RESUMEN
The aim of this study was to determine the efficiency of Dugesia dorotocephala on Methyl parathion removal. An initial concentration of 1.25 microg mL(-1) of MeP was used to evaluate the removal capacity of planarian. A first-order removal kinetics was obtained with a disappearance rate constant (k(r)) of 0.49 days(-1) and 69% efficiency on contaminant removal. This is significantly different (p < 0.5) from the degradation occurring in control systems, leading us to conclude that D. dorotocephala effectively removes MeP from contaminated water.
Asunto(s)
Inhibidores de la Colinesterasa/metabolismo , Insecticidas/metabolismo , Metil Paratión/metabolismo , Planarias/metabolismo , Contaminantes Químicos del Agua/metabolismo , Acetilcolinesterasa/análisis , Acetilcolinesterasa/metabolismo , Animales , Biodegradación Ambiental , Biomarcadores , Inhibidores de la Colinesterasa/química , Inhibidores de la Colinesterasa/toxicidad , Insecticidas/química , Insecticidas/toxicidad , Cinética , Dosificación Letal Mediana , Metil Paratión/química , Metil Paratión/toxicidad , Planarias/química , Planarias/efectos de los fármacos , Contaminantes Químicos del Agua/química , Contaminantes Químicos del Agua/toxicidadRESUMEN
The presence and distribution of serotonin (5-hydroxytryptamine, or 5-HT) in the head region of the land planarian Bipalium kewense has been investigated by an indirect immunofluorescence technique combined with confocal scanning laser microscopy (CSLM), and also by immunogold labeling at ultrastructural level. Serotonin immunoreactivity (IR) was restricted to elements of the nervous system, such as the cerebral ganglion, and the peripheral nerve net. Most of 5-HT-immunoreactive neurons are at the periphery of the brain; they were identified as unipolar, bipolar, and multipolar neurons. The ultrastructural results using immunogold labeling confirm the location of 5-HT within electron-dense vesicles (50-120 nm in diameter), clustered both in the cell bodies and in their processes. The intense 5-HT-IR herein demonstrated for B. kewense adds new data to the poorly studied nervous system of land planarians.