacos , Olfato/fisiología , Animales , Relación Dosis-Respuesta a Droga , India , Análisis Espacio-TemporalAsunto(s)
Enfermedad Hepática Inducida por Sustancias y Drogas/etiología , Contaminación de Alimentos , Enfermedades Transmitidas por los Alimentos , Frutas/efectos adversos , Fungicidas Industriales/envenenamiento , Residuos de Plaguicidas/envenenamiento , Rosaceae , Humanos , Ivermectina/análogos & derivados , Ivermectina/envenenamiento , Masculino , Persona de Mediana Edad , Piretrinas/envenenamiento , Triazoles/envenenamientoRESUMEN
Studies of the effects of environmental exposures on domestic and wild animals can corroborate or inform epidemiologic studies in humans. Animals may be sensitive indicators of environmental hazards and provide an early warning system for public health intervention, as exemplified by the iconic canary in the coal mine. This article illustrates the application of animal sentinel research to elucidate the effects of exposure to traditional and emerging contaminants on human health. Focusing on environmental issues at the forefront of current public health research, the article describes exposures to community air pollution, environmental tobacco smoke, and pesticides and associations with cancer, reproductive outcomes, and infectious diseases. Finally, it covers the role of marine mammals in monitoring the health of the oceans and humans.
Asunto(s)
Monitoreo del Ambiente/métodos , Contaminantes Ambientales/análisis , Modelos Animales , Residuos de Plaguicidas/análisis , Vigilancia de Guardia , Animales , Animales Domésticos , Animales Salvajes , Contaminantes Ambientales/envenenamiento , Humanos , Biología Marina , Residuos de Plaguicidas/envenenamientoRESUMEN
The incidence of fatal poisoning of birds of prey caused by carbofuran has increased markedly in Hungary since 2007. An experimental model with broiler chickens was used to study clinical signs of sublethal carbofuran poisoning in birds and to measure the residue concentrations of carbamate in tissues after exposure. Eight chickens were treated with a carbofuran-containing insecticide orally by gastric tube at a single dose of 2.5 mg/kg body weight, and clinical signs of poisoning were observed. Gas chromatography was used to determine carbofuran concentrations in the blood, muscle, and liver samples, and in stomach contents. Poisoning was characterized by typical muscarinic and nicotinic clinical signs without mortality. Carbofuran in the stomach and edible tissues of acutely poisoned birds may lead to secondary poisoning of predators and may also present risks to human health.
Asunto(s)
Enfermedades de las Aves/inducido químicamente , Carbofurano/envenenamiento , Insecticidas/envenenamiento , Residuos de Plaguicidas/análisis , Animales , Animales Salvajes , Aves , Carbofurano/análisis , Pollos , Femenino , Contenido Digestivo/química , Humanos , Hungría , Hígado/química , Masculino , Modelos Animales , Residuos de Plaguicidas/metabolismo , Residuos de Plaguicidas/envenenamiento , Intoxicación/veterinariaRESUMEN
From 1994 to 1999 in the Lower Fraser Valley region of southwest Canada, fonofos (Dyfonate G) was recommended for control of introduced wireworm (Agriotes spp.) pests on potato and other root crops. As part of a wildlife-monitoring program, we collected 15 raptors, including 12 bald eagles (Haliaeetus leucocephalus), found dead or debilitated on or near agricultural lands with severely inhibited brain and/or plasma cholinesterase activity and fonofos residues in ingesta. Bird remains, in nine cases waterfowl, were identified in the ingesta samples. Another seven bald eagles had severe cholinesterase inhibition, but without evidence of fonofos residues. During two winters from 1996 to 1998, 420 ha of potato fields, half of which had been treated the previous spring with fonofos and the remainder untreated, were searched weekly for evidence of wildlife mortality. Search efficiency was assessed with placed duck carcasses. Waterfowl outnumbered other species in field-use counts and comprised the greatest proportion of birds found dead. We found 211 wildlife remains, most scavenged; 35 intact carcasses were suitable for postmortem examination and/or toxicology analyses. Cholinesterase activity was assayed in brains of 18 waterfowl, five of which had severely depressed activity (average inhibition 74%; range, 69-78%). The gastrointestinal tract of a mallard found in a field treated with granular product contained 49 microg/g fonofos residues, linking waterfowl mortality with labelled use of the product. These findings demonstrate the risk of both primary and secondary poisoning by anticholinesterase insecticides where wildlife make intensive use of farmed fields.
Asunto(s)
Anseriformes , Enfermedades de las Aves/inducido químicamente , Fonofos/envenenamiento , Residuos de Plaguicidas/envenenamiento , Rapaces , Animales , Canadá , Colinesterasas/sangre , Escarabajos/efectos de los fármacos , Contaminantes Ambientales/análisis , Contaminantes Ambientales/envenenamiento , Femenino , Fonofos/análisis , Contenido Digestivo/química , MasculinoRESUMEN
DDT is a persistent insecticide that was widely used in the world from the 1940s until the 70s, when it was banned in the United States and other countries. Most of its toxic effects are not observed in the acute forms, but particularly after chronic exposure. These long-term issues include reproductive effects, varying according to the time of life in which the individuals were exposed. The aims of the current study were to review the principal toxicological effects of DDT on reproduction, stratifying by physiological periods of exposure, and based on the magnitude of these effects, to discuss the cost-benefit relationship of reintroducing DDT with the specifically defined vector control criteria.
O DDT é um inseticida persistente que foi amplamente utilizado no mundo a partir da década de 40 até a de 70, quando foi banido nos Estados Unidos e outros países. A maioria dos efeitos de sua toxicidade não é observável em formas agudas, mas especialmente após exposições crônicas. Dentre estes aspectos em longo prazo estão os efeitos reprodutivos, que variam de acordo com o a época da vida em que as pessoas foram expostas. Os objetivos deste estudo são: revisar os principais efeitos toxicológicos do DDT na reprodução, estratificando de acordo com os períodos fisiológicos de exposição; e a partir da magnitude destes efeitos discutir o custo-benefício da volta da utilização do DDT com critérios definidos de eliminação de vetores da malária.
Asunto(s)
Control de Mosquitos/métodos , DDT , Insectos Vectores/parasitología , Exposición Materna , Malaria/prevención & control , Exposición a Plaguicidas , Salud Pública , Brasil , Análisis Costo-Beneficio , Enfermedades Endémicas/prevención & control , Malaria/economía , Residuos de Plaguicidas/envenenamiento , Control de Vectores de las EnfermedadesRESUMEN
In 2006, the World Health Organization issued a position statement promoting the use of indoor residual spraying (IRS) with dichlorodiphenyltrichloroethane (DDT) for malaria vector control in epidemic and endemic areas. Other international organizations concurred because of the great burden of malaria and the relative ineffectiveness of current treatment and control strategies. Although the Stockholm Convention of 2001 targeted DDT as 1 of 12 persistent organic pollutants for phase-out and eventual elimination, it allowed a provision for its continued indoor use for disease vector control. Although DDT is a low-cost antimalarial tool, the possible adverse human health and environmental effects of exposure through IRS must be carefully weighed against the benefits to malaria control. This article discusses the controversy surrounding the use of DDT for IRS; its effective implementation in Africa; recommendations for deployment today, and training, monitoring, and research needs for effective and sustainable implementation. We consider the costs and cost effectiveness of IRS with DDT, alternative insecticides to DDT, and the importance of integrated vector control if toxicity, resistance, and other issues restrict its use.
Asunto(s)
DDT , Insectos Vectores/parasitología , Malaria/prevención & control , Control de Mosquitos/métodos , África , Animales , DDT/química , DDT/economía , DDT/envenenamiento , Enfermedades Endémicas/prevención & control , Vivienda , Humanos , Resistencia a los Insecticidas , Malaria/parasitología , Residuos de Plaguicidas/envenenamientoRESUMEN
Only scarce data are available on chronic copper poisoning in general toxicology literature. This paper reports four patients with chronic copper poisoning and one patient with acute poisoning. The cases with chronic poisoning in our study consisted of four members of a farmer family presenting to the emergency department (ED) with malaise, weakness, abdominal pain, headache, dizziness, tightness in the chest, leg and back pain, accompanied by significant anemia (hemoglobin [Hb]: 8.7 - 9.5 g/dl). They were hospitalized and investigated thoroughly, although there were no other findings or clues enlightening the etiology of anemia. The anemia was attributed to chronic copper exposure acquired from vegetables containing copper. The diagnosis was established by ruling out other possible etiologies and history coupled with laboratory findings. The patients were discharged with the recommendation on diet to avoid consumption of pesticide-treated vegetables. Their Hb values were between 10 and 11.4 g/dl on the 15th day, and between 12 and 14 g/dl after two months. Their symptoms had also resolved completely in two months. The patient with acute intoxication (5th case) had ingested copper oxychloride with suicidal intent. He was admitted with anuria and hemolytic anemia. After being hospitalized for fifteen days, he was diagnosed with chronic renal failure and was scheduled for a dialysis program. Acute poisoning is more deliberate, while chronic exposure may result in atypical findings. In conclusion, physicians working in primary care and EDs should consider copper poisoning in patients presenting with anemia, abdominal pain, headache, tightness in the chest, and leg and back pain.
Asunto(s)
Cobre/envenenamiento , Intoxicación/diagnóstico , Intoxicación/terapia , Enfermedad Aguda , Adulto , Anemia Hemolítica/inducido químicamente , Enfermedad Crónica , Servicio de Urgencia en Hospital , Femenino , Humanos , Fallo Renal Crónico/inducido químicamente , Fallo Renal Crónico/terapia , Masculino , Persona de Mediana Edad , Residuos de Plaguicidas/envenenamiento , Diálisis Renal , Intento de SuicidioRESUMEN
Three members of the same family ingested vegetables treated with aldicarb. All three developed signs and symptoms of acetylcholinesterase inhibition and all recovered a few hours after the ingestion. Reports of toxicity from the ingestion of aldicarb-contaminated food are uncommon. Aldicarb is a potent pesticide which can only be used safely if governmental and industry regulations are followed carefully.
Asunto(s)
Aldicarb/envenenamiento , Brassica , Contaminación de Alimentos , Insecticidas/envenenamiento , Residuos de Plaguicidas/envenenamiento , Adulto , Atropina/uso terapéutico , Brasil , Colinesterasas/sangre , Colinesterasas/metabolismo , Femenino , Humanos , Masculino , Persona de Mediana Edad , Antagonistas Muscarínicos/uso terapéutico , Intoxicación/tratamiento farmacológico , Intoxicación/metabolismo , Intoxicación/patologíaRESUMEN
The United Kingdom Wildlife Incident Investigation Scheme (WIIS) investigates cases of suspected poisoning of wildlife, honey bees, and companion animals by pesticides. Together with field inquiries and veterinary post-mortem examinations, the analytical procedures presented here provide a comprehensive approach to the investigation of these cases. The paper covers selection of animal tissues for analysis and methods suitable for the analysis of honey bees and for various types of bait. Seven multiresidue methods cover around 130 pesticides, and methods are also described for a further 8 compounds. These methods are currently used on samples submitted to the Scheme in England and Wales.
Asunto(s)
Técnicas de Química Analítica/métodos , Cromatografía de Gases/métodos , Cromatografía Liquida/métodos , Cromatografía/métodos , Contaminantes Ambientales/envenenamiento , Espectrometría de Masas/métodos , Plaguicidas/envenenamiento , Aluminio/química , Animales , Animales Salvajes , Anticoagulantes/farmacología , Abejas , Carbamatos/análisis , Cromatografía de Gases/instrumentación , Cromatografía en Gel , Cromatografía Liquida/instrumentación , Contaminantes Ambientales/análisis , Molleja No Aviar/efectos de los fármacos , Isoxazoles , Hígado/efectos de los fármacos , Espectrometría de Masas/instrumentación , Organofosfatos/toxicidad , Organofosfonatos/química , Residuos de Plaguicidas/análisis , Residuos de Plaguicidas/envenenamiento , Intoxicación/diagnóstico , Intoxicación/veterinaria , Gel de Sílice , Dióxido de Silicio/química , Estómago/efectos de los fármacos , Tetrazoles , Reino UnidoAsunto(s)
Atropina/uso terapéutico , Enfermedades Transmitidas por los Alimentos/tratamiento farmacológico , Plaguicidas/envenenamiento , Adolescente , Adulto , Femenino , Humanos , Insecticidas/envenenamiento , Masculino , Antagonistas Muscarínicos/uso terapéutico , Compuestos Organofosforados , Residuos de Plaguicidas/envenenamiento , Resultado del TratamientoRESUMEN
Because of the rapid decline of the endangered European mink (Mustela lutreola) populations in France, a national conservation program has been put into action, including research to understand the causes of decline. As part of this research, concentrations of eight anticoagulant rodenticides were examined in livers from 122 carcasses of four species of free-ranging mustelids collected between 1990 and 2002 in southwestern France. Bromadiolone residue was found in all species and 9% of the sample (one of 31 European mink, three of 47 American mink [Mustela vison], five of 33 polecats [Mustela putorius], and two of 11 European otters [Lutra lutra]). Liver concentrations ranged from 0.6 mug/g to 9.0 mug/g. Chlorophacinone residue was found in two species and 4% of the sample (in four of the American mink and in one of the otters), with liver concentrations ranging from 3.4 mug/g to 8.5 mug/g. Two polecats and one American mink had lesions and liver residues indicating bromadiolone was directly responsible for their death. However, most of our study animals survived secondary poisoning until they were caught; this study certainly underestimates the extent of fatal exposure of mustelids to rodenticides. Moreover, anticoagulant poisoning could increase their vulnerability to other causes of death. The current status of the endangered European mink population is such that any additional risk factor for mortality is important, and it is thus urgent to monitor and reduce the extensive use of bromadiolone and chlorophacinone against field rodents in France.
Asunto(s)
Hurones , Hígado/química , Visón , Nutrias , Residuos de Plaguicidas/envenenamiento , Rodenticidas/envenenamiento , Animales , Animales Salvajes , Causas de Muerte , Conservación de los Recursos Naturales , Monitoreo del Ambiente , Femenino , Francia , Masculino , Rodenticidas/administración & dosificaciónRESUMEN
The paper presents current trends in the use of pesticides and detection of their residues in the environmental objects. Pesticides are shown to be a serious health risk factor for different groups of the population when they are irrationally used and inadequately stored. A package of measures to prevent intoxication and to reduce a risk for pesticide entry into the body is provided.
Asunto(s)
Enfermedades de los Trabajadores Agrícolas/inducido químicamente , Agroquímicos/efectos adversos , Contaminación Ambiental , Exposición Profesional/efectos adversos , Residuos de Plaguicidas/efectos adversos , Plaguicidas/efectos adversos , Adulto , Agroquímicos/envenenamiento , Animales , Química Agrícola , Niño , Contaminación Ambiental/prevención & control , Femenino , Humanos , Masculino , Residuos de Plaguicidas/envenenamiento , Plaguicidas/envenenamiento , Medición de Riesgo , Factores de Riesgo , Federación de Rusia , Factores de TiempoRESUMEN
Toxaphene (camphechlor) was intensively used in the cotton growing fields of Nicaragua for decades with application rates as high as 31 kg ha(-1) in 1985. Although the use of this compound has recently been discontinued in the country, its intensive use in the past and its long persistence in soil allowed for the build up of large reservoirs of toxaphene in agriculture soils and a wide dispersal of residues in the environment. Measurements of toxaphene in coastal areas on the coast of the Pacific Ocean show that environmental concentrations are particularly high in the district of Chinandega, the traditional cotton growing region. Toxaphene residues measured in soils attained 44 microg g(-1) (dry weight) while concentrations in lagoon sediments attained 6.9 microg g(-1) (dry weight) near the mouth of the rivers flowing across the agricultural region. Measurements in aquatic biota showed concentrations as high as 1.6 microg g(-1) (dry weight) in the soft tissues of clams. The toxaphene reservoir in soils combined with the obvious persistence of this compound in soils and lagoon sediments allows predicting that toxaphene will remain in the coastal ecosystem at relatively high concentrations for many years. Toxic effects in lagoon fauna are likely to be observed especially in benthic species that may recycle this compound from sediments. Consumption of seafood, in particular of clams (Anadara spp.) from the more contaminated areas, may expose the population to unacceptably high intake of toxaphene, 30 microg d(-1) per person, with the diet.