RESUMEN
PURPOSE: To assess the action of vitamin C on the expression of 84 oxidative stress related-genes in cultured skin fibroblasts from burn patients. METHODS: Skin samples were obtained from ten burn patients. Human primary fibroblasts were isolated and cultured to be distributed into 2 groups: TF (n = 10, fibroblasts treated with vitamin C) and UF (n = 10, untreated fibroblasts). Gene expression analysis using quantitative polymerase chain reaction array was performed for comparisons between groups. RESULTS: The comparison revealed 10 upregulated genes as follows: arachidonate 12-lipoxygenase (ALOX12), 24-dehydrocholesterol reductase (DHCR24), dual oxidase 1 (DUOX1), glutathione peroxidase 2 (GPX2), glutathione peroxidase 5 (GPX5), microsomal glutathione S-transferase 3 (MGST3), peroxiredoxin 4 (PRDX4), phosphatidylinositol-3,4,5-trisphosphate dependent Rac exchange factor 1 (P-REX1), prostaglandin-endoperoxide synthase 1 (PTGS1), and ring finger protein 7 (RNF7). CONCLUSION: Cultured fibroblasts obtained from burn patients and treated with vitamin C resulted in 10 differentially expressed genes, all overexpressed, with DUOX1, GPX5, GPX2 and PTGS1 being of most interest.
Asunto(s)
Ácido Ascórbico/farmacología , Quemaduras/patología , Fibroblastos/efectos de los fármacos , Fibroblastos/patología , Expresión Génica/efectos de los fármacos , Estrés Oxidativo/efectos de los fármacos , Adulto , Araquidonato 12-Lipooxigenasa/análisis , Araquidonato 12-Lipooxigenasa/efectos de los fármacos , Quemaduras/tratamiento farmacológico , Células Cultivadas , Estudios Transversales , Ciclooxigenasa 1/análisis , Ciclooxigenasa 1/efectos de los fármacos , Oxidasas Duales/análisis , Oxidasas Duales/efectos de los fármacos , Femenino , Glutatión Peroxidasa/análisis , Glutatión Peroxidasa/efectos de los fármacos , Glutatión Transferasa/análisis , Glutatión Transferasa/efectos de los fármacos , Factores de Intercambio de Guanina Nucleótido/análisis , Factores de Intercambio de Guanina Nucleótido/efectos de los fármacos , Humanos , Masculino , Proteínas del Tejido Nervioso/análisis , Proteínas del Tejido Nervioso/efectos de los fármacos , Oxidorreductasas actuantes sobre Donantes de Grupo CH-CH/análisis , Oxidorreductasas actuantes sobre Donantes de Grupo CH-CH/efectos de los fármacos , Peroxirredoxinas/análisis , Peroxirredoxinas/efectos de los fármacos , Reacción en Cadena en Tiempo Real de la Polimerasa , Valores de Referencia , Reproducibilidad de los Resultados , Piel/efectos de los fármacos , Piel/patología , Estadísticas no Paramétricas , Ubiquitina-Proteína Ligasas/análisis , Ubiquitina-Proteína Ligasas/efectos de los fármacos , Adulto JovenRESUMEN
Abstract Purpose: To assess the action of vitamin C on the expression of 84 oxidative stress related-genes in cultured skin fibroblasts from burn patients. Methods: Skin samples were obtained from ten burn patients. Human primary fibroblasts were isolated and cultured to be distributed into 2 groups: TF (n = 10, fibroblasts treated with vitamin C) and UF (n = 10, untreated fibroblasts). Gene expression analysis using quantitative polymerase chain reaction array was performed for comparisons between groups. Results: The comparison revealed 10 upregulated genes as follows: arachidonate 12-lipoxygenase (ALOX12), 24-dehydrocholesterol reductase (DHCR24), dual oxidase 1 (DUOX1), glutathione peroxidase 2 (GPX2), glutathione peroxidase 5 (GPX5), microsomal glutathione S-transferase 3 (MGST3), peroxiredoxin 4 (PRDX4), phosphatidylinositol-3,4,5-trisphosphate dependent Rac exchange factor 1 (P-REX1), prostaglandin-endoperoxide synthase 1 (PTGS1), and ring finger protein 7 (RNF7). Conclusion: Cultured fibroblasts obtained from burn patients and treated with vitamin C resulted in 10 differentially expressed genes, all overexpressed, with DUOX1, GPX5, GPX2 and PTGS1 being of most interest.