RESUMEN
A case of 26 year-old female with peripartum cardiomyopathy, acute pancreatitis, periadipose tissue inflammation due to unknown cause and multiple organ dysfunction syndrome complication is presented.
Asunto(s)
Enfermedades Autoinmunes/inmunología , Cardiomiopatías/inmunología , Insuficiencia Multiorgánica/inmunología , Pancreatitis/inmunología , Panicum/inmunología , Trastornos Puerperales/inmunología , Adulto , Femenino , HumanosRESUMEN
Resistance gene homologs (RGHs) were isolated from the switchgrass variety Alamo by a combination of polymerase chain reaction and expressed sequence tag (EST) database mining. Fifty-eight RGHs were isolated by polymerase chain reaction and 295 RGHs were identified in 424,545 switchgrass ESTs. Four nucleotide binding site--leucine-rich repeat RGHs were selected to investigate RGH haplotypic diversity in seven switchgrass varieties chosen for their representation of a broad range of the switchgrass germplasm. Lowland and upland ecotypes were found to be less similar, even from nearby populations, than were more distant populations with similar growth environments. Most (83.5%) of the variability in these four RGHs was found to be attributable to the within-population component. The difference in nucleotide diversity between and within populations was observed to be small, whereas this diversity is maintained to similar degrees at both population and ecotype levels. The results also revealed that the analyzed RGHs were under positive selection in the studied switchgrass accessions. Intragenic recombination was detected in switchgrass RGHs, thereby demonstrating an active genetic process that has the potential to generate new resistance genes with new specificities that might act against newly-arising pathogen races.
Asunto(s)
Resistencia a la Enfermedad/genética , Genes de Plantas/genética , Variación Genética , Panicum/genética , Panicum/inmunología , Homología de Secuencia de Ácido Nucleico , Secuencia de Bases , Sitios de Unión/genética , Clonación Molecular , Análisis por Conglomerados , Secuencia Conservada/genética , Bases de Datos de Ácidos Nucleicos , Ecotipo , Etiquetas de Secuencia Expresada , Haplotipos/genética , Datos de Secuencia Molecular , Motivos de Nucleótidos/genética , Nucleótidos/genética , Filogenia , Análisis de Componente Principal , Recombinación Genética/genética , Selección GenéticaRESUMEN
Celiac disease (CD) is a gluten-sensitive enteropathy with an immune basis. We established the immune reactivity of the alcohol-soluble fraction from two minor cereals (tef and millet) and two pseudocereals (amaranth and quinoa) which are believed to be nontoxic based on taxonomy. Grains were examined in intestinal T-cell lines (iTCLs), cultures of duodenal explants from HLA-DQ2(+) CD patients and HLA-DQ8 transgenic mice for signs of activation. Our data indicated that tef, millet, amaranth, and quinoa did not show any immune cross-reactivity toward wheat gliadin, and therefore confirming their safety in the diet of CD patients.
Asunto(s)
Enfermedad Celíaca/etiología , Grano Comestible/inmunología , Proteínas de Plantas/inmunología , Alcoholes , Amaranthus/inmunología , Secuencia de Aminoácidos , Animales , Chenopodium quinoa/inmunología , Reacciones Cruzadas , Glútenes , Humanos , Interferón gamma/biosíntesis , Ratones , Ratones Transgénicos , Datos de Secuencia Molecular , Panicum/inmunología , Extractos Vegetales/inmunología , Proteínas de Plantas/química , Alineación de SecuenciaRESUMEN
BACKGROUND: Millet has been reported to induce not very frequent but severe anaphylactic reactions following ingestion. Seven individuals who all kept cage birds experienced allergic reactions after ingestion of millet-containing food. METHODS: We investigated the immunoglobulin E (IgE)-reactivity of these individuals to millet employing immunoblotting, RAST and skin prick tests. As the sensitization possibly occurred via the inhalant route we investigated millet-specific IgE levels of 16 additional sera from bird keepers with proven atopy, in retrospect. RESULTS: All patients who had experienced reactions after ingestion of millet displayed millet-specific IgE. Sixty-three percent of the atopic bird keepers possessed millet-specific IgE. By means of immunoblotting three major allergens in millet extract were detected. CONCLUSIONS: Our results indicate that millet plays an important role as inhalant allergen for atopic bird keepers. A sensitization to millet may subsequently also elicit food allergy.