RESUMEN
We used solid phase indirect hemadsorption assay (SPIHA) to detect specific IgM antibodies in specimens from cerebrospinal fluid, pleural fluid and ascitic fluid in patients with tuberculosis and compared with the enzyme linked immunosorbent assay (ELISA) and indirect hemagglutination assay (IHA). The result showed that detection of tuberculous specific IgM antibodies in cerebrospinal fluid by SPIHA is important for diagnosing the tuberculous meningitis.
Asunto(s)
Anticuerpos Antibacterianos/análisis , Inmunoglobulina M/análisis , Mycobacterium tuberculosis/inmunología , Tuberculosis/inmunología , Ascitis/líquido cefalorraquídeo , Ascitis/inmunología , Pruebas de Inhibición de la Hemadsorción/métodos , Humanos , Derrame Pleural/líquido cefalorraquídeo , Derrame Pleural/inmunología , Tuberculosis/líquido cefalorraquídeoRESUMEN
A GM1 erythroassay (GERYDO) for heat-labile Escherichia coli enterotoxin (LT) and cholera toxin (CT) is described. This assay was developed for use in poorly equipped laboratories in developing countries. It uses GM1-coated polystyrene plates and is based on the competition between the toxin to be assayed and CT covalently bound to sheep red blood cells. GERYDO can detect 0.9 or 0.5 ng of CT per ml depending on the method of sensitization of erythrocytes. Good quantitative and qualitative correlation with the enzyme-linked immunosorbent assay and the Vero cell test was observed.
Asunto(s)
Toxinas Bacterianas/análisis , Enterotoxinas/análisis , Proteínas de Escherichia coli , Gangliósido G(M1)/metabolismo , Pruebas de Inhibición de la Hemadsorción/métodos , Estudios de Evaluación como Asunto , TemperaturaRESUMEN
An immune adherence hemadsorption test for the rapid group-specific identification of adenoviruses is described. The test was performed directly on the cell culture isolate in less than 1 h, by the sequential addition of the antiserum of any adenovirus serotype, complement, and then C3b receptor-rich human erythrocytes. Microscopic examination revealed the presence of erythrocytes adhering firmly to the infected cell sheet. The immune adherence hemadsorption test proved a specific and rapid diagnostic procedure for the detection of adenovirus group antigen in tissue cultures and was more simple than conventional tests.
Asunto(s)
Adenoviridae/inmunología , Antígenos Virales/análisis , Células Cultivadas , Pruebas de Inhibición de la Hemadsorción/métodos , Reacción de Inmunoadherencia/métodos , Microscopía ElectrónicaAsunto(s)
Hemabsorción/efectos de los fármacos , Interferones/análisis , Virus de la Parainfluenza 1 Humana/efectos de los fármacos , Línea Celular , Células Clonales , Conjuntiva , Fluoresceína-5-Isotiocianato , Fluoresceínas , Pruebas de Inhibición de la Hemadsorción/métodos , Humanos , Sueros Inmunes , Interferones/farmacología , Virus de la Parainfluenza 1 Humana/inmunología , Espectrometría de Fluorescencia/métodos , TiocianatosRESUMEN
A solid-phase hemadsorption (SPHA) technique for the rapid detection of Treponema pallidum-specific IgM antibodies is described. The demonstration of these immunoglobulins permits differentiation between a recent and a long-lasting infection and facilitates the decision as to whether or not a patient should receive therapy for syphilis. Errors that occur in the fluorescent treponemal antibody-absorbed test with IgM conjugate (IgM-FTA-Abs test) because of high titers of T. pallidum-specific IgG (competitive inhibition), rheumatoid factors, or 7S IgM do not occur with the SPHA technique. This test is easy to perform; it requires only microtiter plates coated with antisera to human IgM in addition to the standard T. pallidum hemagglutination reagents. As compared with the Veneral Disease Research Laboratory (vdrl) test, SPHA test has the advantage of being specific for T. pallidum; furthermore, IgM antibodies can be demonstrated earlier after infection and disappear sooner after adequate treatment of syphilis.