RESUMEN
OBJECTIVE: There was no study aimed at evaluating the effect of muscle function on SLE patients' quality of life using the Sarcopenia Quality of Life (SarQoL) questionnaire. METHODS: This cross-sectional study recruited 61 women with SLE consecutively, muscle function was measured with Jamar handheld-dynamometer and 6-meter walk test, HRQoL was measured with Sarcopenia Quality of Life (SarQoL) questionnaire. The cut-off point for low muscle strength (<18 kg) and low gait speed (<1.0 m/s) was according to the Asian Working Group on Sarcopenia 2019 criteria. Statistical analysis was conducted with a t-test for mean difference, and linear regression was used to adjust confounders (age, protein intake, physical exercise, and disease activity). RESULTS: The subjects' mean muscle strength was 19.54 kg (6.94), and 44.3% (n = 27) was found to have low muscle strength. The subjects' mean gait speed was 0.77 m/s (0.20), and 90.3% (n = 55) was found to have low gait speed. The difference of total SarQoL score in subjects with normal and low muscle strength was found to be significant; 74.86 (9.48) vs. 65.49 (15.51) (p = 0.009), and still statistically significant after adjustments with age, protein intake, physical exercise level, and disease activity [B 0.56; 95% CI 0.08-1.03; p = 0.022]. The difference of total SarQoL score in subjects with normal and low physical performance was found to be not significant, 70.67 (11.08) vs. 70.72 (13.56) (p = 0.993). CONCLUSION: There was a significant difference in SarQoL's total score in normal compared with low muscle strength groups of Indonesian women with SLE.
Asunto(s)
Lupus Eritematoso Sistémico/fisiopatología , Lupus Eritematoso Sistémico/psicología , Fuerza Muscular/fisiología , Músculos/fisiopatología , Adulto , Estudios Transversales , Ejercicio Físico/fisiología , Femenino , Humanos , Indonesia/epidemiología , Lupus Eritematoso Sistémico/diagnóstico , Fuerza Muscular/inmunología , Proteínas/administración & dosificación , Proteínas/provisión & distribución , Calidad de Vida/psicología , Sarcopenia/fisiopatología , Índice de Severidad de la Enfermedad , Encuestas y Cuestionarios , Prueba de Paso/métodos , Velocidad al Caminar/fisiologíaRESUMEN
AIMS: Iron deficiency (ID) is common in heart failure (HF) patients and negatively impacts symptoms and prognosis. The aetiology of ID in HF is largely unknown. We studied determinants and the biomarker profile of ID in a large international HF cohort. METHODS AND RESULTS: We studied 2357 worsening HF patients from the BIOSTAT-CHF cohort. ID was defined as transferrin saturation <20%. Univariable and multivariable logistic regression models were constructed to identify determinants for ID. We measured 92 cardiovascular markers (Olink Cardiovascular III) to establish a biomarker profile of ID. The primary endpoint was the composite of all-cause mortality and first HF rehospitalization. Mean age (±standard deviation) of all patients was 69 ± 12.0 years, 26.1% were female and median N-terminal pro B-type natriuretic peptide levels (+interquartile range) were 4305 (2360-8329) ng/L. Iron deficiency was present in 1453 patients (61.6%), with highest prevalence in females (71.1% vs. 58.3%; P < 0.001). Independent determinants of ID were female sex, lower estimated protein intake, higher heart rate, presence of peripheral oedema and orthopnoea, chronic kidney disease, lower haemoglobin, higher C-reactive protein levels, lower serum albumin levels, and P2Y12 inhibitor use (all P < 0.05). None of these determinants were sex-specific. The biomarker profile of ID largely consisted of pro-inflammatory markers, including paraoxonase 3 (PON3) and tartrate-resistant acid phosphatase type 5. In multivariable Cox proportional hazard regression analyses, ID was associated to worse outcome, independently of predictors of ID (hazard ratio 1.25, 95% confidence interval 1.06-1.46; P = 0.007). CONCLUSION: Our data suggest that the aetiology of ID in worsening HF is complex, multifactorial and seems to consist of a combination of reduced iron uptake (malnutrition, fluid overload), impaired iron storage (inflammation, chronic kidney disease), and iron loss (antiplatelets).
Asunto(s)
Anemia Ferropénica/complicaciones , Líquidos Corporales/fisiología , Insuficiencia Cardíaca/metabolismo , Inflamación/metabolismo , Deficiencias de Hierro , Inhibidores de Agregación Plaquetaria/efectos adversos , Anciano , Anemia Ferropénica/inducido químicamente , Anemia Ferropénica/metabolismo , Arildialquilfosfatasa/metabolismo , Biomarcadores/sangre , Líquidos Corporales/metabolismo , Ingestión de Alimentos/fisiología , Femenino , Insuficiencia Cardíaca/etiología , Insuficiencia Cardíaca/mortalidad , Insuficiencia Cardíaca/fisiopatología , Humanos , Masculino , Persona de Mediana Edad , Péptido Natriurético Encefálico/metabolismo , Readmisión del Paciente/estadística & datos numéricos , Fragmentos de Péptidos/metabolismo , Prevalencia , Pronóstico , Proteínas/provisión & distribución , Insuficiencia Renal Crónica/complicaciones , Insuficiencia Renal Crónica/metabolismo , Volumen Sistólico/fisiología , Fosfatasa Ácida Tartratorresistente/metabolismo , Transferrina/metabolismoRESUMEN
Background: In insulin-resistance, VLDL presents alterations that increase its atherogenic potential. The mechanism by which insulin-resistance promotes the production of altered VLDL is still not completely understood. The aim of this study was to evaluate the relationship between the expression of sterol regulatory element binding protein 1c (SREBP-1c) and of peroxisome proliferator-activated receptor-α (PPAR-α), with the features of composition and size of VLDL in an insulin-resistance rat model induced by a sucrose rich diet (SRD). Methods: The study was conducted on 12 male Wistar rats (180 g) receiving SRD (12 weeks) and 12 controls. Lipid profile, free fatty acids, glucose, and insulin were measured. Lipid content in liver and visceral fat were assessed. Isolated VLDL (d < 1.006 g/ml) was characterized by its chemical composition and size by HPLC. The respective hepatic expression of SREBP-1c and PPAR-α was determined (Western blot). Results: As expected, SRD had elevated triglycerides (TG), free fatty acids and insulin levels, and decreased HDL-cholesterol (p < 0.05), together with augmented hepatic and visceral fat (p < 0.05). SRD showed higher VLDL total mass - with increased TG content - and predominance of large VLDL (p < 0.05). SRD showed an increase in SREBP-1c (precursor and mature forms) and decreased PPAR-α expression (p < 0.045). SREBP-1c forms were positively associated with VLDL total mass (p < 0.04), VLDL-TG% (p < 0.019), and large VLDL% (p < 0.002). On the other hand, PPAR-α correlated negatively with VLDL total mass (p = 0.05), VLDL-TG% (p = 0.005), and large VLDL% (p = 0.002). Conclusions: Insulin-resistance, by coordinated activation of SREBP-1c and reduction of PPAR-α, could promote the secretion of larger and TG over-enriched VLDL particles, with greater atherogenic capacity
Introducción: En la insulinorresistencia, la VLDL presenta alteraciones que aumentan su potencial aterogénico. El mecanismo por el cual la insulinorresistencia promueve la producción de VLDL alteradas aún no se comprende completamente. Objetivo: evaluar la relación entre la expresión de la proteína ligadora de elementos reguladores de esteroles-1c (SREBP-1c) y de los receptores activados por factores de proliferación peroxisomal-α (PPAR-α) con las características de composición y tamaño de VLDL en un modelo animal de insulinorresistencia inducida por dieta rica en sacarosa (DRS). Métodos: Estudiamos 12 ratas macho Wistar (180 g) que recibieron DRS (12 semanas) y 12 controles. Se midieron el perfil lipídico, los ácidos grasos libres, la glucosa y la insulina. Se cuantificaron el contenido lipídico hapático y la grasa visceral. Se caracterizó la VLDL aislada (d < 1,006 g/ml) en composición química y tamaño (HPLC). Se determinó la expresión hepática de SREBP-1c y PPAR-α (Western-blot). Resultados: Esperadamente, el grupo DRS presentó elevación de triglicéridos (TG), ácidos grasos libres e insulina y disminución de colesterol-HDL (p < 0,05), junto con incremento de grasa hepática y visceral (p < 0,05). La DRS mostró una mayor masa total de VLDL con mayor contenido de TG y predominio de VLDL grandes (p < 0,05). DRS presentó expresión incrementada de SREBP-1c (precursor y maduro) y disminuida de PPAR-α (p < 0,045). Ambas formas de SREBP-1c se correlacionaron positivamente con masa total de VLDL (p < 0,04), TG%-VLDL (p < 0,019) y VLDL-grande % (p < 0,002). Mientras que PPAR-α se correlacionó negativamente con masa total de VLDL (p = 0,05), TG %-VLDL (p = 0,005) y VLDL-grande % (p = 0,002). Conclusiones: La insulinorresistencia, mediante una coordinada activación de SREBP-1c y reducción de PPAR-α, promovería la secreción de partículas de VLDL grandes y sobreenriquecidas en TG, con mayor capacidad aterogénica
Asunto(s)
Animales , Ratas , Proyectos de Investigación/normas , Resistencia a la Insulina/genética , Sacarosa en la Dieta/administración & dosificación , Sacarosa en la Dieta/uso terapéutico , Triglicéridos/clasificación , Triglicéridos/deficiencia , Coprofagia/fisiología , Proteínas/administración & dosificación , Proteínas/provisión & distribución , Proyectos de Investigación/estadística & datos numéricos , Resistencia a la Insulina/fisiología , Sacarosa en la Dieta/síntesis química , Sacarosa en la Dieta/aislamiento & purificación , Triglicéridos/provisión & distribución , Triglicéridos/uso terapéutico , Coprofagia/clasificación , Proteínas/análisis , Proteínas/clasificación , Argentina/etnologíaRESUMEN
Background and Objective: The allergenic potential of proteins can be altered under various physicochemical conditions. Glutathione (GSH) is a reducing agent that is used as an antioxidant in food products. We aimed to characterize the natural folding of peach proteins and test the allergenicity of reduced and natural Pru p 3, the major peach allergen. Methods: Pru p 3 was purified from peach, and its conformation was analyzed by means of circular dichroism. Using a thiol fluorescent probe, reduced proteins were detected in fresh peach. GSH-reduced Pru p 3 was tested in vitro for T-cell proliferation and in vivo using skin prick testing. Results: GSH-reduced Pru p 3 produced variable skin prick reactions in peach-allergic patients. The proliferative response of peripheral blood mononuclear cells from allergic patients to reduced Pru p 3 tended to be less intense, whereas secretion of the cytokines IFN-, IL-5, and IL-10 was comparable. In a pool of sera from peach-allergic patients, reduction hardly impaired IgE-binding. Moreover, the stability of reduced Pru p 3 to gastrointestinal digestion was similar to that of the natural form. Conclusions: GSH can at least transiently reduce Pru p 3. We found that the effect of reduction on the allergenicity of Pru p 3 varied. Therefore, as an additive, GSH does not seem to eliminate the risk of reactions for peach-allergic patients (AU)
Antecedentes: El potencial alergénico de las proteínas puede alterarse mediante modificaciones fisicoquímicas. El glutatión (GSH) es un agente reductor utilizado como antioxidante en productos alimentarios. Objetivo: Este estudio pretende caracterizar el plegamiento natural de las proteínas de melocotón y cuantificar la alergenicidad del alérgeno mayor del melocotón, Pru p 3, natural y reducido. Métodos: Para ello, se purificó Pru p 3 y se analizó su conformación mediante dicroismo circular (DC). Mediante el análisis con tiol fluorescente, se detectaron las proteínas reducidas en melocotones frescos. Pru p 3 reducido por GSH fue analizado mediante un ensayo in vitro de proliferación de células T e in vivo mediante prueba cutánea. Resultados: Pru p 3 reducido produjo reacciones variables en las pruebas cutáneas de los pacientes alérgicos a melocotón; sin embargo, su estabilidad a la digestión gastrointestinal fue similar a la de la forma natural. La respuesta proliferativa de las células mononucleares de los pacientes alérgicos frente a Pru p 3 reducido mostró una tendencia a ser inferior, mientras que la secreción de citocinas IFN, IL5 e IL10 fue similar a la producida con la forma natural. La reducción alteró la unión de la IgE a Pru p 3 en un pool de sueros de pacientes alérgicos a melocotón. Conclusión: En conclusión, el glutatión es capaz de reducir Pru p 3, al menos de forma transitoria. En nuestro estudio, la reducción no afectó a la alergenicidad de Pru p 3, de forma que dicho aditivo no parece resolver el riesgo de alergia en pacientes alérgicos a melocotón (AU)
Asunto(s)
Humanos , Masculino , Femenino , Hipersensibilidad a los Alimentos/diagnóstico , Hipersensibilidad a los Alimentos/enzimología , Proteínas/administración & dosificación , Proteínas , Proteínas Ligadas a Lípidos , Pruebas Cutáneas/instrumentación , Pruebas Cutáneas/métodos , Enfermedades Gastrointestinales/diagnóstico , Citocinas , Hipersensibilidad a los Alimentos/metabolismo , Hipersensibilidad a los Alimentos/prevención & control , Proteínas/metabolismo , Proteínas/provisión & distribución , Proteínas Ligadas a Lípidos/provisión & distribución , Pruebas Cutáneas/clasificación , Pruebas Cutáneas , Enfermedades Gastrointestinales/enzimología , Citocinas/genéticaRESUMEN
OBJECTIVE: To compare developmental competency of immature human oocytes matured in vitro in G2 media supplemented by two different protein sources. DESIGN: Retrospective comparative study. SETTING: University Hospital IVF program. PATIENT(S): Sixty-five consecutive women from whom at least one immature oocyte was obtained during 76 cycles of ovarian hyperstimulation and IVF. INTERVENTION(S): G2 media containing human follicular fluid (hFF) (from January to August 2005) or human serum albumin (HSA) (from Sep 2005 to Aug 2006) were used for in vitro maturation of immature oocytes. MAIN OUTCOME MEASURE(S): In vitro maturation rate of immature oocytes and their fertilization rate. RESULT(S): A total of 231 immature oocytes including 107 germinal vesicle (GV) stage and 124 metaphase I (MI) were cultured in vitro and then fertilized by intracytoplasmic sperm injection. The in vitro maturation rate of GV (40.6% vs. 52.0%) and MI oocytes (84.4% vs. 70.0%) and the fertilization rate in GV (76.9% vs. 48.7%) and MI oocytes (70.4% vs. 73.4%) were not different between hFF- and HSA-supplemented G2 media. The cleavage rates of embryos generated from either GV or MI were similar between the two groups, but they were seldom used in embryo transfer. CONCLUSION(S): Developmental competency of immature oocytes was comparable when matured in vitro with G2 media supplemented by either hFF or HSA. Our results suggest that hFF as a protein supplement for human in vitro maturation can be replaced by highly defined HSA.
Asunto(s)
Técnicas de Cultivo de Célula , Líquido Folicular/fisiología , Oocitos/fisiología , Oogénesis/efectos de los fármacos , Proteínas/provisión & distribución , Albúmina Sérica/farmacología , Adulto , Células Cultivadas , Medios de Cultivo/química , Medios de Cultivo/farmacología , Femenino , Fertilización In Vitro/métodos , Líquido Folicular/química , Humanos , Masculino , Persona de Mediana Edad , Embarazo , Estudios Retrospectivos , Albúmina Sérica/químicaRESUMEN
Thirteen clinicaly healthy crossbred (Holstein x Zebu) aged between 20 and 45 months, raised in Rio de Janeiro State, Brazil, were utilized. Samples of blood (2), without anticoagulant, were colected from each animal by jugular vein puncture. It was determined the values for total protein, albumin (commercial kits), globulins and albumin/globulins ratio by mathematics calculation. The observed limits, mean and respective standard deviation in g/dl were: 5.70 to 8.80 and 7.01 more or less 0.65 (total protein); 2.60 to 4.20 and 3.10 more or less 0.36 (albumin); 2.30 to 5.00 and 3.70 more or less 0.59 (globulins) and 0.60 to 3.50 and 2.25 more or less 0.87 (albumin/globulins ratio)
Asunto(s)
Animales , Femenino , Bovinos , Proteínas/provisión & distribuciónRESUMEN
Se apresentam dados do conteúdo de aminoácidos de 15 materiais lignocelulósicos, incluindo águas residuais de oliva (alpechin). Os substratos lignocelulósicos selecionados foram: glicose, celobiose, sacarose, celulose microcristalina, tanino, flobafeno, cortiça, resíduos lignocelulósicos resíduos de açúcares derivados do processo organo-solv, casca de arroz, casca de arroz pré-irradiada, bagaço de laranja, alpechin (5 por cento), alpechin (10 por cento), e alpechin pré-irradiado (10 por cento). Säo apresentadas taxas de alguns aminoacidos de significado nutricional em fontes convencionais de proteínas e aqueles induzidos por diferentes materiais lignocelulósicos. Também säo comparadas as taxas de aminoácidos individuais com aminoácidos essenciais (E/A) e aminoácidos essenciais totais recuperados como nitrogênio total (razäo E/T), juntamente com a escore químico dos aminoácidos essenciais, o que permitiu avaliar a qualidade de cada fonte de carbono utilizada. Essas análises mostram que o bagaço de laranja, alpechin (10 por cento) e cortiça foram os materiais mais econômicos e que resultaram em proteína de maior qualidade
Asunto(s)
Valor Nutritivo , Aminoácidos/biosíntesis , Proteínas de Plantas/análisis , Proteínas/provisión & distribución , Oryza/químicaRESUMEN
O trabalho consiste em uma revisäo de literatura sobre a composiçäo e qualidade nutricional dos resíduos provenientes da separaçäo mecânica de subprodutos da desossa manual de frangos, como dorsos e pescoços