RESUMEN
In the present work, we sought to determine the effects of an individual application of prenatal stress and postnatal stress and their combination on the anxious and locomotor behavior of first-time mother rats. Indeed, three batches of rats were exposed to three types of daily repeated stress: one batch exposed to prenatal restraint stress (SC): 01h a day from day 11 to day 19 of gestation, one batch exposed to postnatal mother prenatal stress (SS): 03h a day from the third to the fourteenth postnatal day (JPN), and one batch exposed to combined restraint stress: 01h from day 11 to day 19 of gestation followed by a postnatal mother separation of 03h a day from the third to fourteenth JPN (CSS); a fourth control batch (T) of female rats that did not undergo any type of stress was also part of our study. At postnatal days 15 and 20, the behavior of the female rats in the four batches was assessed using the Open field Test along with the Plus Maze, and at JPN 30, the female rats were decapitated, the blood of which was collected for plasma adrenocorticotropic hormone (ACTH) level evaluation. Our findings show that all studied behaviors were very highly affected relative to the results of the control batch after individually applying prenatal restraint stress and mother separation. We noticed an intensified anxious behavior in CSS with a significant increase in plasma levels of ACTH.
Asunto(s)
Masculino , Femenino , Animales , Embarazo , Ratas , Conducta Animal , Preñez/psicología , Trastornos Relacionados con Traumatismos y Factores de Estrés/sangre , Ratas WistarRESUMEN
In the present work, we sought to determine the effects of an individual application of prenatal stress and postnatal stress and their combination on the anxious and locomotor behavior of first-time mother rats. Indeed, three batches of rats were exposed to three types of daily repeated stress: one batch exposed to prenatal restraint stress (SC): 01h a day from day 11 to day 19 of gestation, one batch exposed to postnatal mother prenatal stress (SS): 03h a day from the third to the fourteenth postnatal day (JPN), and one batch exposed to combined restraint stress: 01h from day 11 to day 19 of gestation followed by a postnatal mother separation of 03h a day from the third to fourteenth JPN (CSS); a fourth control batch (T) of female rats that did not undergo any type of stress was also part of our study. At postnatal days 15 and 20, the behavior of the female rats in the four batches was assessed using the Open field Test along with the Plus Maze, and at JPN 30, the female rats were decapitated, the blood of which was collected for plasma adrenocorticotropic hormone (ACTH) level evaluation. Our findings show that all studied behaviors were very highly affected relative to the results of the control batch after individually applying prenatal restraint stress and mother separation. We noticed an intensified anxious behavior in CSS with a significant increase in plasma levels of ACTH.(AU)
Asunto(s)
Animales , Masculino , Femenino , Embarazo , Ratas , Conducta Animal , Trastornos Relacionados con Traumatismos y Factores de Estrés/sangre , Preñez/psicología , Ratas WistarRESUMEN
The objective of the study was to identify the different behavior of Etawah Grade does in early and late pregnancy period. This research using 14 head of Etawah Grade does in pregnant conditions with an age of fewer than 2 months as the early pregnancy group and gestational age of 4 to 5 months as the late pregnancy group in Farm of FacultyAnimal Science, Universitas Gadjah Mada. The method usedwas direct observation. The goats were observed for 24 hours. The data observed were consisted of activities including time and frequency of feeding, ruminating, standing, lying, urinating and defecating. Data behavior of goats was analyzed using T-test. The results showed that the temperature and humidity of the study ranged from 25 to 31 ºC and 65 to 90%, respectively. The heart rate frequency in late pregnancy higher (P < 0.05) than in early pregnancy conditions. There were significant differences (P < 0.05) on the behavior of early and late pregnancy of Etawah Grade does, in terms of frequency of feeding (17.11±1.33 and 13.61±2.85 times/day), and average in once feeding (10.24±1.64 and 12.88±2.36 minutes), standing duration (15.05±0.83 and 11.45±1.34 hours/day), laying duration (9.01±0.71 and 12.54±1.34 hours/day), average in once laying (31.32±3.6 and 47.89±5.68), and frequency of urination (6.56±0.34 and 9.55±2.01). The conclusion of the study is the Etawah Grade does during early pregnancy and late pregnancy has different behavior, especially on the frequency of feeding, average on once feeding, standing duration, time and average of once lying, and frequency of urination.
Asunto(s)
Femenino , Animales , Embarazo , Cabras/fisiología , Cabras/psicología , Conducta Animal , Preñez/psicologíaRESUMEN
The objective of the study was to identify the different behavior of Etawah Grade does in early and late pregnancy period. This research using 14 head of Etawah Grade does in pregnant conditions with an age of fewer than 2 months as the early pregnancy group and gestational age of 4 to 5 months as the late pregnancy group in Farm of FacultyAnimal Science, Universitas Gadjah Mada. The method usedwas direct observation. The goats were observed for 24 hours. The data observed were consisted of activities including time and frequency of feeding, ruminating, standing, lying, urinating and defecating. Data behavior of goats was analyzed using T-test. The results showed that the temperature and humidity of the study ranged from 25 to 31 ºC and 65 to 90%, respectively. The heart rate frequency in late pregnancy higher (P < 0.05) than in early pregnancy conditions. There were significant differences (P < 0.05) on the behavior of early and late pregnancy of Etawah Grade does, in terms of frequency of feeding (17.11±1.33 and 13.61±2.85 times/day), and average in once feeding (10.24±1.64 and 12.88±2.36 minutes), standing duration (15.05±0.83 and 11.45±1.34 hours/day), laying duration (9.01±0.71 and 12.54±1.34 hours/day), average in once laying (31.32±3.6 and 47.89±5.68), and frequency of urination (6.56±0.34 and 9.55±2.01). The conclusion of the study is the Etawah Grade does during early pregnancy and late pregnancy has different behavior, especially on the frequency of feeding, average on once feeding, standing duration, time and average of once lying, and frequency of urination.(AU)
Asunto(s)
Animales , Femenino , Embarazo , Conducta Animal , Cabras/fisiología , Cabras/psicología , Preñez/psicologíaRESUMEN
Abstract Background: Prenatal stress may increase risk of developing cardiovascular disorders in adulthood. The cardiotoxic effects of catecholamines are mediated via prolonged adrenergic receptor stimulation and increased oxidative stress upon their degradation by monoamine oxidase A (MAO-A). Objectives: We investigated long-term effects of prenatal stress on β (1, 2, 3) adrenergic receptors and MAO-A gene expression in the hearts of adult rat offspring. Methods: Pregnant rats were exposed to unpredictable mild stress during the third week of gestation. RNA was isolated from left ventricular apex and base of adult offspring. Quantitative PCR was used to measure gene expression in collected ventricular tissue samples. The level of significance was set to p < 0.05. Results: β3 adrenergic receptor mRNA was undetectable in rat left ventricle. β1 adrenergic receptor was the predominantly expressed subtype at the apical and basal left ventricular myocardium in the control females. Male offspring from unstressed mothers displayed higher apical cardiac β1 than β2 adrenergic receptor mRNA levels. However, β1 and β2 adrenergic receptor mRNAs were similarly expressed at the ventricular basal myocardium in males. Unlike males, prenatally stressed females exhibited decreased β1 adrenergic receptor mRNA expression at the apical myocardium. Prenatal stress did not affect cardiac MAO-A gene expression. Conclusions: Collectively, our results show that prenatal stress may have exerted region- and sex-specific β1 and β2 adrenergic receptor expression patterns within the left ventricle.
Resumo Fundamento: Estresse pré-natal pode aumentar os riscos de desenvolver doenças cardiovasculares na idade adulta. Os efeitos cardiotóxicos de catecolaminas são mediados pela estimulação prolongada dos receptores adrenérgicos e pelo aumento do estresse oxidativo após sua degradação pela monoamina oxidase A (MAO-A). Objetivos: Investigamos os efeitos a longo prazo de estresse pré-natal nos receptores β (1, 2, 3) adrenérgicos e na expressão do gene MAO-A nos corações da prole adulta de ratos. Método: Ratas prenhes foram expostas a estresse crônico moderado imprevisível durante a terceira semana de gestação. O RNA foi isolado do ápice e da base do ventrículo esquerdo da prole adulta. Utilizou-se PCR quantitativa em tempo real para medir a expressão gênica nas amostras de tecido ventricular coletadas. O nível de significância foi estabelecido em p < 0,05. Resultados: Foi indetectável o mRNA do receptor adrenérgico β3 no ventrículo esquerdo dos ratos. O receptor adrenérgico β1 foi o subtipo mais expresso no miocárdio ventricular esquerdo apical e basal nas fêmeas controle. A prole masculina das mães não estressadas apresentou níveis cardíacos apicais de mRNA do receptor adrenérgico β1 mais altos do que os de β2. Porém, mRNAs dos receptores adrenérgicos β1 e β2 foram expressos de forma semelhante no miocárdio basal ventricular na prole masculina em geral. Ao contrário da prole masculina, a prole feminina exposta ao estresse pré-natal exibiu uma expressão diminuída do mRNA do receptor adrenérgico β1 no miocárdio apical. O estresse pré-natal não afetou a expressão gênica de MAO-A cardíaca. Conclusões: Coletivamente, nossos resultados mostram que estresse pré-natal pode ter exercido padrões de expressão região- e sexo-específica dos receptores adrenérgicos β1 e β2 no ventrículo esquerdo.
Asunto(s)
Animales , Femenino , Embarazo , Efectos Tardíos de la Exposición Prenatal/metabolismo , Estrés Psicológico/metabolismo , Preñez/psicología , Receptores Adrenérgicos beta/análisis , Monoaminooxidasa/análisis , Miocardio/metabolismo , Efectos Tardíos de la Exposición Prenatal/psicología , Valores de Referencia , Estrés Psicológico/genética , Factores de Tiempo , ARN Mensajero/análisis , Enfermedades Cardiovasculares/etiología , Enfermedades Cardiovasculares/metabolismo , Enfermedades Cardiovasculares/psicología , Expresión Génica , Factores Sexuales , Receptores Adrenérgicos beta/genética , Ratas Wistar , Hormona Adrenocorticotrópica/sangre , Reacción en Cadena en Tiempo Real de la Polimerasa , Ventrículos Cardíacos/metabolismo , Monoaminooxidasa/genética , Madres/psicologíaRESUMEN
BACKGROUND: Prenatal stress may increase risk of developing cardiovascular disorders in adulthood. The cardiotoxic effects of catecholamines are mediated via prolonged adrenergic receptor stimulation and increased oxidative stress upon their degradation by monoamine oxidase A (MAO-A). OBJECTIVES: We investigated long-term effects of prenatal stress on ß (1, 2, 3) adrenergic receptors and MAO-A gene expression in the hearts of adult rat offspring. METHODS: Pregnant rats were exposed to unpredictable mild stress during the third week of gestation. RNA was isolated from left ventricular apex and base of adult offspring. Quantitative PCR was used to measure gene expression in collected ventricular tissue samples. The level of significance was set to p < 0.05. RESULTS: ß3 adrenergic receptor mRNA was undetectable in rat left ventricle. ß1 adrenergic receptor was the predominantly expressed subtype at the apical and basal left ventricular myocardium in the control females. Male offspring from unstressed mothers displayed higher apical cardiac ß1 than ß2 adrenergic receptor mRNA levels. However, ß1 and ß2 adrenergic receptor mRNAs were similarly expressed at the ventricular basal myocardium in males. Unlike males, prenatally stressed females exhibited decreased ß1 adrenergic receptor mRNA expression at the apical myocardium. Prenatal stress did not affect cardiac MAO-A gene expression. CONCLUSIONS: Collectively, our results show that prenatal stress may have exerted region- and sex-specific ß1 and ß2 adrenergic receptor expression patterns within the left ventricle.
Asunto(s)
Monoaminooxidasa/análisis , Miocardio/metabolismo , Preñez/psicología , Efectos Tardíos de la Exposición Prenatal/metabolismo , Receptores Adrenérgicos beta/análisis , Estrés Psicológico/metabolismo , Hormona Adrenocorticotrópica/sangre , Animales , Enfermedades Cardiovasculares/etiología , Enfermedades Cardiovasculares/metabolismo , Enfermedades Cardiovasculares/psicología , Femenino , Expresión Génica , Ventrículos Cardíacos/metabolismo , Monoaminooxidasa/genética , Madres/psicología , Embarazo , Efectos Tardíos de la Exposición Prenatal/psicología , ARN Mensajero/análisis , Ratas Wistar , Reacción en Cadena en Tiempo Real de la Polimerasa , Receptores Adrenérgicos beta/genética , Valores de Referencia , Factores Sexuales , Estrés Psicológico/genética , Factores de TiempoRESUMEN
In rats, mating at postpartum estrus and delayed dispersal of the young would result in the overlapping of two different-age litters. As a consequence, newborn pups' early experience will include not only that acquired during the interaction with the mother and age-matched littermates, but also with older siblings. As early-life experience modulates rodents' brain function, behavior and reproduction, we aimed to assess how changes in the early environment provoked by the overlapping of litters would affect emotionality, stress response and reproductive functions of male and female pups during adulthood. Results showed that both male and female overlapped reared pups exhibited a reduced behavioral inhibition in the open field test during adulthood. In addition, overlapped reared adult females, but not males, showed a blunted corticosterone response to an acute stressor during diestrus and a reduction in sexual behavior. In summary, natural changes in early experience provoked by the overlapping of litters, long-term modulate affective and reproductive behaviors, and the endocrine stress response in a sex dimorphic manner.
Asunto(s)
Nivel de Alerta/fisiología , Emociones/fisiología , Tamaño de la Camada/fisiología , Preñez/fisiología , Relaciones entre Hermanos , Medio Social , Estrés Psicológico/complicaciones , Animales , Corticosterona/sangre , Diestro/fisiología , Conducta Exploratoria/fisiología , Femenino , Inhibición Psicológica , Masculino , Actividad Motora/fisiología , Ovulación/fisiología , Embarazo , Preñez/psicología , Ratas , Ratas Wistar , Caracteres Sexuales , Conducta Sexual Animal/fisiología , Espermatogénesis/fisiología , Estrés Psicológico/fisiopatologíaRESUMEN
Several studies have shown the influence of the oestrous cycle on anxiety levels and the important role of progesterone in this effect. The metabolism of this steroid hormone yields neuroactive steroids among them allopregnanolone (alloP) and allotetrahydrodeoxycorticosterone (alloTHDOC), which bind to GABAA receptors and have anxiolytic effects. Considering that during pregnancy there is an increase in levels of both progesterone and its metabolites, the main objectives of this work were: (1) to assess changes in anxiety levels during pregnancy and (2) to verify the role of alloP and alloTHDOC in this process using finasteride, an inhibitor of 5alpha-reductase, the enzyme responsible for their synthesis. The results showed a significant reduction in anxiety levels on the 19th day of pregnancy, which was reversed by finasteride, suggesting a role for alloP and alloTHDOC in the anxiolytic process.
Asunto(s)
Inhibidores de 5-alfa-Reductasa , Ansiedad/prevención & control , Corticosterona/análogos & derivados , Inhibidores Enzimáticos/farmacología , Finasterida/farmacología , Preñez/psicología , Pregnanolona/fisiología , Animales , Corticosterona/fisiología , Femenino , Embarazo , Ratas , Ratas WistarRESUMEN
This study demonstrates changes in experimental anxiety assessed in the black and white paradigm during various reproductive states of female rats. Low levels of experimental anxiety were observed during late proestrus and on day 17 of gestation, stages related to high progesterone (P) levels. In estrus, metestrus, diestrus and on day 21 of gestation, stages characterized by low P concentrations, high levels of experimental anxiety, similar to those exhibited by ovariectomized females, were found. No changes in experimental anxiety were observed on day 8 of lactation compared to ovariectomized females. These data are discussed from the standpoint of the putative anxiolytic-like effect of progestins.
Asunto(s)
Ansiedad/fisiopatología , Ciclo Estral/fisiología , Lactancia/fisiología , Preñez/psicología , Análisis de Varianza , Animales , Conducta Animal , Modelos Animales de Enfermedad , Femenino , Aprendizaje por Laberinto/fisiología , Actividad Motora/fisiología , Ovariectomía/métodos , Embarazo , Ratas , Ratas Wistar , Tiempo de Reacción/fisiología , Reproducibilidad de los ResultadosRESUMEN
RATIONALE: In rats, several behavioral changes occurring during pregnancy could be due to the presence of progesterone; some of them may be analyzed in the differential reinforcement of the low-rate 72 s task (DRL-72 s), which is designed for testing the antidepressant profile of drugs. OBJECTIVES: The aim of the present study was to analyze the behavior of pregnant rats or ovariectomized rats receiving exogenous progesterone in the DRL-72 s task. HYPOTHESIS: During pregnancy, rats will obtain a high number of reinforcers in the DRL-72 s task. METHODS: Pregnant rats or rats after delivery were tested in the DRL-72 s task at the 3rd, 7th, 14th, 17th, and 20th days. Control rats previously trained in the DRL-72 s task were ovariectomized; after recuperation, they received saline (0.9%, i.p.), clomipramine (1.25 mg/kg, i.p.), or desipramine (2.14 mg/kg, i.p.) for 28 days, and they were tested in the DRL-72 s task. In a second series of experiments, ovariectomized rats received vehicle or progesterone (0.5, 1.0, 2.0, 4.0 mg/kg, s.c.), and they were submitted to the DRL-72 s task. Locomotion was evaluated in the open field test. RESULTS: Pregnant rats tested at the 14th and 17th day and ovariectomized rats receiving progesterone or two tricyclic antidepressants obtained a higher number of reinforcers and a cohesive rightward shift in inter-response time distributions than those rats evaluated after delivery in the DRL-72 s task. A lower locomotion was observed only at the end of pregnancy. CONCLUSIONS: Antidepressant-like effects of pregnancy and progesterone were found in Wistar rats as measured in the DRL-72 s task.