RESUMEN
Maize is a crop of global economic importance and is widely cultivated throughout the Brazilian territory. The use of biostimulants can increase yield and improve crop yield. Unmanned aerial vehicles can be employed in arable areas, allowing their use in an economically way. This study to evaluate the use of biostimulant and the best application timing using photogrammetric indexes in maize, and indicate the most suitable plant index for yield increase through a Pearson's correlation. The DJI Drone coupled with RGB camera was used, and the images were processed through the AgisoftPhotoscan® software to generate the orthomosaic, and the QGIS® software version 3.4.15 with GRASS was used to generate thematic maps with the classification of the indexes of vegetation (NGRDI, EXG, SAVI, TGI, GLI, RI). A matrix of Pearson correlation coefficients between the variables was also created, and the results were analyzed with the R software. In general, the products Pyroligneous Extract (PE) and the hormonal product (HP) were the best for the two seasons studied. However, the HP was the best product to mitigate plant water stress in the dry period. Application at phenological stage V3 showed the lowest growth in the rainy season and in application to the seeds in the dry season. Dose 4 of the pyroligneous extract increased productivity in the rainy season and level 3.4 for the hormone product. Among the indexes evaluated, only the SAVI index showed significant differences between the others and showed significance for productivity in the two periods.
Asunto(s)
Tecnología de Sensores Remotos , Estaciones del Año , Zea mays , Zea mays/crecimiento & desarrollo , Reguladores del Crecimiento de las Plantas/farmacologíaRESUMEN
Much work has been dedicated to the quest to determine the structure-activity relationship in synthetic brassinosteroid (BR) analogs. Recently, it has been reported that analogs with phenyl or benzoate groups in the alkyl chain present activities comparable to those shown by natural BRs, depending on the nature of the substituent in the aromatic ring. However, as it is well known that the activity depends on the structure of the whole molecule, in this work, we have synthesized a series of compounds with the same substituted benzoate in the alkyl chain and a hydroxyl group at C3. The main goal was to compare the activities with analogs with -OH at C2 and C3. Additionally, a molecular-docking study and molecular dynamics simulations were performed to establish a correlation between the experimental and theoretical results. The synthesis of eight new BR analogs was described. All the analogs were fully characterized by spectroscopical methods. The bioactivity of these analogs was assessed using the rice lamina inclination test (RLIT) and the inhibition of the root and hypocotyl elongation of Arabidopsis thaliana. The results of the RLIT indicate that at the lowest tested concentration (1 × 10-8 M), in the BR analogs in which the aromatic ring was substituted at the para position with methoxy, the I and CN substituents were more active than brassinolide (50-72%) and 2-3 times more active than those analogs in which the substituent group was F, Cl or Br atoms. However, at the highest concentrations, brassinolide was the most active compound, and the structure-activity relationship changed. On the other hand, the results of the A. thaliana root sensitivity assay show that brassinolide and the analogs with I and CN as substituents on the benzoyl group were the most active compounds. These results are in line with those obtained via the RLIT. A comparison of these results with those obtained for similar analogs that had a hydroxyl group at C2 indicates the importance of considering the whole structure. The molecular-docking results indicate that all the analogs adopted a brassinolide-like orientation, while the stabilizing effect of the benzoate group on the interactions with the receptor complex provided energy binding values ranging between -10.17 and -13.17 kcal mol-1, where the analog with a nitrile group was the compound that achieved better contact with the amino acids present in the active site.
Asunto(s)
Arabidopsis , Brasinoesteroides , Simulación del Acoplamiento Molecular , Brasinoesteroides/química , Brasinoesteroides/síntesis química , Arabidopsis/efectos de los fármacos , Arabidopsis/crecimiento & desarrollo , Relación Estructura-Actividad , Simulación de Dinámica Molecular , Raíces de Plantas/química , Raíces de Plantas/crecimiento & desarrollo , Oryza/crecimiento & desarrollo , Hipocótilo/crecimiento & desarrollo , Hipocótilo/efectos de los fármacos , Hipocótilo/química , Reguladores del Crecimiento de las Plantas/síntesis química , Reguladores del Crecimiento de las Plantas/química , Reguladores del Crecimiento de las Plantas/farmacología , Estructura MolecularRESUMEN
Date palm (Phoenix dactylifera( cv. Medjool is a significant plant, grown in Jordan. In vitro propagation gives operative resources for the significant propagation of date palms. Maximum callus induction was achieved from MS media supplemented with benzyl amino purine (BA) and naphthalene acetic acid (NAA). The highest plant regeneration was recorded on MS medium supplemented with dichlorophenoxyacetic acid (2,4-D) at 3.0 mg/L, and BA at 2.0 mg/L. A significant positive impact on shoot formation was recorded on MS medium supplemented with 1.0 mg/L BA with 0.5 to 1.5 mg/L NAA in both liquid and solid MS medium. To maintain survival and regrowth capacity, sucrose could be used for medium-term conservation at lower concentrations (0.1 - 0.2 M). In addition, sorbitol might be used at 0.1 M to maintain the quality of explants. The vitrification technique for long-term preservation was experimented. Embryogenic callus was used as explants for conservation. The survival as well as regrowth percentages of non-cryopreserved and cryopreserved tissue cultures were affected by their duration of treatment with the vitrification solution plant vitrification solution 2 (PVS2) and modified plant vitrification solution 2 (MPVS2). Results showed that using PVS2 for 60 minutes for cryopreserved calli was more effective than other treatments. After storage in liquid nitrogen, the highest survival rate (65%) and regrowth rate (40%) were achieved.
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Phoeniceae , Brotes de la Planta , Regeneración , Phoeniceae/fisiología , Phoeniceae/efectos de los fármacos , Regeneración/fisiología , Regeneración/efectos de los fármacos , Brotes de la Planta/fisiología , Brotes de la Planta/crecimiento & desarrollo , Brotes de la Planta/efectos de los fármacos , Medios de Cultivo , Reguladores del Crecimiento de las Plantas/farmacología , Criopreservación , Técnicas de Cultivo de TejidosRESUMEN
Brassinosteroids (BRs) are an important group of polyhydroxylated naturally occurring steroidal phytohormones found in the plant kingdom in extremely low amounts. Due to the low concentrations in which these compounds are found, much effort has been dedicated to synthesizing these compounds or their structural analogs using natural and abundant sterols. In this work, we report the synthesis of new brassinosteroid analogs obtained from hyodeoxycholic acid, with a 3,6 dioxo function, 24-Nor-22(S)-hydroxy side chain and p-substituted benzoate function at C-23. The plant growth activities of these compounds were evaluated by two different bioassays: rice lamina inclination test (RLIT) and BSI. The results show that BRs' analog with p-Br (compound 41f) in the aromatic ring was the most active at 1 × 10-8 M in the RLIT and BSI assays. These results are discussed in terms of the chemical structure and nature of benzoate substituents at the para position. Electron-withdrawing and size effects seems to be the most important factor in determining activities in the RLIT assay. These results could be useful to propose a new structural requirement for bioactivity in brassinosteroid analogs.
Asunto(s)
Benzoatos , Brasinoesteroides , Oryza , Brasinoesteroides/química , Brasinoesteroides/síntesis química , Oryza/crecimiento & desarrollo , Oryza/efectos de los fármacos , Oryza/metabolismo , Benzoatos/química , Benzoatos/farmacología , Benzoatos/síntesis química , Reguladores del Crecimiento de las Plantas/síntesis química , Reguladores del Crecimiento de las Plantas/química , Reguladores del Crecimiento de las Plantas/farmacología , Desarrollo de la Planta/efectos de los fármacos , Ácido DesoxicólicoRESUMEN
Despite being valuable for producing a natural sweetener Curculin, Curculigo latifolia has a low growth and difficult to domestificate. So, to solve this problem, propagation on in vitro culture will be an alternative method to propagated this spesies under different cytokinins and light condition. Cytokinins and light has major role in organogenesis, growth and gene expression of many species. Thus, in this study, we aimed to improve the Curculigo latifolia growth on in vitro condition and expression of curculin gene by combining cytokinins addition and different light exposure. Four weeks seedlings were sub-cultured into medium (MS free hormone) containing 3 mg/L benzyladenine (BA) and various concentrations of meta-Topolin (mT) including 0.1 mg/L, 0.5 mg/L, and 5 mg/L. The cultures then incubated under different light types (red, blue, white LED lights and white fluorescence light) with 16-h light/ 18-h dark photoperiod for 14 weeks at 25 ± 2°C. Several parameters, including plant height, leaf number, chlorophyll contents, stomatal structure, and density and curculin expression, were observed every week. Unexpectedly, our results showed that C. latifolia growth displayed significant improvement when it was treated under white LED light without any additional cytokinins. In sum, white LED light further improves plantlets phenotype, such as plant height, leaf number, chlorophyll production, and stomatal number and structure, whereas, red LED light lead to a decreased phenotypes but increase the curculin gene expression.
Asunto(s)
Curculigo , Citocininas , Luz , Reguladores del Crecimiento de las Plantas , Citocininas/farmacología , Curculigo/genética , Reguladores del Crecimiento de las Plantas/farmacología , Regulación de la Expresión Génica de las Plantas/efectos de los fármacosRESUMEN
Peas are an important agricultural crop of great importance in human and animal nutrition. Peas, being a legume crop, help replenish nitrogen reserves in the soil. In field studies of the Federal State Budgetary Scientific Institution of the Federal Scientific Center of Legumes and Goat Crops (Oryol region), the influence of various growth regulators and biological products on the yield and quality indicators of pea seeds of the Nord and Multik varieties was studied. Pea plants are grown on dark gray forest, medium loamy soil of average cultivation. Before sowing, pea seeds were treated with solutions of Kornevin, Albit and Epin-extra by soaking for 5 hours. Solutions of the drugs were used at a concentration of 10-6 M, then dried and treated with Rizotorfin before sowing. Growth rates during the growing season and the yield of pea plants were determined. The content of protein, starch and amylose in starch was determined in the seeds. Research results have shown that the yield of pea plants depends on weather conditions. Under favorable weather conditions, the highest yield was obtained from the pea variety Nord (42.2 c/ha) in the variant with seed treatment with Kornevin, and in the Multik variety (43.0 c/ha) when treated with Rizotorfin. In arid conditions, the highest yield of peas of the Nord variety was obtained using the preparations Epin-extra and Kornevin. The highest yield of peas of the Multik variety was obtained using the preparations Rizotorfin, Kornevin and Epin-Extra. The research results, confirmed by statistical evaluation, showed that bioregulators and growth regulators help stimulate the amount of nitrogen supplied to plants, as well as the synthetic processes of protein synthesis. This contributed to improving the quality of seeds and green mass.
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Pisum sativum , Reguladores del Crecimiento de las Plantas , Semillas , Pisum sativum/crecimiento & desarrollo , Pisum sativum/efectos de los fármacos , Semillas/crecimiento & desarrollo , Semillas/química , Semillas/efectos de los fármacos , Reguladores del Crecimiento de las Plantas/farmacología , Reguladores del Crecimiento de las Plantas/análisis , Estaciones del Año , Productos Biológicos/análisisRESUMEN
The production of seedlings of the passion fruit tree, usually, is sexual, and the seeds are not uniform in the seedling emergence, and soaking treatments of seeds can provide faster and more uniform germination. It was aimed to study the action of plant growth regulators and the mobilization of reserves in the stages of soaking of yellow passion fruit seeds. The seeds were soaked for five hours in solutions containing plant growth regulators, in a completely randomized design, in a factorial 8 x 4, with four replications. The first factor corresponds to eight plant growth regulators: T1 - distilled water (control); T2 - 6-benzylaminepurine ââ500 mg L-1; T3 - 4-(3-indolyl) butyric acid 500 mg L-1; T4 - gibberellic acid 500 mg L-1; T5 - spermine 250 mg L-1; T6 - spermine 750 mg L-1; T7 - spermidine 750 mg L-1; T8 - spermidine 1250 mg L-1; and the second factor, to the four soaking times: zero, four, 72 and 120 hours, corresponding, respectively, to the dry seed, and to phases I, II, and III of the imbibition curve. It was evaluated the biochemical composition of seeds (lipids, soluble sugars and starch). The seeds showed accumulation of lipids in phase III; the content of soluble sugars increased in phase I and decreased in phase II. The starch content increased until the phase II and decreased in phase III. Starch is the main reserve in the seeds and the main source of energy used in phase III; soaking the seeds in polyamines generates an accumulation of lipids in the seeds and soaking in plant growth regulators increases the burning of starch.
Asunto(s)
Passiflora , Reguladores del Crecimiento de las Plantas , Reguladores del Crecimiento de las Plantas/farmacología , Frutas , Espermidina , Espermina , Ácido Butírico , Plantones , Almidón , AzúcaresRESUMEN
Bioavailability of nutrients, the scarcity of synthetic fertilisers, and the rising cost of fuel have all contributed to an increase in production costs, which has in turn reduced crop productivity and led scientists to seek out new methods to ensure high-quality output. In this context, various cytokinins dosages were tested in Peru to see whether they affected the quality of caigua, in an effort to address these issues. To mitigate these problems, a pot experiment was carried out to check the effects of various doses of cytokinin in the quality of caigua in Peru. The experiment consisted of 5 treatments including (0, 50, 100, 150 and 200 mL of cytokinin) by using (Anthesis Plus per 200 L of water) as a source, each with three replicates and placed following a randomized complete block design (RCBD). Treatment with 100 mL of cytokinins foliar analysis resulted in a caigua length of 18.9 cm, an increase in diameter of 5.65 cm, and an improvement in pulp thickness of 7.60 millimeters. Physiological parameters of caigua plants taken after 45 days of sowing were considerably improved with the same treatment. Similarly, N, K and Zn concentration in leaf was higher in case of 100 mL of cytokinins foliar analysis. Therefore, policymakers must advise using the recommended quantity of cytokinins to bring about regime transition, and formers can gain by injecting 100 mL of cytokinins to boost production and the economy. It was concluded that the adequate dose of cytokinins is in treatment T3, which raised value of potassium concentration in leaves, this influenced optimal development, strengthening against environmental stress and therefore quality. For this reason, research was carried out on the comparative study of cytokinin doses in the quality of caigua in Peru; the objective was to determine the appropriate dose to obtain higher quality fruit. Likewise, it was underlined that the objective was to employ an ecological alternative of plant origin such as the usage of phytohormone that stimulates the growth of the plant and consequently the quality of the fruit. The obtained the results were served as a recommendation for farmers in the area.
Asunto(s)
Citocininas , Reguladores del Crecimiento de las Plantas , Citocininas/farmacología , Perú , Reguladores del Crecimiento de las Plantas/farmacología , Hojas de la Planta/fisiología , Estrés FisiológicoRESUMEN
Abscisic acid (ABA) is a classical hormone involved in the plant defense against abiotic stresses, especially drought. However, its role in the defense response against biotic stresses is controversial: it can induce resistance to some pathogens but can also increase the susceptibility to other pathogens. Information regarding the effect of ABA on the relationship between plants and sedentary phytonematodes, such as Meloidogyne paranaensis, is scarce. In this study, we found that ABA changed the susceptibility level of Arabidopsis thaliana against M. paranaensis. The population of M. paranaensis was reduced by 58.3% with the exogenous application of ABA 24 h before the nematode inoculation, which demonstrated that ABA plays an important role in the preinfectional defense of A. thaliana against M. paranaensis. The increase in the nematode population density in the ABA biosynthesis mutant, aba2-1, corroborated the results observed with the exogenous application of ABA. The phytohormone did not show nematicide or nematostatic effects on M. paranaensis juveniles in in vitro tests, indicating that the response is linked to intrinsic plant factors, which was corroborated by the decrease in the number of nematodes in the abi4-1 mutant. This reduction indicates that the gene expression regulation by transcript factors is possibly related to regulatory cascades mediated by ABA in the response of A. thaliana against M. paranaensis.
Asunto(s)
Proteínas de Arabidopsis , Arabidopsis , Tylenchoidea , Animales , Arabidopsis/genética , Arabidopsis/metabolismo , Ácido Abscísico/farmacología , Ácido Abscísico/metabolismo , Proteínas de Arabidopsis/genética , Proteínas de Arabidopsis/metabolismo , Proteínas de Arabidopsis/farmacología , Reguladores del Crecimiento de las Plantas/farmacología , Reguladores del Crecimiento de las Plantas/metabolismoRESUMEN
BACKGROUND: Cryopreservation currently represents the most suitable strategy for the long-term conservation of plant germplasm. While much effort has focused on the development of protocols to enable successful cryostorage, there are few, if any reports, that consider the effect of cryogenic temperatures on the phytohormone status of the seed and developing seedlings. OBJECTIVE: To investigate the effect of cryopreservation on external seed coat features as well as levels of indole-3-acetic acid (IAA), abscisic acid (ABA) and 1-aminocyclopropane-1-carboxylic acid (ACC) in maize. MATERIALS AND METHODS: Two groups of seeds at 6% moisture content were compared: one was maintained at 4 degree C (control) while the other was exposed to LN within cryo-vials. RESULTS: Seeds exposed to cryogenic temperatures were characterized by the presence of large cracks in the seed coat compared with control seeds. Cryogenic exposure also resulted in a reduction in biomass and plant height. Results from the phytohormone analysis showed an initial reduction in the levels of IAA, ABA and ACC after 7 days of growth followed by sharp increase in levels relative to the control by 14 days. Whilst the roles of ABA and ethylene (and by extension, its precursor ACC) are well studied as stress response molecules, much less is known about the potentially vital role of auxins in regulating plant growth under conditions of low temperature stress. CONCLUSION: It is postulated that the interaction of all three hormones modulate crosstalk between various stress responses and recovery pathways to ameliorate the damage caused by freezing stress and enable plant survival. Given the dearth of information on phytohormones in cryobiology, more studies are needed to fully elucidate these relationships in the context of freezing stress caused by liquid nitrogen. Doi.org/10.54680/fr23610110612.
Asunto(s)
Reguladores del Crecimiento de las Plantas , Zea mays , Reguladores del Crecimiento de las Plantas/farmacología , Reguladores del Crecimiento de las Plantas/metabolismo , Criopreservación , Ácido Abscísico/farmacología , Semillas/fisiología , NitrógenoRESUMEN
WRKY transcription factors (TFs) play key roles in plant defense responses through phytohormone signaling pathways. However, their functions in tropical fruit crops, especially in banana, remain largely unknown. Several WRKY genes from the model plants rice (OsWRKY45) and Arabidopsis (AtWRKY18, AtWRKY60, AtWRKY70) have shown to be attractive TFs for engineering disease resistance. In this study, we isolated four banana cDNAs (MaWRKY18, MaWRKY45, MaWRKY60, and MaWRKY70) with homology to these rice and ArabidopsisWRKY genes. The MaWRKY cDNAs were isolated from the wild banana Musa acuminata ssp. malaccensis, which is resistant to several diseases of this crop and is a progenitor of most banana cultivars. The deduced amino acid sequences of the four MaWRKY cDNAs revealed the presence of the conserved WRKY domain of ~60 amino acids and a zinc-finger motif at the N-terminus. Based on the number of WRKY repeats and the structure of the zinc-finger motif, MaWRKY18 and MaWRKY60 belong to group II of WRKY TFs, while MaWRKY45 and MaWRKY70 are members of group III. Their corresponding proteins were located in the nuclei of onion epidermal cells and were shown to be functional TFs in yeast cells. Moreover, expression analyses revealed that the majority of these MaWRKY genes were upregulated by salicylic acid (SA) or methyl jasmonate (MeJA) phytohormones, although the expression levels were relatively higher with MeJA treatment. The fact that most of these banana WRKY genes were upregulated by SA or MeJA, which are involved in systemic acquired resistance (SAR) or induced systemic resistance (ISR), respectively, make them interesting candidates for bioengineering broad-spectrum resistance in this crop.
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Arabidopsis , Musa , Musa/genética , Reguladores del Crecimiento de las Plantas/farmacología , Reguladores del Crecimiento de las Plantas/metabolismo , Factores de Transcripción/metabolismo , Regulación de la Expresión Génica de las Plantas , Proteínas de Plantas/metabolismo , Ácido Salicílico/farmacología , Arabidopsis/genética , Aminoácidos/genética , Zinc/metabolismoRESUMEN
Somatic embryogenesis is a natural phenomenon through which somatic embryos are produced from somatic cells although. It is considered the most efficient morphogenic pathways for plant multiplication. One of the key features of somatic embryogenesis is the use of cellular totipotency, where dedifferentiation is induced to foster cell proliferation, followed by the induction of differentiation using plant growth regulators to produce new plants. There is a cell group with the potential to undergo the somatic embryogenesis pathway through adequate stimulation (plant growth regulators, incubation conditions, and supplementation of the culture medium). There are two somatic embryogenesis pathways in plants: direct and indirect embryogenesis. Direct somatic embryogenesis consists of the formation of embryos directly from isolated cells, without the formation of "callous" tissue. Indirect somatic embryogenesis is characterized by the formation of a callus as a stage that precedes the formation of somatic embryos. It should be stressed that not all plant cells have this morphogenic capacity; consequently, determining the type of factors that drive this type of response has been challenging. This book provides the reader with updated available information on the techniques, relevant protocols, and tools to perform somatic embryogenesis in different plant species for economic purposes.
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Reguladores del Crecimiento de las Plantas , Técnicas de Embriogénesis Somática de Plantas , Medios de Cultivo , Desarrollo Embrionario/genética , Reguladores del Crecimiento de las Plantas/farmacología , Técnicas de Embriogénesis Somática de Plantas/métodos , Plantas/genéticaRESUMEN
Somatic embryogenesis is an in vitro plant morphogenetic process due to cell totipotentiality to induce shoot regeneration. To induce this proliferation pathway, we used auxins such as 2,4-dichlorophenoxyacetic acid in combination with cytokinins. There are numerous somatic embryogenesis protocols for a great diversity of plants, including orchids, but none has been yet reported in Vanilla planifolia. Vanilla (V. planifolia) is propagated mainly asexually through cuttings. Under in vitro conditions, it is propagated asexually through direct and indirect organogenesis involving the use of various plant growth regulators in different concentrations. The cell response depends on explant type, culture medium used, and incubation conditions. Direct organogenesis involves de novo formation from differentiated cells; the indirect pathway develops from cell dedifferentiation that produces an explant called "callus." In most cases, this type of cell regeneration uses Benzyladenine. The explants most used in this pathway are shoots, roots, and protocorms, although some studies report the use of other types of explants, including leaves and seeds. Somatic embryogenesis in vanilla has been poorly studied partly because of the recalcitrance of this species. This work mentioned the advances in the in vitro morphogenesis of V. planifolia, mentioning the advantages and disadvantages of each morphogenetic pathway and its characteristics.
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Vanilla , Desarrollo Embrionario , Ácidos Indolacéticos/metabolismo , Reguladores del Crecimiento de las Plantas/farmacología , Brotes de la PlantaRESUMEN
Somatic embryogenesis (SE) serves as a key biological model for studying cell totipotency and the ontogenic processes of zygotic embryogenesis in plants. The SE process, under in vitro conditions, can be induced from different sources of explant cultivated in a culture medium with plant growth regulators (PGR) or by subjecting tissues to abiotic stress treatments. Somatic embryogenesis, in plant tissue culture (PTC), is a multifactorial event. The use of PGR, particularly auxins, is an important factor during induction. However, in vitro abiotic stress treatments are physiologically, biochemically, and genetically relevant and should be further studied.
Asunto(s)
Técnicas de Embriogénesis Somática de Plantas , Plantas , Desarrollo Embrionario , Ácidos Indolacéticos , Reguladores del Crecimiento de las Plantas/farmacologíaRESUMEN
Plant Growth Promoting Rhizobacteria (PGPR) are beneficial bacteria that colonize plant roots and promote plant growth through a variety of mechanisms such as phosphate solubilization, phytohormones production, antifungal activity and also improve plant growth and yield. Field experiment was carried out to investigate the residual effect of organic amendments plus soil microbes along with integrated nutrient management. (PGPR) are important soil organism that promotes plant growth and yield root colonization is an example of a direct and indirect mechanism. The treatments included control, (inorganic fertilizer and no organic fertilization).Five bacterial strains were identified morphologically and biochemically screened from the rhizospheres of chickpea, lentil, barseem, mungbean, and sesame. The experiment was conducted at the Arid Zone Research Center in D.I.Khan (Pakistan). The majority of isolates resulted in significant increase in shoot length, root length, and dry matter production of Cicer arietinum seedlings' shoot and root. The experiment represented that isolates treated plots with rhizobium strain inoculation resulted in greater plant height (35.000 cm) and nodule count (38.00) No of pods per plant-1 (44.66) when compared to the control treatment, While (Mesorhizobium cicero) along with organic amendments showed significant response the greater root length (50 cm) was observed in T4 treatment. The Performance of rhizobial strains on chickpea germination in an arid environment was found to significantly increase crop germination percentage. This combination thus increases nitrogen and phosphorus uptake in inoculation treated plots. The study found that plots with inoculation treatments yielded significantly higher than non-treated plots Treatment with Mesorhizobium Cicero and compost resulted in a higher grain yield (8%) as compared to the control. The greater grain yield was observed in Treatment T4 (183.67).The result showed that use of PGPR have the potential to increase nutrient absorption from soil while improved growth of chickpea.
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Cicer , Cicer/microbiología , Fósforo , Reguladores del Crecimiento de las Plantas/farmacología , Rizosfera , Suelo/químicaRESUMEN
Extracts of Ascophyllum nodosum are commonly used as commercial biostimulants in crop production. To further understand the seaweed extract-induced phenomena in plants, a transcriptomic study was conducted. RNA-seq differential gene expression analysis of tomato plants treated with a commercial A. nodosum extract formulation (Stimplex) revealed the up-regulation of 635 and down-regulation of 456 genes. Ontology enrichment analysis showed three gene categories were augmented, including biological processes, cellular components, and molecular functions. KEGG pathway analysis revealed that the extract had a strong influence on the expression of genes involved in carbon fixation, secondary metabolism, MAPK-signalling, plant hormone signal transduction, glutathione metabolism, phenylpropanoid and stilbenoid metabolism, and plant-pathogen interactions. qRT-PCR validation analysis using 15 genes established a strong correlation with the RNA sequencing results. The activities of defence enzymes were also significantly enhanced by seaweed extract treatment. Furthermore, AN-SWE treated tomato plants had significantly higher chlorophyll and growth hormone content and showed improved plant growth parameters and nutrient profiles than the control. It is postulated that seaweed extract-induced gene regulation was responsible for favourable plant responses that enabled better growth and tolerance to stress conditions. This study provides evidence at the transcriptomic level for the positive effects of foliar application of the Ascophyllum nodosum extract (Stimplex) observed in treated tomato plants.
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Ascophyllum , Solanum lycopersicum , Solanum lycopersicum/genética , Extractos Vegetales/farmacología , Reguladores del Crecimiento de las Plantas/farmacología , TranscriptomaRESUMEN
Recent results suggest that metabolism-mediated stomatal closure mechanisms are important to regulate differentially the stomatal speediness between ferns and angiosperms. However, evidence directly linking mesophyll metabolism and the slower stomatal conductance (gs ) in ferns is missing. Here, we investigated the effect of exogenous application of abscisic acid (ABA), sucrose and mannitol on stomatal kinetics and carried out a metabolic fingerprinting analysis of ferns and angiosperms leaves harvested throughout a diel course. Fern stomata did not respond to ABA in the time period analysed. No differences in the relative decrease in gs was observed between ferns and the angiosperm following provision of sucrose or mannitol. However, ferns have slower gs responses to these compounds than angiosperms. Metabolomics analysis highlights that ferns have a higher accumulation of secondary rather than primary metabolites throughout the diel course, with the opposite being observed in angiosperms. Our results indicate that metabolism-mediated stomatal closure mechanisms underpin the differential stomatal speediness regulation among ferns and angiosperms, in which the slower stomatal closure in ferns is associated with the lack of ABA-responsiveness, to a reduced capacity to respond to mesophyll-derived sucrose and to a higher carbon allocation toward secondary metabolism, which likely modulates both photosynthesis-gs and growth-stress tolerance trade-offs.
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Ácido Abscísico/farmacología , Helechos/fisiología , Magnoliopsida/fisiología , Manitol/farmacología , Reguladores del Crecimiento de las Plantas/farmacología , Estomas de Plantas/fisiología , Sacarosa/farmacología , Helechos/metabolismo , Cinética , Magnoliopsida/metabolismoRESUMEN
Low temperature remarkably limits rubber tree (Hevea brasiliensis Muell. Arg.) growth, latex production, and geographical distribution, but the underlying mechanisms of Hevea brasiliensis cold stress response remain elusive. Here, we identified HbSnRK2.6 as a key component in ABA signaling functions in phytohormone abscisic acid (ABA)-regulated cold stress response in Hevea brasiliensis. Exogenous application of ABA enhances Hevea brasiliensis cold tolerance. Cold-regulated (COR) genes in the CBF pathway are upregulated by ABA. Transcript levels of all five HbSnRK2.6 members are significantly induced by cold, while HbSnRK2.6A, HbSnRK2.6B, and HbSnRK2.6C can be further activated by ABA under cold conditions. Additionally, HbSnRK2.6s are localized in the cytoplasm and nucleus, and can physically interact with HbICE2, a crucial positive regulator in the cold signaling pathway. Overexpression of HbSnRK2.6A or HbSnRK2.6B in Arabidopsis extensively enhances plant responses to ABA and expression of COR genes, leading to increased cold stress tolerance. Furthermore, HbSnRK2.6A and HbSnRK2.6B can promote transcriptional activity of HbICE2, thus, increasing the expression of HbCBF1. Taken together, we demonstrate that HbSnRK2.6s are involved in ABA-regulated cold stress response in Hevea brasiliensis by regulating transcriptional activity of HbICE2.
Asunto(s)
Ácido Abscísico/farmacología , Respuesta al Choque por Frío , Regulación de la Expresión Génica de las Plantas/efectos de los fármacos , Hevea/metabolismo , Proteínas de Plantas/metabolismo , Proteínas Quinasas/metabolismo , Factores de Transcripción/metabolismo , Hevea/efectos de los fármacos , Hevea/genética , Reguladores del Crecimiento de las Plantas/farmacología , Proteínas de Plantas/genética , Proteínas Quinasas/genética , Factores de Transcripción/genéticaRESUMEN
This review aims to elucidate the state of the art of microalgae-based biostimulants as a tool in agriculture by summarizing the biologically active compounds factors that influence the use of microalgae biostimulants and their application methods in the field. Additionally, we examined the factors that support the use of microalgal biostimulants to face abiotic and biotic stress in crop plants. The use of microalgae in crop production and the benefits of seed preparation, foliar application, soil drenching, and hydroponic treatments were discussed. Furthermore, the use of these biostimulants in crop plants and their multiple benefits such as, better rooting, higher crop, fruit yields, drought and salinity tolerance, photosynthetic activity and pathogen resistance was thoroughly presented. The present situation of microalgal biostimulants and their difficulties in the market was analyzed, as well as the perspectives of their use. However, data shows that microalgal derived biostimulants can be used as an alternative for the protection of crops and plant growth regulators and play a significant key role in increasing the levels of production, yield and health of crops. Special interest needs to focus on investigating more microalgae species and their biological active compound factors, due to the largely untapped field. Perspectives regarding future research lines and development priorities were included.
Asunto(s)
Factores Biológicos/farmacología , Productos Agrícolas/crecimiento & desarrollo , Microalgas/fisiología , Reguladores del Crecimiento de las Plantas/farmacología , Producción de Cultivos , Productos Agrícolas/efectos de los fármacos , Suelo , Estrés FisiológicoRESUMEN
KEY MESSAGE: The moss Pseudocrossidium replicatum is a desiccation-tolerant species that uses an inducible system to withstand severe abiotic stress in both protonemal and gametophore tissues. Desiccation tolerance (DT) is the ability of cells to recover from an air-dried state. Here, the moss Pseudocrossidium replicatum was identified as a fully desiccation-tolerant (FDT) species. Its gametophores rapidly lost more than 90% of their water content when exposed to a low-humidity atmosphere [23% relative humidity (RH)], but abscisic acid (ABA) pretreatment diminished the final water loss after equilibrium was reached. P. replicatum gametophores maintained good maximum photosystem II (PSII) efficiency (Fv/Fm) for up to two hours during slow dehydration; however, ABA pretreatment induced a faster decrease in the Fv/Fm. ABA also induced a faster recovery of the Fv/Fm after rehydration. Protein synthesis inhibitor treatment before dehydration hampered the recovery of the Fv/Fm when the gametophores were rehydrated after desiccation, suggesting the presence of an inducible protective mechanism that is activated in response to abiotic stress. This observation was also supported by accumulation of soluble sugars in gametophores exposed to ABA or NaCl. Exogenous ABA treatment delayed the germination of P. replicatum spores and induced morphological changes in protonemal cells that resembled brachycytes. Transcriptome analyses revealed the presence of an inducible molecular mechanism in P. replicatum protonemata that was activated in response to dehydration. This study is the first RNA-Seq study of the protonemal tissues of an FDT moss. Our results suggest that P. replicatum is an FDT moss equipped with an inducible molecular response that prepares this species for severe abiotic stress and that ABA plays an important role in this response.