RESUMEN

Bone tissue-derive biomaterials have become of great interest to treat diseases of the skeletal system. Biological scaffolds of demineralized and decellularized extracellular matrices (ECM) have been developed and one of these options are ECM hydrogels derived from bovine bone. Nanomaterials may be able to regulate stem cell differentiation due to their unique physical-chemical properties. The present work aimed to evaluate the osteoinductive effects of ECM hydrogels associated with barium titanate nanoparticles (BTNP) on dental pulp cells derived from exfoliated teeth. The addition of BTNP in the ECM derived hydrogel did not affect cell proliferation and the formation of bone nodules. Furthermore, it increased the expression of bone alkaline phosphatase. The results demonstrated that the nanobiocomposites were able to promote the osteogenic differentiation, even in the absence of chemical inducing factors for osteogenic differentiation. In conclusion, bovine bone ECM hydrogel combined with BTNP presented and increased expression of markers of osteogenic differentiation in the absence of chemical inducing factors.

Asunto(s)

Compuestos de Bario/farmacología , Proliferación Celular/efectos de los fármacos , Matriz Extracelular , Hidrogeles/farmacología , Osteogénesis/efectos de los fármacos , Células Madre/efectos de los fármacos , Titanio/farmacología , Fosfatasa Alcalina/efectos de los fármacos , Fosfatasa Alcalina/genética , Animales , Técnica de Desmineralización de Huesos , Proteína Morfogenética Ósea 2/efectos de los fármacos , Proteína Morfogenética Ósea 2/genética , Proteína Morfogenética Ósea 4/efectos de los fármacos , Proteína Morfogenética Ósea 4/genética , Bovinos , Pulpa Dental/citología , Glicosaminoglicanos/metabolismo , Humanos , Nanopartículas del Metal , Microscopía Electrónica de Rastreo , Osteogénesis/genética , Reología , Espectrometría Raman , Células Madre/metabolismo , Células Madre/ultraestructura , Ingeniería de Tejidos/métodos , Andamios del Tejido