RESUMEN
Cystic ovaries (CO) characterize a disorder frequently found in dairy cattle. However, despite the contributions by several researchers, the mechanism that leads to ovulatory failure has not yet been completely elucidated. Thus, the aim of this study was to examine the mRNA expression of bovine vascular endothelial growth factor (VEGFA)-164, VEGFA-164b and VEGF receptors (VEGFR1 and VEGFR2) by real-time PCR and protein expression by immunohistochemistry, immunofluorescence and Western blot in follicular fluid from dairy cows with spontaneous CO and in an experimental model of follicular persistence induced by prolonged treatment with progesterone. Results showed that both VEGFA isoforms and receptors were coexpressed in granulosa and theca interna cells and in follicular fluid of ovaries from all the groups evaluated. VEGFA-164, VEGFA-164b and VEGFR2 protein expression was higher in theca cells of persistent follicles from group P0 (expected time of ovulation) than in those from dominant follicles (as reference structure) from the control group (pâ¯<â¯0.05). Also, VEGFA-164 expression was higher in theca cells of cysts than in those of dominant follicles of the control group (pâ¯<â¯0.05). In follicular fluid, VEGFA-164 expression was higher in persistent follicles from group P5 (5 days of follicular persistence) than in the control, P0 and P15 groups, and higher in cysts than in dominant follicles from the control group (pâ¯<â¯0.05). This study provides evidence of an altered expression of VEGFA-164, VEGFA-164b and VEGFR2 during the formation of persistent follicles and cysts in cows. Together, these results evidence that early development of CO in cows is concurrent with an altered expression of these growth factors and that these alterations may contribute to the follicular persistence, angiogenic dysregulation and ovulatory failure found in cows with follicular cysts.
Asunto(s)
Enfermedades de los Bovinos/genética , Enfermedades de los Bovinos/fisiopatología , Quistes Ováricos/genética , Quistes Ováricos/fisiopatología , Folículo Ovárico/fisiología , Factor A de Crecimiento Endotelial Vascular/fisiología , Animales , Estudios de Casos y Controles , Bovinos/fisiología , Enfermedades de los Bovinos/metabolismo , Femenino , Quiste Folicular/genética , Quiste Folicular/metabolismo , Quiste Folicular/fisiopatología , Expresión Génica , Quistes Ováricos/metabolismo , Ovario/metabolismo , Ovario/patología , Ovulación/genética , Ovulación/metabolismo , Receptores de Factores de Crecimiento Endotelial Vascular/metabolismo , Receptores de Factores de Crecimiento Endotelial Vascular/fisiología , Factor A de Crecimiento Endotelial Vascular/metabolismoRESUMEN
Apresentaçäo de três casos de comedöes familiares examinados no Hospital Universitário Antônio Pedro, Niterói, RJ, em 1992. Após uma revisäo literária, alguns dos aspectos mais interessantes säo discutidos