RESUMEN

Interleukin-18 (IL-18), a pro-inflammatory cytokine belonging to the interleukin-1 (IL-1) family, is involved in the pathogenesis of autoimmune/autoinflammatory and allergic diseases such as juvenile idiopathic arthritis and bronchial asthma. IL-18 forms a signalling complex with the IL-18 receptor α (IL-18Rα) and ß (IL-18Rß) chains; however, the detailed activation mechanism remains unclear. Here, the IL-18-IL-18Rα binary and IL-18-IL-18Rα-IL-18Rß ternary complexes were purified and crystallized as well as IL-18 alone. An X-ray diffraction data set for IL-18 was collected to 2.33 Šresolution from a crystal belonging to space group P21, with unit-cell parameters a = 68.15, b = 79.51, c = 73.46 Å, ß = 100.97°. Crystals of both the IL-18 binary and ternary complexes belonging to the orthorhombic space groups P21212 and P212121, respectively, diffracted to 3.10 Šresolution. Unit-cell parameters were determined as a = 135.49, b = 174.81, c = 183.40 Šfor the binary complex and a = 72.56, b = 111.56, c = 134.57 Šfor the ternary complex.

Asunto(s)

Interleucina-18/química , Receptores de Interleucina-18/química , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Cromatografía en Gel , Cristalización , Cristalografía por Rayos X , Humanos , Interleucina-18/aislamiento & purificación , Datos de Secuencia Molecular , Unión Proteica , Receptores de Interleucina-18/aislamiento & purificación , Células Sf9 , Spodoptera