RESUMEN

Anthocyanidin synthase (ANS), a 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase, catalyzes the penultimate step in anthocyanin biosynthesis, from leucoanthocyanidins to anthocyanidins, the first colored compound in the anthocyanin pathway. In this study, a full-length, 1427-bp long cDNA named RnANS1, which is homologous to the anthocyanidin synthase gene, was cloned from blackcurrant using a homologous cloning strategy. RnANS1 is highly homologous to other plant ANS genes at both the nucleotide and amino acid sequence levels. The deduced protein contains domains conserved in the 2OG and Fe(II)-dependent oxygenase, and is phylogenetically closely related to Paeonia suffruticosa and Paeonia lactiflora. The expression of RnANS1 was upregulated during fruit maturation, and correlated with the accumulation of anthocyanins and soluble carbohydrates in the fruit. Further characterization of the structure and expression patterns of RnANS1 will clarify our understanding of anthocyanin biosynthesis in blackcurrant, and support the development of molecular approaches to manipulate anthocyanin production in this plant.

Asunto(s)

Frutas/genética , Perfilación de la Expresión Génica , Oxigenasas/genética , Proteínas de Plantas/genética , Ribes/genética , Secuencia de Aminoácidos , Antocianinas/metabolismo , Carbohidratos/análisis , Clonación Molecular , ADN Complementario/química , ADN Complementario/genética , Frutas/crecimiento & desarrollo , Frutas/metabolismo , Regulación del Desarrollo de la Expresión Génica , Regulación Enzimológica de la Expresión Génica , Regulación de la Expresión Génica de las Plantas , Datos de Secuencia Molecular , Oxigenasas/clasificación , Oxigenasas/metabolismo , Filogenia , Proteínas de Plantas/metabolismo , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Ribes/crecimiento & desarrollo , Ribes/metabolismo , Análisis de Secuencia de ADN , Homología de Secuencia de Aminoácido