RESUMEN
In the present study, potentiality of endophytic microorganisms such as Rigidiporus vinctus AAU EF, Trichoderma reesei UH EF, and Sphingobacterium tabacisoli UH EB in the management of panama wilt and growth promotion of banana was assessed through artificial inoculation. During the study, a total of 220 bacterial and 110 fungal endophytes were isolated from root, pseudostem, and leaf samples of banana, and they were evaluated against Fusarium oxysporum f. sp cubense causing panama wilt. Out of total 330 bacterial and fungal endophytes, only five endophytes exhibited antagonism against Fusarium oxysporum f. sp cubense, out of which only three isolates, namely Trichoderma reesei UH EF, Rigidiporus vinctus AAU EF, and Sphingobacterium tabacisoli UH EB, produced indole acetic acid, siderophore, and hydrogen cyanide, except one bacterial strain Sphingobacterium tabacisoli UH EB which does not produce hydrogen cyanide. Furthermore, these three endophytes were identified through cultural and morphological characteristics as well as by the sequencing internal transcribed spacer (ITS) and 16S rRNA gene sequences analysis for bacteria, respectively. The response of host plant to endophyte inoculation was assessed by measuring the change in four growth parameters; plant height, pseudo stem girth (diameter), number of roots, and total number of leaves. The application of endophytes, irrespective of isolate and treatment type promoted the overall growth of the plant growth when compared with diseased plants with significant higher values recorded for all parameters assessed. The endophytes reported as growth promoters were found to have significant inhibition effect on Foc which can evidenced with lowest AUDPC values and epidemic rate at 99.09 units2 and 0.02 unit/day, respectively.
Asunto(s)
Endófitos , Fusarium , Musa , Enfermedades de las Plantas , Endófitos/fisiología , Fusarium/fisiología , Hypocreales/fisiología , Interacciones Microbianas/fisiología , Musa/microbiología , Enfermedades de las Plantas/microbiología , Enfermedades de las Plantas/prevención & control , Polyporales/fisiología , ARN Ribosómico 16S/genética , Sphingobacterium/fisiologíaRESUMEN
This is a review of the available knowledge on nuclear behavior of the mycelium within polypore genera (Agaricomycetes, Basidiomycota). Information on 68 genera showed that nuclear behavior is a distinct and consistent feature at genus level and can be coupled with phylogenetic differentiation. The sole exception was found in Polyporus, where different species with normal, heterocytic and astatocoenocytic nuclear behaviors were found. Of the 68 genera treated 41 (60.3%) displayed a normal nuclear behavior, nine (13.2%) were heterocytic, nine (13.2%) were astatocoenocytic and another eight (11.8%) were holocoenocytic. In 95% of the genera a unique compatibility system was found, with the exceptions of Antrodia, which includes both homothallic and bipolar species all associated with a normal nuclear behavior, and Spongipellis, in which bipolar and tetrapolar species are found, all displaying an astatocoenocytic nuclear behavior. Normal and heterocytic nuclear behaviors were associated mostly with tetrapolarity, astatocoenocity was associated mostly with bipolarity, and holocoenocity was associated with either bipolarity or purported homothallism. The combination of nuclear behavior with mating system and brown or white rot capability appeared as a strong feature characterizing and distinguishing the genera of polypores, each combination being valuable to differentiate between apparently related genera, as is supported by phylogenetic studies. Several examples are presented to support this idea, as well as the cases of species that are problematic to this concept. Poroid genera of Hymenochaetaceae were treated apart because of the lack of knowledge regarding their nuclear behavior. In addition new information on the sexuality and/or nuclear behavior of 15 polyporoid taxa is given.
Asunto(s)
Núcleo Celular/fisiología , Polyporales/fisiología , Núcleo Celular/genética , ADN de Hongos/genética , ADN Ribosómico/genética , ADN Espaciador Ribosómico/genética , Genes del Tipo Sexual de los Hongos , Micelio/genética , Micelio/fisiología , Filogenia , Polyporales/clasificación , Polyporales/genéticaRESUMEN
The following names are proposed for species of Lentaria new to science: L. boletosporioides from New Zealand with stout basidiomata and boletoid basidiospores; L. caribbeana from Caribbean islands and circum-Caribbean landmasses, with large basidiomata and large basidiospores; L. glaucosiccescens from New Zealand, with basidiomata turning grey-green upon drying; and L. rionegrensis from Argentina, with densely branched, ruddy basidiomata. Lentaria javanica nom. nov. is proposed to substitute for Clavaria compressa, a later homonym. Basidiospore statistics indicate infraspecific differentiation within L. surculus and two morphological variants are described. Lentaria surculus and L. byssiseda exhibit tetrapolar mating systems, and the two species are sexually interincompatible.