RESUMEN
Hydrophobic bioactives can be more easily incorporated into food and have their bioavailability enhanced if nanostructured lipid carriers (NLC) are used as carriers. In the present study, beta-carotene-loaded NLC were produced by low emulsification using murumuru butter and a mixture of Span 80 and Cremophor RH40 as surfactants. Their average diameter was 35 nm and alpha-tocopherol was required to protect the encapsulated ß-carotene. Besides the evaluation of their physicochemical stability, NLC were submitted to dynamic in vitro digestion and cell viability assays with Caco-2 and HEPG cells. The bioaccessibility of beta-carotene in the dynamic system was about 42%. Regarding cell viability, results indicated NLC were toxic to the cell cultures tested. Such high toxicity is probably related to the type of surfactant used and to the extremely reduced particle size, which may have led to an intense and fast permeation of the NLC through the cells.
Asunto(s)
Antioxidantes/administración & dosificación , Portadores de Fármacos/química , Lípidos/química , Provitaminas/administración & dosificación , alfa-Tocoferol/administración & dosificación , beta Caroteno/administración & dosificación , Antioxidantes/química , Células CACO-2 , Supervivencia Celular/efectos de los fármacos , Portadores de Fármacos/toxicidad , Combinación de Medicamentos , Humanos , Lípidos/toxicidad , Magnoliopsida/química , Nanoestructuras/química , Nanoestructuras/toxicidad , Transición de Fase , Provitaminas/química , Temperatura de Transición , alfa-Tocoferol/química , beta Caroteno/químicaRESUMEN
Provitamin A (proVA) enhanced maize was developed to help alleviate vitamin A deficiency in maize-consuming populations. Nixtamalization (lime-cooking process) is the most commonly used maize-preparation method in Mexico and Central America. In this study, the effect of traditional nixtamalization (TN) and nixtamalized extrusion (NE) on proVA retention was evaluated. Kernel conversion to TN dough led to high proVA apparent retention (>100%), while kernel conversion to NE flour led to lower retention (85%). However, TN tortilla proVA carotenoid concentration was similar to the kernels' original concentration and slightly higher in NE tortillas. Genotypic variation has a strong effect on proVA retention in TN dough and NE flour, but no such variation in proVA retention was observed in tortillas. Tortillas prepared with proVA-enhanced maize, using either TN or NE, are a good source of proVA carotenoids. Also, dough made using TN and proVA-enhanced maize is a high proVA-content ingredient for other food products.
Asunto(s)
Pan , Carotenoides/química , Manipulación de Alimentos/métodos , Alimentos Fortificados , Zea mays/química , Pan/análisis , Carotenoides/análisis , Genotipo , Humanos , México , Provitaminas/química , Vitamina A/análisis , Zea mays/genéticaRESUMEN
The present study was carried out to examine the effect of vitamin premix reduction or withdrawal in finisher diet (29 to 42 days of age) on the performance and meat quality of chicks fed a wheat/barley based diet and reared on floor pens. A total of 504 male broiler chicks (Ross® 308) were allocated to seven treatment groups, with four replicates per treatment and 18 birds per replicate. All data were analyzed according to a completely randomized design. Results showed that the reduction or withdrawal of the vitamin premix from diets in different weeks of finisher period did not affect chicken performance (p>0.05). There were no significant differences in TBARS values of thigh samples of birds slaughtered at 35 days of age. However, the TBARS value of the treatment without vitamin premix was significantly higher than in the other treatments (p < 0.05) when birds were slaughtered at 42 days of age. Finally, the results of this study demonstrated that it is not possible withdrawal all premix vitamin of the diet. However, it may be possible to reduce vitamin supplements in finisher broiler diets without any negative effects on performance or meat quality during freezing.(AU)